P27882 (ERV1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitochondrial FAD-linked sulfhydryl oxidase ERV1
EC=1.8.3.2

Alternative name(s):
14 kDa regulatory protein
Essential for respiration and vegetative growth protein 1

Gene names

Name:	ERV1
Ordered Locus Names:	YGR029W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	189 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	FAD-dependent sulfhydryl oxidase that catalyzes disulfide bond formation. Required for the import and folding of small cysteine-containing proteins in the mitochondrial intermembrane space (IMS). Forms a redox cycle with MIA40 that involves a disulfide relay system. Important for maintaining the cysteine residues in MIA40 in an oxidized state. Reduced ERV1 is reoxidized by cytochrome c. Required for the maturation of cytoplasmic, but not of mitochondrial Fe/S proteins. Ref.7 Ref.8 Ref.9 Ref.11 Ref.12 Ref.13
Catalytic activity	2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2.
Cofactor	FAD.
Subunit structure	Homodimer. Interacts with MIA40, forming transient intermolecular disulfide bridges. Ref.8 Ref.11 Ref.12
Subcellular location	Mitochondrion intermembrane space Ref.9 Ref.10.
Sequence similarities	Contains 1 ERV/ALR sulfhydryl oxidase domain.
Sequence caution	The sequence AAB48659.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAA43129.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAA48192.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Mitochondrion
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular iron ion homeostasis Inferred from direct assay Ref.9. Source: SGD cellular response to oxidative stress Inferred from mutant phenotype Ref.11. Source: SGD protein import into mitochondrial intermembrane space Inferred from mutant phenotype Ref.11Ref.12. Source: SGD
Cellular component	mitochondrial intermembrane space Inferred from direct assay Ref.9. Source: SGD
Molecular function	flavin-linked sulfhydryl oxidase activity Inferred from direct assay Ref.7. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 189	189	Mitochondrial FAD-linked sulfhydryl oxidase ERV1		PRO_0000001187
Regions
	Domain	83 – 183	101	ERV/ALR sulfhydryl oxidase
	Region	166 – 183	18	FAD-binding By similarity
Amino acid modifications
	Disulfide bond	130 ↔ 133		Redox-active By similarity
	Disulfide bond	159 ↔ 176		By similarity
Experimental info
	Mutagenesis	30	1	C → S: Reduces catalytic activity 3-fold. Ref.8
	Mutagenesis	33	1	C → S: Reduces catalytic activity 2-fold. Ref.8
	Mutagenesis	130	1	C → S: Loss of function. Ref.8
	Mutagenesis	133	1	C → S: Loss of function. Ref.8
	Mutagenesis	159	1	C → S: Reduces catalytic activity 3.3-fold. Ref.8
	Mutagenesis	176	1	C → S: Reduces catalytic activity 2.6-fold. Ref.8

