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Protein

Hepatocyte nuclear factor 1-beta

Gene

Hnf1b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor, probably binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi231 – 31181Homeobox; HNF1-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: MGI
  2. protein heterodimerization activity Source: MGI
  3. protein homodimerization activity Source: UniProtKB
  4. RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Source: MGI
  5. sequence-specific DNA binding transcription factor activity Source: UniProtKB
  6. transcription regulatory region DNA binding Source: MGI

GO - Biological processi

  1. anterior/posterior pattern specification Source: MGI
  2. branching morphogenesis of an epithelial tube Source: MGI
  3. embryonic digestive tract morphogenesis Source: MGI
  4. endocrine pancreas development Source: UniProtKB
  5. endodermal cell fate specification Source: MGI
  6. endoderm development Source: MGI
  7. endoderm formation Source: MGI
  8. epithelial cell proliferation Source: MGI
  9. epithelium development Source: MGI
  10. genitalia development Source: UniProtKB
  11. hepatoblast differentiation Source: MGI
  12. hindbrain development Source: MGI
  13. inner cell mass cell differentiation Source: MGI
  14. insulin secretion Source: MGI
  15. kidney development Source: UniProtKB
  16. kidney morphogenesis Source: MGI
  17. liver development Source: MGI
  18. mesonephric duct development Source: MGI
  19. mesonephric duct formation Source: MGI
  20. mesonephric tubule development Source: MGI
  21. negative regulation of apoptotic process Source: MGI
  22. negative regulation of mesenchymal cell apoptotic process involved in mesonephric nephron morphogenesis Source: MGI
  23. negative regulation of mesenchymal cell apoptotic process involved in metanephros development Source: MGI
  24. negative regulation of transcription from RNA polymerase II promoter Source: MGI
  25. nephric duct development Source: MGI
  26. nephric duct formation Source: MGI
  27. positive regulation of gene expression Source: MGI
  28. positive regulation of transcription, DNA-templated Source: UniProtKB
  29. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  30. positive regulation of transcription initiation from RNA polymerase II promoter Source: MGI
  31. pronephric nephron tubule development Source: MGI
  32. pronephros development Source: MGI
  33. regulation of branch elongation involved in ureteric bud branching Source: MGI
  34. regulation of endodermal cell fate specification Source: MGI
  35. regulation of pronephros size Source: MGI
  36. regulation of Wnt signaling pathway Source: MGI
  37. response to glucose Source: MGI
  38. transcription from RNA polymerase II promoter Source: GOC
  39. ureteric bud elongation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_208465. Regulation of gene expression in early pancreatic precursor cells.
REACT_222486. Regulation of gene expression in late stage (branching morphogenesis) pancreatic bud precursor cells.

Names & Taxonomyi

Protein namesi
Recommended name:
Hepatocyte nuclear factor 1-beta
Short name:
HNF-1-beta
Short name:
HNF-1B
Alternative name(s):
Homeoprotein LFB3
Transcription factor 2
Short name:
TCF-2
Gene namesi
Name:Hnf1b
Synonyms:Hnf-1b, Tcf2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:98505. Hnf1b.

Subcellular locationi

GO - Cellular componenti

  1. nucleoplasm Source: Reactome
  2. nucleus Source: UniProtKB
  3. transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 558558Hepatocyte nuclear factor 1-betaPRO_0000049122Add
BLAST

Proteomic databases

MaxQBiP27889.
PaxDbiP27889.
PRIDEiP27889.

PTM databases

PhosphoSiteiP27889.

Expressioni

Gene expression databases

BgeeiP27889.
CleanExiMM_HNF1B.
ExpressionAtlasiP27889. baseline and differential.
GenevestigatoriP27889.

Interactioni

Subunit structurei

Binds DNA as a dimer. Can form homodimer or heterodimer with HNF1-alpha.

Protein-protein interaction databases

BioGridi204003. 5 interactions.
IntActiP27889. 1 interaction.
MINTiMINT-4097641.

