Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DNA-directed RNA polymerases I and III subunit RPAC2

Gene

RPC19

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerases catalyze the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common core component of RNA polymerases I and III which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs, respectively.3 Publications

GO - Molecular functioni

  • DNA binding Source: InterPro
  • RNA polymerase I activity Source: UniProtKB

GO - Biological processi

  • ribosome biogenesis Source: UniProtKB-KW
  • transcription from RNA polymerase I promoter Source: UniProtKB
  • tRNA transcription from RNA polymerase III promoter Source: SGD
Complete GO annotation...

Keywords - Biological processi

Ribosome biogenesis, Transcription

Enzyme and pathway databases

BioCyciYEAST:G3O-33137-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerases I and III subunit RPAC2
Short name:
RNA polymerases I and III subunit AC2
Alternative name(s):
AC19
DNA-directed RNA polymerases I and III 16 kDa polypeptide
RPA19
Gene namesi
Name:RPC19
Ordered Locus Names:YNL113W
ORF Names:N1937
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL113W.
SGDiS000005057. RPC19.

Subcellular locationi

GO - Cellular componenti

  • DNA-directed RNA polymerase I complex Source: UniProtKB
  • DNA-directed RNA polymerase III complex Source: SGD
  • nucleoplasm Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 142142DNA-directed RNA polymerases I and III subunit RPAC2PRO_0000149319Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei15 – 151PhosphothreonineCombined sources
Modified residuei33 – 331PhosphothreonineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP28000.
PeptideAtlasiP28000.

PTM databases

iPTMnetiP28000.

Interactioni

Subunit structurei

Component of the RNA polymerase I (Pol I) and RNA polymerase III (Pol III) complexes. Component of the RNA polymerase I (Pol I) complex consisting of 14 subunits: RPA135, RPA190, RPC40, RPA14, RPB5, RPO26, RPA43, RPB8, RPA12, RPB10, RPC19, RPC10, RPA49 and RPA34. The complex is composed of a horseshoe-shaped core containing ten subunits (RPA135, RPA190, RPB5, RPO26, RPB8, RPB10, RPC10, RPA12, RPC19 and RPC40) where RPA135 and RPA190 form the DNA-binding cleft. Outside of the core, RPA14 and RPA43 form the stalk that mediates interactions with transcription initiation factors and newly synthesized RNA. Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits. Directly interacts with the RPC40 subunit.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RPC40P077033EBI-15846,EBI-15831

Protein-protein interaction databases

BioGridi35712. 98 interactions.
DIPiDIP-130N.
IntActiP28000. 15 interactions.
MINTiMINT-391927.

Structurei

Secondary structure

1
142
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi46 – 494Combined sources
Turni51 – 533Combined sources
Beta strandi58 – 6710Combined sources
Helixi70 – 8011Combined sources
Beta strandi86 – 927Combined sources
Beta strandi96 – 983Combined sources
Beta strandi100 – 1078Combined sources
Beta strandi109 – 1113Combined sources
Helixi113 – 14129Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4C2MX-ray2.80K/Z1-142[»]
4C3HX-ray3.27K1-142[»]
4C3IX-ray3.0K1-142[»]
4C3JX-ray3.35K1-142[»]
4YM7X-ray5.50AK/BK/CK/DK/EK/FK1-142[»]
5FJ8electron microscopy3.90K1-142[»]
5FJ9electron microscopy4.60K1-142[»]
5FJAelectron microscopy4.65K1-142[»]
ProteinModelPortaliP28000.
SMRiP28000. Positions 42-142.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00550000075160.
HOGENOMiHOG000179862.
InParanoidiP28000.
KOiK03020.
OMAiRMDEVEF.
OrthoDBiEOG7T4MZH.

Family and domain databases

InterProiIPR009025. RBP11-like_dimer.
IPR008193. RNA_pol_Rpb11_13-16kDa_CS.
[Graphical view]
SUPFAMiSSF55257. SSF55257. 1 hit.
PROSITEiPS01154. RNA_POL_L_13KD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P28000-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEDIEQKKT ATEVTPQEPK HIQEEEEQDV DMTGDEEQEE EPDREKIKLL
60 70 80 90 100
TQATSEDGTS ASFQIVEEDH TLGNALRYVI MKNPDVEFCG YSIPHPSENL
110 120 130 140
LNIRIQTYGE TTAVDALQKG LKDLMDLCDV VESKFTEKIK SM
Length:142
Mass (Da):16,151
Last modified:August 1, 1992 - v1
Checksum:i0B048299A787EEDB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64991 Genomic DNA. Translation: AAA34998.1.
Z69382 Genomic DNA. Translation: CAA93394.1.
Z71390 Genomic DNA. Translation: CAA95994.1.
AY558001 Genomic DNA. Translation: AAS56327.1.
BK006947 Genomic DNA. Translation: DAA10434.1.
PIRiA39418.
RefSeqiNP_014286.1. NM_001182951.1.

Genome annotation databases

EnsemblFungiiYNL113W; YNL113W; YNL113W.
GeneIDi855609.
KEGGisce:YNL113W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64991 Genomic DNA. Translation: AAA34998.1.
Z69382 Genomic DNA. Translation: CAA93394.1.
Z71390 Genomic DNA. Translation: CAA95994.1.
AY558001 Genomic DNA. Translation: AAS56327.1.
BK006947 Genomic DNA. Translation: DAA10434.1.
PIRiA39418.
RefSeqiNP_014286.1. NM_001182951.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4C2MX-ray2.80K/Z1-142[»]
4C3HX-ray3.27K1-142[»]
4C3IX-ray3.0K1-142[»]
4C3JX-ray3.35K1-142[»]
4YM7X-ray5.50AK/BK/CK/DK/EK/FK1-142[»]
5FJ8electron microscopy3.90K1-142[»]
5FJ9electron microscopy4.60K1-142[»]
5FJAelectron microscopy4.65K1-142[»]
ProteinModelPortaliP28000.
SMRiP28000. Positions 42-142.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35712. 98 interactions.
DIPiDIP-130N.
IntActiP28000. 15 interactions.
MINTiMINT-391927.

PTM databases

iPTMnetiP28000.

Proteomic databases

MaxQBiP28000.
PeptideAtlasiP28000.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNL113W; YNL113W; YNL113W.
GeneIDi855609.
KEGGisce:YNL113W.

Organism-specific databases

EuPathDBiFungiDB:YNL113W.
SGDiS000005057. RPC19.

Phylogenomic databases

GeneTreeiENSGT00550000075160.
HOGENOMiHOG000179862.
InParanoidiP28000.
KOiK03020.
OMAiRMDEVEF.
OrthoDBiEOG7T4MZH.

Enzyme and pathway databases

BioCyciYEAST:G3O-33137-MONOMER.

Miscellaneous databases

NextBioi979786.
PROiP28000.

Family and domain databases

InterProiIPR009025. RBP11-like_dimer.
IPR008193. RNA_pol_Rpb11_13-16kDa_CS.
[Graphical view]
SUPFAMiSSF55257. SSF55257. 1 hit.
PROSITEiPS01154. RNA_POL_L_13KD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "RPC19, the gene for a subunit common to yeast RNA polymerases A (I) and C (III)."
    Dequard-Chablat M., Riva M., Carles C., Sentenac A.
    J. Biol. Chem. 266:15300-15307(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
  2. "The DNA sequence of cosmid 14-13b from chromosome XIV of Saccharomyces cerevisiae reveals an unusually high number of overlapping open reading frames."
    de Antoni A., D'Angelo M., Dal Pero F., Sartorello F., Pandolfo D., Pallavicini A., Lanfranchi G., Valle G.
    Yeast 13:261-266(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. "The yeast RNA polymerase III transcription machinery: a paradigm for eukaryotic gene activation."
    Chedin S., Ferri M.L., Peyroche G., Andrau J.-C., Jourdain S., Lefebvre O., Werner M., Carles C., Sentenac A.
    Cold Spring Harb. Symp. Quant. Biol. 63:381-389(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW ON THE RNA POL III COMPLEX.
  7. "Differential roles of phosphorylation in the formation of transcriptional active RNA polymerase I."
    Fath S., Milkereit P., Peyroche G., Riva M., Carles C., Tschochner H.
    Proc. Natl. Acad. Sci. U.S.A. 98:14334-14339(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE RNA POL I COMPLEX.
  8. "Interactions between three common subunits of yeast RNA polymerases I and III."
    Lalo D., Carles C., Sentenac A., Thuriaux P.
    Proc. Natl. Acad. Sci. U.S.A. 90:5524-5528(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH AC40.
  9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  11. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-33, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  12. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-15 AND THR-33, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-15 AND THR-33, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  14. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  15. "Structural biology of RNA polymerase III: subcomplex C17/25 X-ray structure and 11 subunit enzyme model."
    Jasiak A.J., Armache K.J., Martens B., Jansen R.P., Cramer P.
    Mol. Cell 23:71-81(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING OF THE POL III CORE COMPLEX.
  16. Cited for: STRUCTURE BY ELECTRON MICROSCOPY (12.00 ANGSTROMS) OF THE POL I COMPLEX, FUNCTION, SUBUNIT.
  17. Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF THE POL I COMPLEX, FUNCTION, SUBUNIT.
  18. "RNA polymerase I structure and transcription regulation."
    Engel C., Sainsbury S., Cheung A.C., Kostrewa D., Cramer P.
    Nature 502:650-655(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF THE POL I COMPLEX, FUNCTION, SUBUNIT.

Entry informationi

Entry nameiRPAC2_YEAST
AccessioniPrimary (citable) accession number: P28000
Secondary accession number(s): D6W168
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: May 11, 2016
This is version 156 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 8680 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.