Reviewed, UniProtKB/Swiss-Prot P27967 (NIA1_HORVU)

Names and origin

Protein names	Recommended name:     Nitrate reductase [NADH]       Short name=NR     EC=1.7.1.1
Organism	Hordeum vulgare (Barley)
Taxonomic identifier	4513 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Hordeum

Protein attributes

Sequence length	915 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.
Catalytic activity	Nitrite + NAD+ + H2O = nitrate + NADH.
Cofactor	Binds 1 FAD per subunit. Binds 1 heme group per subunit. Binds 1 molybdenum-pterin group per subunit.
Subunit structure	Homodimer.
Sequence similarities	Belongs to the nitrate reductase family. Contains 1 cytochrome b5 heme-binding domain. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Biological process	Nitrate assimilation
Ligand	FAD Flavoprotein Heme Iron Metal-binding Molybdenum NAD
Molecular function	Oxidoreductase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	nitrate assimilation Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro molybdenum ion binding Inferred from electronic annotation. Source: UniProtKB-KW nitrate reductase (NADH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 915	915	Nitrate reductase [NADH]		PRO_0000166056
Regions
	Domain	538 – 613	76	Cytochrome b5 heme-binding
	Domain	654 – 767	114	FAD-binding FR-type
Sites
	Metal binding	189	1	Molybdenum-pterin Potential
	Metal binding	243	1	Molybdenum-pterin Potential
	Metal binding	573	1	Iron (heme axial ligand) By similarity
	Metal binding	596	1	Iron (heme axial ligand) By similarity
Amino acid modifications
	Disulfide bond	428		Interchain Potential

Sequences

	Sequence		Length	Mass (Da)
	P27967-1 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 937FFA96B6FDAA56	FASTA	915	101,770
	10 20 30 40 50 60 MAASVEPRQP FGRLDAPATA PTARAPGSNG IRRRADSPVR GCGFPSLISP PRKGPVAEEE 70 80 90 100 110 120 EDDDDEDDEG HEDWREAYGS HLQLEVEPST RDPRDEGTAD AWIERNPSLI RLTGKHPLNC 130 140 150 160 170 180 EPPLARLMHH GFITPAPLHY VRNHGAVPRG DWATWTVEVT GLVRRPARLT MDELANGFPA 190 200 210 220 230 240 AEVPATLVCA GNRRKEQNMV QQTVGFNWGA AGVSTSVWRG ARLRDVLLRC GVMSKKGQAL 250 260 270 280 290 300 NVCFEGAEDL PGGGGSKYGT SVSREWAMDP SRDIILPYAQ NGEPLLPDHG YPVRVLIPGC 310 320 330 340 350 360 IGGRMVKWVR RIVVTTAESD NYYHFKDNRV LPSHVDAELA NAEAWWYRPE YIINELNTNC 370 380 390 400 410 420 VITTPGHDEI LPINAFTTQR AYTIKGYAYS GGGKKITRVE VTLDGGESWM LCTLDIPEKP 430 440 450 460 470 480 NKYGRYWCWC FWSVEIEVLD LLGAKEVAVR TWDQTHNTQP EKLIWNLMGM MNNCWFKIKV 490 500 510 520 530 540 NVCRPHKGEI GLVFEHPTQP GNQTGGWMAR QKHLETAEAA APGLKRSTST PFMNTAGDKQ 550 560 570 580 590 600 FTMSEVRKHG SKESAWIVVH GHVYDCTAFL KDHPGGADSI LINAGSDCTE EFDAIHSDKA 610 620 630 640 650 660 KALLDTYRIG ELITTGTGYN SDNSVHGGSS LSHLAPIREA TKVAGAPIAL SSPREKVPCR 670 680 690 700 710 720 LVDKKELSHD VRLFRFALPS SDQVLGLPVG KHIFVCATID GKLCMRAYTP TSMVDEIGQF 730 740 750 760 770 780 ELLVKVYFRD EHPKFPNGGL MTQYLESLQV GSSYIDVKGP LGHVEYTGRG NFVINGKQRR 790 800 810 820 830 840 ARRLAMICGG SGITPMYQVI QAVLRDQPED ETEMHLVYAN RSEDDILLRD ELDRWAAEYP 850 860 870 880 890 900 DRLKVWYVID QVKRPEDGWK FSVGFVTEDI LRAHVPEGGD DTLALACGPP PMIKFAISPN 910 LEKMKYDMAN SFISF

References

[1]	"Analysis of barley nitrate reductase cDNA and genomic clones." Schnorr K.M., Juricek M., Huang C., Culley D., Kleinhofs A. Mol. Gen. Genet. 227:411-416(1991) [PubMed: 1865878] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Himalaya. Tissue: Leaf.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X57845 Genomic DNA. Translation: CAA40976.1.
PIR	RDBHNH. S17453.
3D structure databases
HSSP	HSSP built from PDB template 2CND based on UniProtKB P17571.
SMR	P27967. Positions 656-915.
ModBase	Search...
Organism-specific databases
Gramene	P27967.
Enzyme and pathway databases
BRENDA	1.7.1.1. 283.
Family and domain databases
InterPro	IPR001199. Cyt_B5. IPR018506. Cyt_B5_heme-BS. IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR005066. MoCF_OxRdtse_dimer. IPR008335. Mopterin_OxRdtase_euk. IPR001834. NADH-Cyt_B5_reductase. IPR012137. Nitr_rd_NADH. IPR008333. OxRdtase_FAD-bd. IPR001433. OxRdtase_FAD/NAD_bd. IPR000572. OxRdtase_Mopterin-bd. [Graphical view]
Gene3D	G3DSA:3.10.120.10. Cyt_B5. 1 hit. G3DSA:2.60.40.650. MoCF_oxrdtse_dimer. 1 hit. G3DSA:3.90.420.10. Oxred_molyb_bd. 1 hit.
Pfam	PF00173. Cyt-b5. 1 hit. PF00970. FAD_binding_6. 1 hit. PF03404. Mo-co_dimer. 1 hit. PF00175. NAD_binding_1. 1 hit. PF00174. Oxidored_molyb. 1 hit. [Graphical view]
PIRSF	PIRSF000233. Nitr_rd_NADH. 1 hit.
PRINTS	PR00406. CYTB5RDTASE. PR00363. CYTOCHROMEB5. PR00407. EUMOPTERIN. PR00371. FPNCR.
ProDom	PD000612. Cyt_B5. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00191. CYTOCHROME_B5_1. 1 hit. PS50255. CYTOCHROME_B5_2. 1 hit. PS51384. FAD_FR. 1 hit. PS00559. MOLYBDOPTERIN_EUK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NIA1_HORVU

Accession

Primary (citable) accession number: P27967

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	June 16, 2009
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)