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P27926

- HXK3_RAT

UniProt

P27926 - HXK3_RAT

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Protein

Hexokinase-3

Gene

Hk3

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + D-hexose = ADP + D-hexose 6-phosphate.

Enzyme regulationi

Hexokinase is an allosteric enzyme inhibited by its product Glc-6-P.

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi95 – 1006ATPSequence Analysis
Nucleotide bindingi542 – 5476ATPSequence Analysis

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. enzyme binding Source: RGD
  3. fructokinase activity Source: RefGenome
  4. glucokinase activity Source: RefGenome
  5. hexokinase activity Source: RGD
  6. hormone binding Source: RGD
  7. mannokinase activity Source: RefGenome

GO - Biological processi

  1. carbohydrate phosphorylation Source: GOC
  2. cellular glucose homeostasis Source: RefGenome
  3. glucose 6-phosphate metabolic process Source: GOC
  4. glycolytic process Source: RefGenome
  5. hexose metabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_205960. Glucose transport.
SABIO-RKP27926.
UniPathwayiUPA00242.

Names & Taxonomyi

Protein namesi
Recommended name:
Hexokinase-3 (EC:2.7.1.1)
Alternative name(s):
Hexokinase type III
Short name:
HK III
Gene namesi
Name:Hk3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 17

Organism-specific databases

RGDi2798. Hk3.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: RefGenome
  2. mitochondrion Source: Ensembl
  3. protein complex Source: RGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 924924Hexokinase-3PRO_0000197592Add
BLAST

Proteomic databases

PaxDbiP27926.
PRIDEiP27926.

PTM databases

PhosphoSiteiP27926.

Expressioni

Gene expression databases

GenevestigatoriP27926.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi247137. 10 interactions.
STRINGi10116.ENSRNOP00000031587.

Structurei

3D structure databases

ProteinModelPortaliP27926.
SMRiP27926. Positions 29-918.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini33 – 231199Hexokinase type-1 1Add
BLAST
Domaini237 – 475239Hexokinase type-2 1Add
BLAST
Domaini480 – 676197Hexokinase type-1 2Add
BLAST
Domaini679 – 917239Hexokinase type-2 2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 488488RegulatoryAdd
BLAST
Regioni162 – 18827Glucose-bindingSequence AnalysisAdd
BLAST
Regioni489 – 924436CatalyticAdd
BLAST
Regioni604 – 63027Glucose-bindingSequence AnalysisAdd
BLAST

Domaini

The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus.

Sequence similaritiesi

Belongs to the hexokinase family.Curated
Contains 2 hexokinase type-1 domains.Curated
Contains 2 hexokinase type-2 domains.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG5026.
GeneTreeiENSGT00390000017159.
HOGENOMiHOG000162671.
HOVERGENiHBG005020.
InParanoidiP27926.
KOiK00844.
OrthoDBiEOG7S21X5.
PhylomeDBiP27926.
TreeFamiTF314238.

