Reviewed, UniProtKB/Swiss-Prot P27914 (POLG_DEN2T)

Names and origin

Protein names	Recommended name:     Genome polyprotein Cleaved into the following 5 chains:     1- Recommended name:             Envelope protein E     2- Recommended name:             Non-structural protein 1                 Short name=NS1     3- Recommended name:             Non-structural protein 2A                 Short name=NS2A     4- Recommended name:             Non-structural protein 2A-alpha                 Short name=NS2A-alpha     5- Recommended name:             Serine protease subunit NS3               EC=3.4.21.91         Alternative name(s):             Non-structural protein 3
Organism	Dengue virus type 2 (strain Tonga/EKB194/1974) (DENV-2)
Taxonomic identifier	11067 [NCBI]
Taxonomic lineage	Viruses › ssRNA positive-strand viruses, no DNA stage › Flaviviridae › Flavivirus › Dengue virus group
Virus host	Erythrocebus patas (Red guenon) (Cercopithecus patas) [TaxID: 9538] Homo sapiens (Human) [TaxID: 9606] Diceromyia [TaxID: 53539] Aedimorphus [TaxID: 53540] Stegomyia [TaxID: 53541]

Protein attributes

Sequence length	1683 AA.
Sequence status	Fragments.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala.
Subcellular location	Envelope protein E: Virion Potential. Host membrane; Multi-pass membrane protein Potential.
Post-translational modification	Specific enzymatic cleavages in vivo yield mature proteins By similarity.
Miscellaneous	The virion of this virus is a nucleocapsid covered by a lipoprotein envelope. The envelope contains two proteins: the protein M and glycoprotein E. The nucleocapsid is a complex of protein C and mRNA. In immature particles, there are 60 icosaedrally organized trimeric spikes on the surface. Each spike consists of three heterodimers of envelope protein M precursor (prM) and envelope protein E By similarity.
Sequence similarities	Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.

Ontologies

Keywords
Cellular component	Capsid protein Core protein Envelope protein Host membrane Membrane Virion
Domain	Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
PTM	Cleavage on pair of basic residues Disulfide bond Glycoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	viral genome replication Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell viral envelope Inferred from electronic annotation. Source: UniProtKB-KW viral nucleocapsid Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent helicase activity Inferred from electronic annotation. Source: InterPro RNA helicase activity Inferred from electronic annotation. Source: InterPro double-stranded RNA binding Inferred from electronic annotation. Source: InterPro structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 495

495

Envelope protein E By similarity

PRO_0000037980

Chain

496 – 847

352

Non-structural protein 1 By similarity

PRO_0000037981

Chain

848 – ›1064

›217

Non-structural protein 2A By similarity

PRO_0000308290

Chain

848 – 1035

188

Non-structural protein 2A-alpha By similarity

PRO_0000037982

Chain

1066 – 1683

618

Serine protease subunit NS3 By similarity

PRO_0000037983

Regions

Topological domain

7 – 445

439

Lumenal Potential

Transmembrane

446 – 466

21

Potential

Topological domain

467 – 472

6

Cytoplasmic Potential

Transmembrane

473 – 493

21

Potential

Topological domain

494 – 876

383

Lumenal Potential

Transmembrane

877 – 897

21

Potential

Topological domain

898 – ›1065

›168

Cytoplasmic Potential

Domain

1245 – 1401

157

Helicase ATP-binding

Domain

1411 – 1582

172

Helicase C-terminal

Nucleotide binding

1258 – 1265

8

ATP Potential

Motif

1349 – 1352

4

DEAH box

Sites

Active site

1116

1

Charge relay system; for serine protease NS3 activity By similarity

Active site

1140

1

Charge relay system; for serine protease NS3 activity By similarity

Active site

1200

1

Charge relay system; for serine protease NS3 activity By similarity

Site

495 – 496

2

Cleavage; by host signal peptidase By similarity

Site

847 – 848

2

Cleavage; by host By similarity

Site

1035 – 1036

2

Cleavage; by serine protease NS3 By similarity

Site

1065 – 1066

2

Cleavage; by serine protease NS3 By similarity

Amino acid modifications

Glycosylation

67

1

N-linked (GlcNAc...); by host Potential

Glycosylation

153

1

N-linked (GlcNAc...); by host Potential

Disulfide bond

3 ↔ 30

By similarity

Disulfide bond

60 ↔ 121

By similarity

Disulfide bond

74 ↔ 105

By similarity

Disulfide bond

92 ↔ 116

By similarity

Disulfide bond

185 ↔ 285

By similarity

Disulfide bond

302 ↔ 333

By similarity

Experimental info

Non-adjacent residues

1065 – 1066

2

Non-terminal residue

1

1

Non-terminal residue

1683

1

Secondary structure

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1683

Helix Strand Turn

Details...

