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P27926 (HXK3_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hexokinase-3

EC=2.7.1.1
Alternative name(s):
Hexokinase type III
Short name=HK III
Gene names
Name:Hk3
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length924 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + D-hexose = ADP + D-hexose 6-phosphate.

Enzyme regulation

Hexokinase is an allosteric enzyme inhibited by its product Glc-6-P.

Pathway

Carbohydrate metabolism; hexose metabolism.

Subunit structure

Monomer.

Domain

The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus.

Miscellaneous

In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase).

Sequence similarities

Belongs to the hexokinase family.

Contains 2 hexokinase type-1 domains.

Contains 2 hexokinase type-2 domains.

Ontologies

Keywords
   Biological processGlycolysis
   DomainRepeat
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termAllosteric enzyme
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate phosphorylation

Inferred from direct assay PubMed 15138155. Source: GOC

cellular glucose homeostasis

Inferred from Biological aspect of Ancestor. Source: RefGenome

glucose 6-phosphate metabolic process

Inferred from Biological aspect of Ancestor. Source: GOC

glycolytic process

Inferred from Biological aspect of Ancestor. Source: RefGenome

hexose metabolic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytosol

Inferred from Biological aspect of Ancestor. Source: RefGenome

mitochondrion

Inferred from electronic annotation. Source: Ensembl

protein complex

Inferred from direct assay PubMed 11068878. Source: RGD

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

enzyme binding

Inferred from physical interaction PubMed 11068878. Source: RGD

fructokinase activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

glucokinase activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

hexokinase activity

Inferred from direct assay PubMed 15138155. Source: RGD

hormone binding

Inferred from physical interaction PubMed 11068878. Source: RGD

mannokinase activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

protein binding

Inferred from physical interaction PubMed 11068878. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 924924Hexokinase-3
PRO_0000197592

Regions

Domain33 – 231199Hexokinase type-1 1
Domain237 – 475239Hexokinase type-2 1
Domain480 – 676197Hexokinase type-1 2
Domain679 – 917239Hexokinase type-2 2
Nucleotide binding95 – 1006ATP Potential
Nucleotide binding542 – 5476ATP Potential
Region1 – 488488Regulatory
Region162 – 18827Glucose-binding Potential
Region489 – 924436Catalytic
Region604 – 63027Glucose-binding Potential

Experimental info

Sequence conflict9131R → A in AAI00649. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P27926 [UniParc].

Last modified August 1, 1992. Version 1.
Checksum: 0162C1591AF040AC

FASTA924100,254
        10         20         30         40         50         60 
MAAIEPSGLH PGERDSSCPQ EGIPRPSGSL ELAQEYLQQF KVTMTQLQQI QASLLCSMEQ 

        70         80         90        100        110        120 
ALKGQDSPAP SVRMLPTYVR STPHGTEQGD FLVLELGATG ASLRVLWVTL TGTKEHSVET 

       130        140        150        160        170        180 
RSQEFVIPQE VILGAGQQLF DFAARCLSEF LDAYPVENQG LKLGFNFSFP CHQTGLDKST 

       190        200        210        220        230        240 
LISWTKGFRC SGVEGQDVVQ LLRDAIQRQG TYNIDVVAMV NDTVGTMMGC ELGTRPCEVG 

       250        260        270        280        290        300 
LIVDTGTNAC YMEEARHVAA LDEDRGRTCV SIEWGSFYDE EALGPVLTTF DDALDHESLV 

       310        320        330        340        350        360 
PGAQRFEKMI GGLYLGELVR LVLVHLSQHG VLFGGCASPA LLSQNSILLE HVAKMEDPAT 

       370        380        390        400        410        420 
GIAHVHTVLQ GLGLSPQASD AELVQRVCMA VCTRAAQLCA SALAAVLSRL QHSREQQTLH 

       430        440        450        460        470        480 
VAVATGGRVF EWHPRFLCIL KETVMLLAPE CDVSFIPSVD GGGRGVAMVT AVAARLATHR 

       490        500        510        520        530        540 
RILEETLAPF QLSLEQLTAV QAQMREAMIR GLQGESSSLR MLPTYVRATP DGSERGDFLA 

       550        560        570        580        590        600 
LDLGGTNFRV LLVRVAEGSV QITNQVYSIP EYVAQGSGQK LFDHIVDCIV DFQKRQGLSG 

       610        620        630        640        650        660 
QSLPLGFTFS FPCKQLGLDQ GILLNWTKGF NASGCEGQDV VYLLREAIRR RQAVELNVVA 

       670        680        690        700        710        720 
IVNDTVGTMM SCGYDDPCCE MGLIVGTGTN ACYMEELRNV ASVPGDSGHM CINMEWGAFG 

       730        740        750        760        770        780 
DDGSLSMLGT CFDASVDQAS INPGKQRFEK MISGMYLGEI VRHILLHLTS LGVLFRGQKT 

       790        800        810        820        830        840 
QCLQTRDIFK TKFLSEIESD SLALRQVRAI LEDLGLTLTS DDALMVLEVC QAVSRRAAQL 

       850        860        870        880        890        900 
CGAGVAAVVE KIRENRGLQE LTVSVGVDGT LYKLHPHFSR LVSVTVRKLA PQCTVTFLQS 

       910        920 
EDGSGKGAAL VTRVACRLTQ MACV 

« Hide

References

« Hide 'large scale' references
[1]"Complete amino acid sequence of the type III isozyme of rat hexokinase, deduced from the cloned cDNA."
Schwab D.A., Wilson J.E.
Arch. Biochem. Biophys. 285:365-370(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.
[2]White J.A.
Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Prostate.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U73859 mRNA. Translation: AAB18253.1.
BC100648 mRNA. Translation: AAI00649.1.
PIRS13913.
RefSeqNP_071515.1. NM_022179.2.
XP_006253664.1. XM_006253602.1.
XP_006253665.1. XM_006253603.1.
UniGeneRn.162179.

3D structure databases

ProteinModelPortalP27926.
SMRP27926. Positions 29-918.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid247137. 10 interactions.
STRING10116.ENSRNOP00000031587.

Chemistry

ChEMBLCHEMBL3632.

PTM databases

PhosphoSiteP27926.

Proteomic databases

PaxDbP27926.
PRIDEP27926.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000031935; ENSRNOP00000031587; ENSRNOG00000026235.
GeneID25060.
KEGGrno:25060.
UCSCRGD:2798. rat.

Organism-specific databases

CTD3101.
RGD2798. Hk3.

Phylogenomic databases

eggNOGCOG5026.
GeneTreeENSGT00390000017159.
HOGENOMHOG000162671.
HOVERGENHBG005020.
InParanoidP27926.
KOK00844.
OrthoDBEOG7S21X5.
PhylomeDBP27926.
TreeFamTF314238.

Enzyme and pathway databases

SABIO-RKP27926.
UniPathwayUPA00242.

Gene expression databases

GenevestigatorP27926.

Family and domain databases

InterProIPR001312. Hexokinase.
IPR022673. Hexokinase_C.
IPR019807. Hexokinase_CS.
IPR022672. Hexokinase_N.
[Graphical view]
PANTHERPTHR19443. PTHR19443. 1 hit.
PfamPF00349. Hexokinase_1. 2 hits.
PF03727. Hexokinase_2. 2 hits.
[Graphical view]
PRINTSPR00475. HEXOKINASE.
PROSITEPS00378. HEXOKINASES. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio605280.

Entry information

Entry nameHXK3_RAT
AccessionPrimary (citable) accession number: P27926
Secondary accession number(s): Q497A1
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: June 11, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways