Reviewed, UniProtKB/Swiss-Prot P27987 (IP3KB_HUMAN)

Names and origin

Protein names

Recommended name:
    Inositol-trisphosphate 3-kinase B
    EC=2.7.1.127
Alternative name(s):
    Inositol 1,4,5-trisphosphate 3-kinase B
    IP3 3-kinase B
      Short name=IP3K-B
      Short name=IP3K B

Gene names

Name:

ITPKB

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	946 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + 1D-myo-inositol 1,4,5-trisphosphate = ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate.
Enzyme regulation	IP3K is activated by calmodulin. Form B is much more sensitive to calcium/calmodulin than form A.
Sequence similarities	Belongs to the inositol phosphokinase (IPK) family.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	ATP-binding Calmodulin-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	signal transduction Ref.5 Traceable author statement. Source: ProtInc
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW calmodulin binding Inferred from electronic annotation. Source: UniProtKB-KW inositol trisphosphate 3-kinase activity Ref.5 Traceable author statement. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P27987-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P27987-2)

The sequence of this isoform differs from the canonical sequence as follows:
     645-946: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 946	946	Inositol-trisphosphate 3-kinase B		PRO_0000066868
Regions
	Nucleotide binding	730 – 732	3	ATP By similarity
	Region	768 – 776	9	Calmodulin-binding By similarity
	Region	793 – 800	8	Substrate binding By similarity
	Compositional bias	599 – 605	7	Poly-Ser
Sites
	Binding site	679	1	ATP By similarity
	Binding site	690	1	ATP By similarity
	Binding site	743	1	ATP By similarity
	Binding site	745	1	Substrate By similarity
	Binding site	766	1	Substrate By similarity
	Binding site	817	1	ATP By similarity
	Binding site	897	1	ATP By similarity
	Binding site	900	1	Substrate By similarity
Amino acid modifications
	Modified residue	43	1	Phosphoserine Ref.6 Ref.7
	Modified residue	49	1	Phosphoserine Ref.6 Ref.7
	Modified residue	65	1	Phosphoserine Ref.7
	Modified residue	71	1	Phosphoserine Ref.7
	Modified residue	174	1	Phosphoserine Ref.7
	Modified residue	176	1	Phosphoserine Ref.7
Natural variations
	Alternative sequence	645 – 946	302	Missing in isoform 2.		VSP_016092
	Natural variant	322	1	A → T: dbSNP rs3754413.		VAR_053444
	Natural variant	408	1	S → A: dbSNP rs6667260. Ref.1 Ref.4		VAR_023768
	Natural variant	552	1	P → Q: dbSNP rs708776. Ref.1 Ref.4 Ref.2 Ref.5		VAR_022380
Experimental info
	Sequence conflict	173	1	R → H in CAC40650. Ref.2
	Sequence conflict	173	1	R → H in AAH15009. Ref.4
	Sequence conflict	210	1	P → S in CAC40650. Ref.2
	Sequence conflict	297 – 301	5	GASLT → APSFP in CAC40650. Ref.2
	Sequence conflict	442 – 443	2	RV → IP Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 26, 2005. Version 5. Checksum: 2CCBCBE7AEF26848	FASTA	946	102,376
