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Protein

Cytosol aminopeptidase

Gene

pepA

Organism
Rickettsia prowazekii (strain Madrid E)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides.

Catalytic activityi

Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi265 – 2651Manganese 2By similarity
Metal bindingi270 – 2701Manganese 1By similarity
Metal bindingi270 – 2701Manganese 2By similarity
Active sitei277 – 2771Sequence Analysis
Metal bindingi288 – 2881Manganese 2By similarity
Metal bindingi347 – 3471Manganese 1By similarity
Metal bindingi349 – 3491Manganese 1By similarity
Metal bindingi349 – 3491Manganese 2By similarity
Active sitei351 – 3511Sequence Analysis

GO - Molecular functioni

  1. aminopeptidase activity Source: UniProtKB-HAMAP
  2. manganese ion binding Source: UniProtKB-HAMAP
  3. metalloexopeptidase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Protease

Keywords - Ligandi

Manganese, Metal-binding

Protein family/group databases

MEROPSiM17.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosol aminopeptidase (EC:3.4.11.1)
Alternative name(s):
Leucine aminopeptidase (EC:3.4.11.10)
Short name:
LAP
Leucyl aminopeptidase
Gene namesi
Name:pepA
Ordered Locus Names:RP142
OrganismiRickettsia prowazekii (strain Madrid E)
Taxonomic identifieri272947 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group
ProteomesiUP000002480 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 500500Cytosol aminopeptidasePRO_0000165792Add
BLAST

Proteomic databases

PRIDEiP27888.

Interactioni

Protein-protein interaction databases

STRINGi272947.RP142.

Structurei

3D structure databases

ProteinModelPortaliP27888.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M17 family.Curated

Phylogenomic databases

eggNOGiCOG0260.
HOGENOMiHOG000243129.
KOiK01255.
OMAiNTPPMIG.
OrthoDBiEOG6FV8B3.

Family and domain databases

HAMAPiMF_00181. Cytosol_peptidase_M17.
InterProiIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
IPR023042. Peptidase_M17_leu_NH2_pept.
IPR008283. Peptidase_M17_N.
[Graphical view]
PfamiPF00883. Peptidase_M17. 1 hit.
PF02789. Peptidase_M17_N. 1 hit.
[Graphical view]
PRINTSiPR00481. LAMNOPPTDASE.
PROSITEiPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P27888-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLNINFVNEE SSTNQGLIVF IDEQLKLNNN LIALDQQHYE LISKTIQNKL
60 70 80 90 100
QFSGNYGQIT VVPSVIKSCA VKYLIIVGLG NVEKLTEAKI EELGGKILQH
110 120 130 140 150
ATCAKIATIG LKIINRINRF TSPTFTSLIA SGAFLASYRF HKYKTTLKEV
160 170 180 190 200
EKFAVESIEI LTDNNSEAMK LFEVKKLIAE AVFFTRDISN EPSNIKTPQV
210 220 230 240 250
YAERIVEILE PLGVNIDVIG EHDIKNLGMG ALLGVGQGSQ NESKLVVMEY
260 270 280 290 300
KGGSRDDSTL ALVGKGVIFD TGGISLKPSS NMHLMRYDMA GSAAVVGTII
310 320 330 340 350
ALASQKVPVN VVGVVGLVEN MQSGNAQRPG DVVVTMSGQT AEVLNTDAEG
360 370 380 390 400
RLVLADTVWY VQEKFNPKCV IDVATLTGAI TVALGSTYAG CFSNNDELAD
410 420 430 440 450
KLIKAGEAVN EKLWRMPLHD DYDAMINSDI ADIANIGNVP GAAGSCTAAH
460 470 480 490 500
FIKRFIKDGV DWAHLDIAGV ANSNNASALC PKGAVGYGVR LLEKFIKEYN
Length:500
Mass (Da):54,006
Last modified:August 1, 1992 - v1
Checksum:iE87FFDEA47EC7C83
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M68966 Genomic DNA. Translation: AAA26388.1.
AJ235270 Genomic DNA. Translation: CAA14610.1.
PIRiA40631.
RefSeqiNP_220533.1. NC_000963.1.
WP_004597194.1. NC_000963.1.

Genome annotation databases

GeneIDi883797.
KEGGirpr:RP142.
PATRICi17901028. VBIRicPro72556_0145.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M68966 Genomic DNA. Translation: AAA26388.1.
AJ235270 Genomic DNA. Translation: CAA14610.1.
PIRiA40631.
RefSeqiNP_220533.1. NC_000963.1.
WP_004597194.1. NC_000963.1.

3D structure databases

ProteinModelPortaliP27888.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272947.RP142.

Protein family/group databases

MEROPSiM17.003.

Proteomic databases

PRIDEiP27888.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi883797.
KEGGirpr:RP142.
PATRICi17901028. VBIRicPro72556_0145.

Phylogenomic databases

eggNOGiCOG0260.
HOGENOMiHOG000243129.
KOiK01255.
OMAiNTPPMIG.
OrthoDBiEOG6FV8B3.

Family and domain databases

HAMAPiMF_00181. Cytosol_peptidase_M17.
InterProiIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
IPR023042. Peptidase_M17_leu_NH2_pept.
IPR008283. Peptidase_M17_N.
[Graphical view]
PfamiPF00883. Peptidase_M17. 1 hit.
PF02789. Peptidase_M17_N. 1 hit.
[Graphical view]
PRINTSiPR00481. LAMNOPPTDASE.
PROSITEiPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of the Rickettsia prowazekii pepA gene encoding leucine aminopeptidase."
    Wood D.O., Solomon M.J., Speed R.R.
    J. Bacteriol. 175:159-165(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Madrid E.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Madrid E.

Entry informationi

Entry nameiAMPA_RICPR
AccessioniPrimary (citable) accession number: P27888
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: April 1, 2015
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. Rickettsia prowazekii
    Rickettsia prowazekii (strain Madrid E): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.