P27929 (NAM9_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
37S ribosomal protein NAM9, mitochondrial

Alternative name(s):
Nuclear accommodation of mitochondria protein 9

Gene names

Name:	NAM9
Synonyms:	MNA6
Ordered Locus Names:	YNL137C
ORF Names:	N1211, N1840

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	486 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Component of the mitochondrial small ribosomal subunit. Mature mitochondrial ribosomes consist of a small (37S) and a large (54S) subunit. The 37S subunit contains at least 33 different proteins and 1 molecule of RNA (15S). The 54S subunit contains at least 45 different proteins and 1 molecule of RNA (21S). Ref.7 Ref.8 Ref.9
Subcellular location	Mitochondrion Ref.7 Ref.10 Ref.12.
Miscellaneous	Present with 3270 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the ribosomal protein S4P family. Contains 1 S4 RNA-binding domain.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	RNA-binding rRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mitochondrial translation Inferred by curator Ref.8. Source: SGD
Cellular_component	mitochondrial small ribosomal subunit Inferred from physical interaction Ref.8. Source: SGD
Molecular_function	rRNA binding Inferred from electronic annotation. Source: UniProtKB-KW structural constituent of ribosome Inferred from physical interaction Ref.8. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 34	34	Mitochondrion Potential
	Chain	35 – 486	452	37S ribosomal protein NAM9, mitochondrial		PRO_0000030639
Regions
	Domain	103 – 172	70	S4 RNA-binding
Experimental info
	Mutagenesis	82	1	S → L in NAM9-1; suppressor for ocher mutations in mitochondrial DNA, possibly through decreasing the fidelity of translation. Ref.6
	Mutagenesis	109	1	L → F in MNA6-3; causes temperature-dependent loss of the 15S rRNA. Ref.7
	Mutagenesis	111	1	R → K in MNA6-1; causes temperature-dependent loss of the 15S rRNA. Ref.7
	Mutagenesis	424	1	P → L in MNA6-4; causes temperature-dependent loss of the 15S rRNA. Ref.7
	Mutagenesis	438	1	P → L in MNA6-2; causes temperature-dependent loss of the 15S rRNA. Ref.7

Sequences

	Sequence		Length	Mass (Da)
	P27929 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: 4A5CB85B354955D1	FASTA	486	56,356
	10 20 30 40 50 60 MPRKANLLKS LARGRVRTSF NKYNLFNLYK KGGVDLKSKS LYQQKWTAKQ ETRAYHGEHL 70 80 90 100 110 120 TEKRWQTVFK PKLDSVAQLD ASLRGGEIKE TPFLLQTFAV LEKRLDFALF RAMFASSVRQ 130 140 150 160 170 180 ARQFILHGNV RVNGVKIKHP SYTLKPGDMF SVKPDKVLEA LGAKKPSFQE ALKIDKTQIV 190 200 210 220 230 240 LWNKYVKEAK TEPKEVWEKK LENFEKMSDS NPKKLQFQEF LRQYNKNLES QQYNALKGCT 250 260 270 280 290 300 QEGILRKLLN VEKEIGKSNN EPLSIDELKQ GLPEIQDSQL LESLNNAYQE FFKSGEIRRE 310 320 330 340 350 360 IISKCQPDEL ISLATEMMNP NETTKKELSD GAKSALRSGK RIIAESVKLW TKNITDHFKT 370 380 390 400 410 420 RMSDISDGSL TFDPKWAKNL KYHDPIKLSE LEGDEPKARK LINLPWQKNY VYGRQDPKKP 430 440 450 460 470 480 FFTPWKPRPF LSPFAILPHH LEISFKTCHA VYLRDPVARP GQSEVISPFD VPVHERAYMY YLRNGK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"NAM9 nuclear suppressor of mitochondrial ochre mutations in Saccharomyces cerevisiae codes for a protein homologous to S4 ribosomal proteins from chloroplasts, bacteria, and eucaryotes." Boguta M., Dmochowska A., Borsuk P., Wrobel K., Gargouri A., Lazowska J., Slonimski P.P., Szczesniak B., Kruszewska A. Mol. Cell. Biol. 12:402-412(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	Boguta M. Submitted (JUN-1994) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"A 43.5 kb segment of yeast chromosome XIV, which contains MFA2, MEP2, CAP/SRV2, NAM9, FKB1/FPR1/RBP1, MOM22 and CPT1, predicts an adenosine deaminase gene and 14 new open reading frames." Mallet L., Bussereau F., Jacquet M. Yeast 11:1195-1209(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[5]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[6]	"The NAM9-1 suppressor mutation in a nuclear gene encoding ribosomal mitochondrial protein of Saccharomyces cerevisiae." Dmochowska A., Konopinska A., Krzymowska M., Szczesniak B., Boguta M. Gene 162:81-85(1995) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF SER-82.
[7]	"The single amino acid changes in the yeast mitochondrial S4 ribosomal protein cause temperature-sensitive defect in the accumulation of mitochondrial 15S rRNA." Biswas T.K., Getz G.S. Biochemistry 38:13042-13054(1999) [PubMed] [Europe PMC] [Abstract] Cited for: SUBUNIT, SUBCELLULAR LOCATION, MUTAGENESIS OF LEU-109; ARG-111; PRO-424 AND PRO-438.
[8]	"Identification of 12 new yeast mitochondrial ribosomal proteins including 6 that have no prokaryotic homologues." Saveanu C., Fromont-Racine M., Harington A., Ricard F., Namane A., Jacquier A. J. Biol. Chem. 276:15861-15867(2001) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE MITOCHONDRIAL RIBOSOMAL SMALL COMPLEX, MASS SPECTROMETRY.
[9]	"Tag-mediated isolation of yeast mitochondrial ribosome and mass spectrometric identification of its new components." Gan X., Kitakawa M., Yoshino K., Oshiro N., Yonezawa K., Isono K. Eur. J. Biochem. 269:5203-5214(2002) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE MITOCHONDRIAL RIBOSOMAL SMALL COMPLEX, MASS SPECTROMETRY.
[10]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[11]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[12]	"The proteome of Saccharomyces cerevisiae mitochondria." Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C. Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Strain: ATCC 76625 / YPH499.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M60730 Unassigned DNA. Translation: AAA19439.1. Z46843 Genomic DNA. Translation: CAA86888.1. Z71413 Genomic DNA. Translation: CAA96019.1. BK006947 Genomic DNA. Translation: DAA10411.1.
PIR	S55146.
RefSeq	NP_014262.3. NM_001182975.3.
3D structure databases
ProteinModelPortal	P27929.
SMR	P27929. Positions 42-153.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6702N.
IntAct	P27929. 32 interactions.
MINT	MINT-652493.
STRING	4932.YNL137C.
Proteomic databases
PaxDb	P27929.
PeptideAtlas	P27929.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL137C; YNL137C; YNL137C.
GeneID	855585.
KEGG	sce:YNL137C.
Organism-specific databases
CYGD	YNL137c.
SGD	S000005081. NAM9.
Phylogenomic databases
eggNOG	COG0522.
HOGENOM	HOG000209594.
OMA	TRAYHGE.
OrthoDB	EOG43JGDB.
Enzyme and pathway databases
BioCyc	YEAST:G3O-33156-MONOMER.
Gene expression databases
Genevestigator	P27929.
GermOnline	YNL137C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.10.290.10. 1 hit.
InterPro	IPR022801. Ribosomal_S4/S9. IPR018079. Ribosomal_S4_CS. IPR002942. S4_RNA-bd. [Graphical view]
PANTHER	PTHR11831. PTHR11831. 1 hit.
Pfam	PF01479. S4. 1 hit. [Graphical view]
SMART	SM00363. S4. 1 hit. [Graphical view]
PROSITE	PS00632. RIBOSOMAL_S4. 1 hit. PS50889. S4. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	979718.

Entry information

Entry name

NAM9_YEAST

Accession

Primary (citable) accession number: P27929
Secondary accession number(s): D6W145

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	February 1, 1996
Last modified:	May 29, 2013
This is version 114 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program