Turn

2 – 5

4

Beta strand

7 – 13

7

Beta strand

20 – 24

5

Beta strand

30 – 34

5

Beta strand

36 – 38

3

Beta strand

41 – 50

10

Beta strand

55 – 72

18

Beta strand

75 – 77

3

Helix

83 – 85

3

Beta strand

90 – 100

11

Helix

101 – 103

3

Beta strand

109 – 128

20

Beta strand

135 – 143

9

Turn

148 – 152

5

Beta strand

159 – 164

6

Beta strand

171 – 175

5

Turn

176 – 178

3

Beta strand

179 – 186

8

Helix

193 – 195

3

Beta strand

196 – 201

6

Beta strand

204 – 209

6

Helix

210 – 214

5

Beta strand

220 – 222

3

Helix

234 – 237

4

Beta strand

238 – 241

4

Beta strand

249 – 252

4

Helix

257 – 263

7

Turn

264 – 266

3

Beta strand

273 – 276

4

Beta strand

284 – 288

5

Beta strand

306 – 310

5

Beta strand

320 – 326

7

Beta strand

332 – 334

3

Beta strand

337 – 340

4

Beta strand

346 – 353

8

Beta strand

365 – 370

6

Beta strand

373 – 381

9

Beta strand

383 – 385

3

Beta strand

387 – 393

7

Sequences

	Sequence		Length	Mass (Da)
	P27914-1 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 3B0438D96196BFC8	FASTA	1,683	187,440
	10 20 30 40 50 60 MRCIGISNRD FVEGVSGGSW VDIVLEHGSC VTTMAKNKPT LDFELIKTEA KQPATLRKYC 70 80 90 100 110 120 IEAKLTNTTT DSRCPTQGEP TLNEEQDKRF VCKHSMVDRG WGNGCGLFGK GGIVTCAMFT 130 140 150 160 170 180 CKKNMEGKIV QPENLEYTVV ITPHSGEEHA VGNDTGKHGK EVKITPQSSI TEAELTGYGT 190 200 210 220 230 240 VTMECSPRTG LDFNEMVLLQ MEDKAWLVHR QWFLDLPLPW LPGADTQGSN WIQKETLVTF 250 260 270 280 290 300 KNPHAKKQDV VVLGSQEGAM HTALTGATEI QMSSGNLLFT GHLKCRLRMD KLQLKGMSYS 310 320 330 340 350 360 MCTGKFKIVK EIAETQHGTI VIRVQYEGDG SPCKIPFEIM DLEKRHVLGR LITVNPIVTE 370 380 390 400 410 420 KDSPVNIEAE PPFGDSYIII GVEPGQLKLD WFKKGSSIGQ MFETTMRGAK RMAILGDTAW 430 440 450 460 470 480 DFGSLGGVFT SIGKALHQVF GAIYGAAFSG VSWTMKILIG VIITWIGMNS RSTSLSVSLV 490 500 510 520 530 540 LVGIVTLYLG VMVQADSGCV VSWKNKELKC GSGIFVTDNV HTWTEQYKFQ PESPSKLASA 550 560 570 580 590 600 IQKAHEEGIC GIRSVTRLEN LMWKQITSEL NHILSENEVK LTIMTGDIKG IMQVGKRSLR 610 620 630 640 650 660 PQPTELRYSW KTWGKAKMLS TELHNQTFLI DGPETAECPN TNRAWNSLEV EDYGFGVFTT 670 680 690 700 710 720 NIWLRLREKQ DVFCDSKLMS AAIKDNRAVH ADMGYWIESA LNDTWKIEKA SFIEVKSCHW 730 740 750 760 770 780 PKSHTLWSNG VLESEMVIPK NFAGPVSQHN NRPGYYTQTA GPWHLGKLEM DFDFCEGTTV 790 800 810 820 830 840 VVTEDCGNRG PSLRTTTASG KLITEWCCRS CTLPPLRYRG EDGCWYGMEI RPLKEKEENL 850 860 870 880 890 900 VSSLVTAGHG QIDNFSLGIL GMALFLEEML RTRVGTKHAI LLVAVSFVTL ITGNMSFRDL 910 920 930 940 950 960 GRVMVMVGAT MTDDIGMGVT YLALLAAFRV RPTFAAGLLL RKLTSKELMM TTIGIVLLSQ 970 980 990 1000 1010 1020 SSIPETILEL TDALALGMMV LKMVRNMEKY QLAVTIMAIL CVPNAVILQN AWKVSCTILA 1030 1040 1050 1060 1070 1080 VVSVSPLLLT SSQQKADWIP LALTIKGLNP TAIFLTTLSR TSKKRAGVLW DVPSPPPVGK 1090 1100 1110 1120 1130 1140 AELEDGAYRI KQKGILGYSQ IGAGVYKEGT FHTMWHVTRG AVLMHKGKRI EPSWADVKKD 1150 1160 1170 1180 1190 1200 LISYGGGWKL EGEWKEGEEV QVLALEPGKN PRAVQTKPGL FRTNTGTIGA VSLDFSPGTS 1210 1220 1230 1240 1250 1260 GSPIVDKKGK VVGLYGNGVV TRSGAYVSAI AQTEKSIEDN PEIEDDIFRK RRLTIMDLHP 1270 1280 1290 1300 1310 1320 GAGKTKRYLP AIVREAIKRG LRTLILAPTR VVAAEMEEAL RGLPIRYQTP AIRAEHTGRE 1330 1340 1350 1360 1370 1380 IVDLMCHATF TMRLLSPIRV PNYNLIIMDE AHFTDPASIA ARGYISTRVE MGEAAGIFMT 1390 1400 1410 1420 1430 1440 ATPPGSRDPF PQSNAPIMDE EREIPERSWN SGHEWVTDFK GKTVWFVPSI KTGNDIAACL 1450 1460 1470 1480 1490 1500 RKNGKRVIQL SRKTFDSEYV KTRTNDWDFV VTTDISEMGA NFKAERVIDP RRCMKPVILT 1510 1520 1530 1540 1550 1560 DGEERVILAG PMPVTHSSAA QRRGRIGRNP RNENDQYIYM GEPLENDEDC AHWKEAKMLL 1570 1580 1590 1600 1610 1620 DNINTPEGII PSIFEPEREK VDAIDGEYRL RGEARKTFVD LMRRGDLPVW LAYKVAAEGI 1630 1640 1650 1660 1670 1680 NYADRRWCFD GTRNNQILEE NVEVEIWTKE GERKKLKPRW LDARIYSDPL ALKEFKEFAA GRK

