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P27889 (HNF1B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 139. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hepatocyte nuclear factor 1-beta

Short name=HNF-1-beta
Short name=HNF-1B
Alternative name(s):
Homeoprotein LFB3
Transcription factor 2
Short name=TCF-2
Gene names
Name:Hnf1b
Synonyms:Hnf-1b, Tcf2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length558 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription factor, probably binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.

Subunit structure

Binds DNA as a dimer. Can form homodimer or heterodimer with HNF1-alpha.

Subcellular location

Nucleus.

Sequence similarities

Belongs to the HNF1 homeobox family.

Contains 1 homeobox DNA-binding domain.

Sequence caution

The sequence BAB31632.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainHomeobox
   LigandDNA-binding
   Molecular functionActivator
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processanterior/posterior pattern specification

Inferred from mutant phenotype PubMed 15872003. Source: MGI

branching morphogenesis of an epithelial tube

Inferred from mutant phenotype PubMed 20040500. Source: MGI

embryonic digestive tract morphogenesis

Inferred from mutant phenotype PubMed 18635606. Source: MGI

endocrine pancreas development

Inferred from sequence or structural similarity. Source: UniProtKB

endoderm development

Inferred from mutant phenotype PubMed 10518495PubMed 10518496PubMed 18635606. Source: MGI

endoderm formation

Inferred from sequence orthology PubMed 18635606. Source: MGI

endodermal cell fate specification

Inferred from mutant phenotype PubMed 18635606. Source: MGI

epithelial cell proliferation

Inferred from mutant phenotype PubMed 15647252. Source: MGI

epithelium development

Inferred from mutant phenotype PubMed 18635606. Source: MGI

genitalia development

Inferred from sequence or structural similarity. Source: UniProtKB

hepatoblast differentiation

Inferred from mutant phenotype PubMed 18635606. Source: MGI

hindbrain development

Inferred from mutant phenotype PubMed 15872003. Source: MGI

inner cell mass cell differentiation

Inferred from mutant phenotype PubMed 10518496. Source: MGI

insulin secretion

Inferred from mutant phenotype PubMed 15142986. Source: MGI

kidney development

Inferred from sequence or structural similarity. Source: UniProtKB

kidney morphogenesis

Inferred from genetic interaction PubMed 23362348. Source: MGI

liver development

Inferred from mutant phenotype PubMed 18635606. Source: MGI

mesonephric duct development

Inferred from mutant phenotype PubMed 20040500. Source: MGI

mesonephric duct formation

Inferred from mutant phenotype PubMed 20040500. Source: MGI

mesonephric tubule development

Inferred from genetic interaction PubMed 20040500. Source: MGI

negative regulation of apoptotic process

Inferred from mutant phenotype PubMed 18635606. Source: MGI

negative regulation of mesenchymal cell apoptotic process involved in mesonephric nephron morphogenesis

Inferred from mutant phenotype PubMed 20040500. Source: MGI

negative regulation of mesenchymal cell apoptotic process involved in metanephros development

Inferred from mutant phenotype PubMed 20040500. Source: MGI

negative regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 18635606. Source: MGI

nephric duct development

Inferred from mutant phenotype PubMed 20040500. Source: MGI

nephric duct formation

Inferred from mutant phenotype PubMed 19913010. Source: MGI

positive regulation of gene expression

Inferred from mutant phenotype PubMed 20040500. Source: MGI

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 16274963PubMed 18635606. Source: MGI

positive regulation of transcription initiation from RNA polymerase II promoter

Inferred from electronic annotation. Source: Ensembl

positive regulation of transcription, DNA-templated

Inferred from sequence or structural similarity. Source: UniProtKB

pronephric nephron tubule development

Inferred from electronic annotation. Source: Ensembl

regulation of Wnt signaling pathway

Inferred from mutant phenotype PubMed 20040500. Source: MGI

regulation of branch elongation involved in ureteric bud branching

Inferred from mutant phenotype PubMed 20040500. Source: MGI

regulation of endodermal cell fate specification

Inferred from mutant phenotype PubMed 18635606. Source: MGI

regulation of pronephros size

Inferred from electronic annotation. Source: Ensembl

response to glucose

Inferred from mutant phenotype PubMed 15142986. Source: MGI

transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 20040500. Source: GOC

ureteric bud elongation

Inferred from mutant phenotype PubMed 20040500. Source: MGI

   Cellular_componentnucleoplasm

Traceable author statement. Source: Reactome

nucleus

Inferred from sequence or structural similarity. Source: UniProtKB

transcription factor complex

Inferred from physical interaction PubMed 14570708. Source: MGI

   Molecular_functionDNA binding

Inferred from direct assay PubMed 1363228PubMed 14570708PubMed 15067314PubMed 15509593PubMed 15647252PubMed 16274963Ref.1PubMed 1685988PubMed 16912278PubMed 2044952PubMed 8598044. Source: MGI

RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity

Inferred from direct assay PubMed 20040500. Source: MGI

protein binding

Inferred from physical interaction PubMed 15509593PubMed 15647252PubMed 8598044. Source: MGI

protein heterodimerization activity

Inferred from physical interaction Ref.1PubMed 1685988PubMed 2044952. Source: MGI

protein homodimerization activity

Inferred from sequence or structural similarity. Source: UniProtKB

sequence-specific DNA binding

Inferred from electronic annotation. Source: InterPro

sequence-specific DNA binding transcription factor activity

Inferred from direct assay PubMed 12367519. Source: UniProtKB

transcription regulatory region DNA binding

Inferred from direct assay PubMed 20040500. Source: MGI

Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P27889-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P27889-2)

The sequence of this isoform differs from the canonical sequence as follows:
     183-208: Missing.
Isoform 3 (identifier: P27889-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-123: Missing.
Note: No experimental confirmation available.
Isoform 4 (identifier: P27889-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-123: Missing.
     183-208: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 558558Hepatocyte nuclear factor 1-beta
PRO_0000049122

Regions

DNA binding231 – 31181Homeobox; HNF1-type
Region1 – 3131Dimerization By similarity

Natural variations

Alternative sequence1 – 123123Missing in isoform 3 and isoform 4.
VSP_007099
Alternative sequence183 – 20826Missing in isoform 2 and isoform 4.
VSP_007100
Natural variant2221T → A in strain: NSY. Ref.2 Ref.3

Experimental info

Sequence conflict671N → S in BAB31632. Ref.4
Sequence conflict423 – 4297LSHHNPQ → THSPPQSP in CAA39358. Ref.1
Sequence conflict520 – 5212PP → HT in CAA39358. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified April 4, 2003. Version 2.
Checksum: D4F2BC92B64374A6

FASTA55861,588
        10         20         30         40         50         60 
MVSKLTSLQQ ELLSALLSSG VTKEVLIQAL EELLPSPNFG VKLETLPLSP GSGADLDTKP 

        70         80         90        100        110        120 
VFHTLTNGHA KGRLSGDEGS EDGDDYDTPP ILKELQALNT EEAAEQRAEV DRMLSEDPWR 

       130        140        150        160        170        180 
AAKMIKGYMQ QHNIPQREVV DVTGLNQSHL SQHLNKGTPM KTQKRAALYT WYVRKQREIL 

       190        200        210        220        230        240 
RQFNQTVQSS GNMTDKSSQD QLLFLFPEFS QQNQGPGQSE DTCSEPTNKK MRRNRFKWGP 

       250        260        270        280        290        300 
ASQQILYQAY DRQKNPSKEE REALVEECNR AECLQRGVSP SKAHGLGSNL VTEVRVYNWF 

       310        320        330        340        350        360 
ANRRKEEAFR QKLAMDAYSS NQTHNLNPLL THGSPHHQPS SSPPNKMSGV RYNQPGNNEV 

       370        380        390        400        410        420 
TSSSTISHHG NSAMVTSQSV LQQVSPASLD PGHSLLSPDS KMQITVSGGG LPPVSTLTNI 

       430        440        450        460        470        480 
HSLSHHNPQQ SQNLIMTPLS GVMAIAQSLN TSQAQGVPVI NSVASSLAAL QPVQFSQQLH 

       490        500        510        520        530        540 
SPHQQPLMQQ SPGSHMAQQP FMAAVTQLQN SHMYAHKQEP PQYSHTSRFP SAMVVTDTSS 

       550 
INTLTSMSSS KQCPLQAW 

« Hide

Isoform 2 [UniParc].

Checksum: A7AD8DBD40D40E4F
Show »

FASTA53258,644
Isoform 3 [UniParc].

Checksum: 8885C6A5474F7786
Show »

FASTA43548,336
Isoform 4 [UniParc].

Checksum: 4B8FDE392F0D55CC
Show »

FASTA40945,392

References

« Hide 'large scale' references
[1]"LFB3, a heterodimer-forming homeoprotein of the LFB1 family, is expressed in specialized epithelia."
de Simone V., de Magistris L., Lazzaro L., Gerstner J., Monaci P., Nicosia A., Cortese R.
EMBO J. 10:1435-1443(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Genetic analysis of late-onset type 2 diabetes in a mouse model of human complex trait."
Ueda H., Ikegami H., Kawaguchi Y., Fujisawa T., Yamato E., Shibata M., Ogihara T.
Diabetes 48:1168-1174(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), VARIANT ALA-222.
Strain: C3H and NSY.
Tissue: Kidney.
[3]"Sequence analysis of candidate genes for common susceptibility to type 1 and type 2 diabetes in mice."
Yamada K., Ikegami H., Kawaguchi Y., Fujisawa T., Hotta M., Ueda H., Shintani M., Nojima K., Kawabata Y., Ono M., Nishino M., Itoi M., Babaya N., Shibata M., Makino S., Ogihara T.
Endocr. J. 48:241-247(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT ALA-222.
Strain: C3H, NOD and NSY.
[4]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 4).
Strain: C57BL/6J.
Tissue: Embryo and Liver.
[5]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Tissue: Kidney.
[7]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X55842 mRNA. Translation: CAA39358.1.
AB008174 mRNA. Translation: BAA77718.1.
AB008175 mRNA. Translation: BAA77719.1.
AB008176 mRNA. Translation: BAA77720.1.
AB008177 mRNA. Translation: BAA77721.1.
AB052659 mRNA. Translation: BAB60814.1.
AK004837 mRNA. Translation: BAB23604.1.
AK019258 mRNA. Translation: BAB31632.2. Different initiation.
AL669868 Genomic DNA. Translation: CAI35313.1.
BC025189 mRNA. Translation: AAH25189.1.
CCDSCCDS25179.1. [P27889-1]
PIRA39633.
RefSeqNP_001278197.1. NM_001291268.1. [P27889-2]
NP_001278198.1. NM_001291269.1. [P27889-3]
NP_033356.2. NM_009330.3. [P27889-1]
XP_006532859.1. XM_006532796.1. [P27889-3]
UniGeneMm.7226.

3D structure databases

ProteinModelPortalP27889.
SMRP27889. Positions 91-321.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid204003. 5 interactions.
IntActP27889. 1 interaction.
MINTMINT-4097641.

PTM databases

PhosphoSiteP27889.

Proteomic databases

MaxQBP27889.
PaxDbP27889.
PRIDEP27889.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000021016; ENSMUSP00000021016; ENSMUSG00000020679. [P27889-1]
ENSMUST00000108113; ENSMUSP00000103748; ENSMUSG00000020679. [P27889-3]
ENSMUST00000108114; ENSMUSP00000103749; ENSMUSG00000020679. [P27889-2]
GeneID21410.
KEGGmmu:21410.
UCSCuc007kpx.1. mouse. [P27889-1]

Organism-specific databases

CTD6928.
MGIMGI:98505. Hnf1b.

Phylogenomic databases

eggNOGNOG79356.
GeneTreeENSGT00730000110937.
HOVERGENHBG005980.
InParanoidQ5NC37.
KOK08034.
OMAMYTHKQE.
OrthoDBEOG769ZJ9.
PhylomeDBP27889.
TreeFamTF320327.

Enzyme and pathway databases

ReactomeREACT_13641. Regulation of Beta-Cell Development.
REACT_188576. Developmental Biology.

Gene expression databases

ArrayExpressP27889.
BgeeP27889.
CleanExMM_HNF1B.
GenevestigatorP27889.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProIPR006899. HNF-1_N.
IPR023219. HNF1_dimer_dom.
IPR006897. HNF1b_C.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
[Graphical view]
PfamPF04814. HNF-1_N. 1 hit.
PF04812. HNF-1B_C. 1 hit.
PF00046. Homeobox. 1 hit.
[Graphical view]
SMARTSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMSSF100957. SSF100957. 1 hit.
SSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio300688.
PROP27889.
SOURCESearch...

Entry information

Entry nameHNF1B_MOUSE
AccessionPrimary (citable) accession number: P27889
Secondary accession number(s): Q5NC37 expand/collapse secondary AC list , Q8R162, Q9CS26, Q9R1W1, Q9R1W2, Q9WTL5, Q9WTL6
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: April 4, 2003
Last modified: July 9, 2014
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot