Reviewed, UniProtKB/Swiss-Prot P27895 (CIN8_YEAST)

Names and origin

Protein names

Recommended name:
    Kinesin-like protein CIN8
Alternative name(s):
    Chromosome instability protein 8

Gene names

Name:	CIN8
Synonyms:	KSL2
Ordered Locus Names:	YEL061C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1000 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required for assembly of the mitotic spindle. Interacts with spindle microtubules to produce an outwardly directed force acting upon the poles. Following spindle assembly, CIN8 and KIP1 apparently act to oppose a force that draws separated poles back together. This force seems to be mediated by KAR3. Ref.1 Ref.4
Subcellular location	Cytoplasm › cytoskeleton › spindle. Mitochondrion. Note: Spindle microtubules that lie between the poles. Ref.1 Ref.6 Ref.8
Miscellaneous	Present with 238 molecules/cell in log phase SD medium. Ref.7
Sequence similarities	Belongs to the kinesin-like protein family. BimC subfamily. Contains 1 kinesin-motor domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Cytoplasm Cytoskeleton Microtubule Mitochondrion
Domain	Coiled coil
Ligand	ATP-binding Nucleotide-binding
Molecular function	Motor protein
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell division Inferred from electronic annotation. Source: UniProtKB-KW microtubule depolymerization Inferred from mutant phenotype. Source: SGD microtubule-based movement Inferred from electronic annotation. Source: InterPro mitotic anaphase B Inferred from mutant phenotype. Source: SGD mitotic sister chromatid segregation Inferred from genetic interaction. Source: SGD mitotic spindle organization in nucleus Traceable author statement. Source: SGD spindle pole body separation Ref.1 Inferred from genetic interaction. Source: SGD
Cellular component	astral microtubule Inferred from mutant phenotype. Source: SGD condensed nuclear chromosome kinetochore Inferred from direct assay. Source: SGD kinesin complex Traceable author statement. Source: SGD kinetochore microtubule Inferred from direct assay. Source: SGD mitochondrion Ref.8 Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW microtubule motor activity Traceable author statement. Source: SGD protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MXR1	P40029	1	EBI-4687,EBI-11441

