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Protein

Inositol-trisphosphate 3-kinase B

Gene

ITPKB

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + 1D-myo-inositol 1,4,5-trisphosphate = ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate.

Enzyme regulationi

IP3K is activated by calmodulin. Form B is much more sensitive to calcium/calmodulin than form A.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei679 – 6791ATPBy similarity
Binding sitei690 – 6901ATPBy similarity
Binding sitei743 – 7431ATPBy similarity
Binding sitei745 – 7451SubstrateBy similarity
Binding sitei766 – 7661SubstrateBy similarity
Binding sitei817 – 8171ATPBy similarity
Binding sitei897 – 8971ATPBy similarity
Binding sitei900 – 9001SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi730 – 7323ATPBy similarity

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • inositol-1,4,5-trisphosphate 3-kinase activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Calmodulin-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:HS07103-MONOMER.
BRENDAi2.7.1.127. 2681.
ReactomeiR-HSA-1855204. Synthesis of IP3 and IP4 in the cytosol.

Names & Taxonomyi

Protein namesi
Recommended name:
Inositol-trisphosphate 3-kinase B (EC:2.7.1.127)
Alternative name(s):
Inositol 1,4,5-trisphosphate 3-kinase B
Short name:
IP3 3-kinase B
Short name:
IP3K B
Short name:
InsP 3-kinase B
Gene namesi
Name:ITPKB
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:6179. ITPKB.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA29976.

Polymorphism and mutation databases

BioMutaiITPKB.
DMDMi62906885.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 946946Inositol-trisphosphate 3-kinase BPRO_0000066868Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei43 – 431PhosphoserineCombined sources
Modified residuei49 – 491PhosphoserineCombined sources
Modified residuei71 – 711PhosphoserineCombined sources
Modified residuei204 – 2041PhosphoserineBy similarity
Modified residuei269 – 2691PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP27987.
MaxQBiP27987.
PaxDbiP27987.
PeptideAtlasiP27987.
PRIDEiP27987.

PTM databases

iPTMnetiP27987.
PhosphoSiteiP27987.

Expressioni

Gene expression databases

BgeeiENSG00000143772.
CleanExiHS_ITPKB.
GenevisibleiP27987. HS.

Organism-specific databases

HPAiHPA030273.
HPA030275.

Interactioni

Subunit structurei

Interacts with DMTN.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
GOLGA2Q083794EBI-751388,EBI-618309

Protein-protein interaction databases

BioGridi109912. 8 interactions.
IntActiP27987. 7 interactions.
MINTiMINT-1454586.
STRINGi9606.ENSP00000272117.

Structurei

3D structure databases

ProteinModelPortaliP27987.
SMRiP27987. Positions 654-941.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni768 – 7769Calmodulin-bindingBy similarity
Regioni793 – 8008Substrate bindingBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi599 – 6057Poly-Ser

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1621. Eukaryota.
ENOG410Y8AC. LUCA.
GeneTreeiENSGT00390000017438.
HOGENOMiHOG000060305.
HOVERGENiHBG052139.
InParanoidiP27987.
KOiK00911.
OMAiQGQFLGS.
OrthoDBiEOG091G03J3.
PhylomeDBiP27987.
TreeFamiTF318394.