	10 20 30 40 50 60 MAVYCYALNS LVIMNSANEM KSGGGPGPSG SETPPPPRRA VLSPGSVFSP GRGASFLFPP 70 80 90 100 110 120 AESLSPEEPR SPGGWRSGRR RLNSSSGSGS GSSGSSVSSP SWAGRLRGDR QQVVAAGTLS 130 140 150 160 170 180 PPGPEEAKRK LRILQRELQN VQVNQKVGMF EAHIQAQSSA IQAPRSPRLG RARSPSPCPF 190 200 210 220 230 240 RSSSQPPGRV LVQGARSEER RTKSWGEQCP ETSGTDSGRK GGPSLCSSQV KKGMPPLPGR 250 260 270 280 290 300 AAPTGSEAQG PSAFVRMEKG IPASPRCGSP TAMEIDKRGS PTPGTRSCLA PSLGLFGASL 310 320 330 340 350 360 TMATEVAARV TSTGPHRPQD LALTEPSGRA RELEDLQPPE ALVERQGQFL GSETSPAPER 370 380 390 400 410 420 GGPRDGEPPG KMGKGYLPCG MPGSGEPEVG KRPEETTVSV QSAESSDSLS WSRLPRALAS 430 440 450 460 470 480 VGPEEARSGA PVGGGRWQLS DRVEGGSPTL GLLGGSPSAQ PGTGNVEAGI PSGRMLEPLP 490 500 510 520 530 540 CWDAAKDLKE PQCPPGDRVG VQPGNSRVWQ GTMEKAGLAW TRGTGVQSEG TWESQRQDSD 550 560 570 580 590 600 ALPSPELLPQ DPDKPFLRKA CSPSNIPAVI ITDMGTQEDG ALEETQGSPR GNLPLRKLSS 610 620 630 640 650 660 SSASSTGFSS SYEDSEEDIS SDPERTLDPN SAFLHTLDQQ KPRVSKSWRK IKNMVHWSPF 670 680 690 700 710 720 VMSFKKKYPW IQLAGHAGSF KAAANGRILK KHCESEQRCL DRLMVDVLRP FVPAYHGDVV 730 740 750 760 770 780 KDGERYNQMD DLLADFDSPC VMDCKMGIRT YLEEELTKAR KKPSLRKDMY QKMIEVDPEA 790 800 810 820 830 840 PTEEEKAQRA VTKPRYMQWR ETISSTATLG FRIEGIKKED GTVNRDFKKT KTREQVTEAF 850 860 870 880 890 900 REFTKGNHNI LIAYRDRLKA IRTTLEVSPF FKCHEVIGSS LLFIHDKKEQ AKVWMIDFGK 910 920 930 940 TTPLPEGQTL QHDVPWQEGN REDGYLSGLN NLVDILTEMS QDAPLA
	Isoform 2. Checksum: DDD95CE2574116EC	FASTA	644	67,387
	10 20 30 40 50 60 MAVYCYALNS LVIMNSANEM KSGGGPGPSG SETPPPPRRA VLSPGSVFSP GRGASFLFPP 70 80 90 100 110 120 AESLSPEEPR SPGGWRSGRR RLNSSSGSGS GSSGSSVSSP SWAGRLRGDR QQVVAAGTLS 130 140 150 160 170 180 PPGPEEAKRK LRILQRELQN VQVNQKVGMF EAHIQAQSSA IQAPRSPRLG RARSPSPCPF 190 200 210 220 230 240 RSSSQPPGRV LVQGARSEER RTKSWGEQCP ETSGTDSGRK GGPSLCSSQV KKGMPPLPGR 250 260 270 280 290 300 AAPTGSEAQG PSAFVRMEKG IPASPRCGSP TAMEIDKRGS PTPGTRSCLA PSLGLFGASL 310 320 330 340 350 360 TMATEVAARV TSTGPHRPQD LALTEPSGRA RELEDLQPPE ALVERQGQFL GSETSPAPER 370 380 390 400 410 420 GGPRDGEPPG KMGKGYLPCG MPGSGEPEVG KRPEETTVSV QSAESSDSLS WSRLPRALAS 430 440 450 460 470 480 VGPEEARSGA PVGGGRWQLS DRVEGGSPTL GLLGGSPSAQ PGTGNVEAGI PSGRMLEPLP 490 500 510 520 530 540 CWDAAKDLKE PQCPPGDRVG VQPGNSRVWQ GTMEKAGLAW TRGTGVQSEG TWESQRQDSD 550 560 570 580 590 600 ALPSPELLPQ DPDKPFLRKA CSPSNIPAVI ITDMGTQEDG ALEETQGSPR GNLPLRKLSS 610 620 630 640 SSASSTGFSS SYEDSEEDIS SDPERTLDPN SAFLHTLDQQ KPRV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression of a full-length cDNA encoding human inositol 1,4,5-trisphosphate 3-kinase B." Dewaste V., Roymans D., Moreau C., Erneux C. Biochem. Biophys. Res. Commun. 291:400-405(2002) [PubMed: 11846419] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANTS ALA-408 AND GLN-552. Tissue: Hippocampus.
