P27888 (AMPA_RICPR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytosol aminopeptidase
EC=3.4.11.1

Alternative name(s):
Leucine aminopeptidase
Short name=LAP
EC=3.4.11.10
Leucyl aminopeptidase

Gene names

Name:	pepA
Ordered Locus Names:	RP142

Organism

Rickettsia prowazekii (strain Madrid E)

Taxonomic identifier

272947 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	500 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides. HAMAP MF_00181
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP MF_00181 Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.
Cofactor	Binds 2 manganese ions per subunit By similarity. HAMAP MF_00181
Subcellular location	Cytoplasm HAMAP MF_00181.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: InterPro metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 500	500	Cytosol aminopeptidase HAMAP MF_00181		PRO_0000165792
Sites
	Active site	277	1	Potential
	Active site	351	1	Potential
	Metal binding	265	1	Manganese 2 By similarity
	Metal binding	270	1	Manganese 1 By similarity
	Metal binding	270	1	Manganese 2 By similarity
	Metal binding	288	1	Manganese 2 By similarity
	Metal binding	347	1	Manganese 1 By similarity
	Metal binding	349	1	Manganese 1 By similarity
	Metal binding	349	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P27888 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: E87FFDEA47EC7C83	FASTA	500	54,006
	10 20 30 40 50 60 MLNINFVNEE SSTNQGLIVF IDEQLKLNNN LIALDQQHYE LISKTIQNKL QFSGNYGQIT 70 80 90 100 110 120 VVPSVIKSCA VKYLIIVGLG NVEKLTEAKI EELGGKILQH ATCAKIATIG LKIINRINRF 130 140 150 160 170 180 TSPTFTSLIA SGAFLASYRF HKYKTTLKEV EKFAVESIEI LTDNNSEAMK LFEVKKLIAE 190 200 210 220 230 240 AVFFTRDISN EPSNIKTPQV YAERIVEILE PLGVNIDVIG EHDIKNLGMG ALLGVGQGSQ 250 260 270 280 290 300 NESKLVVMEY KGGSRDDSTL ALVGKGVIFD TGGISLKPSS NMHLMRYDMA GSAAVVGTII 310 320 330 340 350 360 ALASQKVPVN VVGVVGLVEN MQSGNAQRPG DVVVTMSGQT AEVLNTDAEG RLVLADTVWY 370 380 390 400 410 420 VQEKFNPKCV IDVATLTGAI TVALGSTYAG CFSNNDELAD KLIKAGEAVN EKLWRMPLHD 430 440 450 460 470 480 DYDAMINSDI ADIANIGNVP GAAGSCTAAH FIKRFIKDGV DWAHLDIAGV ANSNNASALC 490 500 PKGAVGYGVR LLEKFIKEYN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the Rickettsia prowazekii pepA gene encoding leucine aminopeptidase." Wood D.O., Solomon M.J., Speed R.R. J. Bacteriol. 175:159-165(1993) [PubMed: 8416891] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Madrid E.
[2]	"The genome sequence of Rickettsia prowazekii and the origin of mitochondria." Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G. Nature 396:133-140(1998) [PubMed: 9823893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Madrid E.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M68966 Genomic DNA. Translation: AAA26388.1. AJ235270 Genomic DNA. Translation: CAA14610.1.
PIR	A40631.
RefSeq	NP_220533.1. NC_000963.1.
3D structure databases
ProteinModelPortal	P27888.
ModBase	Search...
Protein family/group databases
MEROPS	M17.003.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	883797.
GenomeReviews	Gene locus RP142 in contig AJ235269_GR.
KEGG	rpr:RP142.
PATRIC	17901028. VBIRicPro72556_0145.
Phylogenomic databases
HOGENOM	HBG742580.
OMA	NMHLMRY.
ProtClustDB	PRK00913.
Enzyme and pathway databases
BioCyc	RPRO272947:RP142-MONOMER.
Family and domain databases
HAMAP	MF_00181. Cytosol_peptidase_M17. [Tree]
InterPro	IPR011356. Peptidase_M17. IPR000819. Peptidase_M17_C. IPR023042. Peptidase_M17_cytosol_amino. IPR008283. Peptidase_M17_N. [Graphical view]
KO	K01255.
PANTHER	PTHR11963:SF3. Peptidase_M17. 1 hit.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPA_RICPR

Accession

Primary (citable) accession number: P27888

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	January 25, 2012
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program