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P27895 (CIN8_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 132. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Kinesin-like protein CIN8
Alternative name(s):
Chromosome instability protein 8
Gene names
Name:CIN8
Synonyms:KSL2
Ordered Locus Names:YEL061C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1000 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for assembly of the mitotic spindle. Interacts with spindle microtubules to produce an outwardly directed force acting upon the poles. Following spindle assembly, CIN8 and KIP1 apparently act to oppose a force that draws separated poles back together. This force seems to be mediated by KAR3. Ref.1 Ref.5

Subcellular location

Cytoplasmcytoskeletonspindle. Mitochondrion. Note: Spindle microtubules that lie between the poles. Ref.1 Ref.7 Ref.9

Miscellaneous

Present with 238 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. BimC subfamily.

Contains 1 kinesin motor domain.

Sequence caution

The sequence AAA34496.1 differs from that shown. Reason: Erroneous initiation.

The sequence AAB65026.1 differs from that shown. Reason: Erroneous initiation.

The sequence CAA77885.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processCell cycle
Cell division
Mitosis
   Cellular componentCytoplasm
Cytoskeleton
Microtubule
Mitochondrion
   DomainCoiled coil
   LigandATP-binding
Nucleotide-binding
   Molecular functionMotor protein
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processmetabolic process

Inferred from direct assay PubMed 21350123. Source: GOC

microtubule depolymerization

Inferred from mutant phenotype PubMed 19041752. Source: SGD

microtubule-based movement

Inferred from electronic annotation. Source: InterPro

mitotic sister chromatid segregation

Inferred from genetic interaction PubMed 7860634. Source: SGD

mitotic spindle elongation

Inferred from mutant phenotype PubMed 23851487PubMed 9813090. Source: SGD

spindle assembly involved in mitosis

Inferred from mutant phenotype PubMed 23851487PubMed 9813090. Source: SGD

spindle pole body separation

Inferred from mutant phenotype Ref.1. Source: SGD

   Cellular_componentastral microtubule

Inferred from mutant phenotype PubMed 19041752. Source: SGD

condensed nuclear chromosome kinetochore

Inferred from direct assay PubMed 11511347. Source: SGD

kinesin complex

Inferred from electronic annotation. Source: InterPro

kinetochore microtubule

Inferred from direct assay PubMed 19041752. Source: SGD

mitochondrion

Inferred from electronic annotation. Source: UniProtKB-SubCell

spindle microtubule

Inferred from direct assay PubMed 11511347Ref.1. Source: SGD

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

microtubule motor activity

Inferred from genetic interaction PubMed 1618910. Source: SGD

minus-end-directed microtubule motor activity

Inferred from direct assay PubMed 21350123. Source: SGD

plus-end-directed microtubule motor activity

Inferred from direct assay PubMed 21350123. Source: SGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10001000Kinesin-like protein CIN8
PRO_0000125367

Regions

Domain36 – 477442Kinesin motor
Nucleotide binding128 – 1358ATP By similarity
Coiled coil518 – 61598 Potential
Coiled coil860 – 90445 Potential

Amino acid modifications

Modified residue9721Phosphoserine Ref.10

Experimental info

Sequence conflict2161D → A in AAA34496. Ref.1
Sequence conflict2161D → A in CAA77885. Ref.1
Sequence conflict7931Q → H in AAA34496. Ref.1
Sequence conflict7931Q → H in CAA77885. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P27895 [UniParc].

Last modified January 10, 2006. Version 3.
Checksum: B33141C45748CE67

FASTA1,000113,311
        10         20         30         40         50         60 
MPAENQNTGQ DRSSNSISKN GNSQVGCHTV PNEELNITVA VRCRGRNERE ISMKSSVVVN 

        70         80         90        100        110        120 
VPDITGSKEI SINTTGDTGI TAQMNAKRYT VDKVFGPGAS QDLIFDEVAG PLFQDFIKGY 

       130        140        150        160        170        180 
NCTVLVYGMT STGKTYTMTG DEKLYNGELS DAAGIIPRVL LKLFDTLELQ QNDYVVKCSF 

       190        200        210        220        230        240 
IELYNEELKD LLDSNSNGSS NTGFDGQFMK KLRIFDSSTA NNTTSNSASS SRSNSRNSSP 

       250        260        270        280        290        300 
RSLNDLTPKA ALLRKRLRTK SLPNTIKQQY QQQQAVNSRN NSSSNSGSTT NNASSNTNTN 

       310        320        330        340        350        360 
NGQRSSMAPN DQTNGIYIQN LQEFHITNAM EGLNLLQKGL KHRQVASTKM NDFSSRSHTI 

       370        380        390        400        410        420 
FTITLYKKHQ DELFRISKMN LVDLAGSENI NRSGALNQRA KEAGSINQSL LTLGRVINAL 

       430        440        450        460        470        480 
VDKSGHIPFR ESKLTRLLQD SLGGNTKTAL IATISPAKVT SEETCSTLEY ASKAKNIKNK 

       490        500        510        520        530        540 
PQLGSFIMKD ILVKNITMEL AKIKSDLLST KSKEGIYMSQ DHYKNLNSDL ESYKNEVQEC 

       550        560        570        580        590        600 
KREIESLTSK NALLVKDKLK SKETIQSQNC QIESLKTTID HLRAQLDKQH KTEIEISDFN 

       610        620        630        640        650        660 
NKLQKLTEVM QMALHDYKKR ELDLNQKFEM HITKEIKKLK STLFLQLNTM QQESILQETN 

       670        680        690        700        710        720 
IQPNLDMIKN EVLTLMRTMQ EKAELMYKDC VKKILNESPK FFNVVIEKID IIRVDFQKFY 

       730        740        750        760        770        780 
KNIAENLSDI SEENNNMKQY LKNHFFKNNH QELLNRHVDS TYENIEKRTN EFVENFKKVL 

       790        800        810        820        830        840 
NDHLDENKKL IMQNLTTATS AVIDQEMDLF EPKRVKWENS FDLINDCDSM NNEFYNSMAA 

       850        860        870        880        890        900 
TLSQIKSTVD TSSNSMNESI SVMKGQVEES ENAISLLKNN TKFNDQFEQL INKHNMLKDN 

       910        920        930        940        950        960 
IKNSITSTHS HITNVDDIYN TIENIMKNYG NKENATKDEM IENILKEIPN LSKKMPLRLS 

       970        980        990       1000 
NINSNSVQSV ISPKKHAIED ENKSSENVDN EGSRKMLKIE 

« Hide

References

« Hide 'large scale' references
[1]"Two Saccharomyces cerevisiae kinesin-related gene products required for mitotic spindle assembly."
Hoyt M.A., He L., Loo K.K., Saunders W.S.
J. Cell Biol. 118:109-120(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
Strain: ATCC 204508 / S288c.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome V."
Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. expand/collapse author list , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.
Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"A second nitrogen permease regulator in Saccharomyces cerevisiae."
Rousselet G., Simon M., Ripoche P., Buhler J.-M.
FEBS Lett. 359:215-219(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 983-1000.
Strain: ATCC 204508 / S288c.
[5]"Kinesin-related proteins required for structural integrity of the mitotic spindle."
Saunders W.S., Hoyt M.A.
Cell 70:451-458(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
Strain: ATCC 204508 / S288c.
[6]"Sequencing and comparison of yeast species to identify genes and regulatory elements."
Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.
Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
[7]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"The proteome of Saccharomyces cerevisiae mitochondria."
Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C.
Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Strain: ATCC 76625 / YPH499.
[10]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-972, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M90522 Genomic DNA. Translation: AAA34496.1. Different initiation.
Z11859 Genomic DNA. Translation: CAA77885.1. Different initiation.
U18795 Genomic DNA. Translation: AAB65026.1. Different initiation.
X79105 Genomic DNA. Translation: CAA55722.1.
BK006939 Genomic DNA. Translation: DAA07593.1.
PIRB42641. S50528.
RefSeqNP_010853.2. NM_001178876.1.

3D structure databases

ProteinModelPortalP27895.
SMRP27895. Positions 36-486.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36668. 251 interactions.
DIPDIP-3004N.
IntActP27895. 8 interactions.
MINTMINT-435854.
STRING4932.YEL061C.

Proteomic databases

MaxQBP27895.
PaxDbP27895.
PRIDEP27895.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYEL061C; YEL061C; YEL061C.
GeneID856648.
KEGGsce:YEL061C.

Organism-specific databases

CYGDYEL061c.
SGDS000000787. CIN8.

Phylogenomic databases

eggNOGCOG5059.
GeneTreeENSGT00750000117462.
HOGENOMHOG000248614.
OMAQVASTKM.
OrthoDBEOG7RRFGF.

Enzyme and pathway databases

BioCycYEAST:G3O-30176-MONOMER.

Gene expression databases

GenevestigatorP27895.

Family and domain databases

Gene3D3.40.850.10. 2 hits.
InterProIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERPTHR24115. PTHR24115. 1 hit.
PfamPF00225. Kinesin. 2 hits.
[Graphical view]
PRINTSPR00380. KINESINHEAVY.
SMARTSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 2 hits.
PROSITEPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio982622.

Entry information

Entry nameCIN8_YEAST
AccessionPrimary (citable) accession number: P27895
Secondary accession number(s): D3DLI9
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: January 10, 2006
Last modified: June 11, 2014
This is version 132 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families