[2]	"Cloning of the complete protein coding regions for inositol 1,4,5-trisphosphate 3-kinase B-isoforms from rat and human." Bertsch U., Suesse S., Frerk S., Fanick W. Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT GLN-552. Tissue: Colon.
[3]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANTS ALA-408 AND GLN-552. Tissue: Skin.
[5]	"Molecular cloning and expression of a new putative inositol 1,4,5-trisphosphate 3-kinase isoenzyme." Takazawa K., Perret J., Dumont J.E., Erneux C. Biochem. J. 278:883-886(1991) [PubMed: 1654894] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 442-946 (ISOFORM 1), VARIANT GLN-552. Tissue: Hippocampus.
[6]	"Phosphoproteome of resting human platelets." Zahedi R.P., Lewandrowski U., Wiesner J., Wortelkamp S., Moebius J., Schuetz C., Walter U., Gambaryan S., Sickmann A. J. Proteome Res. 7:526-534(2008) [PubMed: 18088087] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-43 AND SER-49, MASS SPECTROMETRY. Tissue: Platelet.
[7]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-43; SER-49; SER-65; SER-71; SER-174 AND SER-176, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y18024 mRNA. Translation: CAB65055.3. AJ242780 mRNA. Translation: CAC40650.1. AL365444 Genomic DNA. Translation: CAH70187.1. AL365444 Genomic DNA. Translation: CAH70188.1. BC015009 mRNA. Translation: AAH15009.1. X57206 mRNA. Translation: CAA40491.1. Different initiation.
IPI	IPI00021449. IPI00645189.
PIR	JC7810. S17682.
RefSeq	NP_002212.3.
UniGene	Hs.528087 Hs.659396
3D structure databases
SMR	P27987. Positions 654-941.
ModBase	Search...
Protein-protein interaction databases
IntAct	P27987. 1 interaction.
STRING	P27987.
PTM databases
PhosphoSite	P27987.
Proteomic databases
PRIDE	P27987.
Genome annotation databases
Ensembl	ENST00000272117; ENSP00000272117; ENSG00000143772; Homo sapiens. [Genome view] ENST00000366784; ENSP00000355748; ENSG00000143772; Homo sapiens. [Genome view] ENST00000429204; ENSP00000411152; ENSG00000143772; Homo sapiens. [Genome view]
GeneID	3707.
KEGG	hsa:3707.
UCSC	uc001hqg.1. human. uc001hqh.2. human.
Organism-specific databases
CTD	3707.
GeneCards	GC01M224886.
HGNC	HGNC:6179. ITPKB.
MIM	147522. gene.
PharmGKB	PA29976.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	P27987.
HOVERGEN	P27987.
OMA	PSWAGRL.
Enzyme and pathway databases
BRENDA	2.7.1.127. 247.
Gene expression databases
ArrayExpress	P27987.
Bgee	P27987.
CleanEx	HS_ITPKB.
Genevestigator	P27987.
GermOnline	ENSG00000143772. Homo sapiens.
Family and domain databases
InterPro	IPR005522. IPK. [Graphical view]
PANTHER	PTHR12400. IPK. 1 hit.
Pfam	PF03770. IPK. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	14529.
SOURCE	Search...

Entry information

Entry name

IP3KB_HUMAN

Accession

Primary (citable) accession number: P27987
Secondary accession number(s): Q5VWL9 , Q5VWM0, Q96BZ2, Q96JS1, Q9UH47

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	April 26, 2005
Last modified:	November 3, 2009
This is version 83 of the entry and version 5 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.