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P27929 (NAM9_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
37S ribosomal protein NAM9, mitochondrial
Alternative name(s):
Nuclear accommodation of mitochondria protein 9
Gene names
Name:NAM9
Synonyms:MNA6
Ordered Locus Names:YNL137C
ORF Names:N1211, N1840
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length486 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Subunit structure

Component of the mitochondrial small ribosomal subunit. Mature mitochondrial ribosomes consist of a small (37S) and a large (54S) subunit. The 37S subunit contains at least 33 different proteins and 1 molecule of RNA (15S). The 54S subunit contains at least 45 different proteins and 1 molecule of RNA (21S). Ref.7 Ref.8 Ref.9

Subcellular location

Mitochondrion Ref.7 Ref.10 Ref.12.

Miscellaneous

Present with 3270 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the ribosomal protein S4P family.

Contains 1 S4 RNA-binding domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3434Mitochondrion Potential
Chain35 – 48645237S ribosomal protein NAM9, mitochondrial
PRO_0000030639

Regions

Domain103 – 17270S4 RNA-binding

Experimental info

Mutagenesis821S → L in NAM9-1; suppressor for ocher mutations in mitochondrial DNA, possibly through decreasing the fidelity of translation. Ref.6
Mutagenesis1091L → F in MNA6-3; causes temperature-dependent loss of the 15S rRNA. Ref.7
Mutagenesis1111R → K in MNA6-1; causes temperature-dependent loss of the 15S rRNA. Ref.7
Mutagenesis4241P → L in MNA6-4; causes temperature-dependent loss of the 15S rRNA. Ref.7
Mutagenesis4381P → L in MNA6-2; causes temperature-dependent loss of the 15S rRNA. Ref.7

Sequences

Sequence LengthMass (Da)Tools
P27929 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: 4A5CB85B354955D1

FASTA48656,356
        10         20         30         40         50         60 
MPRKANLLKS LARGRVRTSF NKYNLFNLYK KGGVDLKSKS LYQQKWTAKQ ETRAYHGEHL 

        70         80         90        100        110        120 
TEKRWQTVFK PKLDSVAQLD ASLRGGEIKE TPFLLQTFAV LEKRLDFALF RAMFASSVRQ 

       130        140        150        160        170        180 
ARQFILHGNV RVNGVKIKHP SYTLKPGDMF SVKPDKVLEA LGAKKPSFQE ALKIDKTQIV 

       190        200        210        220        230        240 
LWNKYVKEAK TEPKEVWEKK LENFEKMSDS NPKKLQFQEF LRQYNKNLES QQYNALKGCT 

       250        260        270        280        290        300 
QEGILRKLLN VEKEIGKSNN EPLSIDELKQ GLPEIQDSQL LESLNNAYQE FFKSGEIRRE 

       310        320        330        340        350        360 
IISKCQPDEL ISLATEMMNP NETTKKELSD GAKSALRSGK RIIAESVKLW TKNITDHFKT 

       370        380        390        400        410        420 
RMSDISDGSL TFDPKWAKNL KYHDPIKLSE LEGDEPKARK LINLPWQKNY VYGRQDPKKP 

       430        440        450        460        470        480 
FFTPWKPRPF LSPFAILPHH LEISFKTCHA VYLRDPVARP GQSEVISPFD VPVHERAYMY 


