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P27882 (ERV1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 123. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitochondrial FAD-linked sulfhydryl oxidase ERV1

EC=1.8.3.2
Alternative name(s):
14 kDa regulatory protein
Essential for respiration and vegetative growth protein 1
Gene names
Name:ERV1
Ordered Locus Names:YGR029W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length189 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

FAD-dependent sulfhydryl oxidase that catalyzes disulfide bond formation. Required for the import and folding of small cysteine-containing proteins in the mitochondrial intermembrane space (IMS). Forms a redox cycle with MIA40 that involves a disulfide relay system. Important for maintaining the cysteine residues in MIA40 in an oxidized state. Reduced ERV1 is reoxidized by cytochrome c. Required for the maturation of cytoplasmic, but not of mitochondrial Fe/S proteins. Ref.7 Ref.8 Ref.9 Ref.11 Ref.12 Ref.13

Catalytic activity

2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2.

Cofactor

FAD.

Subunit structure

Homodimer. Interacts with MIA40, forming transient intermolecular disulfide bridges. Ref.8 Ref.11 Ref.12

Subcellular location

Mitochondrion intermembrane space Ref.9 Ref.10.

Sequence similarities

Contains 1 ERV/ALR sulfhydryl oxidase domain.

Sequence caution

The sequence AAB48659.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence CAA43129.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence CAA48192.1 differs from that shown. Reason: Erroneous gene model prediction.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

MIA40P360462EBI-6621,EBI-26978

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 189189Mitochondrial FAD-linked sulfhydryl oxidase ERV1
PRO_0000001187

Regions

Domain83 – 183101ERV/ALR sulfhydryl oxidase
Region166 – 18318FAD-binding By similarity

Amino acid modifications

Disulfide bond130 ↔ 133Redox-active By similarity
Disulfide bond159 ↔ 176 By similarity

Experimental info

Mutagenesis301C → S: Reduces catalytic activity 3-fold. Ref.8
Mutagenesis331C → S: Reduces catalytic activity 2-fold. Ref.8
Mutagenesis1301C → S: Loss of function. Ref.8
Mutagenesis1331C → S: Loss of function. Ref.8
Mutagenesis1591C → S: Reduces catalytic activity 3.3-fold. Ref.8
Mutagenesis1761C → S: Reduces catalytic activity 2.6-fold. Ref.8

Secondary structure

................... 189
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P27882 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: 83DADA0DB1DF17CC

FASTA18921,639
        10         20         30         40         50         60 
MKAIDKMTDN PPQEGLSGRK IIYDEDGKPC RSCNTLLDFQ YVTGKISNGL KNLSSNGKLA 

        70         80         90        100        110        120 
GTGALTGEAS ELMPGSRTYR KVDPPDVEQL GRSSWTLLHS VAASYPAQPT DQQKGEMKQF 

       130        140        150        160        170        180 
LNIFSHIYPC NWCAKDFEKY IRENAPQVES REELGRWMCE AHNKVNKKLR KPKFDCNFWE 


