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P27926

- HXK3_RAT

UniProt

P27926 - HXK3_RAT

Protein

Hexokinase-3

Gene

Hk3

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (01 Aug 1992)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + D-hexose = ADP + D-hexose 6-phosphate.

    Enzyme regulationi

    Hexokinase is an allosteric enzyme inhibited by its product Glc-6-P.

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi95 – 1006ATPSequence Analysis
    Nucleotide bindingi542 – 5476ATPSequence Analysis

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. enzyme binding Source: RGD
    3. fructokinase activity Source: RefGenome
    4. glucokinase activity Source: RefGenome
    5. hexokinase activity Source: RGD
    6. hormone binding Source: RGD
    7. mannokinase activity Source: RefGenome
    8. protein binding Source: RGD

    GO - Biological processi

    1. carbohydrate phosphorylation Source: GOC
    2. cellular glucose homeostasis Source: RefGenome
    3. glucose 6-phosphate metabolic process Source: GOC
    4. glycolytic process Source: RefGenome
    5. hexose metabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_205960. Glucose transport.
    SABIO-RKP27926.
    UniPathwayiUPA00242.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hexokinase-3 (EC:2.7.1.1)
    Alternative name(s):
    Hexokinase type III
    Short name:
    HK III
    Gene namesi
    Name:Hk3
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 17

    Organism-specific databases

    RGDi2798. Hk3.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: RefGenome
    2. mitochondrion Source: Ensembl
    3. protein complex Source: RGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 924924Hexokinase-3PRO_0000197592Add
    BLAST

    Proteomic databases

    PaxDbiP27926.
    PRIDEiP27926.

    PTM databases

    PhosphoSiteiP27926.

    Expressioni

    Gene expression databases

    GenevestigatoriP27926.

    Interactioni

    Subunit structurei

    Monomer.

    Protein-protein interaction databases

    BioGridi247137. 10 interactions.
    STRINGi10116.ENSRNOP00000031587.

    Structurei

    3D structure databases

    ProteinModelPortaliP27926.
    SMRiP27926. Positions 29-918.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini33 – 231199Hexokinase type-1 1Add
    BLAST
    Domaini237 – 475239Hexokinase type-2 1Add
    BLAST
    Domaini480 – 676197Hexokinase type-1 2Add
    BLAST
    Domaini679 – 917239Hexokinase type-2 2Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 488488RegulatoryAdd
    BLAST
    Regioni162 – 18827Glucose-bindingSequence AnalysisAdd
    BLAST
    Regioni489 – 924436CatalyticAdd
    BLAST
    Regioni604 – 63027Glucose-bindingSequence AnalysisAdd
    BLAST

    Domaini

    The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus.

    Sequence similaritiesi

    Belongs to the hexokinase family.Curated
    Contains 2 hexokinase type-1 domains.Curated
    Contains 2 hexokinase type-2 domains.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG5026.
    GeneTreeiENSGT00390000017159.
    HOGENOMiHOG000162671.
    HOVERGENiHBG005020.
    InParanoidiP27926.
    KOiK00844.
    OrthoDBiEOG7S21X5.
    PhylomeDBiP27926.
    TreeFamiTF314238.

