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P27929

- NAM9_YEAST

UniProt

P27929 - NAM9_YEAST

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Protein

37S ribosomal protein NAM9, mitochondrial

Gene

NAM9

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  1. rRNA binding Source: UniProtKB-KW
  2. structural constituent of ribosome Source: SGD

GO - Biological processi

  1. mitochondrial translation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Keywords - Ligandi

RNA-binding, rRNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33156-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
37S ribosomal protein NAM9, mitochondrial
Alternative name(s):
Nuclear accommodation of mitochondria protein 9
Gene namesi
Name:NAM9
Synonyms:MNA6
Ordered Locus Names:YNL137C
ORF Names:N1211, N1840
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNL137c.
SGDiS000005081. NAM9.

Subcellular locationi

Mitochondrion 3 Publications

GO - Cellular componenti

  1. mitochondrial small ribosomal subunit Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi82 – 821S → L in NAM9-1; suppressor for ocher mutations in mitochondrial DNA, possibly through decreasing the fidelity of translation. 1 Publication
Mutagenesisi109 – 1091L → F in MNA6-3; causes temperature-dependent loss of the 15S rRNA. 1 Publication
Mutagenesisi111 – 1111R → K in MNA6-1; causes temperature-dependent loss of the 15S rRNA. 1 Publication
Mutagenesisi424 – 4241P → L in MNA6-4; causes temperature-dependent loss of the 15S rRNA. 1 Publication
Mutagenesisi438 – 4381P → L in MNA6-2; causes temperature-dependent loss of the 15S rRNA. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3434MitochondrionSequence AnalysisAdd
BLAST
Chaini35 – 48645237S ribosomal protein NAM9, mitochondrialPRO_0000030639Add
BLAST

Proteomic databases

MaxQBiP27929.
PaxDbiP27929.
PeptideAtlasiP27929.

Expressioni

Gene expression databases

GenevestigatoriP27929.

Interactioni

Subunit structurei

Component of the mitochondrial small ribosomal subunit. Mature mitochondrial ribosomes consist of a small (37S) and a large (54S) subunit. The 37S subunit contains at least 33 different proteins and 1 molecule of RNA (15S). The 54S subunit contains at least 45 different proteins and 1 molecule of RNA (21S).3 Publications

Protein-protein interaction databases

BioGridi35689. 44 interactions.
DIPiDIP-6702N.
IntActiP27929. 32 interactions.
MINTiMINT-652493.
STRINGi4932.YNL137C.

Structurei

3D structure databases

ProteinModelPortaliP27929.
SMRiP27929. Positions 42-153.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini103 – 17270S4 RNA-bindingPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ribosomal protein S4P family.Curated
Contains 1 S4 RNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0522.
HOGENOMiHOG000209594.
InParanoidiP27929.
OMAiFRAMFAS.
OrthoDBiEOG7HB5K7.

Family and domain databases

Gene3Di3.10.290.10. 1 hit.
InterProiIPR022801. Ribosomal_S4/S9.
IPR018079. Ribosomal_S4_CS.
IPR002942. S4_RNA-bd.
[Graphical view]
PANTHERiPTHR11831. PTHR11831. 1 hit.
PfamiPF01479. S4. 1 hit.
[Graphical view]
SMARTiSM00363. S4. 1 hit.
[Graphical view]
PROSITEiPS00632. RIBOSOMAL_S4. 1 hit.
PS50889. S4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27929-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPRKANLLKS LARGRVRTSF NKYNLFNLYK KGGVDLKSKS LYQQKWTAKQ
60 70 80 90 100
ETRAYHGEHL TEKRWQTVFK PKLDSVAQLD ASLRGGEIKE TPFLLQTFAV
110 120 130 140 150
LEKRLDFALF RAMFASSVRQ ARQFILHGNV RVNGVKIKHP SYTLKPGDMF
160 170 180 190 200
SVKPDKVLEA LGAKKPSFQE ALKIDKTQIV LWNKYVKEAK TEPKEVWEKK
210 220 230 240 250
LENFEKMSDS NPKKLQFQEF LRQYNKNLES QQYNALKGCT QEGILRKLLN
260 270 280 290 300
VEKEIGKSNN EPLSIDELKQ GLPEIQDSQL LESLNNAYQE FFKSGEIRRE
310 320 330 340 350
IISKCQPDEL ISLATEMMNP NETTKKELSD GAKSALRSGK RIIAESVKLW
360 370 380 390 400
TKNITDHFKT RMSDISDGSL TFDPKWAKNL KYHDPIKLSE LEGDEPKARK
410 420 430 440 450
LINLPWQKNY VYGRQDPKKP FFTPWKPRPF LSPFAILPHH LEISFKTCHA
460 470 480
VYLRDPVARP GQSEVISPFD VPVHERAYMY YLRNGK
Length:486
Mass (Da):56,356
Last modified:February 1, 1996 - v2
Checksum:i4A5CB85B354955D1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M60730 Unassigned DNA. Translation: AAA19439.1.
Z46843 Genomic DNA. Translation: CAA86888.1.
Z71413 Genomic DNA. Translation: CAA96019.1.
BK006947 Genomic DNA. Translation: DAA10411.1.
PIRiS55146.
RefSeqiNP_014262.3. NM_001182975.3.