YLRNGK 

« Hide

References

« Hide 'large scale' references
[1]"NAM9 nuclear suppressor of mitochondrial ochre mutations in Saccharomyces cerevisiae codes for a protein homologous to S4 ribosomal proteins from chloroplasts, bacteria, and eucaryotes."
Boguta M., Dmochowska A., Borsuk P., Wrobel K., Gargouri A., Lazowska J., Slonimski P.P., Szczesniak B., Kruszewska A.
Mol. Cell. Biol. 12:402-412(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
[2]Boguta M.
Submitted (JUN-1994) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION.
[3]"A 43.5 kb segment of yeast chromosome XIV, which contains MFA2, MEP2, CAP/SRV2, NAM9, FKB1/FPR1/RBP1, MOM22 and CPT1, predicts an adenosine deaminase gene and 14 new open reading frames."
Mallet L., Bussereau F., Jacquet M.
Yeast 11:1195-1209(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[6]"The NAM9-1 suppressor mutation in a nuclear gene encoding ribosomal mitochondrial protein of Saccharomyces cerevisiae."
Dmochowska A., Konopinska A., Krzymowska M., Szczesniak B., Boguta M.
Gene 162:81-85(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF SER-82.
[7]"The single amino acid changes in the yeast mitochondrial S4 ribosomal protein cause temperature-sensitive defect in the accumulation of mitochondrial 15S rRNA."
Biswas T.K., Getz G.S.
Biochemistry 38:13042-13054(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT, SUBCELLULAR LOCATION, MUTAGENESIS OF LEU-109; ARG-111; PRO-424 AND PRO-438.
[8]"Identification of 12 new yeast mitochondrial ribosomal proteins including 6 that have no prokaryotic homologues."
Saveanu C., Fromont-Racine M., Harington A., Ricard F., Namane A., Jacquier A.
J. Biol. Chem. 276:15861-15867(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE MITOCHONDRIAL RIBOSOMAL SMALL COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
[9]"Tag-mediated isolation of yeast mitochondrial ribosome and mass spectrometric identification of its new components."
Gan X., Kitakawa M., Yoshino K., Oshiro N., Yonezawa K., Isono K.
Eur. J. Biochem. 269:5203-5214(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE MITOCHONDRIAL RIBOSOMAL SMALL COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
[10]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[11]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[12]"The proteome of Saccharomyces cerevisiae mitochondria."
Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C.
Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Strain: ATCC 76625 / YPH499.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M60730 Unassigned DNA. Translation: AAA19439.1.
Z46843 Genomic DNA. Translation: CAA86888.1.
Z71413 Genomic DNA. Translation: CAA96019.1.
BK006947 Genomic DNA. Translation: DAA10411.1.
PIRS55146.
RefSeqNP_014262.3. NM_001182975.3.

3D structure databases

ProteinModelPortalP27929.
SMRP27929. Positions 42-153.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35689. 44 interactions.
DIPDIP-6702N.
IntActP27929. 32 interactions.
MINTMINT-652493.
STRING4932.YNL137C.

Proteomic databases

MaxQBP27929.
PaxDbP27929.
PeptideAtlasP27929.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNL137C; YNL137C; YNL137C.
GeneID855585.
KEGGsce:YNL137C.

Organism-specific databases

CYGDYNL137c.
SGDS000005081. NAM9.

Phylogenomic databases

eggNOGCOG0522.
HOGENOMHOG000209594.
OMAFRAMFAS.
OrthoDBEOG7HB5K7.

Enzyme and pathway databases

BioCycYEAST:G3O-33156-MONOMER.

Gene expression databases

GenevestigatorP27929.

Family and domain databases

Gene3D3.10.290.10. 1 hit.
InterProIPR022801. Ribosomal_S4/S9.
IPR018079. Ribosomal_S4_CS.
IPR002942. S4_RNA-bd.
[Graphical view]
PANTHERPTHR11831. PTHR11831. 1 hit.
PfamPF01479. S4. 1 hit.
[Graphical view]
SMARTSM00363. S4. 1 hit.
[Graphical view]
PROSITEPS00632. RIBOSOMAL_S4. 1 hit.
PS50889. S4. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio979718.

Entry information

Entry nameNAM9_YEAST
AccessionPrimary (citable) accession number: P27929
Secondary accession number(s): D6W145
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: February 1, 1996
Last modified: May 14, 2014
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Ribosomal proteins

Ribosomal proteins families and list of entries