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P27882

- ERV1_YEAST

UniProt

P27882 - ERV1_YEAST

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Protein
Mitochondrial FAD-linked sulfhydryl oxidase ERV1
Gene
ERV1, YGR029W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

FAD-dependent sulfhydryl oxidase that catalyzes disulfide bond formation. Required for the import and folding of small cysteine-containing proteins in the mitochondrial intermembrane space (IMS). Forms a redox cycle with MIA40 that involves a disulfide relay system. Important for maintaining the cysteine residues in MIA40 in an oxidized state. Reduced ERV1 is reoxidized by cytochrome c. Required for the maturation of cytoplasmic, but not of mitochondrial Fe/S proteins.6 Publications

Catalytic activityi

2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2.

Cofactori

FAD.

GO - Molecular functioni

  1. flavin-linked sulfhydryl oxidase activity Source: SGD
  2. protein binding Source: IntAct
  3. thiol oxidase activity Source: SGD

GO - Biological processi

  1. cellular iron ion homeostasis Source: SGD
  2. cellular response to oxidative stress Source: SGD
  3. protein import into mitochondrial intermembrane space Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciYEAST:G3O-30753-MONOMER.
ReactomeiREACT_189012. Mitochondrial protein import.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial FAD-linked sulfhydryl oxidase ERV1 (EC:1.8.3.2)
Alternative name(s):
14 kDa regulatory protein
Essential for respiration and vegetative growth protein 1
Gene namesi
Name:ERV1
Ordered Locus Names:YGR029W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGR029w.
SGDiS000003261. ERV1.

Subcellular locationi

Mitochondrion intermembrane space 2 Publications

GO - Cellular componenti

  1. mitochondrial intermembrane space Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi30 – 301C → S: Reduces catalytic activity 3-fold. 1 Publication
Mutagenesisi33 – 331C → S: Reduces catalytic activity 2-fold. 1 Publication
Mutagenesisi130 – 1301C → S: Loss of function. 1 Publication
Mutagenesisi133 – 1331C → S: Loss of function. 1 Publication
Mutagenesisi159 – 1591C → S: Reduces catalytic activity 3.3-fold. 1 Publication
Mutagenesisi176 – 1761C → S: Reduces catalytic activity 2.6-fold. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 189189Mitochondrial FAD-linked sulfhydryl oxidase ERV1
PRO_0000001187Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi130 ↔ 133Redox-active By similarity
Disulfide bondi159 ↔ 176 By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiP27882.
PaxDbiP27882.
PeptideAtlasiP27882.

Expressioni

Gene expression databases

GenevestigatoriP27882.

Interactioni

Subunit structurei

Homodimer. Interacts with MIA40, forming transient intermolecular disulfide bridges.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MIA40P360462EBI-6621,EBI-26978

Protein-protein interaction databases

BioGridi33273. 22 interactions.
IntActiP27882. 5 interactions.
STRINGi4932.YGR029W.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi16 – 183
Beta strandi26 – 294
Helixi36 – 438
Turni44 – 463
Helixi87 – 10317
Helixi111 – 12717
Helixi131 – 14313
Helixi151 – 16818
Helixi176 – 1783
Helixi179 – 1835

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3W4YX-ray2.00A/B/C73-189[»]
4E0HX-ray2.00A86-189[»]
4E0IX-ray3.00A/B/C1-189[»]
ProteinModelPortaliP27882.
SMRiP27882. Positions 14-188.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini83 – 183101ERV/ALR sulfhydryl oxidase
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni166 – 18318FAD-binding By similarity
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG5054.
GeneTreeiENSGT00390000001979.
HOGENOMiHOG000195924.
KOiK17783.
OMAiYKEMASS.
OrthoDBiEOG7WDNCT.

Family and domain databases

Gene3Di1.20.120.310. 1 hit.
InterProiIPR017905. ERV/ALR_sulphydryl_oxidase.
[Graphical view]
PfamiPF04777. Evr1_Alr. 1 hit.
[Graphical view]
SUPFAMiSSF69000. SSF69000. 1 hit.
PROSITEiPS51324. ERV_ALR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P27882-1 [UniParc]FASTAAdd to Basket

« Hide

MKAIDKMTDN PPQEGLSGRK IIYDEDGKPC RSCNTLLDFQ YVTGKISNGL    50
KNLSSNGKLA GTGALTGEAS ELMPGSRTYR KVDPPDVEQL GRSSWTLLHS 100
VAASYPAQPT DQQKGEMKQF LNIFSHIYPC NWCAKDFEKY IRENAPQVES 150
REELGRWMCE AHNKVNKKLR KPKFDCNFWE KRWKDGWDE 189
Length:189
Mass (Da):21,639
Last modified:November 1, 1997 - v2
Checksum:i83DADA0DB1DF17CC
GO

Sequence cautioni

The sequence AAB48659.1 differs from that shown. Reason: Erroneous gene model prediction.
The sequence CAA43129.1 differs from that shown. Reason: Erroneous gene model prediction.
The sequence CAA48192.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X60722 Genomic DNA. Translation: CAA43129.1. Sequence problems.
M74772 Genomic DNA. Translation: AAB48659.1. Sequence problems.
X68055 Genomic DNA. Translation: CAA48192.1. Sequence problems.
Z72814 Genomic DNA. Translation: CAA97017.1.
Z72813 Genomic DNA. Translation: CAA97016.1.
BK006941 Genomic DNA. Translation: DAA08125.1.
PIRiS20469.
RefSeqiNP_011543.4. NM_001181158.3.