Family and domain databases

InterProiIPR001312. Hexokinase.
IPR022673. Hexokinase_C.
IPR019807. Hexokinase_CS.
IPR022672. Hexokinase_N.
[Graphical view]
PANTHERiPTHR19443. PTHR19443. 1 hit.
PfamiPF00349. Hexokinase_1. 2 hits.
PF03727. Hexokinase_2. 2 hits.
[Graphical view]
PRINTSiPR00475. HEXOKINASE.
PROSITEiPS00378. HEXOKINASES. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P27926-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAAIEPSGLH PGERDSSCPQ EGIPRPSGSL ELAQEYLQQF KVTMTQLQQI
60 70 80 90 100
QASLLCSMEQ ALKGQDSPAP SVRMLPTYVR STPHGTEQGD FLVLELGATG
110 120 130 140 150
ASLRVLWVTL TGTKEHSVET RSQEFVIPQE VILGAGQQLF DFAARCLSEF
160 170 180 190 200
LDAYPVENQG LKLGFNFSFP CHQTGLDKST LISWTKGFRC SGVEGQDVVQ
210 220 230 240 250
LLRDAIQRQG TYNIDVVAMV NDTVGTMMGC ELGTRPCEVG LIVDTGTNAC
260 270 280 290 300
YMEEARHVAA LDEDRGRTCV SIEWGSFYDE EALGPVLTTF DDALDHESLV
310 320 330 340 350
PGAQRFEKMI GGLYLGELVR LVLVHLSQHG VLFGGCASPA LLSQNSILLE
360 370 380 390 400
HVAKMEDPAT GIAHVHTVLQ GLGLSPQASD AELVQRVCMA VCTRAAQLCA
410 420 430 440 450
SALAAVLSRL QHSREQQTLH VAVATGGRVF EWHPRFLCIL KETVMLLAPE
460 470 480 490 500
CDVSFIPSVD GGGRGVAMVT AVAARLATHR RILEETLAPF QLSLEQLTAV
510 520 530 540 550
QAQMREAMIR GLQGESSSLR MLPTYVRATP DGSERGDFLA LDLGGTNFRV
560 570 580 590 600
LLVRVAEGSV QITNQVYSIP EYVAQGSGQK LFDHIVDCIV DFQKRQGLSG
610 620 630 640 650
QSLPLGFTFS FPCKQLGLDQ GILLNWTKGF NASGCEGQDV VYLLREAIRR
660 670 680 690 700
RQAVELNVVA IVNDTVGTMM SCGYDDPCCE MGLIVGTGTN ACYMEELRNV
710 720 730 740 750
ASVPGDSGHM CINMEWGAFG DDGSLSMLGT CFDASVDQAS INPGKQRFEK
760 770 780 790 800
MISGMYLGEI VRHILLHLTS LGVLFRGQKT QCLQTRDIFK TKFLSEIESD
810 820 830 840 850
SLALRQVRAI LEDLGLTLTS DDALMVLEVC QAVSRRAAQL CGAGVAAVVE
860 870 880 890 900
KIRENRGLQE LTVSVGVDGT LYKLHPHFSR LVSVTVRKLA PQCTVTFLQS
910 920
EDGSGKGAAL VTRVACRLTQ MACV
Length:924
Mass (Da):100,254
Last modified:August 1, 1992 - v1
Checksum:i0162C1591AF040AC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti913 – 9131R → A in AAI00649. (PubMed:15489334)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73859 mRNA. Translation: AAB18253.1.
BC100648 mRNA. Translation: AAI00649.1.
PIRiS13913.
RefSeqiNP_071515.1. NM_022179.2.
XP_006253664.1. XM_006253602.2.
XP_006253665.1. XM_006253603.2.
XP_008769706.1. XM_008771484.1.
UniGeneiRn.162179.

Genome annotation databases

EnsembliENSRNOT00000031935; ENSRNOP00000031587; ENSRNOG00000026235.
GeneIDi25060.
KEGGirno:25060.
UCSCiRGD:2798. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73859 mRNA. Translation: AAB18253.1 .
BC100648 mRNA. Translation: AAI00649.1 .
PIRi S13913.
RefSeqi NP_071515.1. NM_022179.2.
XP_006253664.1. XM_006253602.2.
XP_006253665.1. XM_006253603.2.
XP_008769706.1. XM_008771484.1.
UniGenei Rn.162179.

3D structure databases

ProteinModelPortali P27926.
SMRi P27926. Positions 29-918.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 247137. 10 interactions.
STRINGi 10116.ENSRNOP00000031587.

Chemistry

ChEMBLi CHEMBL3632.

PTM databases

PhosphoSitei P27926.

Proteomic databases

PaxDbi P27926.
PRIDEi P27926.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000031935 ; ENSRNOP00000031587 ; ENSRNOG00000026235 .
GeneIDi 25060.
KEGGi rno:25060.
UCSCi RGD:2798. rat.

Organism-specific databases

CTDi 3101.
RGDi 2798. Hk3.

Phylogenomic databases

eggNOGi COG5026.
GeneTreei ENSGT00390000017159.
HOGENOMi HOG000162671.
HOVERGENi HBG005020.
InParanoidi P27926.
KOi K00844.
OrthoDBi EOG7S21X5.
PhylomeDBi P27926.
TreeFami TF314238.

Enzyme and pathway databases

UniPathwayi UPA00242 .
Reactomei REACT_205960. Glucose transport.
SABIO-RK P27926.

Miscellaneous databases

NextBioi 605280.

Gene expression databases

Genevestigatori P27926.

Family and domain databases

InterProi IPR001312. Hexokinase.
IPR022673. Hexokinase_C.
IPR019807. Hexokinase_CS.
IPR022672. Hexokinase_N.
[Graphical view ]
PANTHERi PTHR19443. PTHR19443. 1 hit.
Pfami PF00349. Hexokinase_1. 2 hits.
PF03727. Hexokinase_2. 2 hits.
[Graphical view ]
PRINTSi PR00475. HEXOKINASE.
PROSITEi PS00378. HEXOKINASES. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete amino acid sequence of the type III isozyme of rat hexokinase, deduced from the cloned cDNA."
    Schwab D.A., Wilson J.E.
    Arch. Biochem. Biophys. 285:365-370(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  2. White J.A.
    Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Prostate.

Entry informationi

Entry nameiHXK3_RAT
AccessioniPrimary (citable) accession number: P27926
Secondary accession number(s): Q497A1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: November 26, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase).

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3