Reviewed, UniProtKB/Swiss-Prot P27887 (XYLE_PSEAE)

Names and origin

Protein names

Recommended name:
    Metapyrocatechase
      Short name=MPC
    EC=1.13.11.2
Alternative name(s):
    CatO2ase
    Catechol 2,3-dioxygenase

Gene names

Name:

bztE

Organism

Pseudomonas aeruginosa

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	307 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Catechol + O2 = 2-hydroxymuconate semialdehyde.
Cofactor	Fe2+ ion.
Pathway	Xenobiotic degradation; toluene degradation.
Subunit structure	Homotetramer.
Sequence similarities	Belongs to the extradiol ring-cleavage dioxygenase family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catechol 2,3-dioxygenase activity Inferred from electronic annotation. Source: EC ferrous iron binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 307	307	Metapyrocatechase		PRO_0000085026
Sites
	Metal binding	153	1	Iron By similarity
	Metal binding	214	1	Iron By similarity
	Metal binding	265	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	P27887-1 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: E178E5F287410ACC	FASTA	307	35,124
	10 20 30 40 50 60 MNKGIMRPGH VQLRVLDMSK ALEHYVELLG LIEMDRDDQG RVYLKAWTEV DKFSVVLRER 70 80 90 100 110 120 DEPGMDFMGF KVVDEDALRQ LERDLTAYGC AVEQLPAGEL NSCGRRVRFQ APSGHHFELY 130 140 150 160 170 180 ADKEYTGKWG VNEVNPEAWP RDLKGMAAVR FDHCLLYGDE LPATYDLFTK VLGFYLAEQV 190 200 210 220 230 240 LDENGTRVAQ FLSLSTKAHD VAFIHHPEKG RLHHVSFHLE TWEDVLRAAD LISMTDTSID 250 260 270 280 290 300 IGPTRHGLTH GKTIYFFDPS GNRSEVFCGG NYSYPDHKPV TWLAKDLGKA IFYHDRVLNE RFMTVLT

References

[1]

"Cloning and characterization of extradiol aromatic ring-cleavage dioxygenases of Pseudomonas aeruginosa JI104." Kitayama A., Achioku T., Yanagawa T., Kanou K., Kikuchi M., Ueda H., Suzuki E., Nishimura H., Nagamune T., Kawakami Y. J. Ferment. Bioeng. 82:217-223(1996) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: JI104.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X60740 Genomic DNA. Translation: CAA43145.1.
PIR	JC2503. S15522.
3D structure databases
HSSP	HSSP built from PDB template 1MPY based on UniProtKB P06622.
SMR	P27887. Positions 1-307.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.13.11.2. 354.
Family and domain databases
InterPro	IPR017624. Catechol_2-3_dOase. IPR004360. Glyas_bleo-R_dOase. IPR000486. Xdiol_dOase_1_2. [Graphical view]
Pfam	PF00903. Glyoxalase. 2 hits. [Graphical view]
ProDom	PD002334. Gly_diox. 2 hits. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR03211. catechol_2_3. 1 hit.
PROSITE	PS00082. EXTRADIOL_DIOXYGENAS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XYLE_PSEAE

Accession

Primary (citable) accession number: P27887

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	June 16, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)