Genome annotation databases

EnsemblFungiiYNL137C; YNL137C; YNL137C.
GeneIDi855585.
KEGGisce:YNL137C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M60730 Unassigned DNA. Translation: AAA19439.1 .
Z46843 Genomic DNA. Translation: CAA86888.1 .
Z71413 Genomic DNA. Translation: CAA96019.1 .
BK006947 Genomic DNA. Translation: DAA10411.1 .
PIRi S55146.
RefSeqi NP_014262.3. NM_001182975.3.

3D structure databases

ProteinModelPortali P27929.
SMRi P27929. Positions 42-153.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35689. 44 interactions.
DIPi DIP-6702N.
IntActi P27929. 32 interactions.
MINTi MINT-652493.
STRINGi 4932.YNL137C.

Proteomic databases

MaxQBi P27929.
PaxDbi P27929.
PeptideAtlasi P27929.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNL137C ; YNL137C ; YNL137C .
GeneIDi 855585.
KEGGi sce:YNL137C.

Organism-specific databases

CYGDi YNL137c.
SGDi S000005081. NAM9.

Phylogenomic databases

eggNOGi COG0522.
HOGENOMi HOG000209594.
InParanoidi P27929.
OMAi FRAMFAS.
OrthoDBi EOG7HB5K7.

Enzyme and pathway databases

BioCyci YEAST:G3O-33156-MONOMER.

Miscellaneous databases

NextBioi 979718.

Gene expression databases

Genevestigatori P27929.

Family and domain databases

Gene3Di 3.10.290.10. 1 hit.
InterProi IPR022801. Ribosomal_S4/S9.
IPR018079. Ribosomal_S4_CS.
IPR002942. S4_RNA-bd.
[Graphical view ]
PANTHERi PTHR11831. PTHR11831. 1 hit.
Pfami PF01479. S4. 1 hit.
[Graphical view ]
SMARTi SM00363. S4. 1 hit.
[Graphical view ]
PROSITEi PS00632. RIBOSOMAL_S4. 1 hit.
PS50889. S4. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "NAM9 nuclear suppressor of mitochondrial ochre mutations in Saccharomyces cerevisiae codes for a protein homologous to S4 ribosomal proteins from chloroplasts, bacteria, and eucaryotes."
    Boguta M., Dmochowska A., Borsuk P., Wrobel K., Gargouri A., Lazowska J., Slonimski P.P., Szczesniak B., Kruszewska A.
    Mol. Cell. Biol. 12:402-412(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  2. Boguta M.
    Submitted (JUN-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  3. "A 43.5 kb segment of yeast chromosome XIV, which contains MFA2, MEP2, CAP/SRV2, NAM9, FKB1/FPR1/RBP1, MOM22 and CPT1, predicts an adenosine deaminase gene and 14 new open reading frames."
    Mallet L., Bussereau F., Jacquet M.
    Yeast 11:1195-1209(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. "The NAM9-1 suppressor mutation in a nuclear gene encoding ribosomal mitochondrial protein of Saccharomyces cerevisiae."
    Dmochowska A., Konopinska A., Krzymowska M., Szczesniak B., Boguta M.
    Gene 162:81-85(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF SER-82.
  7. "The single amino acid changes in the yeast mitochondrial S4 ribosomal protein cause temperature-sensitive defect in the accumulation of mitochondrial 15S rRNA."
    Biswas T.K., Getz G.S.
    Biochemistry 38:13042-13054(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, SUBCELLULAR LOCATION, MUTAGENESIS OF LEU-109; ARG-111; PRO-424 AND PRO-438.
  8. "Identification of 12 new yeast mitochondrial ribosomal proteins including 6 that have no prokaryotic homologues."
    Saveanu C., Fromont-Racine M., Harington A., Ricard F., Namane A., Jacquier A.
    J. Biol. Chem. 276:15861-15867(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE MITOCHONDRIAL RIBOSOMAL SMALL COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  9. "Tag-mediated isolation of yeast mitochondrial ribosome and mass spectrometric identification of its new components."
    Gan X., Kitakawa M., Yoshino K., Oshiro N., Yonezawa K., Isono K.
    Eur. J. Biochem. 269:5203-5214(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE MITOCHONDRIAL RIBOSOMAL SMALL COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  10. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  11. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  12. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: ATCC 76625 / YPH499.

Entry informationi

Entry nameiNAM9_YEAST
AccessioniPrimary (citable) accession number: P27929
Secondary accession number(s): D6W145
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: February 1, 1996
Last modified: October 29, 2014
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3270 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Ribosomal proteins
    Ribosomal proteins families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3