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Protein

Neuraminidase

Gene

NA

Organism
Influenza B virus (strain B/Beijing/1/1987)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the removal of terminal sialic acid residues from viral and cellular glycoconjugates. Cleaves off the terminal sialic acids on the glycosylated HA during virus budding to facilitate virus release. Additionally helps virus spread through the circulation by further removing sialic acids from the cell surface. These cleavages prevent self-aggregation and ensure the efficient spread of the progeny virus from cell to cell. Otherwise, infection would be limited to one round of replication. Described as a receptor-destroying enzyme because it cleaves a terminal sialic acid from the cellular receptors. May facilitate viral invasion of the upper airways by cleaving the sialic acid moities on the mucin of the airway epithelial cells (By similarity).By similarity

Catalytic activityi

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

Cofactori

Ca2+1 PublicationNote: Binds 1 Ca2+ ion per subunit.1 Publication

Enzyme regulationi

Inhibited by the neuraminidase inhibitors zanamivir (Relenza) and oseltamivir (Tamiflu). These drugs interfere with the release of progeny virus from infected cells and are effective against all influenza strains. Resistance to neuraminidase inhibitors is quite rare.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei115Substrate1
Active sitei148Proton donor/acceptorBy similarity1
Binding sitei149Substrate1
Binding sitei291Substrate1
Metal bindingi292Calcium; via carbonyl oxygen1 Publication1
Metal bindingi296Calcium; via carbonyl oxygen1 Publication1
Metal bindingi323Calcium1 Publication1
Metal bindingi343Calcium; via carbonyl oxygen1 Publication1
Metal bindingi345Calcium; via carbonyl oxygen1 Publication1
Binding sitei373Substrate1
Active sitei408NucleophileBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.2.1.18. 7480.

Protein family/group databases

CAZyiGH34. Glycoside Hydrolase Family 34.

Names & Taxonomyi

Protein namesi
Recommended name:
Neuraminidase (EC:3.2.1.18)
Gene namesi
Name:NA
OrganismiInfluenza B virus (strain B/Beijing/1/1987)
Taxonomic identifieri11525 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus B
Virus hostiHomo sapiens (Human) [TaxID: 9606]

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 6IntravirionSequence analysis6
Transmembranei7 – 35Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST29
Topological domaini36 – 465Virion surfaceSequence analysisAdd BLAST430

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Virion

Pathology & Biotechi

Chemistry databases

DrugBankiDB06614. Peramivir.
DB00558. Zanamivir.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000787301 – 465NeuraminidaseAdd BLAST465

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi55N-linked (GlcNAc...); by hostSequence analysis1
Glycosylationi63N-linked (GlcNAc...); by hostSequence analysis1
Disulfide bondi86 ↔ 419
Disulfide bondi121 ↔ 126
Disulfide bondi181 ↔ 228
Disulfide bondi230 ↔ 235
Disulfide bondi276 ↔ 290
Disulfide bondi278 ↔ 288
Glycosylationi283N-linked (GlcNAc...); by host1 Publication1
Disulfide bondi317 ↔ 336
Disulfide bondi423 ↔ 446

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homotetramer.1 Publication

Structurei

Secondary structure

1465
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi89 – 97Combined sources9
Helixi99 – 102Combined sources4
Beta strandi111 – 121Combined sources11
Beta strandi126 – 140Combined sources15
Turni143 – 146Combined sources4
Beta strandi154 – 159Combined sources6
Turni166 – 168Combined sources3
Beta strandi170 – 174Combined sources5
Beta strandi176 – 182Combined sources7
Beta strandi187 – 194Combined sources8
Helixi196 – 198Combined sources3
Beta strandi200 – 205Combined sources6
Beta strandi208 – 214Combined sources7
Beta strandi216 – 219Combined sources4
Beta strandi229 – 231Combined sources3
Beta strandi234 – 242Combined sources9
Beta strandi244 – 246Combined sources3
Beta strandi248 – 256Combined sources9
Beta strandi259 – 264Combined sources6
Beta strandi267 – 269Combined sources3
Beta strandi274 – 291Combined sources18
Beta strandi293 – 295Combined sources3
Beta strandi300 – 305Combined sources6
Turni306 – 309Combined sources4
Beta strandi310 – 315Combined sources6
Beta strandi323 – 325Combined sources3
Beta strandi351 – 355Combined sources5
Beta strandi360 – 366Combined sources7
Beta strandi368 – 384Combined sources17
Turni386 – 388Combined sources3
Beta strandi394 – 405Combined sources12
Beta strandi409 – 415Combined sources7
Beta strandi417 – 430Combined sources14
Beta strandi439 – 447Combined sources9
Beta strandi449 – 451Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A4GX-ray2.20A/B76-465[»]
1A4QX-ray1.90A/B76-465[»]
1NSBX-ray2.20A/B76-465[»]
1NSCX-ray1.70A/B76-465[»]
1NSDX-ray1.80A/B76-465[»]
ProteinModelPortaliP27907.
SMRiP27907.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP27907.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni39 – 68Hypervariable stalk regionAdd BLAST30
Regioni69 – 465Head of neuraminidaseAdd BLAST397
Regioni274 – 275Substrate binding2

