Reviewed, UniProtKB/Swiss-Prot P27932 (AMY3A_ORYSJ)

Names and origin

Protein names

Recommended name:
    Alpha-amylase isozyme 3A
    EC=3.2.1.1
Alternative name(s):
    1,4-alpha-D-glucan glucanohydrolase

Gene names

Name:	AMY1.2
Synonyms:	AMY3A
Ordered Locus Names:	Os09g0457400, LOC_Os09g28400
ORF Names:	B1045B05.8

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	440 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Important for breakdown of endosperm starch during germination.
Catalytic activity	Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in oligosaccharides and polysaccharides.
Cofactor	Binds 3 calcium ions per subunit By similarity.
Subunit structure	Monomer.
Tissue specificity	Most abundant in embryo-derived callus tissue.
Developmental stage	Expressed at a high level during germination in the aleurones cells under the control of the plant hormone gibberellic acid and in the developing grains at a low level.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alpha-amylase activity Ref.1 Inferred from expression pattern. Source: Gramene calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 440	414	Alpha-amylase isozyme 3A		PRO_0000001412
Sites
	Active site	207	1	Nucleophile By similarity
	Active site	232	1	Proton donor By similarity
	Active site	315	1	By similarity
	Metal binding	119	1	Calcium 1 By similarity
	Metal binding	145	1	Calcium 2 By similarity
	Metal binding	155	1	Calcium 3 By similarity
	Metal binding	166	1	Calcium 3 By similarity
	Metal binding	169	1	Calcium 3; via carbonyl oxygen By similarity
	Metal binding	170	1	Calcium 1 By similarity
	Metal binding	171	1	Calcium 1; via carbonyl oxygen By similarity
	Metal binding	174	1	Calcium 3; via carbonyl oxygen By similarity
	Metal binding	176	1	Calcium 1 By similarity
	Metal binding	176	1	Calcium 3 By similarity
Experimental info
	Sequence conflict	350	1	H → Q in CAA39776. Ref.1
	Sequence conflict	405	1	D → E in CAA39776. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P27932-1 [UniParc]. Last modified October 25, 2005. Version 2. Checksum: 8AAB604A29222533	FASTA	440	48,868
	10 20 30 40 50 60 MGKQMAALCG FLLVALLWLT PDVAHAQTQI LFQGFNWDSW KKQGGWYNML KDQVGDIASA 70 80 90 100 110 120 GVTHVWLPPP THSVSPQGYM PGRLYDLNAS KYGTKAELKS LIAAFHAKGI KCVADIVVNH 130 140 150 160 170 180 RCADDKDGRG VYCIFKGGGP RGCLDWGPSM ICCDDTQYSD GTGHRDTGAD FAAAPDIDHL 190 200 210 220 230 240 NPLVQRELSD WLRWLRRDVG FDGWRLDFAK GYSAAVARTY VQNARPSFVV AEIWNSLSYD 250 260 270 280 290 300 GDGKPAANQD GQRQELVNWV KQVGGPATAF DFTTKGILQS AVQGELWRMR DKDGKAPGMI 310 320 330 340 350 360 GWYPEKAVTF VDNHDTGSTQ RMWPFPSDKV ILGYAYILTH PGVPCIFYDH VFDWNLKQEI 370 380 390 400 410 420 NALAATRKRN GINAGSKLRV LAAESDMYVA MVDERVITKI GPRIDVGNII PSDFHIVAHG 430 440 NDYCVWEKSG LRVPEPEGRR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of an alpha-amylase multigene cluster in rice." Sutliff T.D., Huang N., Litts J.C., Rodriguez R.L. Plant Mol. Biol. 16:579-591(1991) [PubMed: 1714318] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. M202. Tissue: Etiolated leaf.
[2]	"Oryza sativa nipponbare chromosome 9 genomic DNA sequence." The international rice genome sequencing project (IRGSP) consortium Submitted (NOV-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X56336 Genomic DNA. Translation: CAA39776.1. AP005891 Genomic DNA. Translation: BAD38366.1. AK099330 mRNA. No translation available.
PIR	S14958.
RefSeq	NP_001063367.1.
UniGene	Os.51838
3D structure databases
HSSP	HSSP built from PDB template 1AVA based on UniProtKB P04063.
ModBase	Search...
Protein family/group databases
CAZy	GH13. Glycoside Hydrolase Family 13.
Genome annotation databases
GeneID	4347262.
KEGG	osa:4347262.
Organism-specific databases
Gramene	P27932.
Family and domain databases
InterPro	IPR012850. A-amylase_bs_C. IPR013775. A-amylase_pln. IPR006046. Glyco_hydro_13. IPR006047. Glyco_hydro_13_cat. IPR006589. Glyco_hydro_13_sub_cat. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF07821. Alpha-amyl_C2. 1 hit. PF00128. Alpha-amylase. 1 hit. [Graphical view]
PIRSF	PIRSF001028. Alph-amls_plant. 1 hit.
PRINTS	PR00110. ALPHAAMYLASE.
SMART	SM00642. Aamy. 1 hit. SM00810. Alpha-amyl_C2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMY3A_ORYSJ

Accession

Primary (citable) accession number: P27932
Secondary accession number(s): Q67U05

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	October 25, 2005
Last modified:	June 16, 2009
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)