Family and domain databases

InterProiIPR005522. IPK.
[Graphical view]
PANTHERiPTHR12400. PTHR12400. 2 hits.
PfamiPF03770. IPK. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P27987-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAVYCYALNS LVIMNSANEM KSGGGPGPSG SETPPPPRRA VLSPGSVFSP
60 70 80 90 100
GRGASFLFPP AESLSPEEPR SPGGWRSGRR RLNSSSGSGS GSSGSSVSSP
110 120 130 140 150
SWAGRLRGDR QQVVAAGTLS PPGPEEAKRK LRILQRELQN VQVNQKVGMF
160 170 180 190 200
EAHIQAQSSA IQAPRSPRLG RARSPSPCPF RSSSQPPGRV LVQGARSEER
210 220 230 240 250
RTKSWGEQCP ETSGTDSGRK GGPSLCSSQV KKGMPPLPGR AAPTGSEAQG
260 270 280 290 300
PSAFVRMEKG IPASPRCGSP TAMEIDKRGS PTPGTRSCLA PSLGLFGASL
310 320 330 340 350
TMATEVAARV TSTGPHRPQD LALTEPSGRA RELEDLQPPE ALVERQGQFL
360 370 380 390 400
GSETSPAPER GGPRDGEPPG KMGKGYLPCG MPGSGEPEVG KRPEETTVSV
410 420 430 440 450
QSAESSDSLS WSRLPRALAS VGPEEARSGA PVGGGRWQLS DRVEGGSPTL
460 470 480 490 500
GLLGGSPSAQ PGTGNVEAGI PSGRMLEPLP CWDAAKDLKE PQCPPGDRVG
510 520 530 540 550
VQPGNSRVWQ GTMEKAGLAW TRGTGVQSEG TWESQRQDSD ALPSPELLPQ
560 570 580 590 600
DPDKPFLRKA CSPSNIPAVI ITDMGTQEDG ALEETQGSPR GNLPLRKLSS
610 620 630 640 650
SSASSTGFSS SYEDSEEDIS SDPERTLDPN SAFLHTLDQQ KPRVSKSWRK
660 670 680 690 700
IKNMVHWSPF VMSFKKKYPW IQLAGHAGSF KAAANGRILK KHCESEQRCL
710 720 730 740 750
DRLMVDVLRP FVPAYHGDVV KDGERYNQMD DLLADFDSPC VMDCKMGIRT
760 770 780 790 800
YLEEELTKAR KKPSLRKDMY QKMIEVDPEA PTEEEKAQRA VTKPRYMQWR
810 820 830 840 850
ETISSTATLG FRIEGIKKED GTVNRDFKKT KTREQVTEAF REFTKGNHNI
860 870 880 890 900
LIAYRDRLKA IRTTLEVSPF FKCHEVIGSS LLFIHDKKEQ AKVWMIDFGK
910 920 930 940
TTPLPEGQTL QHDVPWQEGN REDGYLSGLN NLVDILTEMS QDAPLA
Length:946
Mass (Da):102,376
Last modified:April 26, 2005 - v5
Checksum:i2CCBCBE7AEF26848
GO
Isoform 2 (identifier: P27987-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     645-946: Missing.

Note: No experimental confirmation available.
Show »
Length:644
Mass (Da):67,387
Checksum:iDDD95CE2574116EC
GO

Sequence cautioni

The sequence CAA40491 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti173 – 1731R → H in CAC40650 (Ref. 2) Curated
Sequence conflicti173 – 1731R → H in AAH15009 (PubMed:15489334).Curated
Sequence conflicti210 – 2101P → S in CAC40650 (Ref. 2) Curated
Sequence conflicti297 – 3015GASLT → APSFP in CAC40650 (Ref. 2) Curated
Sequence conflicti442 – 4432RV → IP (PubMed:1654894).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti322 – 3221A → T.
Corresponds to variant rs3754413 [ dbSNP | Ensembl ].
VAR_053444
Natural varianti408 – 4081S → A.2 Publications
Corresponds to variant rs6667260 [ dbSNP | Ensembl ].
VAR_023768
Natural varianti552 – 5521P → Q.4 Publications
Corresponds to variant rs708776 [ dbSNP | Ensembl ].
VAR_022380

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei645 – 946302Missing in isoform 2. 1 PublicationVSP_016092Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y18024 mRNA. Translation: CAB65055.3.
AJ242780 mRNA. Translation: CAC40650.1.
AL365444 Genomic DNA. Translation: CAH70187.1.
AL365444 Genomic DNA. Translation: CAH70188.1.
BC015009 mRNA. Translation: AAH15009.1.
X57206 mRNA. Translation: CAA40491.1. Different initiation.
CCDSiCCDS1555.1. [P27987-1]
PIRiJC7810.
S17682.
RefSeqiNP_002212.3. NM_002221.3. [P27987-1]
XP_005273177.1. XM_005273120.2. [P27987-2]
UniGeneiHs.528087.
Hs.659396.

