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P27895

- CIN8_YEAST

UniProt

P27895 - CIN8_YEAST

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Protein
Kinesin-like protein CIN8
Gene
CIN8, KSL2, YEL061C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Required for assembly of the mitotic spindle. Interacts with spindle microtubules to produce an outwardly directed force acting upon the poles. Following spindle assembly, CIN8 and KIP1 apparently act to oppose a force that draws separated poles back together. This force seems to be mediated by KAR3.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi128 – 1358ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. microtubule motor activity Source: SGD
  3. minus-end-directed microtubule motor activity Source: SGD
  4. plus-end-directed microtubule motor activity Source: SGD

GO - Biological processi

  1. metabolic process Source: GOC
  2. microtubule depolymerization Source: SGD
  3. microtubule-based movement Source: InterPro
  4. mitotic sister chromatid segregation Source: SGD
  5. mitotic spindle elongation Source: SGD
  6. spindle assembly involved in mitosis Source: SGD
  7. spindle pole body separation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Motor protein

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30176-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Kinesin-like protein CIN8
Alternative name(s):
Chromosome instability protein 8
Gene namesi
Name:CIN8
Synonyms:KSL2
Ordered Locus Names:YEL061C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

CYGDiYEL061c.
SGDiS000000787. CIN8.

Subcellular locationi

Cytoplasmcytoskeletonspindle. Mitochondrion
Note: Spindle microtubules that lie between the poles.3 Publications

GO - Cellular componenti

  1. astral microtubule Source: SGD
  2. condensed nuclear chromosome kinetochore Source: SGD
  3. kinesin complex Source: InterPro
  4. kinetochore microtubule Source: SGD
  5. mitochondrion Source: UniProtKB-SubCell
  6. spindle microtubule Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10001000Kinesin-like protein CIN8
PRO_0000125367Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei972 – 9721Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP27895.
PaxDbiP27895.
PRIDEiP27895.

Expressioni

Gene expression databases

GenevestigatoriP27895.

Interactioni

Protein-protein interaction databases

BioGridi36668. 251 interactions.
DIPiDIP-3004N.
IntActiP27895. 8 interactions.
MINTiMINT-435854.
STRINGi4932.YEL061C.

Structurei

3D structure databases

ProteinModelPortaliP27895.
SMRiP27895. Positions 36-486.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini36 – 477442Kinesin motor
Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili518 – 61598 Reviewed prediction
Add
BLAST
Coiled coili860 – 90445 Reviewed prediction
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG5059.
GeneTreeiENSGT00750000117462.
HOGENOMiHOG000248614.
OMAiQVASTKM.
OrthoDBiEOG7RRFGF.

Family and domain databases

Gene3Di3.40.850.10. 2 hits.
InterProiIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 1 hit.
PfamiPF00225. Kinesin. 2 hits.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P27895-1 [UniParc]FASTAAdd to Basket

« Hide

MPAENQNTGQ DRSSNSISKN GNSQVGCHTV PNEELNITVA VRCRGRNERE     50
ISMKSSVVVN VPDITGSKEI SINTTGDTGI TAQMNAKRYT VDKVFGPGAS 100
QDLIFDEVAG PLFQDFIKGY NCTVLVYGMT STGKTYTMTG DEKLYNGELS 150
DAAGIIPRVL LKLFDTLELQ QNDYVVKCSF IELYNEELKD LLDSNSNGSS 200
NTGFDGQFMK KLRIFDSSTA NNTTSNSASS SRSNSRNSSP RSLNDLTPKA 250
ALLRKRLRTK SLPNTIKQQY QQQQAVNSRN NSSSNSGSTT NNASSNTNTN 300
NGQRSSMAPN DQTNGIYIQN LQEFHITNAM EGLNLLQKGL KHRQVASTKM 350
NDFSSRSHTI FTITLYKKHQ DELFRISKMN LVDLAGSENI NRSGALNQRA 400
KEAGSINQSL LTLGRVINAL VDKSGHIPFR ESKLTRLLQD SLGGNTKTAL 450
IATISPAKVT SEETCSTLEY ASKAKNIKNK PQLGSFIMKD ILVKNITMEL 500
AKIKSDLLST KSKEGIYMSQ DHYKNLNSDL ESYKNEVQEC KREIESLTSK 550
NALLVKDKLK SKETIQSQNC QIESLKTTID HLRAQLDKQH KTEIEISDFN 600
NKLQKLTEVM QMALHDYKKR ELDLNQKFEM HITKEIKKLK STLFLQLNTM 650
QQESILQETN IQPNLDMIKN EVLTLMRTMQ EKAELMYKDC VKKILNESPK 700
FFNVVIEKID IIRVDFQKFY KNIAENLSDI SEENNNMKQY LKNHFFKNNH 750
QELLNRHVDS TYENIEKRTN EFVENFKKVL NDHLDENKKL IMQNLTTATS 800
AVIDQEMDLF EPKRVKWENS FDLINDCDSM NNEFYNSMAA TLSQIKSTVD 850
TSSNSMNESI SVMKGQVEES ENAISLLKNN TKFNDQFEQL INKHNMLKDN 900
IKNSITSTHS HITNVDDIYN TIENIMKNYG NKENATKDEM IENILKEIPN 950
LSKKMPLRLS NINSNSVQSV ISPKKHAIED ENKSSENVDN EGSRKMLKIE 1000
Length:1,000
Mass (Da):113,311
Last modified:January 10, 2006 - v3
Checksum:iB33141C45748CE67
GO

