P27926 (HXK3_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hexokinase-3
EC=2.7.1.1

Alternative name(s):
Hexokinase type III
Short name=HK III

Gene names

Name:

Hk3

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	924 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + D-hexose = ADP + D-hexose 6-phosphate.
Enzyme regulation	Hexokinase is an allosteric enzyme inhibited by its product Glc-6-P.
Pathway	Carbohydrate metabolism; hexose metabolism.
Subunit structure	Monomer.
Domain	The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus.
Miscellaneous	In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase).
Sequence similarities	Belongs to the hexokinase family.

Ontologies

Keywords
Biological process	Glycolysis
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Allosteric enzyme Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	protein complex Inferred from direct assay. Source: RGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW enzyme binding Inferred from physical interaction. Source: RGD hexokinase activity Inferred from direct assay. Source: RGD hormone binding Inferred from physical interaction. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 924	924	Hexokinase-3		PRO_0000197592
Regions
	Nucleotide binding	95 – 100	6	ATP Potential
	Nucleotide binding	542 – 547	6	ATP Potential
	Region	1 – 488	488	Regulatory
	Region	162 – 188	27	Glucose-binding Potential
	Region	489 – 924	436	Catalytic
	Region	604 – 630	27	Glucose-binding Potential
Experimental info
	Sequence conflict	913	1	R → A in AAI00649. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P27926 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 0162C1591AF040AC	FASTA	924	100,254
	10 20 30 40 50 60 MAAIEPSGLH PGERDSSCPQ EGIPRPSGSL ELAQEYLQQF KVTMTQLQQI QASLLCSMEQ 70 80 90 100 110 120 ALKGQDSPAP SVRMLPTYVR STPHGTEQGD FLVLELGATG ASLRVLWVTL TGTKEHSVET 130 140 150 160 170 180 RSQEFVIPQE VILGAGQQLF DFAARCLSEF LDAYPVENQG LKLGFNFSFP CHQTGLDKST 190 200 210 220 230 240 LISWTKGFRC SGVEGQDVVQ LLRDAIQRQG TYNIDVVAMV NDTVGTMMGC ELGTRPCEVG 250 260 270 280 290 300 LIVDTGTNAC YMEEARHVAA LDEDRGRTCV SIEWGSFYDE EALGPVLTTF DDALDHESLV 310 320 330 340 350 360 PGAQRFEKMI GGLYLGELVR LVLVHLSQHG VLFGGCASPA LLSQNSILLE HVAKMEDPAT 370 380 390 400 410 420 GIAHVHTVLQ GLGLSPQASD AELVQRVCMA VCTRAAQLCA SALAAVLSRL QHSREQQTLH 430 440 450 460 470 480 VAVATGGRVF EWHPRFLCIL KETVMLLAPE CDVSFIPSVD GGGRGVAMVT AVAARLATHR 490 500 510 520 530 540 RILEETLAPF QLSLEQLTAV QAQMREAMIR GLQGESSSLR MLPTYVRATP DGSERGDFLA 550 560 570 580 590 600 LDLGGTNFRV LLVRVAEGSV QITNQVYSIP EYVAQGSGQK LFDHIVDCIV DFQKRQGLSG 610 620 630 640 650 660 QSLPLGFTFS FPCKQLGLDQ GILLNWTKGF NASGCEGQDV VYLLREAIRR RQAVELNVVA 670 680 690 700 710 720 IVNDTVGTMM SCGYDDPCCE MGLIVGTGTN ACYMEELRNV ASVPGDSGHM CINMEWGAFG 730 740 750 760 770 780 DDGSLSMLGT CFDASVDQAS INPGKQRFEK MISGMYLGEI VRHILLHLTS LGVLFRGQKT 790 800 810 820 830 840 QCLQTRDIFK TKFLSEIESD SLALRQVRAI LEDLGLTLTS DDALMVLEVC QAVSRRAAQL 850 860 870 880 890 900 CGAGVAAVVE KIRENRGLQE LTVSVGVDGT LYKLHPHFSR LVSVTVRKLA PQCTVTFLQS 910 920 EDGSGKGAAL VTRVACRLTQ MACV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete amino acid sequence of the type III isozyme of rat hexokinase, deduced from the cloned cDNA." Schwab D.A., Wilson J.E. Arch. Biochem. Biophys. 285:365-370(1991) [PubMed: 1897938] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	White J.A. Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Prostate.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U73859 mRNA. Translation: AAB18253.1. BC100648 mRNA. Translation: AAI00649.1.
IPI	IPI00201891.
PIR	S13913.
RefSeq	NP_071515.1. NM_022179.2.
UniGene	Rn.162179.
3D structure databases
ProteinModelPortal	P27926.
SMR	P27926. Positions 29-918.
ModBase	Search...
Protein-protein interaction databases
STRING	P27926.
PTM databases
PhosphoSite	P27926.
Proteomic databases
PRIDE	P27926.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000031935; ENSRNOP00000031587; ENSRNOG00000026235.
GeneID	25060.
KEGG	rno:25060.
UCSC	NM_022179. rat.
Organism-specific databases
CTD	3101.
RGD	2798. Hk3.
Phylogenomic databases
eggNOG	roNOG15124.
GeneTree	ENSGT00390000017159.
HOVERGEN	HBG005020.
InParanoid	P27926.
OrthoDB	EOG4R23T4.
PhylomeDB	P27926.
Gene expression databases
Genevestigator	P27926.
Family and domain databases
InterPro	IPR001312. Hexokinase. IPR022673. Hexokinase_C. IPR019807. Hexokinase_CS. IPR022672. Hexokinase_N. [Graphical view]
KO	K00844.
PANTHER	PTHR19443. Hexokinase. 1 hit.
Pfam	PF00349. Hexokinase_1. 2 hits. PF03727. Hexokinase_2. 2 hits. [Graphical view]
PRINTS	PR00475. HEXOKINASE.
PROSITE	PS00378. HEXOKINASES. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	605280.

Entry information

Entry name

HXK3_RAT

Accession

Primary (citable) accession number: P27926
Secondary accession number(s): Q497A1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	November 16, 2011
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program