##gff-version 3
P27949	UniProtKB	Chain	1	215	.	.	.	ID=PRO_0000082591;Note=Ubiquitin-conjugating enzyme E2	
P27949	UniProtKB	Domain	1	160	.	.	.	Note=UBC core;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00388	
P27949	UniProtKB	Active site	85	85	.	.	.	Note=Glycyl thioester intermediate;Ontology_term=ECO:0000255,ECO:0000255,ECO:0000269;evidence=ECO:0000255|PROSITE-ProRule:PRU00388,ECO:0000255|PROSITE-ProRule:PRU10133,ECO:0000269|PubMed:29472632;Dbxref=PMID:29472632	
P27949	UniProtKB	Mutagenesis	85	85	.	.	.	Note=Complete loss of thioester bond with ubiquitin. No effect on the NF-kappa-B and iNF inhibitory function. C->A;Ontology_term=ECO:0000269,ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:29472632,ECO:0000269|PubMed:34204411,ECO:0000269|PubMed:34759016;Dbxref=PMID:29472632,PMID:34204411,PMID:34759016	
P27949	UniProtKB	Mutagenesis	162	162	.	.	.	Note=No effet on the formation of the thioester bond with ubiquitin. C->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:29472632;Dbxref=PMID:29472632	
P27949	UniProtKB	Mutagenesis	189	189	.	.	.	Note=No effet on the formation of the thioester bond with ubiquitin. C->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:29472632;Dbxref=PMID:29472632