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Protein

Hexokinase-3

Gene

Hk3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + D-hexose = ADP + D-hexose 6-phosphate.

Enzyme regulationi

Hexokinase is an allosteric enzyme inhibited by its product Glc-6-P.

Pathway:ihexose metabolism

This protein is involved in the pathway hexose metabolism, which is part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the pathway hexose metabolism and in Carbohydrate metabolism.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi95 – 1006ATPSequence Analysis
Nucleotide bindingi542 – 5476ATPSequence Analysis

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • enzyme binding Source: RGD
  • fructokinase activity Source: GO_Central
  • glucokinase activity Source: GO_Central
  • hexokinase activity Source: RGD
  • hormone binding Source: RGD
  • mannokinase activity Source: GO_Central

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_275920. Glucose transport.
REACT_342181. Glycolysis.
SABIO-RKP27926.
UniPathwayiUPA00242.

Names & Taxonomyi

Protein namesi
Recommended name:
Hexokinase-3 (EC:2.7.1.1)
Alternative name(s):
Hexokinase type III
Short name:
HK III
Gene namesi
Name:Hk3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2798. Hk3.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: GO_Central
  • protein complex Source: RGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 924924Hexokinase-3PRO_0000197592Add
BLAST

Proteomic databases

PaxDbiP27926.
PRIDEiP27926.

PTM databases

PhosphoSiteiP27926.

Expressioni

Gene expression databases

GenevisibleiP27926. RN.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi247137. 10 interactions.
STRINGi10116.ENSRNOP00000031587.

Structurei

3D structure databases

ProteinModelPortaliP27926.
SMRiP27926. Positions 29-918.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 471445Hexokinase 1PROSITE-ProRule annotationAdd
BLAST
Domaini477 – 913437Hexokinase 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 488488RegulatoryAdd
BLAST
Regioni84 – 220137Hexokinase small subdomain 1PROSITE-ProRule annotationAdd
BLAST
Regioni162 – 18827Glucose-bindingSequence AnalysisAdd
BLAST
Regioni221 – 460240Hexokinase large subdomain 1PROSITE-ProRule annotationAdd
BLAST
Regioni489 – 924436CatalyticAdd
BLAST
Regioni531 – 662132Hexokinase small subdomain 2PROSITE-ProRule annotationAdd
BLAST
Regioni604 – 63027Glucose-bindingSequence AnalysisAdd
BLAST
Regioni663 – 902240Hexokinase large subdomain 2PROSITE-ProRule annotationAdd
BLAST

Domaini

The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus.

Sequence similaritiesi

Belongs to the hexokinase family.PROSITE-ProRule annotationCurated
Contains 2 hexokinase domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG5026.
HOGENOMiHOG000162671.
HOVERGENiHBG005020.
InParanoidiP27926.
KOiK00844.
OrthoDBiEOG7S21X5.
PhylomeDBiP27926.
TreeFamiTF314238.