Genome annotation databases

EnsemblFungiiYGR029W; YGR029W; YGR029W.
GeneIDi852916.
KEGGisce:YGR029W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X60722 Genomic DNA. Translation: CAA43129.1 . Sequence problems.
M74772 Genomic DNA. Translation: AAB48659.1 . Sequence problems.
X68055 Genomic DNA. Translation: CAA48192.1 . Sequence problems.
Z72814 Genomic DNA. Translation: CAA97017.1 .
Z72813 Genomic DNA. Translation: CAA97016.1 .
BK006941 Genomic DNA. Translation: DAA08125.1 .
PIRi S20469.
RefSeqi NP_011543.4. NM_001181158.3.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3W4Y X-ray 2.00 A/B/C 73-189 [» ]
4E0H X-ray 2.00 A 86-189 [» ]
4E0I X-ray 3.00 A/B/C 1-189 [» ]
ProteinModelPortali P27882.
SMRi P27882. Positions 14-188.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33273. 22 interactions.
IntActi P27882. 5 interactions.
STRINGi 4932.YGR029W.

Proteomic databases

MaxQBi P27882.
PaxDbi P27882.
PeptideAtlasi P27882.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGR029W ; YGR029W ; YGR029W .
GeneIDi 852916.
KEGGi sce:YGR029W.

Organism-specific databases

CYGDi YGR029w.
SGDi S000003261. ERV1.

Phylogenomic databases

eggNOGi COG5054.
GeneTreei ENSGT00390000001979.
HOGENOMi HOG000195924.
KOi K17783.
OMAi YKEMASS.
OrthoDBi EOG7WDNCT.

Enzyme and pathway databases

BioCyci YEAST:G3O-30753-MONOMER.
Reactomei REACT_189012. Mitochondrial protein import.

Miscellaneous databases

NextBioi 972617.
PROi P27882.

Gene expression databases

Genevestigatori P27882.

Family and domain databases

Gene3Di 1.20.120.310. 1 hit.
InterProi IPR017905. ERV/ALR_sulphydryl_oxidase.
[Graphical view ]
Pfami PF04777. Evr1_Alr. 1 hit.
[Graphical view ]
SUPFAMi SSF69000. SSF69000. 1 hit.
PROSITEi PS51324. ERV_ALR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Dual function of a new nuclear gene for oxidative phosphorylation and vegetative growth in yeast."
    Lisowsky T.
    Mol. Gen. Genet. 232:58-64(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: SC167.
  2. "Intramitochondrial protein sorting. Isolation and characterization of the yeast MSP1 gene which belongs to a novel family of putative ATPases."
    Nakai M., Endo T., Hase T., Matsubara H.
    J. Biol. Chem. 268:24262-24269(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: SF747-19D.
  3. "Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII."
    Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.
    Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. "Removal of an intron with unique 3' branch site creates an amino-terminal protein sequence directing the scERV1 gene product to mitochondria."
    Lisowsky T.
    Yeast 12:1501-1510(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVISION OF GENE MODEL.
  7. "Erv1p from Saccharomyces cerevisiae is a FAD-linked sulfhydryl oxidase."
    Lee J.-E., Hofhaus G., Lisowsky T.
    FEBS Lett. 477:62-66(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, FAD-BINDING.
  8. "The N-terminal cysteine pair of yeast sulfhydryl oxidase Erv1p is essential for in vivo activity and interacts with the primary redox centre."
    Hofhaus G., Lee J.E., Tews I., Rosenberg B., Lisowsky T.
    Eur. J. Biochem. 270:1528-1535(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF CYS-30; CYS-33; CYS-130; CYS-133; CYS-159 AND CYS-176, SUBUNIT.
  9. "An essential function of the mitochondrial sulfhydryl oxidase Erv1p/ALR in the maturation of cytosolic Fe/S proteins."
    Lange H., Lisowsky T., Gerber J., Muhlenhoff U., Kispal G., Lill R.
    EMBO Rep. 2:715-720(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  10. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: ATCC 76625 / YPH499.
  11. "A disulfide relay system in the intermembrane space of mitochondria that mediates protein import."
    Mesecke N., Terziyska N., Kozany C., Baumann F., Neupert W., Hell K., Herrmann J.M.
    Cell 121:1059-1069(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH MIA40.
  12. "The essential mitochondrial protein Erv1 cooperates with Mia40 in biogenesis of intermembrane space proteins."
    Rissler M., Wiedemann N., Pfannschmidt S., Gabriel K., Guiard B., Pfanner N., Chacinska A.
    J. Mol. Biol. 353:485-492(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH MIA40.
  13. "Erv1 mediates the Mia40-dependent protein import pathway and provides a functional link to the respiratory chain by shuttling electrons to cytochrome c."
    Allen S., Balabanidou V., Sideris D.P., Lisowsky T., Tokatlidis K.
    J. Mol. Biol. 353:937-944(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  14. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiERV1_YEAST
AccessioniPrimary (citable) accession number: P27882
Secondary accession number(s): D6VUG4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: November 1, 1997
Last modified: September 3, 2014
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

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