Trichome differentiation protein GL1 - Arabidopsis thaliana (Mouse-ear cress)

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P27900 (GL1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 119. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Trichome differentiation protein GL1

Alternative name(s):
Myb-related protein 0
Short name=AtMYB0
Protein GLABRA 1
Protein GLABROUS 1
R2R3-MYB transcription factor GL1

Gene names

Name:	GL1
Synonyms:	MYB0
Ordered Locus Names:	At3g27920
ORF Names:	K16N12.17

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	228 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcription activator, when associated with BHLH2/EGL3/MYC146 or BHLH12/MYC1. Involved in epidermal cell fate specification in leaves. Together with TTG1 and GL3, promotes trichome formation and endoreplication. Regulates the production of a signal that induces hair (trichome) precursor cells on leaf primordia to differentiate. Binds to the WER-binding sites (WBS) promoter regions and activates the transcription of target genes By similarity. Ref.9 Ref.11 Ref.14 Ref.15
Subunit structure	Interacts directly with GL3 and BHLH2. Part of a complex made of GL1, GL3 or BHLH2, and TTG1. Interacts also with BHLH2/EGL3/MYC146 and BHLH12/MYC1. Ref.9 Ref.12 Ref.13 Ref.14 Ref.15
Subcellular location	Nucleus. Note: Detected in trichome nucleus. Ref.13
Tissue specificity	Expressed in leaves, stems and flowers. Ref.10
Developmental stage	Ubiquitous in young leaves primordia. Becomes more prominent in developing trichome cells but disappears progressively when trichomes begin to initiate branches. Ref.15
Sequence similarities	Contains 2 HTH myb-type DNA-binding domains.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Domain	Repeat
Ligand	DNA-binding
Molecular function	Activator Developmental protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell fate specification Inferred from mutant phenotype PubMed 12244266. Source: TAIR gibberellic acid mediated signaling pathway Traceable author statement PubMed 9625690. Source: TAIR regulation of protein localization Inferred from mutant phenotype PubMed 18434419. Source: TAIR transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW trichome patterning Inferred from mutant phenotype PubMed 12244266. Source: TAIR
Cellular_component	nucleus Traceable author statement PubMed 9625690. Source: TAIR
Molecular_function	DNA binding Inferred from direct assay PubMed 18434419. Source: TAIR chromatin binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from sequence or structural similarity PubMed 11118137. Source: TAIR
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
GL3	Q9FN69	5	EBI-1543742,EBI-533348

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 228

228

Trichome differentiation protein GL1

PRO_0000197071

Regions

Domain

11 – 63

HTH myb-type 1

Domain

64 – 118

HTH myb-type 2

DNA binding

39 – 63

H-T-H motif By similarity

DNA binding

91 – 114

H-T-H motif By similarity

Natural variations

Natural variant

130 – 132

GED → VEE in strain: cv. Can-0.

Natural variant

148

Missing in strain: cv. Kas-1, cv. Ms-0. Ref.4

Natural variant

149

C → R in strain: cv. Aa-0, cv. An-1, cv. Bur-0, cv. Can-0, cv. Es-0, cv. Gr-1, cv. Hi-0, cv. Ita-0, cv. Kas-1, cv. Ms-0, cv. Mt-0, cv. Nd-0, cv. No-0, cv. Oy-0, cv. RLD, cv. Tsu-0, cv. Wassilewskija and cv. Yo-0. Ref.4

Natural variant

176

P → R in strain: cv. Di-0 and cv. Landsberg erecta. Ref.4

Natural variant

222 – 224

GDV → SDI in strain: cv. Aa-0, cv. An-1, cv. Bur-0, cv. Can-0, cv. Es-0, cv. Gr-1, cv. Hi-0, cv. Ita-0, cv. Kas-1, cv. Ms-0, cv. Mt-0, cv. Nd-0, cv. No-0, cv. Oy-0, cv. RLD, cv. Tsu-0, cv. Wassilewskija and cv. Yo-0.

Experimental info

Mutagenesis

100

G → R in gl1-65; few trichomes on leaf margins, cauline leaves, and stems. Ref.4

Mutagenesis

182 – 228

Missing in gl1-323; few trichomes on leaf margins, cauline leaves. Ref.2

Mutagenesis

202 – 228

Missing in gl1-2; reduced trichome density, truncated trichomes. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

P27900 [UniParc].

Last modified October 18, 2001. Version 2.
Checksum: 822A8646F446F6B9
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FASTA

228

26,339

        10         20         30         40         50         60 
MRIRRRDEKE NQEYKKGLWT VEEDNILMDY VLNHGTGQWN RIVRKTGLKR CGKSCRLRWM 

        70         80         90        100        110        120 
NYLSPNVNKG NFTEQEEDLI IRLHKLLGNR WSLIAKRVPG RTDNQVKNYW NTHLSKKLVG 

       130        140        150        160        170        180 
DYSSAVKTTG EDDDSPPSLF ITAATPSSCH HQQENIYENI AKSFNGVVSA SYEDKPKQEL 

       190        200        210        220 
AQKDVLMATT NDPSHYYGNN ALWVHDDDFE LSSLVMMNFA SGDVEYCL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A myb gene required for leaf trichome differentiation in Arabidopsis is expressed in stipules." Oppenheimer D.G., Herman P.L., Sivakumaran S., Esch J.J., Marks M.D. Cell 67:483-493(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Characterization of a weak allele of the GL1 gene of Arabidopsis thaliana." Esch J.J., Oppenheimer D.G., Marks M.D. Plant Mol. Biol. 24:203-207(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF 182-GLN--LEU-228 AND 202-LEU--LEU-228.
[3]	"Nucleotide sequence of the GLABROUS1 gene of Arabidopsis thaliana ecotype Columbia." Shikazono N., Tanaka A., Yokota Y., Watanabe H., Tano S. DNA Seq. 9:177-181(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Columbia.
[4]	"Trichome distribution in Arabidopsis thaliana and its close relative Arabidopsis lyrata: molecular analysis of the candidate gene GLABROUS1." Hauser M.-T., Harr B., Schloetterer C. Mol. Biol. Evol. 18:1754-1763(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF GLY-100, VARIANTS 130-VAL--GLU-132; SER-148 DEL; ARG-149; ARG-176 AND 222-SER--ILE-224. Strain: cv. Aa-0, cv. An-1, cv. Ba-1, cv. Bla-1, cv. Bur-0, cv. Can-0, cv. Columbia, cv. Di-0, cv. Es-0, cv. Gr-1, cv. Hi-0, cv. Ita-0, cv. Kas-1, cv. Kon, cv. Landsberg erecta, cv. Ms-0, cv. Mt-0, cv. Nd-0, cv. No-0, cv. Oy-0, cv. RLD, cv. Sha, cv. Te-0, cv. Tsu-0, cv. Wassilewskija and cv. Yo-0.
[5]	"The R2R3-MYB gene family in Arabidopsis thaliana." Stracke R., Werber M., Weisshaar B. Curr. Opin. Plant Biol. 4:447-456(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, NOMENCLATURE. Strain: cv. Columbia.
[6]	"The MYB transcription factor family in Arabidopsis: a genome-wide cloning and expression pattern analysis." Qu L.-J., Gu H. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[7]	"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones." Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S. DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[8]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[9]	"GL3 encodes a bHLH protein that regulates trichome development in arabidopsis through interaction with GL1 and TTG1." Payne C.T., Zhang F., Lloyd A.M. Genetics 156:1349-1362(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH GL3.
[10]	"Ectopic expression of the Arabidopsis AtMYB23 gene induces differentiation of trichome cells." Kirik V., Schnittger A., Radchuk V., Adler K., Huelskamp M., Baeumlein H. Dev. Biol. 235:366-377(2001) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY. Strain: cv. Landsberg erecta. Tissue: Silique.
[11]	"TRIPTYCHON and CAPRICE mediate lateral inhibition during trichome and root hair patterning in Arabidopsis." Schellmann S., Schnittger A., Kirik V., Wada T., Okada K., Beermann A., Thumfahrt J., Juergens G., Huelskamp M. EMBO J. 21:5036-5046(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION. Strain: cv. Columbia. Tissue: Root.
[12]	"A network of redundant bHLH proteins functions in all TTG1-dependent pathways of Arabidopsis." Zhang F., Gonzalez A., Zhao M., Payne C.T., Lloyd A.M. Development 130:4859-4869(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH BHLH2.
[13]	"A contradictory GLABRA3 allele helps define gene interactions controlling trichome development in Arabidopsis." Esch J.J., Chen M., Sanders M., Hillestad M., Ndkium S., Idelkope B., Neizer J., Marks M.D. Development 130:5885-5894(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH GL3, SUBCELLULAR LOCATION.
[14]	"Comprehensive identification of Arabidopsis thaliana MYB transcription factors interacting with R/B-like BHLH proteins." Zimmermann I.M., Heim M.A., Weisshaar B., Uhrig J.F. Plant J. 40:22-34(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH BHLH2 AND BHLH12.
[15]	"Functional diversification of MYB23 and GL1 genes in trichome morphogenesis and initiation." Kirik V., Lee M.M., Wester K., Herrmann U., Zheng Z., Oppenheimer D., Schiefelbein J., Hulskamp M. Development 132:1477-1485(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH GL3, DEVELOPMENTAL STAGE.
[16]	"The MYB transcription factor superfamily of Arabidopsis: expression analysis and phylogenetic comparison with the rice MYB family." Chen Y., Yang X., He K., Liu M., Li J., Gao Z., Lin Z., Zhang Y., Wang X., Qiu X., Shen Y., Zhang L., Deng X., Luo J., Deng X.-W., Chen Z., Gu H., Qu L.-J. Plant Mol. Biol. 60:107-124(2006) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY.
+	Additional computationally mapped references.

Web resources

Protein Spotlight

More to it than meets the finger - Issue 120 of August 2010

Cross-references

Sequence databases
EMBL GenBank DDBJ	M79448 Genomic DNA. Translation: AAC97387.1. L22786 Genomic DNA. Translation: AAC97388.1. AB006078 Genomic DNA. Translation: BAA86879.1. AJ243899 Genomic DNA. Translation: CAB97484.1. AJ243900 Genomic DNA. Translation: CAB97485.1. AF263690 Genomic DNA. Translation: AAL01215.1. AF263691 Genomic DNA. Translation: AAL01216.1. AF263692 Genomic DNA. Translation: AAL01217.1. AF263693 Genomic DNA. Translation: AAL01218.1. AF263694 Genomic DNA. Translation: AAL01219.1. AF263695 Genomic DNA. Translation: AAL01220.1. AF263696 Genomic DNA. Translation: AAL01221.1. AF263697 Genomic DNA. Translation: AAL01222.1. AF263698 Genomic DNA. Translation: AAL01223.1. AF263699 Genomic DNA. Translation: AAL01224.1. AF263700 Genomic DNA. Translation: AAL01225.1. AF263701 Genomic DNA. Translation: AAL01226.1. AF263702 Genomic DNA. Translation: AAL01227.1. AF263703 Genomic DNA. Translation: AAL01228.1. AF263704 Genomic DNA. Translation: AAL01229.1. AF263705 Genomic DNA. Translation: AAL01230.1. AF263706 Genomic DNA. Translation: AAL01231.1. AF263707 Genomic DNA. Translation: AAL01232.1. AF263708 Genomic DNA. Translation: AAL01233.1. AF263709 Genomic DNA. Translation: AAL01234.1. AF263710 Genomic DNA. Translation: AAL01235.1. AF263711 Genomic DNA. Translation: AAL01236.1. AF263712 Genomic DNA. Translation: AAL01237.1. AF263713 Genomic DNA. Translation: AAL01238.1. AF263714 Genomic DNA. Translation: AAL01239.1. AF263715 Genomic DNA. Translation: AAL01240.1. AF263718 Genomic DNA. Translation: AAL01243.1. AF263719 Genomic DNA. Translation: AAL01244.1. AF495524 mRNA. Translation: AAM14620.1. AY519590 mRNA. Translation: AAS10060.1. AP000371 Genomic DNA. Translation: BAB02538.1. CP002686 Genomic DNA. Translation: AEE77380.1.
IPI	IPI00548515.
PIR	TVMUG1. A39289.
RefSeq	NP_189430.1. NM_113708.1.
UniGene	At.42881.
3D structure databases
ProteinModelPortal	P27900.
SMR	P27900. Positions 16-118.
ModBase	Search...
Protein-protein interaction databases
IntAct	P27900. 5 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G27920.1; AT3G27920.1; AT3G27920.
GeneID	822415.
KEGG	ath:AT3G27920.
Organism-specific databases
GeneFarm	924. 100.
TAIR	At3g27920.
Phylogenomic databases
eggNOG	COG5147.
InParanoid	P27900.
KO	K09422.
OMA	YENIAKS.
PhylomeDB	P27900.
ProtClustDB	CLSN2685192.
Gene expression databases
ArrayExpress	P27900.
Genevestigator	P27900.
GermOnline	AT3G27920. Arabidopsis thaliana.
Family and domain databases
Gene3D	1.10.10.60. 2 hits.
InterPro	IPR009057. Homeodomain-like. IPR017930. Myb_dom. IPR001005. SANT/Myb. [Graphical view]
Pfam	PF00249. Myb_DNA-binding. 2 hits. [Graphical view]
SMART	SM00717. SANT. 2 hits. [Graphical view]
SUPFAM	SSF46689. Homeodomain_like. 2 hits.
PROSITE	PS51294. HTH_MYB. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GL1_ARATH

Accession

Primary (citable) accession number: P27900
Secondary accession number(s): Q39109

Q9ST42

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	October 18, 2001
Last modified:	May 1, 2013
This is version 119 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Natural variations

Experimental info

Sequences

References

Web resources

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents