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P27888

- AMPA_RICPR

UniProt

P27888 - AMPA_RICPR

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Protein
Cytosol aminopeptidase
Gene
pepA, RP142
Organism
Rickettsia prowazekii (strain Madrid E)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides.UniRule annotation

Catalytic activityi

Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.UniRule annotation
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.

Cofactori

Binds 2 manganese ions per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi265 – 2651Manganese 2 By similarity
Metal bindingi270 – 2701Manganese 1 By similarity
Metal bindingi270 – 2701Manganese 2 By similarity
Active sitei277 – 2771 Reviewed prediction
Metal bindingi288 – 2881Manganese 2 By similarity
Metal bindingi347 – 3471Manganese 1 By similarity
Metal bindingi349 – 3491Manganese 1 By similarity
Metal bindingi349 – 3491Manganese 2 By similarity
Active sitei351 – 3511 Reviewed prediction

GO - Molecular functioni

  1. aminopeptidase activity Source: UniProtKB-HAMAP
  2. manganese ion binding Source: UniProtKB-HAMAP
  3. metalloexopeptidase activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Aminopeptidase, Hydrolase, Protease

    Keywords - Ligandi

    Manganese, Metal-binding

    Protein family/group databases

    MEROPSiM17.003.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytosol aminopeptidase (EC:3.4.11.1)
    Alternative name(s):
    Leucine aminopeptidase (EC:3.4.11.10)
    Short name:
    LAP
    Leucyl aminopeptidase
    Gene namesi
    Name:pepA
    Ordered Locus Names:RP142
    OrganismiRickettsia prowazekii (strain Madrid E)
    Taxonomic identifieri272947 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group
    ProteomesiUP000002480: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 500500Cytosol aminopeptidaseUniRule annotation
    PRO_0000165792Add
    BLAST

    Proteomic databases

    PRIDEiP27888.

    Interactioni

    Protein-protein interaction databases

    STRINGi272947.RP142.

    Structurei

    3D structure databases

    ProteinModelPortaliP27888.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase M17 family.

    Phylogenomic databases

    eggNOGiCOG0260.
    HOGENOMiHOG000243129.
    KOiK01255.
    OMAiDSQFADM.
    OrthoDBiEOG6FV8B3.

    Family and domain databases

    HAMAPiMF_00181. Cytosol_peptidase_M17.
    InterProiIPR011356. Leucine_aapep/pepB.
    IPR000819. Peptidase_M17_C.
    IPR023042. Peptidase_M17_leu_NH2_pept.
    IPR008283. Peptidase_M17_N.
    [Graphical view]
    PfamiPF00883. Peptidase_M17. 1 hit.
    PF02789. Peptidase_M17_N. 1 hit.
    [Graphical view]
    PRINTSiPR00481. LAMNOPPTDASE.
    PROSITEiPS00631. CYTOSOL_AP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P27888-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLNINFVNEE SSTNQGLIVF IDEQLKLNNN LIALDQQHYE LISKTIQNKL    50
    QFSGNYGQIT VVPSVIKSCA VKYLIIVGLG NVEKLTEAKI EELGGKILQH 100
    ATCAKIATIG LKIINRINRF TSPTFTSLIA SGAFLASYRF HKYKTTLKEV 150
    EKFAVESIEI LTDNNSEAMK LFEVKKLIAE AVFFTRDISN EPSNIKTPQV 200
    YAERIVEILE PLGVNIDVIG EHDIKNLGMG ALLGVGQGSQ NESKLVVMEY 250
    KGGSRDDSTL ALVGKGVIFD TGGISLKPSS NMHLMRYDMA GSAAVVGTII 300
    ALASQKVPVN VVGVVGLVEN MQSGNAQRPG DVVVTMSGQT AEVLNTDAEG 350
    RLVLADTVWY VQEKFNPKCV IDVATLTGAI TVALGSTYAG CFSNNDELAD 400
    KLIKAGEAVN EKLWRMPLHD DYDAMINSDI ADIANIGNVP GAAGSCTAAH 450
    FIKRFIKDGV DWAHLDIAGV ANSNNASALC PKGAVGYGVR LLEKFIKEYN 500
    Length:500
    Mass (Da):54,006
    Last modified:August 1, 1992 - v1
    Checksum:iE87FFDEA47EC7C83
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M68966 Genomic DNA. Translation: AAA26388.1.
    AJ235270 Genomic DNA. Translation: CAA14610.1.
    PIRiA40631.
    RefSeqiNP_220533.1. NC_000963.1.
    WP_004597194.1. NC_000963.1.

    Genome annotation databases

    EnsemblBacteriaiCAA14610; CAA14610; CAA14610.
    GeneIDi883797.
    KEGGirpr:RP142.
    PATRICi17901028. VBIRicPro72556_0145.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M68966 Genomic DNA. Translation: AAA26388.1 .
    AJ235270 Genomic DNA. Translation: CAA14610.1 .
    PIRi A40631.
    RefSeqi NP_220533.1. NC_000963.1.
    WP_004597194.1. NC_000963.1.

    3D structure databases

    ProteinModelPortali P27888.
    ModBasei Search...

    Protein-protein interaction databases

    STRINGi 272947.RP142.

    Protein family/group databases

    MEROPSi M17.003.

    Proteomic databases

    PRIDEi P27888.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAA14610 ; CAA14610 ; CAA14610 .
    GeneIDi 883797.
    KEGGi rpr:RP142.
    PATRICi 17901028. VBIRicPro72556_0145.

    Phylogenomic databases

    eggNOGi COG0260.
    HOGENOMi HOG000243129.
    KOi K01255.
    OMAi DSQFADM.
    OrthoDBi EOG6FV8B3.

    Family and domain databases

    HAMAPi MF_00181. Cytosol_peptidase_M17.
    InterProi IPR011356. Leucine_aapep/pepB.
    IPR000819. Peptidase_M17_C.
    IPR023042. Peptidase_M17_leu_NH2_pept.
    IPR008283. Peptidase_M17_N.
    [Graphical view ]
    Pfami PF00883. Peptidase_M17. 1 hit.
    PF02789. Peptidase_M17_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00481. LAMNOPPTDASE.
    PROSITEi PS00631. CYTOSOL_AP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    « Hide 'large scale' publications
    1. "Characterization of the Rickettsia prowazekii pepA gene encoding leucine aminopeptidase."
      Wood D.O., Solomon M.J., Speed R.R.
      J. Bacteriol. 175:159-165(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Madrid E.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Madrid E.

    Entry informationi

    Entry nameiAMPA_RICPR
    AccessioniPrimary (citable) accession number: P27888
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: August 1, 1992
    Last modified: September 3, 2014
    This is version 99 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. Rickettsia prowazekii
      Rickettsia prowazekii (strain Madrid E): entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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