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P27934 (AMY3E_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-amylase isozyme 3E
EC=3.2.1.1
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase
Gene names
Name:AMY1.4
Synonyms:AMY3E
Ordered Locus Names:Os08g0473600, LOC_Os08g36900
ORF Names:P0013B04.34-1, P0451G12.3-1
OrganismOryza sativa subsp. japonica (Rice)
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length437 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Important for breakdown of endosperm starch during germination.

Catalytic activity

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.

Cofactor

Binds 3 calcium ions per subunit By similarity.

Subunit structure

Monomer.

Tissue specificity

More abundant in germinating seeds than in young roots, young leaves and callus.

Developmental stage

Expressed at a high level during germination in the aleurones cells under the control of the plant hormone gibberellic acid and in the developing grains at a low level.

Miscellaneous

Binds starch not only at the active site, but also via accessory binding sites on the protein surface that are important for efficient binding to starch granules and thereby increase enzyme activity By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 13 family.

Sequence caution

The sequence BAD73794.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence BAD73796.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
   Coding sequence diversityAlternative splicing
   DomainSignal
   LigandCalcium
Metal-binding
   Molecular functionGlycosidase
Hydrolase
Gene Ontology (GO)
   Biological processcarbohydrate metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionalpha-amylase activity

Inferred from electronic annotation. Source: EC

calcium ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P27934-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P27934-2)

The sequence of this isoform differs from the canonical sequence as follows:
     224-233: TFVVGEIWSS → ASWGGCPIRP
     234-437: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Chain26 – 437412Alpha-amylase isozyme 3E
PRO_0000001416

Regions

Region76 – 772Substrate binding By similarity
Region202 – 2076Substrate binding By similarity
Region298 – 3003Substrate binding By similarity
Region401 – 4033Substrate binding By similarity

Sites

Active site2041Nucleophile By similarity
Active site2291Proton donor By similarity
Metal binding1161Calcium 1 By similarity
Metal binding1331Calcium 2 By similarity
Metal binding1361Calcium 2; via carbonyl oxygen By similarity
Metal binding1381Calcium 2; via carbonyl oxygen By similarity
Metal binding1421Calcium 2 By similarity
Metal binding1521Calcium 3 By similarity
Metal binding1631Calcium 1 By similarity
Metal binding1661Calcium 1; via carbonyl oxygen By similarity
Metal binding1681Calcium 3; via carbonyl oxygen By similarity
Metal binding1711Calcium 3; via carbonyl oxygen By similarity
Metal binding1731Calcium 1 By similarity
Metal binding1731Calcium 3 By similarity
Metal binding2081Calcium 1; via carbonyl oxygen By similarity
Binding site2311Substrate By similarity
Binding site2331Substrate By similarity
Binding site2511Substrate By similarity
Binding site2581Substrate By similarity
Binding site2921Substrate By similarity
Binding site3111Substrate By similarity
Binding site3171Substrate By similarity
Binding site3961Substrate By similarity
Binding site4231Substrate By similarity
Site3121Transition state stabilizer By similarity

Natural variations

Alternative sequence224 – 23310TFVVGEIWSS → ASWGGCPIRP in isoform 2.
VSP_016022
Alternative sequence234 – 437204Missing in isoform 2.
VSP_016023

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified August 1, 1992. Version 1.
Checksum: C0E8276CCEA16602

FASTA43748,708
        10         20         30         40         50         60 
MGKHHVTLCC VVFAVLCLAS SLAQAQVLFQ GFNWESWRKQ GGWYNFLHEK VEEIASTGAT 

        70         80         90        100        110        120 
HVWLPPPSHS VSPQGYMPGR LYDLDASKYG TEAELKSLIE AFHDKNVECL ADIVINHRCA 

       130        140        150        160        170        180 
DYKDSRGVYC VFEGGTPDGR LDWGPDMICS DDTQYSNGRG HRDTGAGFGA APDIDHLNPR 

       190        200        210        220        230        240 
VQRELTDWLN WLRTDLGFDG WRLDFAKGYS APLARIYVDN TNPTFVVGEI WSSLIYNGDG 

       250        260        270        280        290        300 
KPSTNQDADR QELVNWVEGV GKPATAFDFT TKGILQAAVQ GELWRLHDGN GKAPGLMGWM 

       310        320        330        340        350        360 
PDQAVTFVDN HDTGSTQSLW PFPSDKVMQG YAYILTHPGI PCIFYDHVFD WNLQHEIATL 

       370        380        390        400        410        420 
AEIRSRNGIH AESTLDILKA EGDIYVAMID GKVITKLGPR YDAGGIIPSD FHVVAHGNDY 

       430 
CVWEKEGLRV PAGRKHY

« Hide

Isoform 2 [UniParc].

Checksum: 4EFAC00EB1B5078A
Show »

FASTA23326,038

References

« Hide 'large scale' references
[1]"Structural organization and differential expression of rice alpha-amylase genes."
Huang N., Koizumi N., Reinl S.J., Rodriguez R.L.
Nucleic Acids Res. 18:7007-7014(1990) [PubMed: 2263460] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. M202.
Tissue: Etiolated leaf.
[2]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed: 16100779] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[3]"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
The rice full-length cDNA consortium
Science 301:376-379(2003) [PubMed: 12869764] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: cv. Nipponbare.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M59352 Genomic DNA. Translation: AAA33896.1.
AP004399 Genomic DNA. Translation: BAD09334.1.
AP004399 Genomic DNA. Translation: BAD73794.1. Sequence problems.
AP004457 Genomic DNA. Translation: BAD09374.1.
AP004457 Genomic DNA. Translation: BAD73796.1. Sequence problems.
AK064071 mRNA. No translation available.
AK103413 mRNA. No translation available.
PIRJT0946.
RefSeqNP_001062023.1. NM_001068558.1.
UniGeneOs.12593.

3D structure databases

ProteinModelPortalP27934.
SMRP27934. Positions 26-425.
ModBaseSearch...

Protein-protein interaction databases

STRINGP27934.

Protein family/group databases

CAZyGH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsLOC_Os08g36900.1; LOC_Os08g36900.1; LOC_Os08g36900.
GeneID4345812.
KEGGosa:4345812.

Organism-specific databases

GrameneP27934.

Phylogenomic databases

OMAYLETETY.
PhylomeDBP27934.
ProtClustDBCLSN2697397.

Family and domain databases

InterProIPR012850. A-amylase_bs_C.
IPR013775. A-amylase_pln.
IPR015902. Alpha_amylase.
IPR006046. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_subgr_catalytic.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
Gene3DG3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit.
G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
KOK01176.
PANTHERPTHR10357. Alpha_amylase. 1 hit.
PfamPF07821. Alpha-amyl_C2. 1 hit.
PF00128. Alpha-amylase. 1 hit.
[Graphical view]
PIRSFPIRSF001028. Alph-amls_plant. 1 hit.
PRINTSPR00110. ALPHAAMYLASE.
SMARTSM00642. Aamy. 1 hit.
SM00810. Alpha-amyl_C2. 1 hit.
[Graphical view]
SUPFAMSSF51445. Glyco_hydro_cat. 1 hit.
ProtoNetSearch...

Entry information

Entry nameAMY3E_ORYSJ
AccessionPrimary (citable) accession number: P27934
Secondary accession number(s): Q5QLB2, Q6ZDD6
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: December 14, 2011
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents