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Protein

Nitrate reductase [NAD(P)H]

Gene

NAR-7

Organism
Hordeum vulgare (Barley)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.

Catalytic activityi

Nitrite + NAD(P)+ + H2O = nitrate + NAD(P)H.

Cofactori

Protein has several cofactor binding sites:
  • FADBy similarityNote: Binds 1 FAD per subunit.By similarity
  • hemeBy similarityNote: Binds 1 heme group per subunit.By similarity
  • Mo-molybdopterinBy similarityNote: Binds 1 Mo-molybdopterin (Mo-MPT) cofactor per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi168MolybdenumBy similarity1
Metal bindingi550Iron (heme axial ligand)PROSITE-ProRule annotation1
Metal bindingi573Iron (heme axial ligand)PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Nitrate assimilation

Keywords - Ligandi

FAD, Flavoprotein, Heme, Iron, Metal-binding, Molybdenum, NAD, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrate reductase [NAD(P)H] (EC:1.7.1.2)
Gene namesi
Name:NAR-7
OrganismiHordeum vulgare (Barley)
Taxonomic identifieri4513 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladePooideaeTriticodaeTriticeaeHordeinaeHordeum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001660581 – 891Nitrate reductase [NAD(P)H]Add BLAST891

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi406InterchainSequence analysis

Keywords - PTMi

Disulfide bond

Expressioni

Inductioni

By nitrate.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

IntActiP27968. 1 interactor.
STRINGi4513.MLOC_5716.1.

Structurei

3D structure databases

ProteinModelPortaliP27968.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini515 – 590Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd BLAST76
Domaini630 – 742FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST113

Sequence similaritiesi

Belongs to the nitrate reductase family.Curated
Contains 1 cytochrome b5 heme-binding domain.PROSITE-ProRule annotation
Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0534. Eukaryota.
KOG0535. Eukaryota.
KOG0537. Eukaryota.
COG0543. LUCA.
COG2041. LUCA.
COG5274. LUCA.

