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Protein

Kinesin-like protein CIN8

Gene

CIN8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for assembly of the mitotic spindle. Interacts with spindle microtubules to produce an outwardly directed force acting upon the poles. Following spindle assembly, CIN8 and KIP1 apparently act to oppose a force that draws separated poles back together. This force seems to be mediated by KAR3.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi128 – 1358ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ATP-dependent microtubule motor activity, minus-end-directed Source: SGD
  3. ATP-dependent microtubule motor activity, plus-end-directed Source: SGD
  4. microtubule motor activity Source: SGD

GO - Biological processi

  1. cell division Source: UniProtKB-KW
  2. metabolic process Source: GOC
  3. microtubule-based movement Source: InterPro
  4. microtubule depolymerization Source: SGD
  5. mitotic sister chromatid segregation Source: SGD
  6. mitotic spindle assembly Source: SGD
  7. mitotic spindle elongation Source: SGD
  8. spindle pole body separation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Motor protein

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30176-MONOMER.
ReactomeiREACT_348362. Kinesins.

Names & Taxonomyi

Protein namesi
Recommended name:
Kinesin-like protein CIN8
Alternative name(s):
Chromosome instability protein 8
Gene namesi
Name:CIN8
Synonyms:KSL2
Ordered Locus Names:YEL061C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome V

Organism-specific databases

CYGDiYEL061c.
EuPathDBiFungiDB:YEL061C.
SGDiS000000787. CIN8.

Subcellular locationi

  1. Cytoplasmcytoskeletonspindle
  2. Mitochondrion

  3. Note: Spindle microtubules that lie between the poles.

GO - Cellular componenti

  1. astral microtubule Source: SGD
  2. condensed nuclear chromosome kinetochore Source: SGD
  3. kinesin complex Source: InterPro
  4. kinetochore microtubule Source: SGD
  5. mitochondrion Source: UniProtKB-SubCell
  6. spindle microtubule Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10001000Kinesin-like protein CIN8PRO_0000125367Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei972 – 9721Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP27895.
PaxDbiP27895.
PRIDEiP27895.

Expressioni

Gene expression databases

GenevestigatoriP27895.

Interactioni

Protein-protein interaction databases

BioGridi36668. 251 interactions.
DIPiDIP-3004N.
IntActiP27895. 8 interactions.
MINTiMINT-435854.
STRINGi4932.YEL061C.

Structurei

3D structure databases

ProteinModelPortaliP27895.
SMRiP27895. Positions 36-223, 315-483.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini36 – 477442Kinesin motorPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili518 – 61598Sequence AnalysisAdd
BLAST
Coiled coili860 – 90445Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. BimC subfamily.PROSITE-ProRule annotation
Contains 1 kinesin motor domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG5059.
GeneTreeiENSGT00770000120453.
HOGENOMiHOG000248614.
InParanoidiP27895.
OMAiQVASTKM.
OrthoDBiEOG7RRFGF.

Family and domain databases

Gene3Di3.40.850.10. 2 hits.
InterProiIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 1 hit.
PfamiPF00225. Kinesin. 2 hits.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P27895-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPAENQNTGQ DRSSNSISKN GNSQVGCHTV PNEELNITVA VRCRGRNERE
60 70 80 90 100
ISMKSSVVVN VPDITGSKEI SINTTGDTGI TAQMNAKRYT VDKVFGPGAS
110 120 130 140 150
QDLIFDEVAG PLFQDFIKGY NCTVLVYGMT STGKTYTMTG DEKLYNGELS
160 170 180 190 200
DAAGIIPRVL LKLFDTLELQ QNDYVVKCSF IELYNEELKD LLDSNSNGSS
210 220 230 240 250
NTGFDGQFMK KLRIFDSSTA NNTTSNSASS SRSNSRNSSP RSLNDLTPKA
260 270 280 290 300
ALLRKRLRTK SLPNTIKQQY QQQQAVNSRN NSSSNSGSTT NNASSNTNTN
310 320 330 340 350
NGQRSSMAPN DQTNGIYIQN LQEFHITNAM EGLNLLQKGL KHRQVASTKM
360 370 380 390 400
NDFSSRSHTI FTITLYKKHQ DELFRISKMN LVDLAGSENI NRSGALNQRA
410 420 430 440 450
KEAGSINQSL LTLGRVINAL VDKSGHIPFR ESKLTRLLQD SLGGNTKTAL
460 470 480 490 500
IATISPAKVT SEETCSTLEY ASKAKNIKNK PQLGSFIMKD ILVKNITMEL
510 520 530 540 550
AKIKSDLLST KSKEGIYMSQ DHYKNLNSDL ESYKNEVQEC KREIESLTSK
560 570 580 590 600
NALLVKDKLK SKETIQSQNC QIESLKTTID HLRAQLDKQH KTEIEISDFN
610 620 630 640 650
NKLQKLTEVM QMALHDYKKR ELDLNQKFEM HITKEIKKLK STLFLQLNTM
660 670 680 690 700
QQESILQETN IQPNLDMIKN EVLTLMRTMQ EKAELMYKDC VKKILNESPK
710 720 730 740 750
FFNVVIEKID IIRVDFQKFY KNIAENLSDI SEENNNMKQY LKNHFFKNNH
760 770 780 790 800
QELLNRHVDS TYENIEKRTN EFVENFKKVL NDHLDENKKL IMQNLTTATS
810 820 830 840 850
AVIDQEMDLF EPKRVKWENS FDLINDCDSM NNEFYNSMAA TLSQIKSTVD
860 870 880 890 900
TSSNSMNESI SVMKGQVEES ENAISLLKNN TKFNDQFEQL INKHNMLKDN
910 920 930 940 950
IKNSITSTHS HITNVDDIYN TIENIMKNYG NKENATKDEM IENILKEIPN
960 970 980 990 1000
LSKKMPLRLS NINSNSVQSV ISPKKHAIED ENKSSENVDN EGSRKMLKIE
Length:1,000
Mass (Da):113,311
Last modified:January 10, 2006 - v3
Checksum:iB33141C45748CE67
GO

Sequence cautioni

The sequence AAA34496.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAB65026.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAA77885.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti216 – 2161D → A in AAA34496 (PubMed:1618897).Curated
Sequence conflicti216 – 2161D → A in CAA77885 (PubMed:1618897).Curated
Sequence conflicti793 – 7931Q → H in AAA34496 (PubMed:1618897).Curated
Sequence conflicti793 – 7931Q → H in CAA77885 (PubMed:1618897).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M90522 Genomic DNA. Translation: AAA34496.1. Different initiation.
Z11859 Genomic DNA. Translation: CAA77885.1. Different initiation.
U18795 Genomic DNA. Translation: AAB65026.1. Different initiation.
X79105 Genomic DNA. Translation: CAA55722.1.
BK006939 Genomic DNA. Translation: DAA07593.1.
PIRiS50528. B42641.
RefSeqiNP_010853.2. NM_001178876.1.

Genome annotation databases

EnsemblFungiiYEL061C; YEL061C; YEL061C.
GeneIDi856648.
KEGGisce:YEL061C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M90522 Genomic DNA. Translation: AAA34496.1. Different initiation.
Z11859 Genomic DNA. Translation: CAA77885.1. Different initiation.
U18795 Genomic DNA. Translation: AAB65026.1. Different initiation.
X79105 Genomic DNA. Translation: CAA55722.1.
BK006939 Genomic DNA. Translation: DAA07593.1.
PIRiS50528. B42641.
RefSeqiNP_010853.2. NM_001178876.1.

3D structure databases

ProteinModelPortaliP27895.
SMRiP27895. Positions 36-223, 315-483.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36668. 251 interactions.
DIPiDIP-3004N.
IntActiP27895. 8 interactions.
MINTiMINT-435854.
STRINGi4932.YEL061C.

Proteomic databases

MaxQBiP27895.
PaxDbiP27895.
PRIDEiP27895.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYEL061C; YEL061C; YEL061C.
GeneIDi856648.
KEGGisce:YEL061C.

Organism-specific databases

CYGDiYEL061c.
EuPathDBiFungiDB:YEL061C.
SGDiS000000787. CIN8.

Phylogenomic databases

eggNOGiCOG5059.
GeneTreeiENSGT00770000120453.
HOGENOMiHOG000248614.
InParanoidiP27895.
OMAiQVASTKM.
OrthoDBiEOG7RRFGF.

Enzyme and pathway databases

BioCyciYEAST:G3O-30176-MONOMER.
ReactomeiREACT_348362. Kinesins.

Miscellaneous databases

NextBioi982622.
PROiP27895.

Gene expression databases

GenevestigatoriP27895.

Family and domain databases

Gene3Di3.40.850.10. 2 hits.
InterProiIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 1 hit.
PfamiPF00225. Kinesin. 2 hits.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Two Saccharomyces cerevisiae kinesin-related gene products required for mitotic spindle assembly."
    Hoyt M.A., He L., Loo K.K., Saunders W.S.
    J. Cell Biol. 118:109-120(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
    Strain: ATCC 204508 / S288c.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "A second nitrogen permease regulator in Saccharomyces cerevisiae."
    Rousselet G., Simon M., Ripoche P., Buhler J.-M.
    FEBS Lett. 359:215-219(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 983-1000.
    Strain: ATCC 204508 / S288c.
  5. "Kinesin-related proteins required for structural integrity of the mitotic spindle."
    Saunders W.S., Hoyt M.A.
    Cell 70:451-458(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: ATCC 204508 / S288c.
  6. "Sequencing and comparison of yeast species to identify genes and regulatory elements."
    Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.
    Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: ATCC 76625 / YPH499.
  10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-972, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCIN8_YEAST
AccessioniPrimary (citable) accession number: P27895
Secondary accession number(s): D3DLI9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: January 10, 2006
Last modified: April 29, 2015
This is version 140 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 238 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.