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1000	1000	Kinesin-like protein CIN8		PRO_0000125367
Regions
	Domain	33 – 408	376	Kinesin-motor
	Nucleotide binding	128 – 135	8	ATP By similarity
	Coiled coil	518 – 615	98	Potential
	Coiled coil	860 – 904	45	Potential
Amino acid modifications
	Modified residue	972	1	Phosphoserine Ref.9
Experimental info
	Sequence conflict	216	1	D → A in AAA34496. Ref.1
	Sequence conflict	216	1	D → A in CAA77885. Ref.3
	Sequence conflict	793	1	Q → H in AAA34496. Ref.1
	Sequence conflict	793	1	Q → H in CAA77885. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P27895-1 [UniParc]. Last modified January 10, 2006. Version 3. Checksum: B33141C45748CE67	FASTA	1,000	113,311
	10 20 30 40 50 60 MPAENQNTGQ DRSSNSISKN GNSQVGCHTV PNEELNITVA VRCRGRNERE ISMKSSVVVN 70 80 90 100 110 120 VPDITGSKEI SINTTGDTGI TAQMNAKRYT VDKVFGPGAS QDLIFDEVAG PLFQDFIKGY 130 140 150 160 170 180 NCTVLVYGMT STGKTYTMTG DEKLYNGELS DAAGIIPRVL LKLFDTLELQ QNDYVVKCSF 190 200 210 220 230 240 IELYNEELKD LLDSNSNGSS NTGFDGQFMK KLRIFDSSTA NNTTSNSASS SRSNSRNSSP 250 260 270 280 290 300 RSLNDLTPKA ALLRKRLRTK SLPNTIKQQY QQQQAVNSRN NSSSNSGSTT NNASSNTNTN 310 320 330 340 350 360 NGQRSSMAPN DQTNGIYIQN LQEFHITNAM EGLNLLQKGL KHRQVASTKM NDFSSRSHTI 370 380 390 400 410 420 FTITLYKKHQ DELFRISKMN LVDLAGSENI NRSGALNQRA KEAGSINQSL LTLGRVINAL 430 440 450 460 470 480 VDKSGHIPFR ESKLTRLLQD SLGGNTKTAL IATISPAKVT SEETCSTLEY ASKAKNIKNK 490 500 510 520 530 540 PQLGSFIMKD ILVKNITMEL AKIKSDLLST KSKEGIYMSQ DHYKNLNSDL ESYKNEVQEC 550 560 570 580 590 600 KREIESLTSK NALLVKDKLK SKETIQSQNC QIESLKTTID HLRAQLDKQH KTEIEISDFN 610 620 630 640 650 660 NKLQKLTEVM QMALHDYKKR ELDLNQKFEM HITKEIKKLK STLFLQLNTM QQESILQETN 670 680 690 700 710 720 IQPNLDMIKN EVLTLMRTMQ EKAELMYKDC VKKILNESPK FFNVVIEKID IIRVDFQKFY 730 740 750 760 770 780 KNIAENLSDI SEENNNMKQY LKNHFFKNNH QELLNRHVDS TYENIEKRTN EFVENFKKVL 790 800 810 820 830 840 NDHLDENKKL IMQNLTTATS AVIDQEMDLF EPKRVKWENS FDLINDCDSM NNEFYNSMAA 850 860 870 880 890 900 TLSQIKSTVD TSSNSMNESI SVMKGQVEES ENAISLLKNN TKFNDQFEQL INKHNMLKDN 910 920 930 940 950 960 IKNSITSTHS HITNVDDIYN TIENIMKNYG NKENATKDEM IENILKEIPN LSKKMPLRLS 970 980 990 1000 NINSNSVQSV ISPKKHAIED ENKSSENVDN EGSRKMLKIE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two Saccharomyces cerevisiae kinesin-related gene products required for mitotic spindle assembly." Hoyt M.A., He L., Loo K.K., Saunders W.S. J. Cell Biol. 118:109-120(1992) [PubMed: 1618897] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"A second nitrogen permease regulator in Saccharomyces cerevisiae." Rousselet G., Simon M., Ripoche P., Buhler J.-M. FEBS Lett. 359:215-219(1995) [PubMed: 7867803] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 983-1000. Strain: ATCC 204508 / S288c.
[4]	"Kinesin-related proteins required for structural integrity of the mitotic spindle." Saunders W.S., Hoyt M.A. Cell 70:451-458(1992) [PubMed: 1643659] [Abstract] Cited for: FUNCTION. Strain: ATCC 204508 / S288c.
[5]	"Sequencing and comparison of yeast species to identify genes and regulatory elements." Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S. Nature 423:241-254(2003) [PubMed: 12748633] [Abstract] Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"The proteome of Saccharomyces cerevisiae mitochondria." Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C. Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed: 14576278] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-972, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z11859 Genomic DNA. Translation: CAA77885.1. Different initiation. M90522 Genomic DNA. Translation: AAA34496.1. Different initiation. U18795 Genomic DNA. Translation: AAB65026.1. Different initiation. X79105 Genomic DNA. Translation: CAA55722.1.
PIR	B42641. S50528.
RefSeq	NP_010853.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:3004N.
IntAct	P27895. 10 interactions.
STRING	P27895.
Proteomic databases
PRIDE	P27895.
Genome annotation databases
Ensembl	YEL061C; YEL061C; YEL061C; Saccharomyces cerevisiae. [Genome view]
GeneID	856648.
KEGG	sce:YEL061C.
NMPDR	fig|4932.3.peg.1901.
Organism-specific databases
CYGD	YEL061c.
SGD	S000000787. CIN8.
Phylogenomic databases
HOGENOM	P27895.
OMA	QVASTKM
OrthoDB	EOG9TB5TK
Gene expression databases
ArrayExpress	P27895.
Genevestigator	P27895.
GermOnline	YEL061C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001752. Kinesin_motor. IPR019821. Kinesin_motor_CS. [Graphical view]
Gene3D	G3DSA:3.40.850.10. kinesin_motor. 1 hit.
Pfam	PF00225. Kinesin. 1 hit. [Graphical view]
PRINTS	PR00380. KINESINHEAVY.
SMART	SM00129. KISc. 1 hit. [Graphical view]
PROSITE	PS00411. KINESIN_MOTOR_DOMAIN1. 1 hit. PS50067. KINESIN_MOTOR_DOMAIN2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	982622.

Entry information

Entry name

CIN8_YEAST

Accession

Primary (citable) accession number: P27895

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	January 10, 2006
Last modified:	November 24, 2009
This is version 87 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)