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Protein

Cytosol aminopeptidase

Gene

pepA

Organism
Rickettsia prowazekii (strain Madrid E)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides.

Catalytic activityi

Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi265Manganese 2By similarity1
Metal bindingi270Manganese 1By similarity1
Metal bindingi270Manganese 2By similarity1
Active sitei277Sequence analysis1
Metal bindingi288Manganese 2By similarity1
Metal bindingi347Manganese 1By similarity1
Metal bindingi349Manganese 1By similarity1
Metal bindingi349Manganese 2By similarity1
Active sitei351Sequence analysis1

GO - Molecular functioni

Keywordsi

Molecular functionAminopeptidase, Hydrolase, Protease
LigandManganese, Metal-binding

Enzyme and pathway databases

BioCyciRPRO272947:G1GT0-144-MONOMER

Protein family/group databases

MEROPSiM17.003

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosol aminopeptidase (EC:3.4.11.1)
Alternative name(s):
Leucine aminopeptidase (EC:3.4.11.10)
Short name:
LAP
Leucyl aminopeptidase
Gene namesi
Name:pepA
Ordered Locus Names:RP142
OrganismiRickettsia prowazekii (strain Madrid E)
Taxonomic identifieri272947 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group
Proteomesi
  • UP000002480 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001657921 – 500Cytosol aminopeptidaseAdd BLAST500

Interactioni

Protein-protein interaction databases

STRINGi272947.RP142

Structurei

3D structure databases

ProteinModelPortaliP27888
SMRiP27888
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M17 family.Curated

Phylogenomic databases

eggNOGiENOG4105BZ6 Bacteria
COG0260 LUCA
HOGENOMiHOG000243129
KOiK01255
OMAiMKNTGPR

Family and domain databases

CDDicd00433 Peptidase_M17, 1 hit
HAMAPiMF_00181 Cytosol_peptidase_M17, 1 hit
InterProiView protein in InterPro
IPR011356 Leucine_aapep/pepB
IPR000819 Peptidase_M17_C
IPR023042 Peptidase_M17_leu_NH2_pept
IPR008283 Peptidase_M17_N
PANTHERiPTHR11963 PTHR11963, 1 hit
PfamiView protein in Pfam
PF00883 Peptidase_M17, 1 hit
PF02789 Peptidase_M17_N, 1 hit
PRINTSiPR00481 LAMNOPPTDASE
PROSITEiView protein in PROSITE
PS00631 CYTOSOL_AP, 1 hit

Sequencei

Sequence statusi: Complete.

P27888-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLNINFVNEE SSTNQGLIVF IDEQLKLNNN LIALDQQHYE LISKTIQNKL
60 70 80 90 100
QFSGNYGQIT VVPSVIKSCA VKYLIIVGLG NVEKLTEAKI EELGGKILQH
110 120 130 140 150
ATCAKIATIG LKIINRINRF TSPTFTSLIA SGAFLASYRF HKYKTTLKEV
160 170 180 190 200
EKFAVESIEI LTDNNSEAMK LFEVKKLIAE AVFFTRDISN EPSNIKTPQV
210 220 230 240 250
YAERIVEILE PLGVNIDVIG EHDIKNLGMG ALLGVGQGSQ NESKLVVMEY
260 270 280 290 300
KGGSRDDSTL ALVGKGVIFD TGGISLKPSS NMHLMRYDMA GSAAVVGTII
310 320 330 340 350
ALASQKVPVN VVGVVGLVEN MQSGNAQRPG DVVVTMSGQT AEVLNTDAEG
360 370 380 390 400
RLVLADTVWY VQEKFNPKCV IDVATLTGAI TVALGSTYAG CFSNNDELAD
410 420 430 440 450
KLIKAGEAVN EKLWRMPLHD DYDAMINSDI ADIANIGNVP GAAGSCTAAH
460 470 480 490 500
FIKRFIKDGV DWAHLDIAGV ANSNNASALC PKGAVGYGVR LLEKFIKEYN
Length:500
Mass (Da):54,006
Last modified:August 1, 1992 - v1
Checksum:iE87FFDEA47EC7C83
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M68966 Genomic DNA Translation: AAA26388.1
AJ235270 Genomic DNA Translation: CAA14610.1
PIRiA40631
RefSeqiNP_220533.1, NC_000963.1
WP_004597194.1, NC_000963.1

Genome annotation databases

EnsemblBacteriaiCAA14610; CAA14610; CAA14610
GeneIDi883797
KEGGirpr:RP142
PATRICifig|272947.5.peg.145

Similar proteinsi

Entry informationi

Entry nameiAMPA_RICPR
AccessioniPrimary (citable) accession number: P27888
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: May 23, 2018
This is version 114 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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