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P27895

- CIN8_YEAST

UniProt

P27895 - CIN8_YEAST

Protein

Kinesin-like protein CIN8

Gene

CIN8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 133 (01 Oct 2014)
      Sequence version 3 (10 Jan 2006)
      Previous versions | rss
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    Functioni

    Required for assembly of the mitotic spindle. Interacts with spindle microtubules to produce an outwardly directed force acting upon the poles. Following spindle assembly, CIN8 and KIP1 apparently act to oppose a force that draws separated poles back together. This force seems to be mediated by KAR3.2 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi128 – 1358ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. microtubule motor activity Source: SGD
    3. minus-end-directed microtubule motor activity Source: SGD
    4. plus-end-directed microtubule motor activity Source: SGD

    GO - Biological processi

    1. metabolic process Source: GOC
    2. microtubule-based movement Source: InterPro
    3. microtubule depolymerization Source: SGD
    4. mitotic sister chromatid segregation Source: SGD
    5. mitotic spindle elongation Source: SGD
    6. spindle assembly involved in mitosis Source: SGD
    7. spindle pole body separation Source: SGD

    Keywords - Molecular functioni

    Motor protein

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30176-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Kinesin-like protein CIN8
    Alternative name(s):
    Chromosome instability protein 8
    Gene namesi
    Name:CIN8
    Synonyms:KSL2
    Ordered Locus Names:YEL061C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome V

    Organism-specific databases

    CYGDiYEL061c.
    SGDiS000000787. CIN8.

    Subcellular locationi

    Cytoplasmcytoskeletonspindle. Mitochondrion
    Note: Spindle microtubules that lie between the poles.

    GO - Cellular componenti

    1. astral microtubule Source: SGD
    2. condensed nuclear chromosome kinetochore Source: SGD
    3. kinesin complex Source: InterPro
    4. kinetochore microtubule Source: SGD
    5. mitochondrion Source: UniProtKB-SubCell
    6. spindle microtubule Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Microtubule, Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10001000Kinesin-like protein CIN8PRO_0000125367Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei972 – 9721Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP27895.
    PaxDbiP27895.
    PRIDEiP27895.

    Expressioni

    Gene expression databases

    GenevestigatoriP27895.

    Interactioni

    Protein-protein interaction databases

    BioGridi36668. 251 interactions.
    DIPiDIP-3004N.
    IntActiP27895. 8 interactions.
    MINTiMINT-435854.
    STRINGi4932.YEL061C.

    Structurei

    3D structure databases

    ProteinModelPortaliP27895.
    SMRiP27895. Positions 36-486.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini36 – 477442Kinesin motorPROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili518 – 61598Sequence AnalysisAdd
    BLAST
    Coiled coili860 – 90445Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. BimC subfamily.PROSITE-ProRule annotation
    Contains 1 kinesin motor domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiCOG5059.
    GeneTreeiENSGT00750000117462.
    HOGENOMiHOG000248614.
    OMAiQVASTKM.
    OrthoDBiEOG7RRFGF.

    Family and domain databases

    Gene3Di3.40.850.10. 2 hits.
    InterProiIPR027640. Kinesin-like_fam.
    IPR019821. Kinesin_motor_CS.
    IPR001752. Kinesin_motor_dom.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PANTHERiPTHR24115. PTHR24115. 1 hit.
    PfamiPF00225. Kinesin. 2 hits.
    [Graphical view]
    PRINTSiPR00380. KINESINHEAVY.
    SMARTiSM00129. KISc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 2 hits.
    PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
    PS50067. KINESIN_MOTOR_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P27895-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPAENQNTGQ DRSSNSISKN GNSQVGCHTV PNEELNITVA VRCRGRNERE     50
    ISMKSSVVVN VPDITGSKEI SINTTGDTGI TAQMNAKRYT VDKVFGPGAS 100
    QDLIFDEVAG PLFQDFIKGY NCTVLVYGMT STGKTYTMTG DEKLYNGELS 150
    DAAGIIPRVL LKLFDTLELQ QNDYVVKCSF IELYNEELKD LLDSNSNGSS 200
    NTGFDGQFMK KLRIFDSSTA NNTTSNSASS SRSNSRNSSP RSLNDLTPKA 250
    ALLRKRLRTK SLPNTIKQQY QQQQAVNSRN NSSSNSGSTT NNASSNTNTN 300
    NGQRSSMAPN DQTNGIYIQN LQEFHITNAM EGLNLLQKGL KHRQVASTKM 350
    NDFSSRSHTI FTITLYKKHQ DELFRISKMN LVDLAGSENI NRSGALNQRA 400
    KEAGSINQSL LTLGRVINAL VDKSGHIPFR ESKLTRLLQD SLGGNTKTAL 450
    IATISPAKVT SEETCSTLEY ASKAKNIKNK PQLGSFIMKD ILVKNITMEL 500
    AKIKSDLLST KSKEGIYMSQ DHYKNLNSDL ESYKNEVQEC KREIESLTSK 550
    NALLVKDKLK SKETIQSQNC QIESLKTTID HLRAQLDKQH KTEIEISDFN 600
    NKLQKLTEVM QMALHDYKKR ELDLNQKFEM HITKEIKKLK STLFLQLNTM 650
    QQESILQETN IQPNLDMIKN EVLTLMRTMQ EKAELMYKDC VKKILNESPK 700
    FFNVVIEKID IIRVDFQKFY KNIAENLSDI SEENNNMKQY LKNHFFKNNH 750
    QELLNRHVDS TYENIEKRTN EFVENFKKVL NDHLDENKKL IMQNLTTATS 800
    AVIDQEMDLF EPKRVKWENS FDLINDCDSM NNEFYNSMAA TLSQIKSTVD 850
    TSSNSMNESI SVMKGQVEES ENAISLLKNN TKFNDQFEQL INKHNMLKDN 900
    IKNSITSTHS HITNVDDIYN TIENIMKNYG NKENATKDEM IENILKEIPN 950
    LSKKMPLRLS NINSNSVQSV ISPKKHAIED ENKSSENVDN EGSRKMLKIE 1000
    Length:1,000
    Mass (Da):113,311
    Last modified:January 10, 2006 - v3
    Checksum:iB33141C45748CE67
    GO