KRWKDGWDE 

« Hide

References

« Hide 'large scale' references
[1]"Dual function of a new nuclear gene for oxidative phosphorylation and vegetative growth in yeast."
Lisowsky T.
Mol. Gen. Genet. 232:58-64(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: SC167.
[2]"Intramitochondrial protein sorting. Isolation and characterization of the yeast MSP1 gene which belongs to a novel family of putative ATPases."
Nakai M., Endo T., Hase T., Matsubara H.
J. Biol. Chem. 268:24262-24269(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: SF747-19D.
[3]"Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII."
Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.
Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[4]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[6]"Removal of an intron with unique 3' branch site creates an amino-terminal protein sequence directing the scERV1 gene product to mitochondria."
Lisowsky T.
Yeast 12:1501-1510(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: REVISION OF GENE MODEL.
[7]"Erv1p from Saccharomyces cerevisiae is a FAD-linked sulfhydryl oxidase."
Lee J.-E., Hofhaus G., Lisowsky T.
FEBS Lett. 477:62-66(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, FAD-BINDING.
[8]"The N-terminal cysteine pair of yeast sulfhydryl oxidase Erv1p is essential for in vivo activity and interacts with the primary redox centre."
Hofhaus G., Lee J.E., Tews I., Rosenberg B., Lisowsky T.
Eur. J. Biochem. 270:1528-1535(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF CYS-30; CYS-33; CYS-130; CYS-133; CYS-159 AND CYS-176, SUBUNIT.
[9]"An essential function of the mitochondrial sulfhydryl oxidase Erv1p/ALR in the maturation of cytosolic Fe/S proteins."
Lange H., Lisowsky T., Gerber J., Muhlenhoff U., Kispal G., Lill R.
EMBO Rep. 2:715-720(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[10]"The proteome of Saccharomyces cerevisiae mitochondria."
Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C.
Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Strain: ATCC 76625 / YPH499.
[11]"A disulfide relay system in the intermembrane space of mitochondria that mediates protein import."
Mesecke N., Terziyska N., Kozany C., Baumann F., Neupert W., Hell K., Herrmann J.M.
Cell 121:1059-1069(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH MIA40.
[12]"The essential mitochondrial protein Erv1 cooperates with Mia40 in biogenesis of intermembrane space proteins."
Rissler M., Wiedemann N., Pfannschmidt S., Gabriel K., Guiard B., Pfanner N., Chacinska A.
J. Mol. Biol. 353:485-492(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH MIA40.
[13]"Erv1 mediates the Mia40-dependent protein import pathway and provides a functional link to the respiratory chain by shuttling electrons to cytochrome c."
Allen S., Balabanidou V., Sideris D.P., Lisowsky T., Tokatlidis K.
J. Mol. Biol. 353:937-944(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[14]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X60722 Genomic DNA. Translation: CAA43129.1. Sequence problems.
M74772 Genomic DNA. Translation: AAB48659.1. Sequence problems.
X68055 Genomic DNA. Translation: CAA48192.1. Sequence problems.
Z72814 Genomic DNA. Translation: CAA97017.1.
Z72813 Genomic DNA. Translation: CAA97016.1.
BK006941 Genomic DNA. Translation: DAA08125.1.
PIRS20469.
RefSeqNP_011543.4. NM_001181158.3.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3W4YX-ray2.00A/B/C73-189[»]
4E0HX-ray2.00A86-189[»]
4E0IX-ray3.00A/B/C1-189[»]
ProteinModelPortalP27882.
SMRP27882. Positions 14-188.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33273. 22 interactions.
IntActP27882. 5 interactions.
STRING4932.YGR029W.

Proteomic databases

MaxQBP27882.
PaxDbP27882.
PeptideAtlasP27882.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGR029W; YGR029W; YGR029W.
GeneID852916.
KEGGsce:YGR029W.

Organism-specific databases

CYGDYGR029w.
SGDS000003261. ERV1.

Phylogenomic databases

eggNOGCOG5054.
GeneTreeENSGT00390000001979.
HOGENOMHOG000195924.
KOK17783.
OMAYKEMASS.
OrthoDBEOG7WDNCT.

Enzyme and pathway databases

BioCycYEAST:G3O-30753-MONOMER.
ReactomeREACT_118590. Mitochondrial Protein Import (yeast).

Gene expression databases

GenevestigatorP27882.

Family and domain databases

Gene3D1.20.120.310. 1 hit.
InterProIPR017905. ERV/ALR_sulphydryl_oxidase.
[Graphical view]
PfamPF04777. Evr1_Alr. 1 hit.
[Graphical view]
SUPFAMSSF69000. SSF69000. 1 hit.
PROSITEPS51324. ERV_ALR. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio972617.
PROP27882.

Entry information

Entry nameERV1_YEAST
AccessionPrimary (citable) accession number: P27882
Secondary accession number(s): D6VUG4
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: November 1, 1997
Last modified: June 11, 2014
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references