Genome annotation databases

EnsembliENST00000272117; ENSP00000272117; ENSG00000143772. [P27987-1]
ENST00000366784; ENSP00000355748; ENSG00000143772. [P27987-2]
ENST00000429204; ENSP00000411152; ENSG00000143772. [P27987-1]
GeneIDi3707.
KEGGihsa:3707.
UCSCiuc001hqh.4. human. [P27987-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y18024 mRNA. Translation: CAB65055.3.
AJ242780 mRNA. Translation: CAC40650.1.
AL365444 Genomic DNA. Translation: CAH70187.1.
AL365444 Genomic DNA. Translation: CAH70188.1.
BC015009 mRNA. Translation: AAH15009.1.
X57206 mRNA. Translation: CAA40491.1. Different initiation.
CCDSiCCDS1555.1. [P27987-1]
PIRiJC7810.
S17682.
RefSeqiNP_002212.3. NM_002221.3. [P27987-1]
XP_005273177.1. XM_005273120.2. [P27987-2]
UniGeneiHs.528087.
Hs.659396.

3D structure databases

ProteinModelPortaliP27987.
SMRiP27987. Positions 654-941.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109912. 8 interactions.
IntActiP27987. 7 interactions.
MINTiMINT-1454586.
STRINGi9606.ENSP00000272117.

PTM databases

iPTMnetiP27987.
PhosphoSiteiP27987.

Polymorphism and mutation databases

BioMutaiITPKB.
DMDMi62906885.

Proteomic databases

EPDiP27987.
MaxQBiP27987.
PaxDbiP27987.
PeptideAtlasiP27987.
PRIDEiP27987.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000272117; ENSP00000272117; ENSG00000143772. [P27987-1]
ENST00000366784; ENSP00000355748; ENSG00000143772. [P27987-2]
ENST00000429204; ENSP00000411152; ENSG00000143772. [P27987-1]
GeneIDi3707.
KEGGihsa:3707.
UCSCiuc001hqh.4. human. [P27987-1]

Organism-specific databases

CTDi3707.
GeneCardsiITPKB.
H-InvDBHIX0001645.
HGNCiHGNC:6179. ITPKB.
HPAiHPA030273.
HPA030275.
MIMi147522. gene.
neXtProtiNX_P27987.
PharmGKBiPA29976.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1621. Eukaryota.
ENOG410Y8AC. LUCA.
GeneTreeiENSGT00390000017438.
HOGENOMiHOG000060305.
HOVERGENiHBG052139.
InParanoidiP27987.
KOiK00911.
OMAiQGQFLGS.
OrthoDBiEOG091G03J3.
PhylomeDBiP27987.
TreeFamiTF318394.

Enzyme and pathway databases

BioCyciMetaCyc:HS07103-MONOMER.
BRENDAi2.7.1.127. 2681.
ReactomeiR-HSA-1855204. Synthesis of IP3 and IP4 in the cytosol.

Miscellaneous databases

ChiTaRSiITPKB. human.
GeneWikiiITPKB.
GenomeRNAii3707.
PROiP27987.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000143772.
CleanExiHS_ITPKB.
GenevisibleiP27987. HS.

Family and domain databases

InterProiIPR005522. IPK.
[Graphical view]
PANTHERiPTHR12400. PTHR12400. 2 hits.
PfamiPF03770. IPK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIP3KB_HUMAN
AccessioniPrimary (citable) accession number: P27987
Secondary accession number(s): Q5VWL9
, Q5VWM0, Q96BZ2, Q96JS1, Q9UH47
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: April 26, 2005
Last modified: September 7, 2016
This is version 151 of the entry and version 5 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.