P27949 (UBC_ASFB7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin-conjugating enzyme E2
EC=6.3.2.19

Alternative name(s):
Ubiquitin carrier protein
Ubiquitin-protein ligase

Gene names

Name:	UBC
Ordered Locus Names:	BA71V-144
ORF Names:	I215L

Organism

African swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V) (ASFV) [Reference proteome]

Taxonomic identifier

10498 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Asfarviridae › Asfivirus › African swine fever virus

Virus host

Ornithodoros (relapsing fever ticks) [TaxID: 6937]
Sus scrofa (Pig) [TaxID: 9823]

Protein attributes

Sequence length	215 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Performs the second step in the ubiquitination reaction that targets specifically a protein for degradation via the proteasome By similarity.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Induction	Mainly late in infection.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Developmental stage	Late protein
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 215	215	Ubiquitin-conjugating enzyme E2		PRO_0000082591
Regions
	Compositional bias	183 – 215	33	Asp/Glu-rich (acidic)
Sites
	Active site	85	1	Glycyl thioester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P27949 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 3488BEC687E67727	FASTA	215	24,737
	10 20 30 40 50 60 MVSRFLIAEY RHLIENPSEN FKISVNENNI TEWDVILRGP PDTLYEGGLF KAKVAFPPEY 70 80 90 100 110 120 PYAPPKLTFT SEMWHPNIYP DGRLCISILH GDNAEEQGMT WSPAQKIDTI LLSVISLLNE 130 140 150 160 170 180 PNPDSPANVD AAKSYRKYVY KEDLESYPME VKKTVKKSLD ECSPEDIEYF KNAASNVPPI 190 200 210 PSDAYEDECE EMEDDTYILT YDDDEEEEDE EMDDE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genes homologous to ubiquitin-conjugating proteins and eukaryotic transcription factor SII in African swine fever virus." Rodriguez J.M., Salas M.L., Vinuela E. Virology 186:40-52(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Analysis of the complete nucleotide sequence of African swine fever virus." Yanez R.J., Rodriguez J.M., Nogal M.L., Yuste L., Enriquez C., Rodriguez J.F., Vinuela E. Virology 208:249-278(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M77121 Genomic DNA. Translation: AAA42704.1. U18466 Genomic DNA. Translation: AAA65370.1.
PIR	UQXFAS. F39448.
RefSeq	NP_042834.1. NC_001659.1.
3D structure databases
ProteinModelPortal	P27949.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1488905.
Phylogenomic databases
ProtClustDB	CLSP2510037.
Enzyme and pathway databases
UniPathway	UPA00143.
Family and domain databases
Gene3D	3.10.110.10. 1 hit.
InterPro	IPR000608. UBQ-conjugat_E2. IPR023313. UBQ-conjugating_AS. IPR016135. UBQ-conjugating_enzyme/RWD. [Graphical view]
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
SUPFAM	SSF54495. UBQ-conjugat/RWD-like. 1 hit.
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBC_ASFB7

Accession

Primary (citable) accession number: P27949

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	April 3, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program