ID   PALY_MEDSA              Reviewed;         725 AA.
AC   P27990;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   16-JUN-2009, entry version 60.
DE   RecName: Full=Phenylalanine ammonia-lyase;
DE            EC=4.3.1.24;
OS   Medicago sativa (Alfalfa).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids I; Fabales; Fabaceae; Papilionoideae; Trifolieae;
OC   Medicago.
OX   NCBI_TaxID=3879;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Apollo;
RX   MEDLINE=91355933; PubMed=1715786; DOI=10.1007/BF00040636;
RA   Gowri G., Paiva N.L., Dixon R.A.;
RT   "Stress responses in alfalfa (Medicago sativa L.) 12. Sequence
RT   analysis of phenylalanine ammonia-lyase (PAL) cDNA clones and
RT   appearance of PAL transcripts in elicitor-treated cell cultures and
RT   developing plants.";
RL   Plant Mol. Biol. 17:415-429(1991).
CC   -!- FUNCTION: This is a key enzyme of plant metabolism catalyzing the
CC       first reaction in the biosynthesis from L-phenylalanine of a wide
CC       variety of natural products based on the phenylpropane skeleton.
CC   -!- CATALYTIC ACTIVITY: L-phenylalanine = trans-cinnamate + ammonia.
CC   -!- PATHWAY: Phenylpropanoid metabolism; cinnamic acid biosynthesis;
CC       trans-cinnamic acid from L-phenylalanine: step 1/1.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (Probable).
CC   -!- PTM: Contains an active site 4-methylidene-imidazol-5-one (MIO),
CC       which is formed autocatalytically by cyclization and dehydration
CC       of residues Ala-Ser-Gly (By similarity).
CC   -!- SIMILARITY: Belongs to the PAL/histidase family.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; X58180; CAA41169.1; -; mRNA.
DR   PIR; S17444; S17444.
DR   HSSP; P21310; 1GKM.
DR   SMR; P27990; 34-725.
DR   BRENDA; 4.3.1.5; 815.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016211; F:ammonia ligase activity; IEA:InterPro.
DR   GO; GO:0016841; F:ammonia-lyase activity; IEA:InterPro.
DR   GO; GO:0009058; P:biosynthetic process; IEA:InterPro.
DR   GO; GO:0006559; P:L-phenylalanine catabolic process; IEA:InterPro.
DR   GO; GO:0009698; P:phenylpropanoid metabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR001106; Phe/His_NH3-lyase.
DR   InterPro; IPR005922; Phe_NH3-lyase.
DR   Pfam; PF00221; PAL; 1.
DR   TIGRFAMs; TIGR01226; phe_am_lyase; 1.
DR   PROSITE; PS00488; PAL_HISTIDASE; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Lyase; Phenylpropanoid metabolism.
FT   CHAIN         1    725       Phenylalanine ammonia-lyase.
FT                                /FTId=PRO_0000215400.
FT   COMPBIAS     24     27       Poly-Asn.
FT   MOD_RES     212    212       2,3-didehydroalanine (Ser) (By
FT                                similarity).
FT   CROSSLNK    211    213       5-imidazolinone (Ala-Gly) (By
FT                                similarity).
SQ   SEQUENCE   725 AA;  78866 MW;  19906A2575F64D7D CRC64;
     METISAAITK NNANESFCLI HAKNNNNMKV NEADPLNWGV AAEAMKGSHL DEVKRMVAEY
     RKPVVRLGGE TLTISQVAAI AAHDHGVQVD LSESARDGVK ASSEWVMESM NKGTDSYGVT
     TGFGATSHSR TKQGGALQKE LIRFLNAGIF GNGTESNHTL PKTATRAAML VRINTLLQGY
     SGIDFEILEA ITKPLNKTVT PCLPLRGTIT ASGDLVPLSY IAGLLTGRPN SKAHGPSGEV
     LNAKEAFNLA GINAEFFELQ PKEGLALVNG TAVGSGLASI VLFEANILAV LSEVLSAIFA
     EVMQGKPEFT DHLTHKLKHH PGQIEAAAIM EHILDGSSYV KAAKKLHEID PLQKPKQDRY
     ALRTSPQWLG PLVEVIRFST KSIEREINSV NDNPLIDVSR NKALHGGNFQ GTPIGVSMDN
     TRLALASIGK LMFAQFSELV NDFYNNGLPS NLSASRNPSL DYGFKGAEIA MASYCSELQY
     LANPVTTHVQ SAEQHNQDVN SLGLISARKT NEAIEILQLM SSTFLIALCQ AIDLRHLEEN
     LKNSVKNTVS QVAKKTLTMG VNGELHPSRF CEKDLLKVVD REHVFAYIDD PCSATYPLSQ
     KLRQVLVDHA LVNGESEKNF NTSIFQKIAT FEEELKTLLP KEVESARTAY ESGNPTIPNK
     INGCRSYPLY KFVREELGTG LLTGENVISP GEECDKLFSA MCQGKIIDPL LECLGEWNGA
     PLPIC
//