Family and domain databases

Gene3Di2.60.40.650. 1 hit.
3.10.120.10. 1 hit.
3.90.420.10. 1 hit.
InterProiIPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR014756. Ig_E-set.
IPR005066. MoCF_OxRdtse_dimer.
IPR008335. Mopterin_OxRdtase_euk.
IPR001834. NADH-Cyt_B5_reductase.
IPR012137. Nitr_rd_NADH.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR000572. OxRdtase_Mopterin-bd_dom.
IPR022407. OxRdtase_Mopterin_BS.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
PF00970. FAD_binding_6. 1 hit.
PF03404. Mo-co_dimer. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF00174. Oxidored_molyb. 1 hit.
[Graphical view]
PIRSFiPIRSF000233. Nitr_rd_NADH. 1 hit.
PRINTSiPR00406. CYTB5RDTASE.
PR00363. CYTOCHROMEB5.
PR00407. EUMOPTERIN.
PR00371. FPNCR.
SMARTiSM01117. Cyt-b5. 1 hit.
[Graphical view]
SUPFAMiSSF55856. SSF55856. 1 hit.
SSF56524. SSF56524. 1 hit.
SSF63380. SSF63380. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
PS51384. FAD_FR. 1 hit.
PS00559. MOLYBDOPTERIN_EUK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P27968-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAASVEYNRQ VSAHPWPTNA QPKAAFDLFS SSGGGRRRSG ADSDSDDEDS
60 70 80 90 100
VPPDWRSLYS PRLDVEPSVK DPRDEATSDA WVKRHPALVR LTGKHPFNSE
110 120 130 140 150
PPLPRLMSHG FITPVPLHYV RNHGAVPKAD WSTWTVEVTG LVKRPVKFTM
160 170 180 190 200
EELVTGFQAV EFPVTLVCAG NRRKEQNMVR QSSGFNWGPG AISTTVWRGV
210 220 230 240 250
RLRDVLRRCG VMGAGAASNV CFEGAEDLPG GGGCKYGTSL RRSVAMDPAR
260 270 280 290 300
DVILAYMQNG EPLAPDHGFP VRVIVPGFIG GRMVKWLKRI VVACNESESY
310 320 330 340 350
YHYRDNRVLP SHVDAELANA EAWWYKPECM INELNINSVI TTPGHDEVLP
360 370 380 390 400
INALTTQKPY TMKGYAYSGG GRKVTRVEVT LDGGETWQVC DLEHPERPTK
410 420 430 440 450
YGKYWCWCFW SVEVEVLELL GAKEMAVRAW DEALNTQPER LIWNLMGMMN
460 470 480 490 500
NCWFRVKINV CRPHKGEIGL VFDHPTQPGN QSGGWMARQK HIETSETTQG
510 520 530 540 550
TLKRSTSTPF MSTASAQFTM SEVRRHASKD SAWIVVHGHV YDCTAFLKDH
560 570 580 590 600
PGGADSILIN AGSDCTEEFD AIHSAKARGL LEMYRVGELI VTGNDYSPQS
610 620 630 640 650
SNADLAAIVE APAVVVPRLP ASAVALANPR EKVRCRLVDK KSMSHNVRLF
660 670 680 690 700
RFALPSPDQK LGLPVGKHVY VCASTGGKLC MRAYTPTSSV EEVGHVELLI
710 720 730 740 750
KIYFKDEDPK FPAGGLMSQY LDALPLGAPV DIKGPVGHIE YAGRGAFTVG
760 770 780 790 800
GERRFARRLA MVAGGTGITP VYQVIQAVLR DQPDDTTEMH LVYANRTEDD
810 820 830 840 850
MLLREEIDRW AAANPARLKV WYVVSKVGRP EDAWEYGVGR VDEQVLREHL
860 870 880 890
PLGGDGETLA LVCGPPAMLE CTVRPGLEKM GYDLDKDCLV F
Length:891
Mass (Da):98,630
Last modified:August 1, 1992 - v1
Checksum:iAA47EC52FC1EFD13
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60173 Genomic DNA. Translation: CAA42739.1.
PIRiS16895. RDBHNP.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60173 Genomic DNA. Translation: CAA42739.1.
PIRiS16895. RDBHNP.

3D structure databases

ProteinModelPortaliP27968.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP27968. 1 interactor.
STRINGi4513.MLOC_5716.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiKOG0534. Eukaryota.
KOG0535. Eukaryota.
KOG0537. Eukaryota.
COG0543. LUCA.
COG2041. LUCA.
COG5274. LUCA.

Family and domain databases

Gene3Di2.60.40.650. 1 hit.
3.10.120.10. 1 hit.
3.90.420.10. 1 hit.
InterProiIPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR014756. Ig_E-set.
IPR005066. MoCF_OxRdtse_dimer.
IPR008335. Mopterin_OxRdtase_euk.
IPR001834. NADH-Cyt_B5_reductase.
IPR012137. Nitr_rd_NADH.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR000572. OxRdtase_Mopterin-bd_dom.
IPR022407. OxRdtase_Mopterin_BS.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
PF00970. FAD_binding_6. 1 hit.
PF03404. Mo-co_dimer. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF00174. Oxidored_molyb. 1 hit.
[Graphical view]
PIRSFiPIRSF000233. Nitr_rd_NADH. 1 hit.
PRINTSiPR00406. CYTB5RDTASE.
PR00363. CYTOCHROMEB5.
PR00407. EUMOPTERIN.
PR00371. FPNCR.
SMARTiSM01117. Cyt-b5. 1 hit.
[Graphical view]
SUPFAMiSSF55856. SSF55856. 1 hit.
SSF56524. SSF56524. 1 hit.
SSF63380. SSF63380. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
PS51384. FAD_FR. 1 hit.
PS00559. MOLYBDOPTERIN_EUK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNIA7_HORVU
AccessioniPrimary (citable) accession number: P27968
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: October 5, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.