Reviewed, UniProtKB/Swiss-Prot P27968 (NIA7_HORVU)

Names and origin

Protein names

Recommended name:
    Nitrate reductase [NAD(P)H]
    EC=1.7.1.2

Gene names

Name:

NAR-7

Organism

Hordeum vulgare (Barley)

Taxonomic identifier

4513 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Hordeum

Protein attributes

Sequence length	891 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.
Catalytic activity	Nitrite + NAD(P)+ + H2O = nitrate + NAD(P)H.
Cofactor	Binds 1 FAD per subunit. Binds 1 heme group per subunit. Binds 1 molybdenum-pterin group per subunit.
Subunit structure	Homodimer.
Induction	By nitrate.
Sequence similarities	Belongs to the nitrate reductase family. Contains 1 cytochrome b5 heme-binding domain. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Biological process	Nitrate assimilation
Ligand	FAD Flavoprotein Heme Iron Metal-binding Molybdenum NAD NADP
Molecular function	Oxidoreductase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	nitrate assimilation Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro molybdenum ion binding Inferred from electronic annotation. Source: UniProtKB-KW nitrate reductase [NAD(P)H] activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 891	891	Nitrate reductase [NAD(P)H]		PRO_0000166058
Regions
	Domain	515 – 590	76	Cytochrome b5 heme-binding
	Domain	630 – 742	113	FAD-binding FR-type
Sites
	Metal binding	168	1	Molybdenum-pterin Potential
	Metal binding	221	1	Molybdenum-pterin Potential
	Metal binding	550	1	Iron (heme axial ligand) By similarity
	Metal binding	573	1	Iron (heme axial ligand) By similarity
Amino acid modifications
	Disulfide bond	406		Interchain Potential

Sequences

	Sequence		Length	Mass (Da)
	P27968-1 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: AA47EC52FC1EFD13	FASTA	891	98,630
	10 20 30 40 50 60 MAASVEYNRQ VSAHPWPTNA QPKAAFDLFS SSGGGRRRSG ADSDSDDEDS VPPDWRSLYS 70 80 90 100 110 120 PRLDVEPSVK DPRDEATSDA WVKRHPALVR LTGKHPFNSE PPLPRLMSHG FITPVPLHYV 130 140 150 160 170 180 RNHGAVPKAD WSTWTVEVTG LVKRPVKFTM EELVTGFQAV EFPVTLVCAG NRRKEQNMVR 190 200 210 220 230 240 QSSGFNWGPG AISTTVWRGV RLRDVLRRCG VMGAGAASNV CFEGAEDLPG GGGCKYGTSL 250 260 270 280 290 300 RRSVAMDPAR DVILAYMQNG EPLAPDHGFP VRVIVPGFIG GRMVKWLKRI VVACNESESY 310 320 330 340 350 360 YHYRDNRVLP SHVDAELANA EAWWYKPECM INELNINSVI TTPGHDEVLP INALTTQKPY 370 380 390 400 410 420 TMKGYAYSGG GRKVTRVEVT LDGGETWQVC DLEHPERPTK YGKYWCWCFW SVEVEVLELL 430 440 450 460 470 480 GAKEMAVRAW DEALNTQPER LIWNLMGMMN NCWFRVKINV CRPHKGEIGL VFDHPTQPGN 490 500 510 520 530 540 QSGGWMARQK HIETSETTQG TLKRSTSTPF MSTASAQFTM SEVRRHASKD SAWIVVHGHV 550 560 570 580 590 600 YDCTAFLKDH PGGADSILIN AGSDCTEEFD AIHSAKARGL LEMYRVGELI VTGNDYSPQS 610 620 630 640 650 660 SNADLAAIVE APAVVVPRLP ASAVALANPR EKVRCRLVDK KSMSHNVRLF RFALPSPDQK 670 680 690 700 710 720 LGLPVGKHVY VCASTGGKLC MRAYTPTSSV EEVGHVELLI KIYFKDEDPK FPAGGLMSQY 730 740 750 760 770 780 LDALPLGAPV DIKGPVGHIE YAGRGAFTVG GERRFARRLA MVAGGTGITP VYQVIQAVLR 790 800 810 820 830 840 DQPDDTTEMH LVYANRTEDD MLLREEIDRW AAANPARLKV WYVVSKVGRP EDAWEYGVGR 850 860 870 880 890 VDEQVLREHL PLGGDGETLA LVCGPPAMLE CTVRPGLEKM GYDLDKDCLV F

References

[1]	"Characterization and sequence of a novel nitrate reductase from barley." Miyazaki J., Juricek M., Angelis K., Schnorr K.M., Kleinhofs A., Warner R.L. Mol. Gen. Genet. 228:329-334(1991) [PubMed: 1896007] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Himalaya.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X60173 Genomic DNA. Translation: CAA42739.1.
PIR	RDBHNP. S16895.
3D structure databases
HSSP	HSSP built from PDB template 2CND based on UniProtKB P17571.
ModBase	Search...
Organism-specific databases
Gramene	P27968.
Enzyme and pathway databases
BRENDA	1.7.1.2. 283.
Family and domain databases
InterPro	IPR001199. Cyt_B5. IPR018506. Cyt_B5_heme-BS. IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR005066. MoCF_OxRdtse_dimer. IPR008335. Mopterin_OxRdtase_euk. IPR001834. NADH-Cyt_B5_reductase. IPR012137. Nitr_rd_NADH. IPR008333. OxRdtase_FAD-bd. IPR001433. OxRdtase_FAD/NAD_bd. IPR000572. OxRdtase_Mopterin-bd. [Graphical view]
Gene3D	G3DSA:3.10.120.10. Cyt_B5. 1 hit. G3DSA:2.60.40.650. MoCF_oxrdtse_dimer. 1 hit. G3DSA:3.90.420.10. Oxred_molyb_bd. 1 hit.
Pfam	PF00173. Cyt-b5. 1 hit. PF00970. FAD_binding_6. 1 hit. PF03404. Mo-co_dimer. 1 hit. PF00175. NAD_binding_1. 1 hit. PF00174. Oxidored_molyb. 1 hit. [Graphical view]
PIRSF	PIRSF000233. Nitr_rd_NADH. 1 hit.
PRINTS	PR00406. CYTB5RDTASE. PR00363. CYTOCHROMEB5. PR00407. EUMOPTERIN. PR00371. FPNCR.
ProDom	PD000612. Cyt_B5. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00191. CYTOCHROME_B5_1. 1 hit. PS50255. CYTOCHROME_B5_2. 1 hit. PS51384. FAD_FR. 1 hit. PS00559. MOLYBDOPTERIN_EUK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NIA7_HORVU

Accession

Primary (citable) accession number: P27968

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	June 16, 2009
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)