Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Trichome differentiation protein GL1

Gene

GL1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription activator, when associated with BHLH2/EGL3/MYC146 or BHLH12/MYC1. Involved in epidermal cell fate specification in leaves. Together with TTG1 and GL3, promotes trichome formation and endoreplication. Regulates the production of a signal that induces hair (trichome) precursor cells on leaf primordia to differentiate. Binds to the WER-binding sites (WBS) promoter regions and activates the transcription of target genes (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi39 – 6325H-T-H motifPROSITE-ProRule annotationAdd
BLAST
DNA bindingi91 – 11424H-T-H motifPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. chromatin binding Source: InterPro
  2. DNA binding Source: TAIR
  3. sequence-specific DNA binding transcription factor activity Source: TAIR

GO - Biological processi

  1. cell fate specification Source: TAIR
  2. gibberellic acid mediated signaling pathway Source: TAIR
  3. regulation of protein localization Source: TAIR
  4. regulation of trichome morphogenesis Source: TAIR
  5. transcription, DNA-templated Source: UniProtKB-KW
  6. trichome patterning Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Trichome differentiation protein GL1
Alternative name(s):
Myb-related protein 0
Short name:
AtMYB0
Protein GLABRA 1
Protein GLABROUS 1
R2R3-MYB transcription factor GL1
Gene namesi
Name:GL1
Synonyms:MYB0
Ordered Locus Names:At3g27920
ORF Names:K16N12.17
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G27920.

Subcellular locationi

Nucleus PROSITE-ProRule annotation1 Publication
Note: Detected in trichome nucleus.

GO - Cellular componenti

  1. nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi100 – 1001G → R in gl1-65; few trichomes on leaf margins, cauline leaves, and stems. 1 Publication
Mutagenesisi182 – 22847Missing in gl1-323; few trichomes on leaf margins, cauline leaves. 1 PublicationAdd
BLAST
Mutagenesisi202 – 22827Missing in gl1-2; reduced trichome density, truncated trichomes. 1 PublicationAdd
BLAST

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 228228Trichome differentiation protein GL1PRO_0000197071Add
BLAST

Proteomic databases

PRIDEiP27900.

Expressioni

Tissue specificityi

Expressed in leaves, stems and flowers.1 Publication

Developmental stagei

Ubiquitous in young leaves primordia. Becomes more prominent in developing trichome cells but disappears progressively when trichomes begin to initiate branches.1 Publication

Gene expression databases

ExpressionAtlasiP27900. baseline and differential.
GenevestigatoriP27900.

Interactioni

Subunit structurei

Homodimer and heterodimer with MYB82 (PubMed:24803498). Interacts directly with GL3 and BHLH2. Part of a complex made of GL1, GL3 or BHLH2, and TTG1. Interacts also with BHLH2/EGL3/MYC146 and BHLH12/MYC1. Interacts with MYB82 (PubMed:24803498).6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
GL3Q9FN695EBI-1543742,EBI-533348

Protein-protein interaction databases

BioGridi7745. 6 interactions.
IntActiP27900. 5 interactions.

Structurei

3D structure databases

ProteinModelPortaliP27900.
SMRiP27900. Positions 16-118.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 6353HTH myb-type 1PROSITE-ProRule annotationAdd
BLAST
Domaini64 – 11855HTH myb-type 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 HTH myb-type DNA-binding domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG5147.
InParanoidiP27900.
KOiK09422.
OMAiYENIAKS.
PhylomeDBiP27900.

Family and domain databases

Gene3Di1.10.10.60. 2 hits.
InterProiIPR009057. Homeodomain-like.
IPR017930. Myb_dom.
IPR001005. SANT/Myb.
[Graphical view]
PfamiPF00249. Myb_DNA-binding. 2 hits.
[Graphical view]
SMARTiSM00717. SANT. 2 hits.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS51294. HTH_MYB. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P27900-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRIRRRDEKE NQEYKKGLWT VEEDNILMDY VLNHGTGQWN RIVRKTGLKR
60 70 80 90 100
CGKSCRLRWM NYLSPNVNKG NFTEQEEDLI IRLHKLLGNR WSLIAKRVPG
110 120 130 140 150
RTDNQVKNYW NTHLSKKLVG DYSSAVKTTG EDDDSPPSLF ITAATPSSCH
160 170 180 190 200
HQQENIYENI AKSFNGVVSA SYEDKPKQEL AQKDVLMATT NDPSHYYGNN
210 220
ALWVHDDDFE LSSLVMMNFA SGDVEYCL
Length:228
Mass (Da):26,339
Last modified:October 18, 2001 - v2
Checksum:i822A8646F446F6B9
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti130 – 1323GED → VEE in strain: cv. Can-0.
Natural varianti148 – 1481Missing in strain: cv. Kas-1, cv. Ms-0. 1 Publication
Natural varianti149 – 1491C → R in strain: cv. Aa-0, cv. An-1, cv. Bur-0, cv. Can-0, cv. Es-0, cv. Gr-1, cv. Hi-0, cv. Ita-0, cv. Kas-1, cv. Ms-0, cv. Mt-0, cv. Nd-0, cv. No-0, cv. Oy-0, cv. RLD, cv. Tsu-0, cv. Wassilewskija and cv. Yo-0. 1 Publication
Natural varianti176 – 1761P → R in strain: cv. Di-0 and cv. Landsberg erecta. 1 Publication
Natural varianti222 – 2243GDV → SDI in strain: cv. Aa-0, cv. An-1, cv. Bur-0, cv. Can-0, cv. Es-0, cv. Gr-1, cv. Hi-0, cv. Ita-0, cv. Kas-1, cv. Ms-0, cv. Mt-0, cv. Nd-0, cv. No-0, cv. Oy-0, cv. RLD, cv. Tsu-0, cv. Wassilewskija and cv. Yo-0.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M79448 Genomic DNA. Translation: AAC97387.1.
L22786 Genomic DNA. Translation: AAC97388.1.
AB006078 Genomic DNA. Translation: BAA86879.1.
AJ243899 Genomic DNA. Translation: CAB97484.1.
AJ243900 Genomic DNA. Translation: CAB97485.1.
AF263690 Genomic DNA. Translation: AAL01215.1.
AF263691 Genomic DNA. Translation: AAL01216.1.
AF263692 Genomic DNA. Translation: AAL01217.1.
AF263693 Genomic DNA. Translation: AAL01218.1.
AF263694 Genomic DNA. Translation: AAL01219.1.
AF263695 Genomic DNA. Translation: AAL01220.1.
AF263696 Genomic DNA. Translation: AAL01221.1.
AF263697 Genomic DNA. Translation: AAL01222.1.
AF263698 Genomic DNA. Translation: AAL01223.1.
AF263699 Genomic DNA. Translation: AAL01224.1.
AF263700 Genomic DNA. Translation: AAL01225.1.
AF263701 Genomic DNA. Translation: AAL01226.1.
AF263702 Genomic DNA. Translation: AAL01227.1.
AF263703 Genomic DNA. Translation: AAL01228.1.
AF263704 Genomic DNA. Translation: AAL01229.1.
AF263705 Genomic DNA. Translation: AAL01230.1.
AF263706 Genomic DNA. Translation: AAL01231.1.
AF263707 Genomic DNA. Translation: AAL01232.1.
AF263708 Genomic DNA. Translation: AAL01233.1.
AF263709 Genomic DNA. Translation: AAL01234.1.
AF263710 Genomic DNA. Translation: AAL01235.1.
AF263711 Genomic DNA. Translation: AAL01236.1.
AF263712 Genomic DNA. Translation: AAL01237.1.
AF263713 Genomic DNA. Translation: AAL01238.1.
AF263714 Genomic DNA. Translation: AAL01239.1.
AF263715 Genomic DNA. Translation: AAL01240.1.
AF263718 Genomic DNA. Translation: AAL01243.1.
AF263719 Genomic DNA. Translation: AAL01244.1.
AF495524 mRNA. Translation: AAM14620.1.
AY519590 mRNA. Translation: AAS10060.1.
AP000371 Genomic DNA. Translation: BAB02538.1.
CP002686 Genomic DNA. Translation: AEE77380.1.
PIRiA39289. TVMUG1.
RefSeqiNP_189430.1. NM_113708.1.
UniGeneiAt.42881.

Genome annotation databases

EnsemblPlantsiAT3G27920.1; AT3G27920.1; AT3G27920.
GeneIDi822415.
KEGGiath:AT3G27920.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

Protein Spotlight

More to it than meets the finger - Issue 120 of August 2010

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M79448 Genomic DNA. Translation: AAC97387.1.
L22786 Genomic DNA. Translation: AAC97388.1.
AB006078 Genomic DNA. Translation: BAA86879.1.
AJ243899 Genomic DNA. Translation: CAB97484.1.
AJ243900 Genomic DNA. Translation: CAB97485.1.
AF263690 Genomic DNA. Translation: AAL01215.1.
AF263691 Genomic DNA. Translation: AAL01216.1.
AF263692 Genomic DNA. Translation: AAL01217.1.
AF263693 Genomic DNA. Translation: AAL01218.1.
AF263694 Genomic DNA. Translation: AAL01219.1.
AF263695 Genomic DNA. Translation: AAL01220.1.
AF263696 Genomic DNA. Translation: AAL01221.1.
AF263697 Genomic DNA. Translation: AAL01222.1.
AF263698 Genomic DNA. Translation: AAL01223.1.
AF263699 Genomic DNA. Translation: AAL01224.1.
AF263700 Genomic DNA. Translation: AAL01225.1.
AF263701 Genomic DNA. Translation: AAL01226.1.
AF263702 Genomic DNA. Translation: AAL01227.1.
AF263703 Genomic DNA. Translation: AAL01228.1.
AF263704 Genomic DNA. Translation: AAL01229.1.
AF263705 Genomic DNA. Translation: AAL01230.1.
AF263706 Genomic DNA. Translation: AAL01231.1.
AF263707 Genomic DNA. Translation: AAL01232.1.
AF263708 Genomic DNA. Translation: AAL01233.1.
AF263709 Genomic DNA. Translation: AAL01234.1.
AF263710 Genomic DNA. Translation: AAL01235.1.
AF263711 Genomic DNA. Translation: AAL01236.1.
AF263712 Genomic DNA. Translation: AAL01237.1.
AF263713 Genomic DNA. Translation: AAL01238.1.
AF263714 Genomic DNA. Translation: AAL01239.1.
AF263715 Genomic DNA. Translation: AAL01240.1.
AF263718 Genomic DNA. Translation: AAL01243.1.
AF263719 Genomic DNA. Translation: AAL01244.1.
AF495524 mRNA. Translation: AAM14620.1.
AY519590 mRNA. Translation: AAS10060.1.
AP000371 Genomic DNA. Translation: BAB02538.1.
CP002686 Genomic DNA. Translation: AEE77380.1.
PIRiA39289. TVMUG1.
RefSeqiNP_189430.1. NM_113708.1.
UniGeneiAt.42881.

3D structure databases

ProteinModelPortaliP27900.
SMRiP27900. Positions 16-118.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi7745. 6 interactions.
IntActiP27900. 5 interactions.

Proteomic databases

PRIDEiP27900.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G27920.1; AT3G27920.1; AT3G27920.
GeneIDi822415.
KEGGiath:AT3G27920.

Organism-specific databases

GeneFarmi924. 100.
TAIRiAT3G27920.

Phylogenomic databases

eggNOGiCOG5147.
InParanoidiP27900.
KOiK09422.
OMAiYENIAKS.
PhylomeDBiP27900.

Gene expression databases

ExpressionAtlasiP27900. baseline and differential.
GenevestigatoriP27900.

Family and domain databases

Gene3Di1.10.10.60. 2 hits.
InterProiIPR009057. Homeodomain-like.
IPR017930. Myb_dom.
IPR001005. SANT/Myb.
[Graphical view]
PfamiPF00249. Myb_DNA-binding. 2 hits.
[Graphical view]
SMARTiSM00717. SANT. 2 hits.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS51294. HTH_MYB. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A myb gene required for leaf trichome differentiation in Arabidopsis is expressed in stipules."
    Oppenheimer D.G., Herman P.L., Sivakumaran S., Esch J.J., Marks M.D.
    Cell 67:483-493(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Characterization of a weak allele of the GL1 gene of Arabidopsis thaliana."
    Esch J.J., Oppenheimer D.G., Marks M.D.
    Plant Mol. Biol. 24:203-207(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF 182-GLN--LEU-228 AND 202-LEU--LEU-228.
  3. "Nucleotide sequence of the GLABROUS1 gene of Arabidopsis thaliana ecotype Columbia."
    Shikazono N., Tanaka A., Yokota Y., Watanabe H., Tano S.
    DNA Seq. 9:177-181(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Columbia.
  4. "Trichome distribution in Arabidopsis thaliana and its close relative Arabidopsis lyrata: molecular analysis of the candidate gene GLABROUS1."
    Hauser M.-T., Harr B., Schloetterer C.
    Mol. Biol. Evol. 18:1754-1763(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF GLY-100, VARIANTS 130-VAL--GLU-132; SER-148 DEL; ARG-149; ARG-176 AND 222-SER--ILE-224.
    Strain: cv. Aa-0, cv. An-1, cv. Ba-1, cv. Bla-1, cv. Bur-0, cv. Can-0, cv. Columbia, cv. Di-0, cv. Es-0, cv. Gr-1, cv. Hi-0, cv. Ita-0, cv. Kas-1, cv. Kon, cv. Landsberg erecta, cv. Ms-0, cv. Mt-0, cv. Nd-0, cv. No-0, cv. Oy-0, cv. RLD, cv. Sha, cv. Te-0, cv. Tsu-0, cv. Wassilewskija and cv. Yo-0.
  5. "The R2R3-MYB gene family in Arabidopsis thaliana."
    Stracke R., Werber M., Weisshaar B.
    Curr. Opin. Plant Biol. 4:447-456(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, NOMENCLATURE.
    Strain: cv. Columbia.
  6. "The MYB transcription factor family in Arabidopsis: a genome-wide cloning and expression pattern analysis."
    Qu L.-J., Gu H.
    Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  7. "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
    Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
    DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  8. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  9. "GL3 encodes a bHLH protein that regulates trichome development in arabidopsis through interaction with GL1 and TTG1."
    Payne C.T., Zhang F., Lloyd A.M.
    Genetics 156:1349-1362(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH GL3.
  10. "Ectopic expression of the Arabidopsis AtMYB23 gene induces differentiation of trichome cells."
    Kirik V., Schnittger A., Radchuk V., Adler K., Huelskamp M., Baeumlein H.
    Dev. Biol. 235:366-377(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
    Strain: cv. Landsberg erecta.
    Tissue: Silique.
  11. "TRIPTYCHON and CAPRICE mediate lateral inhibition during trichome and root hair patterning in Arabidopsis."
    Schellmann S., Schnittger A., Kirik V., Wada T., Okada K., Beermann A., Thumfahrt J., Juergens G., Huelskamp M.
    EMBO J. 21:5036-5046(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: cv. Columbia.
    Tissue: Root.
  12. "A network of redundant bHLH proteins functions in all TTG1-dependent pathways of Arabidopsis."
    Zhang F., Gonzalez A., Zhao M., Payne C.T., Lloyd A.M.
    Development 130:4859-4869(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH BHLH2.
  13. "A contradictory GLABRA3 allele helps define gene interactions controlling trichome development in Arabidopsis."
    Esch J.J., Chen M., Sanders M., Hillestad M., Ndkium S., Idelkope B., Neizer J., Marks M.D.
    Development 130:5885-5894(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GL3, SUBCELLULAR LOCATION.
  14. "Comprehensive identification of Arabidopsis thaliana MYB transcription factors interacting with R/B-like BHLH proteins."
    Zimmermann I.M., Heim M.A., Weisshaar B., Uhrig J.F.
    Plant J. 40:22-34(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH BHLH2 AND BHLH12.
  15. "Functional diversification of MYB23 and GL1 genes in trichome morphogenesis and initiation."
    Kirik V., Lee M.M., Wester K., Herrmann U., Zheng Z., Oppenheimer D., Schiefelbein J., Hulskamp M.
    Development 132:1477-1485(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH GL3, DEVELOPMENTAL STAGE.
  16. "The MYB transcription factor superfamily of Arabidopsis: expression analysis and phylogenetic comparison with the rice MYB family."
    Chen Y., Yang X., He K., Liu M., Li J., Gao Z., Lin Z., Zhang Y., Wang X., Qiu X., Shen Y., Zhang L., Deng X., Luo J., Deng X.-W., Chen Z., Gu H., Qu L.-J.
    Plant Mol. Biol. 60:107-124(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY.
  17. "MYB82 functions in regulation of trichome development in Arabidopsis."
    Liang G., He H., Li Y., Ai Q., Yu D.
    J. Exp. Bot. 65:3215-3223(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MYB82, SUBUNIT.
    Strain: cv. Columbia.

Entry informationi

Entry nameiGL1_ARATH
AccessioniPrimary (citable) accession number: P27900
Secondary accession number(s): Q39109
, Q53YQ0, Q93V34, Q93VW0, Q93WP0, Q947R6, Q9LF81, Q9LF82, Q9ST42
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: October 18, 2001
Last modified: March 4, 2015
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.