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Protein

37S ribosomal protein NAM9, mitochondrial

Gene

NAM9

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.2 Publications

Miscellaneous

Present with 3270 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • rRNA binding Source: GO_Central
  • structural constituent of ribosome Source: SGD

GO - Biological processi

  • mitochondrial translation Source: SGD
  • positive regulation of translational fidelity Source: GO_Central

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein, RNA-binding, rRNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33156-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
37S ribosomal protein NAM9, mitochondrial
Alternative name(s):
Mitochondrial small ribosomal subunit protein uS4m1 Publication
Nuclear accommodation of mitochondria protein 9
Gene namesi
Name:NAM9
Synonyms:MNA6
Ordered Locus Names:YNL137C
ORF Names:N1211, N1840
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL137C
SGDiS000005081 NAM9

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi82S → L in NAM9-1; suppressor for ocher mutations in mitochondrial DNA, possibly through decreasing the fidelity of translation. 1 Publication1
Mutagenesisi109L → F in MNA6-3; causes temperature-dependent loss of the 15S rRNA. 1 Publication1
Mutagenesisi111R → K in MNA6-1; causes temperature-dependent loss of the 15S rRNA. 1 Publication1
Mutagenesisi424P → L in MNA6-4; causes temperature-dependent loss of the 15S rRNA. 1 Publication1
Mutagenesisi438P → L in MNA6-2; causes temperature-dependent loss of the 15S rRNA. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000306391 – 48637S ribosomal protein NAM9, mitochondrialAdd BLAST486

Proteomic databases

MaxQBiP27929
PaxDbiP27929
PRIDEiP27929

PTM databases

iPTMnetiP27929

Interactioni

Subunit structurei

Component of the mitochondrial small ribosomal subunit (mt-SSU). Mature yeast 74S mitochondrial ribosomes consist of a small (37S) and a large (54S) subunit. The 37S small subunit contains a 15S ribosomal RNA (15S mt-rRNA) and 34 different proteins. The 54S large subunit contains a 21S rRNA (21S mt-rRNA) and 46 different proteins. uS3m, uS4m and uS5m form the narrow entry site of the mRNA channel.4 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi35689, 44 interactors
DIPiDIP-6702N
IntActiP27929, 49 interactors
MINTiP27929
STRINGi4932.YNL137C

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5MRCelectron microscopy3.25DD1-486[»]
5MREelectron microscopy3.75DD1-486[»]
5MRFelectron microscopy4.97DD1-486[»]
ProteinModelPortaliP27929
SMRiP27929
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini103 – 172S4 RNA-bindingPROSITE-ProRule annotationAdd BLAST70

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000209594
InParanoidiP27929
OMAiTRAYHGE
OrthoDBiEOG092C56BX

Family and domain databases

CDDicd00165 S4, 1 hit
Gene3Di3.10.290.10, 1 hit
InterProiView protein in InterPro
IPR022801 Ribosomal_S4/S9
IPR005709 Ribosomal_S4_bac-type
IPR018079 Ribosomal_S4_CS
IPR002942 S4_RNA-bd
IPR036986 S4_RNA-bd_sf
PANTHERiPTHR11831 PTHR11831, 1 hit
PTHR11831:SF4 PTHR11831:SF4, 1 hit
PfamiView protein in Pfam
PF01479 S4, 1 hit
SMARTiView protein in SMART
SM00363 S4, 1 hit
PROSITEiView protein in PROSITE
PS00632 RIBOSOMAL_S4, 1 hit
PS50889 S4, 1 hit

Sequencei

Sequence statusi: Complete.

P27929-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRKANLLKS LARGRVRTSF NKYNLFNLYK KGGVDLKSKS LYQQKWTAKQ
60 70 80 90 100
ETRAYHGEHL TEKRWQTVFK PKLDSVAQLD ASLRGGEIKE TPFLLQTFAV
110 120 130 140 150
LEKRLDFALF RAMFASSVRQ ARQFILHGNV RVNGVKIKHP SYTLKPGDMF
160 170 180 190 200
SVKPDKVLEA LGAKKPSFQE ALKIDKTQIV LWNKYVKEAK TEPKEVWEKK
210 220 230 240 250
LENFEKMSDS NPKKLQFQEF LRQYNKNLES QQYNALKGCT QEGILRKLLN
260 270 280 290 300
VEKEIGKSNN EPLSIDELKQ GLPEIQDSQL LESLNNAYQE FFKSGEIRRE
310 320 330 340 350
IISKCQPDEL ISLATEMMNP NETTKKELSD GAKSALRSGK RIIAESVKLW
360 370 380 390 400
TKNITDHFKT RMSDISDGSL TFDPKWAKNL KYHDPIKLSE LEGDEPKARK
410 420 430 440 450
LINLPWQKNY VYGRQDPKKP FFTPWKPRPF LSPFAILPHH LEISFKTCHA
460 470 480
VYLRDPVARP GQSEVISPFD VPVHERAYMY YLRNGK
Length:486
Mass (Da):56,356
Last modified:February 1, 1996 - v2
Checksum:i4A5CB85B354955D1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60730 Unassigned DNA Translation: AAA19439.1
Z46843 Genomic DNA Translation: CAA86888.1
Z71413 Genomic DNA Translation: CAA96019.1
BK006947 Genomic DNA Translation: DAA10411.1
PIRiS55146
RefSeqiNP_014262.3, NM_001182975.3

Genome annotation databases

EnsemblFungiiYNL137C; YNL137C; YNL137C
GeneIDi855585
KEGGisce:YNL137C

Similar proteinsi

Entry informationi

Entry nameiNAM9_YEAST
AccessioniPrimary (citable) accession number: P27929
Secondary accession number(s): D6W145
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: February 1, 1996
Last modified: March 28, 2018
This is version 153 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health