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Protein

Nitrate reductase [NADH]

Gene
N/A
Organism
Hordeum vulgare (Barley)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.

Catalytic activityi

Nitrite + NAD+ + H2O = nitrate + NADH.

Cofactori

Protein has several cofactor binding sites:
  • FADBy similarityNote: Binds 1 FAD per subunit.By similarity
  • hemeBy similarityNote: Binds 1 heme group per subunit.By similarity
  • Mo-molybdopterinBy similarityNote: Binds 1 Mo-molybdopterin (Mo-MPT) cofactor per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi189MolybdenumBy similarity1
Metal bindingi573Iron (heme axial ligand)PROSITE-ProRule annotation1
Metal bindingi596Iron (heme axial ligand)PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processNitrate assimilation
LigandFAD, Flavoprotein, Heme, Iron, Metal-binding, Molybdenum, NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrate reductase [NADH] (EC:1.7.1.1)
Short name:
NR
OrganismiHordeum vulgare (Barley)
Taxonomic identifieri4513 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladePooideaeTriticodaeTriticeaeHordeinaeHordeum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001660561 – 915Nitrate reductase [NADH]Add BLAST915

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi428InterchainSequence analysis

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP27967

Expressioni

Gene expression databases

ExpressionAtlasiP27967 baseline and differential

Interactioni

Subunit structurei

Homodimer.

Structurei

3D structure databases

ProteinModelPortaliP27967
SMRiP27967
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini538 – 613Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd BLAST76
Domaini654 – 767FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST114

Sequence similaritiesi

Belongs to the nitrate reductase family.Curated

Phylogenomic databases

eggNOGiKOG0534 Eukaryota
KOG0535 Eukaryota
KOG0537 Eukaryota
COG0543 LUCA
COG2041 LUCA

Family and domain databases

Gene3Di3.10.120.10, 1 hit
3.90.420.10, 1 hit
InterProiView protein in InterPro
IPR001199 Cyt_B5-like_heme/steroid-bd
IPR036400 Cyt_B5-like_heme/steroid_sf
IPR018506 Cyt_B5_heme-BS
IPR017927 Fd_Rdtase_FAD-bd
IPR001709 Flavoprot_Pyr_Nucl_cyt_Rdtase
IPR014756 Ig_E-set
IPR005066 MoCF_OxRdtse_dimer
IPR008335 Mopterin_OxRdtase_euk
IPR001834 NADH-Cyt_B5_reductase
IPR012137 Nitr_rd_NADH
IPR008333 OxRdtase_FAD-bd_dom
IPR001433 OxRdtase_FAD/NAD-bd
IPR000572 OxRdtase_Mopterin-bd_dom
IPR036374 OxRdtase_Mopterin-bd_sf
IPR022407 OxRdtase_Mopterin_BS
IPR017938 Riboflavin_synthase-like_b-brl
PfamiView protein in Pfam
PF00173 Cyt-b5, 1 hit
PF00970 FAD_binding_6, 1 hit
PF03404 Mo-co_dimer, 1 hit
PF00175 NAD_binding_1, 1 hit
PF00174 Oxidored_molyb, 1 hit
PIRSFiPIRSF000233 Nitr_rd_NADH, 1 hit
PRINTSiPR00406 CYTB5RDTASE
PR00363 CYTOCHROMEB5
PR00407 EUMOPTERIN
PR00371 FPNCR
SMARTiView protein in SMART
SM01117 Cyt-b5, 1 hit
SUPFAMiSSF55856 SSF55856, 1 hit
SSF56524 SSF56524, 1 hit
SSF63380 SSF63380, 1 hit
SSF81296 SSF81296, 1 hit
PROSITEiView protein in PROSITE
PS00191 CYTOCHROME_B5_1, 1 hit
PS50255 CYTOCHROME_B5_2, 1 hit
PS51384 FAD_FR, 1 hit
PS00559 MOLYBDOPTERIN_EUK, 1 hit

Sequencei

Sequence statusi: Complete.

P27967-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAASVEPRQP FGRLDAPATA PTARAPGSNG IRRRADSPVR GCGFPSLISP
60 70 80 90 100
PRKGPVAEEE EDDDDEDDEG HEDWREAYGS HLQLEVEPST RDPRDEGTAD
110 120 130 140 150
AWIERNPSLI RLTGKHPLNC EPPLARLMHH GFITPAPLHY VRNHGAVPRG
160 170 180 190 200
DWATWTVEVT GLVRRPARLT MDELANGFPA AEVPATLVCA GNRRKEQNMV
210 220 230 240 250
QQTVGFNWGA AGVSTSVWRG ARLRDVLLRC GVMSKKGQAL NVCFEGAEDL
260 270 280 290 300
PGGGGSKYGT SVSREWAMDP SRDIILPYAQ NGEPLLPDHG YPVRVLIPGC
310 320 330 340 350
IGGRMVKWVR RIVVTTAESD NYYHFKDNRV LPSHVDAELA NAEAWWYRPE
360 370 380 390 400
YIINELNTNC VITTPGHDEI LPINAFTTQR AYTIKGYAYS GGGKKITRVE
410 420 430 440 450
VTLDGGESWM LCTLDIPEKP NKYGRYWCWC FWSVEIEVLD LLGAKEVAVR
460 470 480 490 500
TWDQTHNTQP EKLIWNLMGM MNNCWFKIKV NVCRPHKGEI GLVFEHPTQP
510 520 530 540 550
GNQTGGWMAR QKHLETAEAA APGLKRSTST PFMNTAGDKQ FTMSEVRKHG
560 570 580 590 600
SKESAWIVVH GHVYDCTAFL KDHPGGADSI LINAGSDCTE EFDAIHSDKA
610 620 630 640 650
KALLDTYRIG ELITTGTGYN SDNSVHGGSS LSHLAPIREA TKVAGAPIAL
660 670 680 690 700
SSPREKVPCR LVDKKELSHD VRLFRFALPS SDQVLGLPVG KHIFVCATID
710 720 730 740 750
GKLCMRAYTP TSMVDEIGQF ELLVKVYFRD EHPKFPNGGL MTQYLESLQV
760 770 780 790 800
GSSYIDVKGP LGHVEYTGRG NFVINGKQRR ARRLAMICGG SGITPMYQVI
810 820 830 840 850
QAVLRDQPED ETEMHLVYAN RSEDDILLRD ELDRWAAEYP DRLKVWYVID
860 870 880 890 900
QVKRPEDGWK FSVGFVTEDI LRAHVPEGGD DTLALACGPP PMIKFAISPN
910
LEKMKYDMAN SFISF
Length:915
Mass (Da):101,770
Last modified:August 1, 1992 - v1
Checksum:i937FFA96B6FDAA56
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57845 Genomic DNA Translation: CAA40976.1
PIRiS17453 RDBHNH

Similar proteinsi

Entry informationi

Entry nameiNIA1_HORVU
AccessioniPrimary (citable) accession number: P27967
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: May 23, 2018
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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