References

[1]	"Nucleotide sequence of the envelope glycoprotein gene of a dengue-2 virus isolated during an epidemic of benign dengue fever in Tonga in 1974." Chen W., Maguire T. Nucleic Acids Res. 18:5889-5889(1990) [PubMed: 2216784] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 1-495.
[2]	Qu X., Chen W., Maguire T. Submitted (MAR-1991) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 496-1683.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X54319 Genomic RNA. Translation: CAA38217.1.
X57469 Genomic RNA. Translation: CAA40705.1.
X57468 Genomic RNA. Translation: CAA40704.1.

PIR

PQ0507.
S11482.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1TG8	X-ray	2.61	A	1-395	[»]
1TGE	electron microscopy	12.50	A/B/C	1-395	[»]

SMR

P27914. Positions 1070-1246, 1236-1683.

ModBase

Search...

Family and domain databases

InterPro

IPR014001. DEAD-like_N.
IPR011492. DEAD_Flavivir.
IPR001650. DNA/RNA_helicase_C.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR013754. Flav_glyE_dim.
IPR001157. Flavi_NS1.
IPR000752. Flavi_NS2A.
IPR000336. Flv_glyE_Ig-like.
IPR011999. GlycoprotE_cen/dimer_Flavivir.
IPR014021. Helicase_SF1/SF2_ATP-bd.
IPR001850. Peptidase_S7.
[Graphical view]

Gene3D

G3DSA:2.60.98.10. Flav_glyE_dim. 1 hit.
G3DSA:2.60.40.350. Flv_glyE_Ig-like. 1 hit.

Pfam

PF07652. Flavi_DEAD. 1 hit.
PF02832. Flavi_glycop_C. 1 hit.
PF00869. Flavi_glycoprot. 1 hit.
PF00948. Flavi_NS1. 1 hit.
PF01005. Flavi_NS2A. 1 hit.
PF00949. Peptidase_S7. 1 hit.
[Graphical view]

ProDom

PD001496. Flavi_NS1. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]

PROSITE

PS00690. DEAH_ATP_HELICASE. False negative.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

POLG_DEN2T

Accession

Primary (citable) accession number: P27914

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	November 3, 2009
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)