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Protein

Mitochondrial FAD-linked sulfhydryl oxidase ERV1

Gene

ERV1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

FAD-dependent sulfhydryl oxidase that catalyzes disulfide bond formation. Required for the import and folding of small cysteine-containing proteins in the mitochondrial intermembrane space (IMS). Forms a redox cycle with MIA40 that involves a disulfide relay system. Important for maintaining the cysteine residues in MIA40 in an oxidized state. Reduced ERV1 is reoxidized by cytochrome c. Required for the maturation of cytoplasmic, but not of mitochondrial Fe/S proteins.PROSITE-ProRule annotation6 Publications

Catalytic activityi

2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2.

Cofactori

FADPROSITE-ProRule annotation1 Publication

GO - Molecular functioni

  • flavin adenine dinucleotide binding Source: GO_Central
  • flavin-linked sulfhydryl oxidase activity Source: SGD
  • protein disulfide oxidoreductase activity Source: GO_Central
  • thiol oxidase activity Source: SGD

GO - Biological processi

  • cellular iron ion homeostasis Source: SGD
  • cellular response to oxidative stress Source: SGD
  • protein import into mitochondrial intermembrane space Source: SGD
  • protein targeting to mitochondrion Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciYEAST:G3O-30753-MONOMER.
BRENDAi1.8.3.2. 984.
ReactomeiR-SCE-1268020. Mitochondrial protein import.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial FAD-linked sulfhydryl oxidase ERV1 (EC:1.8.3.2)
Alternative name(s):
14 kDa regulatory protein
Essential for respiration and vegetative growth protein 1
Gene namesi
Name:ERV1
Ordered Locus Names:YGR029W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR029W.
SGDiS000003261. ERV1.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial intermembrane space Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi30C → S: Reduces catalytic activity 3-fold. 1 Publication1
Mutagenesisi33C → S: Reduces catalytic activity 2-fold. 1 Publication1
Mutagenesisi130C → S: Loss of function. 1 Publication1
Mutagenesisi133C → S: Loss of function. 1 Publication1
Mutagenesisi159C → S: Reduces catalytic activity 3.3-fold. 1 Publication1
Mutagenesisi176C → S: Reduces catalytic activity 2.6-fold. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000011871 – 189Mitochondrial FAD-linked sulfhydryl oxidase ERV1Add BLAST189

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi130 ↔ 133Redox-activePROSITE-ProRule annotation
Disulfide bondi159 ↔ 176PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiP27882.
PRIDEiP27882.

Interactioni

Subunit structurei

Homodimer. Interacts with MIA40, forming transient intermolecular disulfide bridges.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MIA40P360462EBI-6621,EBI-26978

Protein-protein interaction databases

BioGridi33273. 23 interactors.
IntActiP27882. 5 interactors.

Structurei

Secondary structure

1189
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi16 – 18Combined sources3
Beta strandi26 – 29Combined sources4
Helixi36 – 43Combined sources8
Turni44 – 46Combined sources3
Helixi87 – 103Combined sources17
Helixi111 – 127Combined sources17
Helixi131 – 143Combined sources13
Helixi151 – 168Combined sources18
Helixi176 – 178Combined sources3
Helixi179 – 183Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3W4YX-ray2.00A/B/C73-189[»]
4E0HX-ray2.00A86-189[»]
4E0IX-ray3.00A/B/C1-189[»]
ProteinModelPortaliP27882.
SMRiP27882.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini83 – 183ERV/ALR sulfhydryl oxidasePROSITE-ProRule annotationAdd BLAST101

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni166 – 183FAD-bindingBy similarityAdd BLAST18

Sequence similaritiesi

Contains 1 ERV/ALR sulfhydryl oxidase domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00390000001979.
HOGENOMiHOG000195924.
InParanoidiP27882.
KOiK17783.
OMAiKKRWKDG.
OrthoDBiEOG092C3SAY.