    Sequence cautioni

    The sequence AAA34496.1 differs from that shown. Reason: Erroneous initiation.
    The sequence AAB65026.1 differs from that shown. Reason: Erroneous initiation.
    The sequence CAA77885.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti216 – 2161D → A in AAA34496. (PubMed:1618897)Curated
    Sequence conflicti216 – 2161D → A in CAA77885. (PubMed:1618897)Curated
    Sequence conflicti793 – 7931Q → H in AAA34496. (PubMed:1618897)Curated
    Sequence conflicti793 – 7931Q → H in CAA77885. (PubMed:1618897)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M90522 Genomic DNA. Translation: AAA34496.1. Different initiation.
    Z11859 Genomic DNA. Translation: CAA77885.1. Different initiation.
    U18795 Genomic DNA. Translation: AAB65026.1. Different initiation.
    X79105 Genomic DNA. Translation: CAA55722.1.
    BK006939 Genomic DNA. Translation: DAA07593.1.
    PIRiS50528. B42641.
    RefSeqiNP_010853.2. NM_001178876.1.

    Genome annotation databases

    EnsemblFungiiYEL061C; YEL061C; YEL061C.
    GeneIDi856648.
    KEGGisce:YEL061C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M90522 Genomic DNA. Translation: AAA34496.1 . Different initiation.
    Z11859 Genomic DNA. Translation: CAA77885.1 . Different initiation.
    U18795 Genomic DNA. Translation: AAB65026.1 . Different initiation.
    X79105 Genomic DNA. Translation: CAA55722.1 .
    BK006939 Genomic DNA. Translation: DAA07593.1 .
    PIRi S50528. B42641.
    RefSeqi NP_010853.2. NM_001178876.1.

    3D structure databases

    ProteinModelPortali P27895.
    SMRi P27895. Positions 36-486.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36668. 251 interactions.
    DIPi DIP-3004N.
    IntActi P27895. 8 interactions.
    MINTi MINT-435854.
    STRINGi 4932.YEL061C.

    Proteomic databases

    MaxQBi P27895.
    PaxDbi P27895.
    PRIDEi P27895.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YEL061C ; YEL061C ; YEL061C .
    GeneIDi 856648.
    KEGGi sce:YEL061C.

    Organism-specific databases

    CYGDi YEL061c.
    SGDi S000000787. CIN8.

    Phylogenomic databases

    eggNOGi COG5059.
    GeneTreei ENSGT00750000117462.
    HOGENOMi HOG000248614.
    OMAi QVASTKM.
    OrthoDBi EOG7RRFGF.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30176-MONOMER.

    Miscellaneous databases

    NextBioi 982622.

    Gene expression databases

    Genevestigatori P27895.

    Family and domain databases

    Gene3Di 3.40.850.10. 2 hits.
    InterProi IPR027640. Kinesin-like_fam.
    IPR019821. Kinesin_motor_CS.
    IPR001752. Kinesin_motor_dom.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    PANTHERi PTHR24115. PTHR24115. 1 hit.
    Pfami PF00225. Kinesin. 2 hits.
    [Graphical view ]
    PRINTSi PR00380. KINESINHEAVY.
    SMARTi SM00129. KISc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 2 hits.
    PROSITEi PS00411. KINESIN_MOTOR_1. 1 hit.
    PS50067. KINESIN_MOTOR_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Two Saccharomyces cerevisiae kinesin-related gene products required for mitotic spindle assembly."
      Hoyt M.A., He L., Loo K.K., Saunders W.S.
      J. Cell Biol. 118:109-120(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
      Strain: ATCC 204508 / S288c.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "A second nitrogen permease regulator in Saccharomyces cerevisiae."
      Rousselet G., Simon M., Ripoche P., Buhler J.-M.
      FEBS Lett. 359:215-219(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 983-1000.
      Strain: ATCC 204508 / S288c.
    5. "Kinesin-related proteins required for structural integrity of the mitotic spindle."
      Saunders W.S., Hoyt M.A.
      Cell 70:451-458(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
      Strain: ATCC 204508 / S288c.
    6. "Sequencing and comparison of yeast species to identify genes and regulatory elements."
      Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.
      Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
    7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Strain: ATCC 76625 / YPH499.
    10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-972, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiCIN8_YEAST
    AccessioniPrimary (citable) accession number: P27895
    Secondary accession number(s): D3DLI9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: January 10, 2006
    Last modified: October 1, 2014
    This is version 133 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 238 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome V
      Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

    External Data

    Dasty 3