P27895 (CIN8_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Kinesin-like protein CIN8

Alternative name(s):
Chromosome instability protein 8

Gene names

Name:	CIN8
Synonyms:	KSL2
Ordered Locus Names:	YEL061C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	1000 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for assembly of the mitotic spindle. Interacts with spindle microtubules to produce an outwardly directed force acting upon the poles. Following spindle assembly, CIN8 and KIP1 apparently act to oppose a force that draws separated poles back together. This force seems to be mediated by KAR3. Ref.1 Ref.5
Subcellular location	Cytoplasm › cytoskeleton › spindle. Mitochondrion. Note: Spindle microtubules that lie between the poles. Ref.1 Ref.7 Ref.9
Miscellaneous	Present with 238 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the kinesin-like protein family. BimC subfamily. Contains 1 kinesin-motor domain.
Sequence caution	The sequence AAA34496.1 differs from that shown. Reason: Erroneous initiation. The sequence AAB65026.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA77885.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Cytoplasm Cytoskeleton Microtubule Mitochondrion
Domain	Coiled coil
Ligand	ATP-binding Nucleotide-binding
Molecular function	Motor protein
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell division Inferred from electronic annotation. Source: UniProtKB-KW microtubule depolymerization Inferred from mutant phenotype PubMed 19041752. Source: SGD microtubule-based movement Inferred from electronic annotation. Source: InterPro mitotic anaphase B Inferred from mutant phenotype PubMed 9813090. Source: SGD mitotic sister chromatid segregation Inferred from genetic interaction PubMed 7860634. Source: SGD mitotic spindle organization in nucleus Traceable author statement PubMed 9153752. Source: SGD spindle pole body separation Inferred from mutant phenotype Ref.1. Source: SGD
Cellular_component	astral microtubule Inferred from mutant phenotype PubMed 19041752. Source: SGD condensed nuclear chromosome kinetochore Inferred from direct assay PubMed 11511347. Source: SGD kinesin complex Traceable author statement PubMed 9153752. Source: SGD kinetochore microtubule Inferred from direct assay PubMed 19041752. Source: SGD mitochondrion Inferred from direct assay Ref.9PubMed 16823961. Source: SGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW minus-end-directed microtubule motor activity Inferred from direct assay PubMed 21350123. Source: SGD plus-end-directed microtubule motor activity Inferred from direct assay PubMed 21350123. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1000	1000	Kinesin-like protein CIN8		PRO_0000125367
Regions
	Domain	33 – 408	376	Kinesin-motor
	Nucleotide binding	128 – 135	8	ATP By similarity
	Coiled coil	518 – 615	98	Potential
	Coiled coil	860 – 904	45	Potential
Amino acid modifications
	Modified residue	972	1	Phosphoserine Ref.10
Experimental info
	Sequence conflict	216	1	D → A in AAA34496. Ref.1
	Sequence conflict	216	1	D → A in CAA77885. Ref.1
	Sequence conflict	793	1	Q → H in AAA34496. Ref.1
	Sequence conflict	793	1	Q → H in CAA77885. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P27895 [UniParc]. Last modified January 10, 2006. Version 3. Checksum: B33141C45748CE67	FASTA	1,000	113,311
	10 20 30 40 50 60 MPAENQNTGQ DRSSNSISKN GNSQVGCHTV PNEELNITVA VRCRGRNERE ISMKSSVVVN 70 80 90 100 110 120 VPDITGSKEI SINTTGDTGI TAQMNAKRYT VDKVFGPGAS QDLIFDEVAG PLFQDFIKGY 130 140 150 160 170 180 NCTVLVYGMT STGKTYTMTG DEKLYNGELS DAAGIIPRVL LKLFDTLELQ QNDYVVKCSF 190 200 210 220 230 240 IELYNEELKD LLDSNSNGSS NTGFDGQFMK KLRIFDSSTA NNTTSNSASS SRSNSRNSSP 250 260 270 280 290 300 RSLNDLTPKA ALLRKRLRTK SLPNTIKQQY QQQQAVNSRN NSSSNSGSTT NNASSNTNTN 310 320 330 340 350 360 NGQRSSMAPN DQTNGIYIQN LQEFHITNAM EGLNLLQKGL KHRQVASTKM NDFSSRSHTI 370 380 390 400 410 420 FTITLYKKHQ DELFRISKMN LVDLAGSENI NRSGALNQRA KEAGSINQSL LTLGRVINAL 430 440 450 460 470 480 VDKSGHIPFR ESKLTRLLQD SLGGNTKTAL IATISPAKVT SEETCSTLEY ASKAKNIKNK 490 500 510 520 530 540 PQLGSFIMKD ILVKNITMEL AKIKSDLLST KSKEGIYMSQ DHYKNLNSDL ESYKNEVQEC 550 560 570 580 590 600 KREIESLTSK NALLVKDKLK SKETIQSQNC QIESLKTTID HLRAQLDKQH KTEIEISDFN 610 620 630 640 650 660 NKLQKLTEVM QMALHDYKKR ELDLNQKFEM HITKEIKKLK STLFLQLNTM QQESILQETN 670 680 690 700 710 720 IQPNLDMIKN EVLTLMRTMQ EKAELMYKDC VKKILNESPK FFNVVIEKID IIRVDFQKFY 730 740 750 760 770 780 KNIAENLSDI SEENNNMKQY LKNHFFKNNH QELLNRHVDS TYENIEKRTN EFVENFKKVL 790 800 810 820 830 840 NDHLDENKKL IMQNLTTATS AVIDQEMDLF EPKRVKWENS FDLINDCDSM NNEFYNSMAA 850 860 870 880 890 900 TLSQIKSTVD TSSNSMNESI SVMKGQVEES ENAISLLKNN TKFNDQFEQL INKHNMLKDN 910 920 930 940 950 960 IKNSITSTHS HITNVDDIYN TIENIMKNYG NKENATKDEM IENILKEIPN LSKKMPLRLS 970 980 990 1000 NINSNSVQSV ISPKKHAIED ENKSSENVDN EGSRKMLKIE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two Saccharomyces cerevisiae kinesin-related gene products required for mitotic spindle assembly." Hoyt M.A., He L., Loo K.K., Saunders W.S. J. Cell Biol. 118:109-120(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"A second nitrogen permease regulator in Saccharomyces cerevisiae." Rousselet G., Simon M., Ripoche P., Buhler J.-M. FEBS Lett. 359:215-219(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 983-1000. Strain: ATCC 204508 / S288c.
[5]	"Kinesin-related proteins required for structural integrity of the mitotic spindle." Saunders W.S., Hoyt M.A. Cell 70:451-458(1992) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION. Strain: ATCC 204508 / S288c.
[6]	"Sequencing and comparison of yeast species to identify genes and regulatory elements." Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S. Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"The proteome of Saccharomyces cerevisiae mitochondria." Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C. Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Strain: ATCC 76625 / YPH499.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-972, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M90522 Genomic DNA. Translation: AAA34496.1. Different initiation. Z11859 Genomic DNA. Translation: CAA77885.1. Different initiation. U18795 Genomic DNA. Translation: AAB65026.1. Different initiation. X79105 Genomic DNA. Translation: CAA55722.1. BK006939 Genomic DNA. Translation: DAA07593.1.
PIR	B42641. S50528.
RefSeq	NP_010853.2. NM_001178876.1.
3D structure databases
ProteinModelPortal	P27895.
SMR	P27895. Positions 36-223, 315-483.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3004N.
IntAct	P27895. 7 interactions.
MINT	MINT-435854.
STRING	4932.YEL061C.
Proteomic databases
PaxDb	P27895.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YEL061C; YEL061C; YEL061C.
GeneID	856648.
KEGG	sce:YEL061C.
Organism-specific databases
CYGD	YEL061c.
SGD	S000000787. CIN8.
Phylogenomic databases
eggNOG	COG5059.
GeneTree	ENSGT00680000099754.
HOGENOM	HOG000248614.
OMA	QVASTKM.
OrthoDB	EOG42C1HN.
Enzyme and pathway databases
BioCyc	YEAST:G3O-30176-MONOMER.
Gene expression databases
Genevestigator	P27895.
GermOnline	YEL061C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.40.850.10. 2 hits.
InterPro	IPR019821. Kinesin_motor_CS. IPR001752. Kinesin_motor_dom. IPR027417. P-loop_NTPase. [Graphical view]
Pfam	PF00225. Kinesin. 2 hits. [Graphical view]
PRINTS	PR00380. KINESINHEAVY.
SMART	SM00129. KISc. 1 hit. [Graphical view]
SUPFAM	SSF52540. SSF52540. 1 hit.
PROSITE	PS00411. KINESIN_MOTOR_DOMAIN1. 1 hit. PS50067. KINESIN_MOTOR_DOMAIN2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	982622.

Entry information

Entry name

CIN8_YEAST

Accession

Primary (citable) accession number: P27895
Secondary accession number(s): D3DLI9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	January 10, 2006
Last modified:	May 29, 2013
This is version 121 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program