Family and domain databases

Gene3Di1.20.120.310. 1 hit.
InterProiIPR017905. ERV/ALR_sulphydryl_oxidase.
[Graphical view]
PfamiPF04777. Evr1_Alr. 1 hit.
[Graphical view]
SUPFAMiSSF69000. SSF69000. 1 hit.
PROSITEiPS51324. ERV_ALR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P27882-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAIDKMTDN PPQEGLSGRK IIYDEDGKPC RSCNTLLDFQ YVTGKISNGL
60 70 80 90 100
KNLSSNGKLA GTGALTGEAS ELMPGSRTYR KVDPPDVEQL GRSSWTLLHS
110 120 130 140 150
VAASYPAQPT DQQKGEMKQF LNIFSHIYPC NWCAKDFEKY IRENAPQVES
160 170 180
REELGRWMCE AHNKVNKKLR KPKFDCNFWE KRWKDGWDE
Length:189
Mass (Da):21,639
Last modified:November 1, 1997 - v2
Checksum:i83DADA0DB1DF17CC
GO

Sequence cautioni

The sequence AAB48659 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAA43129 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAA48192 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60722 Genomic DNA. Translation: CAA43129.1. Sequence problems.
M74772 Genomic DNA. Translation: AAB48659.1. Sequence problems.
X68055 Genomic DNA. Translation: CAA48192.1. Sequence problems.
Z72814 Genomic DNA. Translation: CAA97017.1.
Z72813 Genomic DNA. Translation: CAA97016.1.
BK006941 Genomic DNA. Translation: DAA08125.1.
PIRiS20469.
RefSeqiNP_011543.4. NM_001181158.3.

Genome annotation databases

EnsemblFungiiYGR029W; YGR029W; YGR029W.
GeneIDi852916.
KEGGisce:YGR029W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60722 Genomic DNA. Translation: CAA43129.1. Sequence problems.
M74772 Genomic DNA. Translation: AAB48659.1. Sequence problems.
X68055 Genomic DNA. Translation: CAA48192.1. Sequence problems.
Z72814 Genomic DNA. Translation: CAA97017.1.
Z72813 Genomic DNA. Translation: CAA97016.1.
BK006941 Genomic DNA. Translation: DAA08125.1.
PIRiS20469.
RefSeqiNP_011543.4. NM_001181158.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3W4YX-ray2.00A/B/C73-189[»]
4E0HX-ray2.00A86-189[»]
4E0IX-ray3.00A/B/C1-189[»]
ProteinModelPortaliP27882.
SMRiP27882.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33273. 23 interactors.
IntActiP27882. 5 interactors.

Proteomic databases

MaxQBiP27882.
PRIDEiP27882.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR029W; YGR029W; YGR029W.
GeneIDi852916.
KEGGisce:YGR029W.

Organism-specific databases

EuPathDBiFungiDB:YGR029W.
SGDiS000003261. ERV1.

Phylogenomic databases

GeneTreeiENSGT00390000001979.
HOGENOMiHOG000195924.
InParanoidiP27882.
KOiK17783.
OMAiKKRWKDG.
OrthoDBiEOG092C3SAY.

Enzyme and pathway databases

BioCyciYEAST:G3O-30753-MONOMER.
BRENDAi1.8.3.2. 984.
ReactomeiR-SCE-1268020. Mitochondrial protein import.

Miscellaneous databases

PROiP27882.

Family and domain databases

Gene3Di1.20.120.310. 1 hit.
InterProiIPR017905. ERV/ALR_sulphydryl_oxidase.
[Graphical view]
PfamiPF04777. Evr1_Alr. 1 hit.
[Graphical view]
SUPFAMiSSF69000. SSF69000. 1 hit.
PROSITEiPS51324. ERV_ALR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiERV1_YEAST
AccessioniPrimary (citable) accession number: P27882
Secondary accession number(s): D6VUG4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 143 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.