Sequence cautioni

The sequence AAA34496.1 differs from that shown. Reason: Erroneous initiation.
The sequence AAB65026.1 differs from that shown. Reason: Erroneous initiation.
The sequence CAA77885.1 differs from that shown. Reason: Erroneous initiation.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti216 – 2161D → A in AAA34496. 1 Publication
Sequence conflicti216 – 2161D → A in CAA77885. 1 Publication
Sequence conflicti793 – 7931Q → H in AAA34496. 1 Publication
Sequence conflicti793 – 7931Q → H in CAA77885. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M90522 Genomic DNA. Translation: AAA34496.1. Different initiation.
Z11859 Genomic DNA. Translation: CAA77885.1. Different initiation.
U18795 Genomic DNA. Translation: AAB65026.1. Different initiation.
X79105 Genomic DNA. Translation: CAA55722.1.
BK006939 Genomic DNA. Translation: DAA07593.1.
PIRiS50528. B42641.
RefSeqiNP_010853.2. NM_001178876.1.

Genome annotation databases

EnsemblFungiiYEL061C; YEL061C; YEL061C.
GeneIDi856648.
KEGGisce:YEL061C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M90522 Genomic DNA. Translation: AAA34496.1 . Different initiation.
Z11859 Genomic DNA. Translation: CAA77885.1 . Different initiation.
U18795 Genomic DNA. Translation: AAB65026.1 . Different initiation.
X79105 Genomic DNA. Translation: CAA55722.1 .
BK006939 Genomic DNA. Translation: DAA07593.1 .
PIRi S50528. B42641.
RefSeqi NP_010853.2. NM_001178876.1.

3D structure databases

ProteinModelPortali P27895.
SMRi P27895. Positions 36-486.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36668. 251 interactions.
DIPi DIP-3004N.
IntActi P27895. 8 interactions.
MINTi MINT-435854.
STRINGi 4932.YEL061C.

Proteomic databases

MaxQBi P27895.
PaxDbi P27895.
PRIDEi P27895.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YEL061C ; YEL061C ; YEL061C .
GeneIDi 856648.
KEGGi sce:YEL061C.

Organism-specific databases

CYGDi YEL061c.
SGDi S000000787. CIN8.

Phylogenomic databases

eggNOGi COG5059.
GeneTreei ENSGT00750000117462.
HOGENOMi HOG000248614.
OMAi QVASTKM.
OrthoDBi EOG7RRFGF.

Enzyme and pathway databases

BioCyci YEAST:G3O-30176-MONOMER.

Miscellaneous databases

NextBioi 982622.

Gene expression databases

Genevestigatori P27895.

Family and domain databases

Gene3Di 3.40.850.10. 2 hits.
InterProi IPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view ]
PANTHERi PTHR24115. PTHR24115. 1 hit.
Pfami PF00225. Kinesin. 2 hits.
[Graphical view ]
PRINTSi PR00380. KINESINHEAVY.
SMARTi SM00129. KISc. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 2 hits.
PROSITEi PS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Two Saccharomyces cerevisiae kinesin-related gene products required for mitotic spindle assembly."
    Hoyt M.A., He L., Loo K.K., Saunders W.S.
    J. Cell Biol. 118:109-120(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
    Strain: ATCC 204508 / S288c.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "A second nitrogen permease regulator in Saccharomyces cerevisiae."
    Rousselet G., Simon M., Ripoche P., Buhler J.-M.
    FEBS Lett. 359:215-219(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 983-1000.
    Strain: ATCC 204508 / S288c.
  5. "Kinesin-related proteins required for structural integrity of the mitotic spindle."
    Saunders W.S., Hoyt M.A.
    Cell 70:451-458(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: ATCC 204508 / S288c.
  6. "Sequencing and comparison of yeast species to identify genes and regulatory elements."
    Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.
    Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: ATCC 76625 / YPH499.
  10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-972, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCIN8_YEAST
AccessioniPrimary (citable) accession number: P27895
Secondary accession number(s): D3DLI9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: January 10, 2006
Last modified: June 11, 2014
This is version 132 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 238 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3

Similar proteinsi