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P28002 (COMT1_MEDSA) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Caffeic acid 3-O-methyltransferase

Short name=CAOMT
Short name=COMT
EC=2.1.1.68
Alternative name(s):
S-adenosysl-L-methionine:caffeic acid 3-O-methyltransferase
OrganismMedicago sativa (Alfalfa)
Taxonomic identifier3879 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeTrifolieaeMedicago

Protein attributes

Sequence length365 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the conversion of caffeic acid to ferulic acid and of 5-hydroxyferulic acid to sinapic acid. The resulting products may subsequently be converted to the corresponding alcohols that are incorporated into lignins.

Catalytic activity

S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate = S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate.

Pathway

Aromatic compound metabolism; phenylpropanoid biosynthesis.

Subunit structure

Homodimer. Ref.2

Tissue specificity

More abundant in roots and stems.

Induction

By infection, plant wounding, or elicitor treatment of cell cultures.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O-methyltransferase family. COMT subfamily.

Ontologies

Keywords
   Biological processLignin biosynthesis
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical term3D-structure
Gene Ontology (GO)
   Biological_processlignin biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functioncaffeate O-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 365365Caffeic acid 3-O-methyltransferase
PRO_0000063204

Regions

Region130 – 1367Substrate binding
Region162 – 18019Substrate binding

Sites

Active site2691Proton acceptor
Binding site2081S-adenosyl-L-methionine; via carbonyl oxygen
Binding site2311S-adenosyl-L-methionine
Binding site2511S-adenosyl-L-methionine
Binding site2521S-adenosyl-L-methionine; via amide nitrogen
Binding site2651S-adenosyl-L-methionine

Secondary structure

............................................................ 365
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P28002 [UniParc].

Last modified August 1, 1992. Version 1.
Checksum: C14B0D75F979C6B6

FASTA36539,946
        10         20         30         40         50         60 
MGSTGETQIT PTHISDEEAN LFAMQLASAS VLPMILKSAL ELDLLEIIAK AGPGAQISPI 

        70         80         90        100        110        120 
EIASQLPTTN PDAPVMLDRM LRLLACYIIL TCSVRTQQDG KVQRLYGLAT VAKYLVKNED 

       130        140        150        160        170        180 
GVSISALNLM NQDKVLMESW YHLKDAVLDG GIPFNKAYGM TAFEYHGTDP RFNKVFNKGM 

       190        200        210        220        230        240 
SDHSTITMKK ILETYTGFEG LKSLVDVGGG TGAVINTIVS KYPTIKGINF DLPHVIEDAP 

       250        260        270        280        290        300 
SYPGVEHVGG DMFVSIPKAD AVFMKWICHD WSDEHCLKFL KNCYEALPDN GKVIVAECIL 

       310        320        330        340        350        360 
PVAPDSSLAT KGVVHIDVIM LAHNPGGKER TQKEFEDLAK GAGFQGFKVH CNAFNTYIME 


FLKKV 

« Hide

References

[1]"Stress responses in alfalfa (Medicago sativa L). X. Molecular cloning and expression of S-adenosyl-L-methionine:caffeic acid 3-O-methyltransferase, a key enzyme of lignin biosynthesis."
Gowri G., Bugos R.C., Campbell W.H., Maxwell C.A., Dixon R.A.
Plant Physiol. 97:7-14(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Apollo.
[2]"Structural basis for the modulation of lignin monomer methylation by caffeic acid/5-hydroxyferulic acid 3/5-O-methyltransferase."
Zubieta C., Kota P., Ferrer J.-L., Dixon R.A., Noel J.P.
Plant Cell 14:1265-1277(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) IN COMPLEX WITH SUBSTRATES, SUBUNIT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M63853 mRNA. Translation: AAB46623.1.
PIRT09673.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1KYWX-ray2.40A/C/F1-365[»]
1KYZX-ray2.20A/C/E1-365[»]
ProteinModelPortalP28002.
SMRP28002. Positions 5-365.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

ProMEXP28002.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00711.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
3.40.50.150. 1 hit.
InterProIPR016461. COMT.
IPR001077. O_MeTrfase_2.
IPR012967. Plant_MeTrfase_dimerisation.
IPR029063. SAM-dependent_MTases-like.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF08100. Dimerisation. 1 hit.
PF00891. Methyltransf_2. 1 hit.
[Graphical view]
PIRSFPIRSF005739. O-mtase. 1 hit.
SUPFAMSSF53335. SSF53335. 1 hit.
PROSITEPS51683. SAM_OMT_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP28002.

Entry information

Entry nameCOMT1_MEDSA
AccessionPrimary (citable) accession number: P28002
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: July 9, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways