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P27929

- NAM9_YEAST

UniProt

P27929 - NAM9_YEAST

Protein

37S ribosomal protein NAM9, mitochondrial

Gene

NAM9

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 2 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    GO - Molecular functioni

    1. rRNA binding Source: UniProtKB-KW
    2. structural constituent of ribosome Source: SGD

    GO - Biological processi

    1. mitochondrial translation Source: SGD

    Keywords - Molecular functioni

    Ribonucleoprotein, Ribosomal protein

    Keywords - Ligandi

    RNA-binding, rRNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33156-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    37S ribosomal protein NAM9, mitochondrial
    Alternative name(s):
    Nuclear accommodation of mitochondria protein 9
    Gene namesi
    Name:NAM9
    Synonyms:MNA6
    Ordered Locus Names:YNL137C
    ORF Names:N1211, N1840
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNL137c.
    SGDiS000005081. NAM9.

    Subcellular locationi

    Mitochondrion 3 Publications

    GO - Cellular componenti

    1. mitochondrial small ribosomal subunit Source: SGD

    Keywords - Cellular componenti

    Mitochondrion

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi82 – 821S → L in NAM9-1; suppressor for ocher mutations in mitochondrial DNA, possibly through decreasing the fidelity of translation. 1 Publication
    Mutagenesisi109 – 1091L → F in MNA6-3; causes temperature-dependent loss of the 15S rRNA. 1 Publication
    Mutagenesisi111 – 1111R → K in MNA6-1; causes temperature-dependent loss of the 15S rRNA. 1 Publication
    Mutagenesisi424 – 4241P → L in MNA6-4; causes temperature-dependent loss of the 15S rRNA. 1 Publication
    Mutagenesisi438 – 4381P → L in MNA6-2; causes temperature-dependent loss of the 15S rRNA. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3434MitochondrionSequence AnalysisAdd
    BLAST
    Chaini35 – 48645237S ribosomal protein NAM9, mitochondrialPRO_0000030639Add
    BLAST

    Proteomic databases

    MaxQBiP27929.
    PaxDbiP27929.
    PeptideAtlasiP27929.

    Expressioni

    Gene expression databases

    GenevestigatoriP27929.

    Interactioni

    Subunit structurei

    Component of the mitochondrial small ribosomal subunit. Mature mitochondrial ribosomes consist of a small (37S) and a large (54S) subunit. The 37S subunit contains at least 33 different proteins and 1 molecule of RNA (15S). The 54S subunit contains at least 45 different proteins and 1 molecule of RNA (21S).3 Publications

    Protein-protein interaction databases

    BioGridi35689. 44 interactions.
    DIPiDIP-6702N.
    IntActiP27929. 32 interactions.
    MINTiMINT-652493.
    STRINGi4932.YNL137C.

    Structurei

    3D structure databases

    ProteinModelPortaliP27929.
    SMRiP27929. Positions 42-153.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini103 – 17270S4 RNA-bindingPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ribosomal protein S4P family.Curated
    Contains 1 S4 RNA-binding domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0522.
    HOGENOMiHOG000209594.
    OMAiFRAMFAS.
    OrthoDBiEOG7HB5K7.

    Family and domain databases

    Gene3Di3.10.290.10. 1 hit.
    InterProiIPR022801. Ribosomal_S4/S9.
    IPR018079. Ribosomal_S4_CS.
    IPR002942. S4_RNA-bd.
    [Graphical view]
    PANTHERiPTHR11831. PTHR11831. 1 hit.
    PfamiPF01479. S4. 1 hit.
    [Graphical view]
    SMARTiSM00363. S4. 1 hit.
    [Graphical view]
    PROSITEiPS00632. RIBOSOMAL_S4. 1 hit.
    PS50889. S4. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P27929-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPRKANLLKS LARGRVRTSF NKYNLFNLYK KGGVDLKSKS LYQQKWTAKQ    50
    ETRAYHGEHL TEKRWQTVFK PKLDSVAQLD ASLRGGEIKE TPFLLQTFAV 100
    LEKRLDFALF RAMFASSVRQ ARQFILHGNV RVNGVKIKHP SYTLKPGDMF 150
    SVKPDKVLEA LGAKKPSFQE ALKIDKTQIV LWNKYVKEAK TEPKEVWEKK 200
    LENFEKMSDS NPKKLQFQEF LRQYNKNLES QQYNALKGCT QEGILRKLLN 250
    VEKEIGKSNN EPLSIDELKQ GLPEIQDSQL LESLNNAYQE FFKSGEIRRE 300
    IISKCQPDEL ISLATEMMNP NETTKKELSD GAKSALRSGK RIIAESVKLW 350
    TKNITDHFKT RMSDISDGSL TFDPKWAKNL KYHDPIKLSE LEGDEPKARK 400
    LINLPWQKNY VYGRQDPKKP FFTPWKPRPF LSPFAILPHH LEISFKTCHA 450
    VYLRDPVARP GQSEVISPFD VPVHERAYMY YLRNGK 486
    Length:486
    Mass (Da):56,356
    Last modified:February 1, 1996 - v2
    Checksum:i4A5CB85B354955D1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M60730 Unassigned DNA. Translation: AAA19439.1.
    Z46843 Genomic DNA. Translation: CAA86888.1.
    Z71413 Genomic DNA. Translation: CAA96019.1.
    BK006947 Genomic DNA. Translation: DAA10411.1.
    PIRiS55146.
    RefSeqiNP_014262.3. NM_001182975.3.

    Genome annotation databases

    EnsemblFungiiYNL137C; YNL137C; YNL137C.
    GeneIDi855585.
    KEGGisce:YNL137C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M60730 Unassigned DNA. Translation: AAA19439.1 .
    Z46843 Genomic DNA. Translation: CAA86888.1 .
    Z71413 Genomic DNA. Translation: CAA96019.1 .
    BK006947 Genomic DNA. Translation: DAA10411.1 .
    PIRi S55146.
    RefSeqi NP_014262.3. NM_001182975.3.

    3D structure databases

    ProteinModelPortali P27929.
    SMRi P27929. Positions 42-153.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35689. 44 interactions.
    DIPi DIP-6702N.
    IntActi P27929. 32 interactions.
    MINTi MINT-652493.
    STRINGi 4932.YNL137C.

    Proteomic databases

    MaxQBi P27929.
    PaxDbi P27929.
    PeptideAtlasi P27929.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL137C ; YNL137C ; YNL137C .
    GeneIDi 855585.
    KEGGi sce:YNL137C.

    Organism-specific databases

    CYGDi YNL137c.
    SGDi S000005081. NAM9.

    Phylogenomic databases

    eggNOGi COG0522.
    HOGENOMi HOG000209594.
    OMAi FRAMFAS.
    OrthoDBi EOG7HB5K7.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33156-MONOMER.

    Miscellaneous databases

    NextBioi 979718.

    Gene expression databases

    Genevestigatori P27929.

    Family and domain databases

    Gene3Di 3.10.290.10. 1 hit.
    InterProi IPR022801. Ribosomal_S4/S9.
    IPR018079. Ribosomal_S4_CS.
    IPR002942. S4_RNA-bd.
    [Graphical view ]
    PANTHERi PTHR11831. PTHR11831. 1 hit.
    Pfami PF01479. S4. 1 hit.
    [Graphical view ]
    SMARTi SM00363. S4. 1 hit.
    [Graphical view ]
    PROSITEi PS00632. RIBOSOMAL_S4. 1 hit.
    PS50889. S4. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "NAM9 nuclear suppressor of mitochondrial ochre mutations in Saccharomyces cerevisiae codes for a protein homologous to S4 ribosomal proteins from chloroplasts, bacteria, and eucaryotes."
      Boguta M., Dmochowska A., Borsuk P., Wrobel K., Gargouri A., Lazowska J., Slonimski P.P., Szczesniak B., Kruszewska A.
      Mol. Cell. Biol. 12:402-412(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
    2. Boguta M.
      Submitted (JUN-1994) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION.
    3. "A 43.5 kb segment of yeast chromosome XIV, which contains MFA2, MEP2, CAP/SRV2, NAM9, FKB1/FPR1/RBP1, MOM22 and CPT1, predicts an adenosine deaminase gene and 14 new open reading frames."
      Mallet L., Bussereau F., Jacquet M.
      Yeast 11:1195-1209(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    6. "The NAM9-1 suppressor mutation in a nuclear gene encoding ribosomal mitochondrial protein of Saccharomyces cerevisiae."
      Dmochowska A., Konopinska A., Krzymowska M., Szczesniak B., Boguta M.
      Gene 162:81-85(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF SER-82.
    7. "The single amino acid changes in the yeast mitochondrial S4 ribosomal protein cause temperature-sensitive defect in the accumulation of mitochondrial 15S rRNA."
      Biswas T.K., Getz G.S.
      Biochemistry 38:13042-13054(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT, SUBCELLULAR LOCATION, MUTAGENESIS OF LEU-109; ARG-111; PRO-424 AND PRO-438.
    8. "Identification of 12 new yeast mitochondrial ribosomal proteins including 6 that have no prokaryotic homologues."
      Saveanu C., Fromont-Racine M., Harington A., Ricard F., Namane A., Jacquier A.
      J. Biol. Chem. 276:15861-15867(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE MITOCHONDRIAL RIBOSOMAL SMALL COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
    9. "Tag-mediated isolation of yeast mitochondrial ribosome and mass spectrometric identification of its new components."
      Gan X., Kitakawa M., Yoshino K., Oshiro N., Yonezawa K., Isono K.
      Eur. J. Biochem. 269:5203-5214(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE MITOCHONDRIAL RIBOSOMAL SMALL COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
    10. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    11. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    12. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Strain: ATCC 76625 / YPH499.

    Entry informationi

    Entry nameiNAM9_YEAST
    AccessioniPrimary (citable) accession number: P27929
    Secondary accession number(s): D6W145
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 123 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 3270 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Ribosomal proteins
      Ribosomal proteins families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3