    Family and domain databases

    InterProiIPR001312. Hexokinase.
    IPR022673. Hexokinase_C.
    IPR019807. Hexokinase_CS.
    IPR022672. Hexokinase_N.
    [Graphical view]
    PANTHERiPTHR19443. PTHR19443. 1 hit.
    PfamiPF00349. Hexokinase_1. 2 hits.
    PF03727. Hexokinase_2. 2 hits.
    [Graphical view]
    PRINTSiPR00475. HEXOKINASE.
    PROSITEiPS00378. HEXOKINASES. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P27926-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAIEPSGLH PGERDSSCPQ EGIPRPSGSL ELAQEYLQQF KVTMTQLQQI    50
    QASLLCSMEQ ALKGQDSPAP SVRMLPTYVR STPHGTEQGD FLVLELGATG 100
    ASLRVLWVTL TGTKEHSVET RSQEFVIPQE VILGAGQQLF DFAARCLSEF 150
    LDAYPVENQG LKLGFNFSFP CHQTGLDKST LISWTKGFRC SGVEGQDVVQ 200
    LLRDAIQRQG TYNIDVVAMV NDTVGTMMGC ELGTRPCEVG LIVDTGTNAC 250
    YMEEARHVAA LDEDRGRTCV SIEWGSFYDE EALGPVLTTF DDALDHESLV 300
    PGAQRFEKMI GGLYLGELVR LVLVHLSQHG VLFGGCASPA LLSQNSILLE 350
    HVAKMEDPAT GIAHVHTVLQ GLGLSPQASD AELVQRVCMA VCTRAAQLCA 400
    SALAAVLSRL QHSREQQTLH VAVATGGRVF EWHPRFLCIL KETVMLLAPE 450
    CDVSFIPSVD GGGRGVAMVT AVAARLATHR RILEETLAPF QLSLEQLTAV 500
    QAQMREAMIR GLQGESSSLR MLPTYVRATP DGSERGDFLA LDLGGTNFRV 550
    LLVRVAEGSV QITNQVYSIP EYVAQGSGQK LFDHIVDCIV DFQKRQGLSG 600
    QSLPLGFTFS FPCKQLGLDQ GILLNWTKGF NASGCEGQDV VYLLREAIRR 650
    RQAVELNVVA IVNDTVGTMM SCGYDDPCCE MGLIVGTGTN ACYMEELRNV 700
    ASVPGDSGHM CINMEWGAFG DDGSLSMLGT CFDASVDQAS INPGKQRFEK 750
    MISGMYLGEI VRHILLHLTS LGVLFRGQKT QCLQTRDIFK TKFLSEIESD 800
    SLALRQVRAI LEDLGLTLTS DDALMVLEVC QAVSRRAAQL CGAGVAAVVE 850
    KIRENRGLQE LTVSVGVDGT LYKLHPHFSR LVSVTVRKLA PQCTVTFLQS 900
    EDGSGKGAAL VTRVACRLTQ MACV 924
    Length:924
    Mass (Da):100,254
    Last modified:August 1, 1992 - v1
    Checksum:i0162C1591AF040AC
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti913 – 9131R → A in AAI00649. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U73859 mRNA. Translation: AAB18253.1.
    BC100648 mRNA. Translation: AAI00649.1.
    PIRiS13913.
    RefSeqiNP_071515.1. NM_022179.2.
    XP_006253664.1. XM_006253602.1.
    XP_006253665.1. XM_006253603.1.
    UniGeneiRn.162179.

    Genome annotation databases

    EnsembliENSRNOT00000031935; ENSRNOP00000031587; ENSRNOG00000026235.
    GeneIDi25060.
    KEGGirno:25060.
    UCSCiRGD:2798. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U73859 mRNA. Translation: AAB18253.1 .
    BC100648 mRNA. Translation: AAI00649.1 .
    PIRi S13913.
    RefSeqi NP_071515.1. NM_022179.2.
    XP_006253664.1. XM_006253602.1.
    XP_006253665.1. XM_006253603.1.
    UniGenei Rn.162179.

    3D structure databases

    ProteinModelPortali P27926.
    SMRi P27926. Positions 29-918.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 247137. 10 interactions.
    STRINGi 10116.ENSRNOP00000031587.

    Chemistry

    ChEMBLi CHEMBL3632.

    PTM databases

    PhosphoSitei P27926.

    Proteomic databases

    PaxDbi P27926.
    PRIDEi P27926.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000031935 ; ENSRNOP00000031587 ; ENSRNOG00000026235 .
    GeneIDi 25060.
    KEGGi rno:25060.
    UCSCi RGD:2798. rat.

    Organism-specific databases

    CTDi 3101.
    RGDi 2798. Hk3.

    Phylogenomic databases

    eggNOGi COG5026.
    GeneTreei ENSGT00390000017159.
    HOGENOMi HOG000162671.
    HOVERGENi HBG005020.
    InParanoidi P27926.
    KOi K00844.
    OrthoDBi EOG7S21X5.
    PhylomeDBi P27926.
    TreeFami TF314238.

    Enzyme and pathway databases

    UniPathwayi UPA00242 .
    Reactomei REACT_205960. Glucose transport.
    SABIO-RK P27926.

    Miscellaneous databases

    NextBioi 605280.

    Gene expression databases

    Genevestigatori P27926.

    Family and domain databases

    InterProi IPR001312. Hexokinase.
    IPR022673. Hexokinase_C.
    IPR019807. Hexokinase_CS.
    IPR022672. Hexokinase_N.
    [Graphical view ]
    PANTHERi PTHR19443. PTHR19443. 1 hit.
    Pfami PF00349. Hexokinase_1. 2 hits.
    PF03727. Hexokinase_2. 2 hits.
    [Graphical view ]
    PRINTSi PR00475. HEXOKINASE.
    PROSITEi PS00378. HEXOKINASES. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete amino acid sequence of the type III isozyme of rat hexokinase, deduced from the cloned cDNA."
      Schwab D.A., Wilson J.E.
      Arch. Biochem. Biophys. 285:365-370(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Liver.
    2. White J.A.
      Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Liver.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Prostate.

    Entry informationi

    Entry nameiHXK3_RAT
    AccessioniPrimary (citable) accession number: P27926
    Secondary accession number(s): Q497A1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: August 1, 1992
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase).

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3