Family and domain databases

InterProiIPR001312. Hexokinase.
IPR019807. Hexokinase_BS.
IPR022673. Hexokinase_C.
IPR022672. Hexokinase_N.
[Graphical view]
PANTHERiPTHR19443. PTHR19443. 1 hit.
PfamiPF00349. Hexokinase_1. 2 hits.
PF03727. Hexokinase_2. 2 hits.
[Graphical view]
PROSITEiPS00378. HEXOKINASE_1. 2 hits.
PS51748. HEXOKINASE_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P27926-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAIEPSGLH PGERDSSCPQ EGIPRPSGSL ELAQEYLQQF KVTMTQLQQI
60 70 80 90 100
QASLLCSMEQ ALKGQDSPAP SVRMLPTYVR STPHGTEQGD FLVLELGATG
110 120 130 140 150
ASLRVLWVTL TGTKEHSVET RSQEFVIPQE VILGAGQQLF DFAARCLSEF
160 170 180 190 200
LDAYPVENQG LKLGFNFSFP CHQTGLDKST LISWTKGFRC SGVEGQDVVQ
210 220 230 240 250
LLRDAIQRQG TYNIDVVAMV NDTVGTMMGC ELGTRPCEVG LIVDTGTNAC
260 270 280 290 300
YMEEARHVAA LDEDRGRTCV SIEWGSFYDE EALGPVLTTF DDALDHESLV
310 320 330 340 350
PGAQRFEKMI GGLYLGELVR LVLVHLSQHG VLFGGCASPA LLSQNSILLE
360 370 380 390 400
HVAKMEDPAT GIAHVHTVLQ GLGLSPQASD AELVQRVCMA VCTRAAQLCA
410 420 430 440 450
SALAAVLSRL QHSREQQTLH VAVATGGRVF EWHPRFLCIL KETVMLLAPE
460 470 480 490 500
CDVSFIPSVD GGGRGVAMVT AVAARLATHR RILEETLAPF QLSLEQLTAV
510 520 530 540 550
QAQMREAMIR GLQGESSSLR MLPTYVRATP DGSERGDFLA LDLGGTNFRV
560 570 580 590 600
LLVRVAEGSV QITNQVYSIP EYVAQGSGQK LFDHIVDCIV DFQKRQGLSG
610 620 630 640 650
QSLPLGFTFS FPCKQLGLDQ GILLNWTKGF NASGCEGQDV VYLLREAIRR
660 670 680 690 700
RQAVELNVVA IVNDTVGTMM SCGYDDPCCE MGLIVGTGTN ACYMEELRNV
710 720 730 740 750
ASVPGDSGHM CINMEWGAFG DDGSLSMLGT CFDASVDQAS INPGKQRFEK
760 770 780 790 800
MISGMYLGEI VRHILLHLTS LGVLFRGQKT QCLQTRDIFK TKFLSEIESD
810 820 830 840 850
SLALRQVRAI LEDLGLTLTS DDALMVLEVC QAVSRRAAQL CGAGVAAVVE
860 870 880 890 900
KIRENRGLQE LTVSVGVDGT LYKLHPHFSR LVSVTVRKLA PQCTVTFLQS
910 920
EDGSGKGAAL VTRVACRLTQ MACV
Length:924
Mass (Da):100,254
Last modified:August 1, 1992 - v1
Checksum:i0162C1591AF040AC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti913 – 9131R → A in AAI00649 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73859 mRNA. Translation: AAB18253.1.
BC100648 mRNA. Translation: AAI00649.1.
PIRiS13913.
RefSeqiNP_071515.1. NM_022179.2.
XP_006253664.1. XM_006253602.2.
XP_006253665.1. XM_006253603.2.
XP_008769706.1. XM_008771484.1.
UniGeneiRn.162179.

Genome annotation databases

GeneIDi25060.
KEGGirno:25060.
UCSCiRGD:2798. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73859 mRNA. Translation: AAB18253.1.
BC100648 mRNA. Translation: AAI00649.1.
PIRiS13913.
RefSeqiNP_071515.1. NM_022179.2.
XP_006253664.1. XM_006253602.2.
XP_006253665.1. XM_006253603.2.
XP_008769706.1. XM_008771484.1.
UniGeneiRn.162179.

3D structure databases

ProteinModelPortaliP27926.
SMRiP27926. Positions 29-918.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi247137. 10 interactions.
STRINGi10116.ENSRNOP00000031587.

Chemistry

ChEMBLiCHEMBL3632.

PTM databases

PhosphoSiteiP27926.

Proteomic databases

PaxDbiP27926.
PRIDEiP27926.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi25060.
KEGGirno:25060.
UCSCiRGD:2798. rat.

Organism-specific databases

CTDi3101.
RGDi2798. Hk3.

Phylogenomic databases

eggNOGiCOG5026.
HOGENOMiHOG000162671.
HOVERGENiHBG005020.
InParanoidiP27926.
KOiK00844.
OrthoDBiEOG7S21X5.
PhylomeDBiP27926.
TreeFamiTF314238.

Enzyme and pathway databases

UniPathwayiUPA00242.
ReactomeiREACT_275920. Glucose transport.
REACT_342181. Glycolysis.
SABIO-RKP27926.

Miscellaneous databases

NextBioi605280.
PROiP27926.

Gene expression databases

GenevisibleiP27926. RN.

Family and domain databases

InterProiIPR001312. Hexokinase.
IPR019807. Hexokinase_BS.
IPR022673. Hexokinase_C.
IPR022672. Hexokinase_N.
[Graphical view]
PANTHERiPTHR19443. PTHR19443. 1 hit.
PfamiPF00349. Hexokinase_1. 2 hits.
PF03727. Hexokinase_2. 2 hits.
[Graphical view]
PROSITEiPS00378. HEXOKINASE_1. 2 hits.
PS51748. HEXOKINASE_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete amino acid sequence of the type III isozyme of rat hexokinase, deduced from the cloned cDNA."
    Schwab D.A., Wilson J.E.
    Arch. Biochem. Biophys. 285:365-370(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  2. White J.A.
    Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Prostate.

Entry informationi

Entry nameiHXK3_RAT
AccessioniPrimary (citable) accession number: P27926
Secondary accession number(s): Q497A1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: July 22, 2015
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase).

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.