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Protein

DNA-directed RNA polymerases I and III subunit RPAC2

Gene

RPC19

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerases catalyze the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common core component of RNA polymerases I and III which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs, respectively.3 Publications

Miscellaneous

Present with 8680 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • DNA binding Source: InterPro
  • protein dimerization activity Source: InterPro
  • RNA polymerase I activity Source: UniProtKB

GO - Biological processi

Keywordsi

Biological processRibosome biogenesis, Transcription

Enzyme and pathway databases

BioCyciYEAST:G3O-33137-MONOMER.
ReactomeiR-SCE-1834949. Cytosolic sensors of pathogen-associated DNA.
R-SCE-73762. RNA Polymerase I Transcription Initiation.
R-SCE-73780. RNA Polymerase III Chain Elongation.
R-SCE-73980. RNA Polymerase III Transcription Termination.
R-SCE-749476. RNA Polymerase III Abortive And Retractive Initiation.
R-SCE-76061. RNA Polymerase III Transcription Initiation From Type 1 Promoter.
R-SCE-76066. RNA Polymerase III Transcription Initiation From Type 2 Promoter.
R-SCE-76071. RNA Polymerase III Transcription Initiation From Type 3 Promoter.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerases I and III subunit RPAC2
Short name:
RNA polymerases I and III subunit AC2
Alternative name(s):
AC19
DNA-directed RNA polymerases I and III 16 kDa polypeptide
RPA19
Gene namesi
Name:RPC19
Ordered Locus Names:YNL113W
ORF Names:N1937
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL113W.
SGDiS000005057. RPC19.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001493191 – 142DNA-directed RNA polymerases I and III subunit RPAC2Add BLAST142

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei15PhosphothreonineCombined sources1
Modified residuei33PhosphothreonineCombined sources1
Cross-linki134Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP28000.
PRIDEiP28000.

PTM databases

iPTMnetiP28000.

Interactioni

Subunit structurei

Component of the RNA polymerase I (Pol I) and RNA polymerase III (Pol III) complexes. Component of the RNA polymerase I (Pol I) complex consisting of 14 subunits: RPA135, RPA190, RPC40, RPA14, RPB5, RPO26, RPA43, RPB8, RPA12, RPB10, RPC19, RPC10, RPA49 and RPA34. The complex is composed of a horseshoe-shaped core containing ten subunits (RPA135, RPA190, RPB5, RPO26, RPB8, RPB10, RPC10, RPA12, RPC19 and RPC40) where RPA135 and RPA190 form the DNA-binding cleft. Outside of the core, RPA14 and RPA43 form the stalk that mediates interactions with transcription initiation factors and newly synthesized RNA. Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits. Directly interacts with the RPC40 subunit.5 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi35712. 310 interactors.
DIPiDIP-130N.
IntActiP28000. 44 interactors.
MINTiMINT-391927.
STRINGi4932.YNL113W.

Structurei

Secondary structure

1142
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi46 – 49Combined sources4
Turni51 – 53Combined sources3
Beta strandi58 – 67Combined sources10
Helixi70 – 80Combined sources11
Beta strandi86 – 92Combined sources7
Beta strandi96 – 98Combined sources3
Beta strandi100 – 107Combined sources8
Beta strandi109 – 111Combined sources3
Helixi113 – 141Combined sources29

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4C2MX-ray2.80K/Z1-142[»]
4C3HX-ray3.27K1-142[»]
4C3IX-ray3.0K1-142[»]
4C3JX-ray3.35K1-142[»]
4YM7X-ray5.50AK/BK/CK/DK/EK/FK1-142[»]
5FJ8electron microscopy3.90K1-142[»]
5FJ9electron microscopy4.60K1-142[»]
5FJAelectron microscopy4.65K1-142[»]
5G5Lelectron microscopy4.80K1-142[»]
5LMXelectron microscopy4.90K1-142[»]
5M3Felectron microscopy3.80K1-142[»]
5M3Melectron microscopy4.00K1-142[»]
5M5Welectron microscopy3.80K1-142[»]
5M5Xelectron microscopy4.00K1-142[»]
5M5Yelectron microscopy4.00K1-142[»]
5M64electron microscopy4.60K1-142[»]
5N5Yelectron microscopy7.70K1-142[»]
5N5Zelectron microscopy7.70K1-142[»]
5N60electron microscopy7.70K1-142[»]
5N61electron microscopy3.40K1-142[»]
ProteinModelPortaliP28000.
SMRiP28000.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00550000075160.
HOGENOMiHOG000179862.
InParanoidiP28000.
KOiK03020.
OMAiSEPKMNI.
OrthoDBiEOG092C5QHE.

Family and domain databases

CDDicd07029. RNAP_I_III_AC19. 1 hit.
InterProiView protein in InterPro
IPR009025. RBP11-like_dimer.
IPR008193. RNA_pol_Rpb11_13-16kDa_CS.
IPR033898. RNAP_AC19.
PfamiView protein in Pfam
PF13656. RNA_pol_L_2. 1 hit.
SUPFAMiSSF55257. SSF55257. 1 hit.
PROSITEiView protein in PROSITE
PS01154. RNA_POL_L_13KD. 1 hit.

Sequencei

Sequence statusi: Complete.

P28000-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEDIEQKKT ATEVTPQEPK HIQEEEEQDV DMTGDEEQEE EPDREKIKLL
60 70 80 90 100
TQATSEDGTS ASFQIVEEDH TLGNALRYVI MKNPDVEFCG YSIPHPSENL
110 120 130 140
LNIRIQTYGE TTAVDALQKG LKDLMDLCDV VESKFTEKIK SM
Length:142
Mass (Da):16,151
Last modified:August 1, 1992 - v1
Checksum:i0B048299A787EEDB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64991 Genomic DNA. Translation: AAA34998.1.
Z69382 Genomic DNA. Translation: CAA93394.1.
Z71390 Genomic DNA. Translation: CAA95994.1.
AY558001 Genomic DNA. Translation: AAS56327.1.
BK006947 Genomic DNA. Translation: DAA10434.1.
PIRiA39418.
RefSeqiNP_014286.1. NM_001182951.1.

Genome annotation databases

EnsemblFungiiYNL113W; YNL113W; YNL113W.
GeneIDi855609.
KEGGisce:YNL113W.

Similar proteinsi

Entry informationi

Entry nameiRPAC2_YEAST
AccessioniPrimary (citable) accession number: P28000
Secondary accession number(s): D6W168
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: September 27, 2017
This is version 169 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names