Sequence similaritiesi

Belongs to the glycosyl hydrolase 34 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Family and domain databases

CDDicd15483. Influenza_NA. 1 hit.
Gene3Di2.120.10.10. 1 hit.
InterProiIPR001860. Glyco_hydro_34.
IPR033654. Sialidase_Influenza_A/B.
IPR011040. Sialidases.
[Graphical view]
PfamiPF00064. Neur. 1 hit.
[Graphical view]
SUPFAMiSSF50939. SSF50939. 1 hit.

Sequencei

Sequence statusi: Complete.

P27907-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLPSTIQTLT LFLTSGGVLL SLYVSASLSY LLYSDILLKF SSKITAPTMT
60 70 80 90 100
LDCANASNVQ AVNRSATKEM TFLLPEPEWT YPRLSCQGST FQKALLISPH
110 120 130 140 150
RFGEARGNSA PLIIREPFIA CGPKECKHFA LTHYAAQPGG YYNGTREDRN
160 170 180 190 200
KLRHLISVKL GKIPTVENSI FHMAAWSGSA CHDGREWTYI GVDGPDSNAL
210 220 230 240 250
IKIKYGEAYT DTYHSYANNI LRTQESACNC IGGDCYLMIT DGSASGISKC
260 270 280 290 300
RFLKIREGRI IKEIFPTGRV EHTEECTCGF ASNKTIECAC RDNSYTAKRP
310 320 330 340 350
FVKLNVETDT AEIRLMCTET YLDTPRPDDG SITGPCESNG DKGRGGIKGG
360 370 380 390 400
FVHQRMASKI GRWYSRTMSK TERMGMELYV RYDGDPWTDS DALAHSGVMV
410 420 430 440 450
SMKEPGWYSF GFEIKDKKCD VPCIGIEMVH DGGKKTWHSA ATAIYCLMGS
460
GQLLWDTVTG VDMAL
Length:465
Mass (Da):51,430
Last modified:August 1, 1992 - v1
Checksum:i2FC60B895CBD430C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M54967 Genomic RNA. Translation: AAA43733.1.
PIRiB38520. NMIVB1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M54967 Genomic RNA. Translation: AAA43733.1.
PIRiB38520. NMIVB1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A4GX-ray2.20A/B76-465[»]
1A4QX-ray1.90A/B76-465[»]
1NSBX-ray2.20A/B76-465[»]
1NSCX-ray1.70A/B76-465[»]
1NSDX-ray1.80A/B76-465[»]
ProteinModelPortaliP27907.
SMRiP27907.
ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

DrugBankiDB06614. Peramivir.
DB00558. Zanamivir.

Protein family/group databases

CAZyiGH34. Glycoside Hydrolase Family 34.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi3.2.1.18. 7480.

Miscellaneous databases

EvolutionaryTraceiP27907.

Family and domain databases

CDDicd15483. Influenza_NA. 1 hit.
Gene3Di2.120.10.10. 1 hit.
InterProiIPR001860. Glyco_hydro_34.
IPR033654. Sialidase_Influenza_A/B.
IPR011040. Sialidases.
[Graphical view]
PfamiPF00064. Neur. 1 hit.
[Graphical view]
SUPFAMiSSF50939. SSF50939. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNRAM_INBBE
AccessioniPrimary (citable) accession number: P27907
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: November 2, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

The influenza B genome consist of 8 RNA segments. Genetic variation of hemagglutinin and/or neuraminidase genes results in the emergence of new influenza strains. The mechanism of variation can be the result of point mutations or the result of genetic reassortment between segments of two different strains.

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.