Sequences

	Sequence		Length	Mass (Da)
	P27882 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 83DADA0DB1DF17CC	FASTA	189	21,639
	10 20 30 40 50 60 MKAIDKMTDN PPQEGLSGRK IIYDEDGKPC RSCNTLLDFQ YVTGKISNGL KNLSSNGKLA 70 80 90 100 110 120 GTGALTGEAS ELMPGSRTYR KVDPPDVEQL GRSSWTLLHS VAASYPAQPT DQQKGEMKQF 130 140 150 160 170 180 LNIFSHIYPC NWCAKDFEKY IRENAPQVES REELGRWMCE AHNKVNKKLR KPKFDCNFWE KRWKDGWDE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Dual function of a new nuclear gene for oxidative phosphorylation and vegetative growth in yeast." Lisowsky T. Mol. Gen. Genet. 232:58-64(1992) [PubMed: 1552903] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SC167.
[2]	"Intramitochondrial protein sorting. Isolation and characterization of the yeast MSP1 gene which belongs to a novel family of putative ATPases." Nakai M., Endo T., Hase T., Matsubara H. J. Biol. Chem. 268:24262-24269(1993) [PubMed: 8226973] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SF747-19D.
[3]	"Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII." Rieger M., Brueckner M., Schaefer M., Mueller-Auer S. Yeast 13:1077-1090(1997) [PubMed: 9290212] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[6]	"Removal of an intron with unique 3' branch site creates an amino-terminal protein sequence directing the scERV1 gene product to mitochondria." Lisowsky T. Yeast 12:1501-1510(1996) [PubMed: 8972573] [Abstract] Cited for: REVISION OF GENE MODEL.
[7]	"Erv1p from Saccharomyces cerevisiae is a FAD-linked sulfhydryl oxidase." Lee J.-E., Hofhaus G., Lisowsky T. FEBS Lett. 477:62-66(2000) [PubMed: 10899311] [Abstract] Cited for: FUNCTION, FAD-BINDING.
[8]	"The N-terminal cysteine pair of yeast sulfhydryl oxidase Erv1p is essential for in vivo activity and interacts with the primary redox centre." Hofhaus G., Lee J.E., Tews I., Rosenberg B., Lisowsky T. Eur. J. Biochem. 270:1528-1535(2003) [PubMed: 12654008] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF CYS-30; CYS-33; CYS-130; CYS-133; CYS-159 AND CYS-176, SUBUNIT.
[9]	"An essential function of the mitochondrial sulfhydryl oxidase Erv1p/ALR in the maturation of cytosolic Fe/S proteins." Lange H., Lisowsky T., Gerber J., Muhlenhoff U., Kispal G., Lill R. EMBO Rep. 2:715-720(2001) [PubMed: 11493598] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[10]	"The proteome of Saccharomyces cerevisiae mitochondria." Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C. Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed: 14576278] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Strain: ATCC 76625 / YPH499.
[11]	"A disulfide relay system in the intermembrane space of mitochondria that mediates protein import." Mesecke N., Terziyska N., Kozany C., Baumann F., Neupert W., Hell K., Herrmann J.M. Cell 121:1059-1069(2005) [PubMed: 15989955] [Abstract] Cited for: FUNCTION, INTERACTION WITH MIA40.
[12]	"The essential mitochondrial protein Erv1 cooperates with Mia40 in biogenesis of intermembrane space proteins." Rissler M., Wiedemann N., Pfannschmidt S., Gabriel K., Guiard B., Pfanner N., Chacinska A. J. Mol. Biol. 353:485-492(2005) [PubMed: 16181637] [Abstract] Cited for: FUNCTION, INTERACTION WITH MIA40.
[13]	"Erv1 mediates the Mia40-dependent protein import pathway and provides a functional link to the respiratory chain by shuttling electrons to cytochrome c." Allen S., Balabanidou V., Sideris D.P., Lisowsky T., Tokatlidis K. J. Mol. Biol. 353:937-944(2005) [PubMed: 16185707] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X60722 Genomic DNA. Translation: CAA43129.1. Sequence problems. M74772 Genomic DNA. Translation: AAB48659.1. Sequence problems. X68055 Genomic DNA. Translation: CAA48192.1. Sequence problems. Z72814 Genomic DNA. Translation: CAA97017.1. Z72813 Genomic DNA. Translation: CAA97016.1. BK006941 Genomic DNA. Translation: DAA08125.1.
PIR	S20469.
RefSeq	NP_011543.2. NM_001181158.1.
3D structure databases
ProteinModelPortal	P27882.
SMR	P27882. Positions 79-189.
ModBase	Search...
Protein-protein interaction databases
IntAct	P27882. 2 interactions.
STRING	P27882.
Proteomic databases
PeptideAtlas	P27882.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGR029W; YGR029W; YGR029W.
GeneID	852916.
KEGG	sce:YGR029W.
NMPDR	fig|4932.3.peg.2655.
Organism-specific databases
CYGD	YGR029w.
SGD	S000003261. ERV1.
Phylogenomic databases
eggNOG	fuNOG09123.
GeneTree	EFGT00050000003949.
HOGENOM	HBG315946.
OMA	DGKPCRS.
OrthoDB	EOG4CC79M.
Gene expression databases
ArrayExpress	P27882.
Genevestigator	P27882.
GermOnline	YGR029W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR017905. ERV/ALR_sulphydryl_oxidase. IPR006863. Evr1_Alr. [Graphical view]
Gene3D	G3DSA:1.20.120.310. Evr1_Alr. 1 hit.
Pfam	PF04777. Evr1_Alr. 1 hit. [Graphical view]
SUPFAM	SSF69000. Evr1_Alr. 1 hit.
PROSITE	PS51324. ERV_ALR. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	972617.

Entry information

Entry name

ERV1_YEAST

Accession

Primary (citable) accession number: P27882
Secondary accession number(s): D6VUG4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	November 1, 1997
Last modified:	December 14, 2011
This is version 103 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program