Structurei

3D structure databases

ProteinModelPortaliP27889.
SMRiP27889. Positions 91-321.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 3131DimerizationBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the HNF1 homeobox family.Curated
Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG79356.
GeneTreeiENSGT00730000110937.
HOVERGENiHBG005980.
InParanoidiP27889.
KOiK08034.
OMAiMYTHKQE.
OrthoDBiEOG769ZJ9.
PhylomeDBiP27889.
TreeFamiTF320327.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR006899. HNF-1_N.
IPR023219. HNF1_dimer_dom.
IPR006897. HNF1b_C.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
[Graphical view]
PfamiPF04814. HNF-1_N. 1 hit.
PF04812. HNF-1B_C. 1 hit.
PF00046. Homeobox. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF100957. SSF100957. 1 hit.
SSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P27889-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVSKLTSLQQ ELLSALLSSG VTKEVLIQAL EELLPSPNFG VKLETLPLSP
60 70 80 90 100
GSGADLDTKP VFHTLTNGHA KGRLSGDEGS EDGDDYDTPP ILKELQALNT
110 120 130 140 150
EEAAEQRAEV DRMLSEDPWR AAKMIKGYMQ QHNIPQREVV DVTGLNQSHL
160 170 180 190 200
SQHLNKGTPM KTQKRAALYT WYVRKQREIL RQFNQTVQSS GNMTDKSSQD
210 220 230 240 250
QLLFLFPEFS QQNQGPGQSE DTCSEPTNKK MRRNRFKWGP ASQQILYQAY
260 270 280 290 300
DRQKNPSKEE REALVEECNR AECLQRGVSP SKAHGLGSNL VTEVRVYNWF
310 320 330 340 350
ANRRKEEAFR QKLAMDAYSS NQTHNLNPLL THGSPHHQPS SSPPNKMSGV
360 370 380 390 400
RYNQPGNNEV TSSSTISHHG NSAMVTSQSV LQQVSPASLD PGHSLLSPDS
410 420 430 440 450
KMQITVSGGG LPPVSTLTNI HSLSHHNPQQ SQNLIMTPLS GVMAIAQSLN
460 470 480 490 500
TSQAQGVPVI NSVASSLAAL QPVQFSQQLH SPHQQPLMQQ SPGSHMAQQP
510 520 530 540 550
FMAAVTQLQN SHMYAHKQEP PQYSHTSRFP SAMVVTDTSS INTLTSMSSS

KQCPLQAW
Length:558
Mass (Da):61,588
Last modified:April 4, 2003 - v2
Checksum:iD4F2BC92B64374A6
GO
Isoform 2 (identifier: P27889-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     183-208: Missing.

Show »
Length:532
Mass (Da):58,644
Checksum:iA7AD8DBD40D40E4F
GO
Isoform 3 (identifier: P27889-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-123: Missing.

Note: No experimental confirmation available.

Show »
Length:435
Mass (Da):48,336
Checksum:i8885C6A5474F7786
GO
Isoform 4 (identifier: P27889-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-123: Missing.
     183-208: Missing.

Note: No experimental confirmation available.

Show »
Length:409
Mass (Da):45,392
Checksum:i4B8FDE392F0D55CC
GO

Sequence cautioni

The sequence BAB31632.2 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti67 – 671N → S in BAB31632. (PubMed:16141072)Curated
Sequence conflicti423 – 4297LSHHNPQ → THSPPQSP in CAA39358. (PubMed:1673925)Curated
Sequence conflicti520 – 5212PP → HT in CAA39358. (PubMed:1673925)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti222 – 2221T → A in strain: NSY. 2 Publications

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 123123Missing in isoform 3 and isoform 4. 2 PublicationsVSP_007099Add
BLAST
Alternative sequencei183 – 20826Missing in isoform 2 and isoform 4. 2 PublicationsVSP_007100Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55842 mRNA. Translation: CAA39358.1.
AB008174 mRNA. Translation: BAA77718.1.
AB008175 mRNA. Translation: BAA77719.1.
AB008176 mRNA. Translation: BAA77720.1.
AB008177 mRNA. Translation: BAA77721.1.
AB052659 mRNA. Translation: BAB60814.1.
AK004837 mRNA. Translation: BAB23604.1.
AK019258 mRNA. Translation: BAB31632.2. Different initiation.
AL669868 Genomic DNA. Translation: CAI35313.1.
BC025189 mRNA. Translation: AAH25189.1.
CCDSiCCDS25179.1. [P27889-1]
CCDS70271.1. [P27889-2]
CCDS70272.1. [P27889-3]
PIRiA39633.
RefSeqiNP_001278197.1. NM_001291268.1. [P27889-2]
NP_001278198.1. NM_001291269.1. [P27889-3]
NP_033356.2. NM_009330.3. [P27889-1]
XP_006532859.1. XM_006532796.1. [P27889-3]
UniGeneiMm.7226.

Genome annotation databases

EnsembliENSMUST00000021016; ENSMUSP00000021016; ENSMUSG00000020679. [P27889-1]
ENSMUST00000108113; ENSMUSP00000103748; ENSMUSG00000020679. [P27889-3]
ENSMUST00000108114; ENSMUSP00000103749; ENSMUSG00000020679. [P27889-2]
GeneIDi21410.
KEGGimmu:21410.
UCSCiuc007kpx.1. mouse. [P27889-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55842 mRNA. Translation: CAA39358.1.
AB008174 mRNA. Translation: BAA77718.1.
AB008175 mRNA. Translation: BAA77719.1.
AB008176 mRNA. Translation: BAA77720.1.
AB008177 mRNA. Translation: BAA77721.1.
AB052659 mRNA. Translation: BAB60814.1.
AK004837 mRNA. Translation: BAB23604.1.
AK019258 mRNA. Translation: BAB31632.2. Different initiation.
AL669868 Genomic DNA. Translation: CAI35313.1.
BC025189 mRNA. Translation: AAH25189.1.
CCDSiCCDS25179.1. [P27889-1]
CCDS70271.1. [P27889-2]
CCDS70272.1. [P27889-3]
PIRiA39633.
RefSeqiNP_001278197.1. NM_001291268.1. [P27889-2]
NP_001278198.1. NM_001291269.1. [P27889-3]
NP_033356.2. NM_009330.3. [P27889-1]
XP_006532859.1. XM_006532796.1. [P27889-3]
UniGeneiMm.7226.

3D structure databases

ProteinModelPortaliP27889.
SMRiP27889. Positions 91-321.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204003. 5 interactions.
IntActiP27889. 1 interaction.
MINTiMINT-4097641.

PTM databases

PhosphoSiteiP27889.

Proteomic databases

MaxQBiP27889.
PaxDbiP27889.
PRIDEiP27889.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021016; ENSMUSP00000021016; ENSMUSG00000020679. [P27889-1]
ENSMUST00000108113; ENSMUSP00000103748; ENSMUSG00000020679. [P27889-3]
ENSMUST00000108114; ENSMUSP00000103749; ENSMUSG00000020679. [P27889-2]
GeneIDi21410.
KEGGimmu:21410.
UCSCiuc007kpx.1. mouse. [P27889-1]

Organism-specific databases

CTDi6928.
MGIiMGI:98505. Hnf1b.

Phylogenomic databases

eggNOGiNOG79356.
GeneTreeiENSGT00730000110937.
HOVERGENiHBG005980.
InParanoidiP27889.
KOiK08034.
OMAiMYTHKQE.
OrthoDBiEOG769ZJ9.
PhylomeDBiP27889.
TreeFamiTF320327.

Enzyme and pathway databases

ReactomeiREACT_208465. Regulation of gene expression in early pancreatic precursor cells.
REACT_222486. Regulation of gene expression in late stage (branching morphogenesis) pancreatic bud precursor cells.

Miscellaneous databases

NextBioi300688.
PROiP27889.
SOURCEiSearch...

Gene expression databases

BgeeiP27889.
CleanExiMM_HNF1B.
ExpressionAtlasiP27889. baseline and differential.
GenevestigatoriP27889.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR006899. HNF-1_N.
IPR023219. HNF1_dimer_dom.
IPR006897. HNF1b_C.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
[Graphical view]
PfamiPF04814. HNF-1_N. 1 hit.
PF04812. HNF-1B_C. 1 hit.
PF00046. Homeobox. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF100957. SSF100957. 1 hit.
SSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "LFB3, a heterodimer-forming homeoprotein of the LFB1 family, is expressed in specialized epithelia."
    de Simone V., de Magistris L., Lazzaro L., Gerstner J., Monaci P., Nicosia A., Cortese R.
    EMBO J. 10:1435-1443(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "Genetic analysis of late-onset type 2 diabetes in a mouse model of human complex trait."
    Ueda H., Ikegami H., Kawaguchi Y., Fujisawa T., Yamato E., Shibata M., Ogihara T.
    Diabetes 48:1168-1174(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), VARIANT ALA-222.
    Strain: C3H and NSY.
    Tissue: Kidney.
  3. "Sequence analysis of candidate genes for common susceptibility to type 1 and type 2 diabetes in mice."
    Yamada K., Ikegami H., Kawaguchi Y., Fujisawa T., Hotta M., Ueda H., Shintani M., Nojima K., Kawabata Y., Ono M., Nishino M., Itoi M., Babaya N., Shibata M., Makino S., Ogihara T.
    Endocr. J. 48:241-247(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT ALA-222.
    Strain: C3H, NOD and NSY.
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 4).
    Strain: C57BL/6J.
    Tissue: Embryo and Liver.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Tissue: Kidney.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiHNF1B_MOUSE
AccessioniPrimary (citable) accession number: P27889
Secondary accession number(s): Q5NC37
, Q8R162, Q9CS26, Q9R1W1, Q9R1W2, Q9WTL5, Q9WTL6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: April 4, 2003
Last modified: February 4, 2015
This is version 144 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.