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Protein

Nitrate/nitrite sensor protein NarQ

Gene

narQ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Acts as a sensor for nitrate/nitrite and transduces signal of nitrate/nitrite availability to the NarL/NarP proteins. NarQ probably activates NarL and NarP by phosphorylation. NarQ probably negatively regulates the NarL protein by dephosphorylation.

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • phosphorelay sensor kinase activity Source: EcoCyc

GO - Biological processi

  • cellular response to nitrate Source: EcoCyc
  • cellular response to nitrite Source: EcoCyc
  • nitrate assimilation Source: UniProtKB-KW
  • phosphorelay signal transduction system Source: EcoCyc
  • signal transduction by protein phosphorylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Nitrate assimilation, Two-component regulatory system

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:NARQ-MONOMER.
ECOL316407:JW2453-MONOMER.
BRENDAi2.7.13.3. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrate/nitrite sensor protein NarQ (EC:2.7.13.3)
Gene namesi
Name:narQ
Ordered Locus Names:b2469, JW2453
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11460. narQ.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1313CytoplasmicSequence analysisAdd
BLAST
Transmembranei14 – 3421HelicalSequence analysisAdd
BLAST
Topological domaini35 – 146112PeriplasmicSequence analysisAdd
BLAST
Transmembranei147 – 16721HelicalSequence analysisAdd
BLAST
Topological domaini168 – 566399CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • intracellular Source: GOC
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 566566Nitrate/nitrite sensor protein NarQPRO_0000074810Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei370 – 3701Phosphohistidine; by autocatalysisPROSITE-ProRule annotation

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP27896.

Interactioni

Protein-protein interaction databases

BioGridi4260928. 8 interactions.
DIPiDIP-10318N.
IntActiP27896. 12 interactions.
MINTiMINT-1307707.
STRINGi511145.b2469.

Structurei

3D structure databases

ProteinModelPortaliP27896.
SMRiP27896. Positions 349-554.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini174 – 22754HAMPPROSITE-ProRule annotationAdd
BLAST
Domaini364 – 559196Histidine kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 HAMP domain.PROSITE-ProRule annotation
Contains 1 histidine kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
ENOG410XNMH. LUCA.
HOGENOMiHOG000275465.
InParanoidiP27896.
KOiK07674.
OMAiFLQIQLT.
OrthoDBiEOG6JX7HQ.
PhylomeDBiP27896.

Family and domain databases

Gene3Di3.30.565.10. 1 hit.
InterProiIPR003660. HAMP_dom.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR029095. NarX-like_N.
IPR011712. Sig_transdc_His_kin_sub3_dim/P.
[Graphical view]
PfamiPF00672. HAMP. 1 hit.
PF02518. HATPase_c. 1 hit.
PF07730. HisKA_3. 1 hit.
PF13675. PilJ. 1 hit.
[Graphical view]
SMARTiSM00304. HAMP. 1 hit.
SM00387. HATPase_c. 1 hit.
[Graphical view]
SUPFAMiSSF55874. SSF55874. 1 hit.
PROSITEiPS50885. HAMP. 1 hit.
PS50109. HIS_KIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P27896-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIVKRPVSAS LARAFFYIVL LSILSTGIAL LTLASSLRDA EAINIAGSLR
60 70 80 90 100
MQSYRLGYDL QSGSPQLNAH RQLFQQALHS PVLTNLNVWY VPEAVKTRYA
110 120 130 140 150
HLNANWLEMN NRLSKGDLPW YQANINNYVN QIDLFVLALQ HYAERKMLLV
160 170 180 190 200
VAISLAGGIG IFTLVFFTLR RIRHQVVAPL NQLVTASQRI EHGQFDSPPL
210 220 230 240 250
DTNLPNELGL LAKTFNQMSS ELHKLYRSLE ASVEEKTRDL HEAKRRLEVL
260 270 280 290 300
YQCSQALNTS QIDVHCFRHI LQIVRDNEAA EYLELNVGEN WRISEGQPNP
310 320 330 340 350
ELPMQILPVT MQETVYGELH WQNSHVSSSE PLLNSVSSML GRGLYFNQAQ
360 370 380 390 400
KHFQQLLLME ERATIARELH DSLAQVLSYL RIQLTLLKRS IPEDNATAQS
410 420 430 440 450
IMADFSQALN DAYRQLRELL TTFRLTLQQA DLPSALREML DTLQNQTSAK
460 470 480 490 500
LTLDCRLPTL ALDAQMQVHL LQIIREAVLN AMKHANASEI AVSCVTAPDG
510 520 530 540 550
NHTVYIRDNG IGIGEPKEPE GHYGLNIMRE RAERLGGTLT FSQPSGGGTL
560
VSISFRSAEG EESQLM
Length:566
Mass (Da):63,697
Last modified:August 1, 1992 - v1
Checksum:i1D08EA5BD328ACE2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M94724 Genomic DNA. Translation: AAA24202.1.
X65714 Genomic DNA. Translation: CAA46630.1.
U00096 Genomic DNA. Translation: AAC75522.1.
AP009048 Genomic DNA. Translation: BAA16343.1.
U12598 Genomic DNA. Translation: AAA20583.1.
PIRiD65022.
RefSeqiNP_416964.1. NC_000913.3.
WP_001300881.1. NZ_CP014272.1.

Genome annotation databases

EnsemblBacteriaiAAC75522; AAC75522; b2469.
BAA16343; BAA16343; BAA16343.
GeneIDi946948.
KEGGiecj:JW2453.
eco:b2469.
PATRICi32120321. VBIEscCol129921_2563.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M94724 Genomic DNA. Translation: AAA24202.1.
X65714 Genomic DNA. Translation: CAA46630.1.
U00096 Genomic DNA. Translation: AAC75522.1.
AP009048 Genomic DNA. Translation: BAA16343.1.
U12598 Genomic DNA. Translation: AAA20583.1.
PIRiD65022.
RefSeqiNP_416964.1. NC_000913.3.
WP_001300881.1. NZ_CP014272.1.

3D structure databases

ProteinModelPortaliP27896.
SMRiP27896. Positions 349-554.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260928. 8 interactions.
DIPiDIP-10318N.
IntActiP27896. 12 interactions.
MINTiMINT-1307707.
STRINGi511145.b2469.

Proteomic databases

PaxDbiP27896.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75522; AAC75522; b2469.
BAA16343; BAA16343; BAA16343.
GeneIDi946948.
KEGGiecj:JW2453.
eco:b2469.
PATRICi32120321. VBIEscCol129921_2563.

Organism-specific databases

EchoBASEiEB1429.
EcoGeneiEG11460. narQ.

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
ENOG410XNMH. LUCA.
HOGENOMiHOG000275465.
InParanoidiP27896.
KOiK07674.
OMAiFLQIQLT.
OrthoDBiEOG6JX7HQ.
PhylomeDBiP27896.

Enzyme and pathway databases

BioCyciEcoCyc:NARQ-MONOMER.
ECOL316407:JW2453-MONOMER.
BRENDAi2.7.13.3. 2026.

Miscellaneous databases

PROiP27896.

Family and domain databases

Gene3Di3.30.565.10. 1 hit.
InterProiIPR003660. HAMP_dom.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR029095. NarX-like_N.
IPR011712. Sig_transdc_His_kin_sub3_dim/P.
[Graphical view]
PfamiPF00672. HAMP. 1 hit.
PF02518. HATPase_c. 1 hit.
PF07730. HisKA_3. 1 hit.
PF13675. PilJ. 1 hit.
[Graphical view]
SMARTiSM00304. HAMP. 1 hit.
SM00387. HATPase_c. 1 hit.
[Graphical view]
SUPFAMiSSF55874. SSF55874. 1 hit.
PROSITEiPS50885. HAMP. 1 hit.
PS50109. HIS_KIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Either of two functionally redundant sensor proteins, NarX and NarQ, is sufficient for nitrate regulation in Escherichia coli K-12."
    Rabin R.S., Stewart V.
    Proc. Natl. Acad. Sci. U.S.A. 89:8419-8423(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Identification and characterization of narQ, a second nitrate sensor for nitrate-dependent gene regulation in Escherichia coli."
    Chiang R.C., Cavicchioli R., Gunsalus R.P.
    Mol. Microbiol. 6:1913-1923(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
  3. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
    Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
    , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
    DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. Nilles M.L., Bertrand K.P.
    Submitted (JUL-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 547-566.
    Strain: K12.
  7. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiNARQ_ECOLI
AccessioniPrimary (citable) accession number: P27896
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: May 11, 2016
This is version 143 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.