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Protein

Mitochondrial FAD-linked sulfhydryl oxidase ERV1

Gene

ERV1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

FAD-dependent sulfhydryl oxidase that catalyzes disulfide bond formation. Required for the import and folding of small cysteine-containing proteins in the mitochondrial intermembrane space (IMS). Forms a redox cycle with MIA40 that involves a disulfide relay system. Important for maintaining the cysteine residues in MIA40 in an oxidized state. Reduced ERV1 is reoxidized by cytochrome c. Required for the maturation of cytoplasmic, but not of mitochondrial Fe/S proteins.PROSITE-ProRule annotation6 Publications

Catalytic activityi

2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2.

Cofactori

FADPROSITE-ProRule annotation1 Publication

GO - Molecular functioni

  • flavin adenine dinucleotide binding Source: GO_Central
  • flavin-linked sulfhydryl oxidase activity Source: SGD
  • protein disulfide oxidoreductase activity Source: GO_Central
  • thiol oxidase activity Source: SGD

GO - Biological processi

  • cellular iron ion homeostasis Source: SGD
  • cellular response to oxidative stress Source: SGD
  • protein import into mitochondrial intermembrane space Source: SGD

Keywordsi

Molecular functionOxidoreductase
LigandFAD, Flavoprotein

Enzyme and pathway databases

BioCyciYEAST:G3O-30753-MONOMER
BRENDAi1.8.3.2 984

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial FAD-linked sulfhydryl oxidase ERV1 (EC:1.8.3.2)
Alternative name(s):
14 kDa regulatory protein
Essential for respiration and vegetative growth protein 1
Gene namesi
Name:ERV1
Ordered Locus Names:YGR029W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi

Organism-specific databases

EuPathDBiFungiDB:YGR029W
SGDiS000003261 ERV1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi30C → S: Reduces catalytic activity 3-fold. 1 Publication1
Mutagenesisi33C → S: Reduces catalytic activity 2-fold. 1 Publication1
Mutagenesisi130C → S: Loss of function. 1 Publication1
Mutagenesisi133C → S: Loss of function. 1 Publication1
Mutagenesisi159C → S: Reduces catalytic activity 3.3-fold. 1 Publication1
Mutagenesisi176C → S: Reduces catalytic activity 2.6-fold. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000011871 – 189Mitochondrial FAD-linked sulfhydryl oxidase ERV1Add BLAST189

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi130 ↔ 133Redox-activePROSITE-ProRule annotation
Disulfide bondi159 ↔ 176PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiP27882
PaxDbiP27882
PRIDEiP27882

PTM databases

CarbonylDBiP27882

Interactioni

Subunit structurei

Homodimer. Interacts with MIA40, forming transient intermolecular disulfide bridges.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MIA40P360464EBI-6621,EBI-26978

Protein-protein interaction databases

BioGridi33273, 26 interactors
IntActiP27882, 5 interactors
MINTiP27882
STRINGi4932.YGR029W

Structurei

Secondary structure

1189
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi16 – 18Combined sources3
Beta strandi26 – 29Combined sources4
Helixi36 – 43Combined sources8
Turni44 – 46Combined sources3
Helixi87 – 103Combined sources17
Helixi111 – 127Combined sources17
Helixi131 – 143Combined sources13
Helixi151 – 168Combined sources18
Helixi176 – 178Combined sources3
Helixi179 – 183Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3W4YX-ray2.00A/B/C73-189[»]
4E0HX-ray2.00A86-189[»]
4E0IX-ray3.00A/B/C1-189[»]
ProteinModelPortaliP27882
SMRiP27882
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini83 – 183ERV/ALR sulfhydryl oxidasePROSITE-ProRule annotationAdd BLAST101

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni166 – 183FAD-bindingBy similarityAdd BLAST18

Phylogenomic databases

GeneTreeiENSGT00390000001979
HOGENOMiHOG000195924
InParanoidiP27882
KOiK17783
OMAiLCDAHNE
OrthoDBiEOG092C3SAY

Family and domain databases

Gene3Di1.20.120.310, 1 hit
InterProiView protein in InterPro
IPR036774 ERV/ALR_sulphydryl_oxid_sf
IPR017905 ERV/ALR_sulphydryl_oxidase
PfamiView protein in Pfam
PF04777 Evr1_Alr, 1 hit
SUPFAMiSSF69000 SSF69000, 1 hit
PROSITEiView protein in PROSITE
PS51324 ERV_ALR, 1 hit

Sequencei

Sequence statusi: Complete.

P27882-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAIDKMTDN PPQEGLSGRK IIYDEDGKPC RSCNTLLDFQ YVTGKISNGL
60 70 80 90 100
KNLSSNGKLA GTGALTGEAS ELMPGSRTYR KVDPPDVEQL GRSSWTLLHS
110 120 130 140 150
VAASYPAQPT DQQKGEMKQF LNIFSHIYPC NWCAKDFEKY IRENAPQVES
160 170 180
REELGRWMCE AHNKVNKKLR KPKFDCNFWE KRWKDGWDE
Length:189
Mass (Da):21,639
Last modified:November 1, 1997 - v2
Checksum:i83DADA0DB1DF17CC
GO

Sequence cautioni

The sequence AAB48659 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAA43129 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAA48192 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60722 Genomic DNA Translation: CAA43129.1 Sequence problems.
M74772 Genomic DNA Translation: AAB48659.1 Sequence problems.
X68055 Genomic DNA Translation: CAA48192.1 Sequence problems.
Z72814 Genomic DNA Translation: CAA97017.1
Z72813 Genomic DNA Translation: CAA97016.1
BK006941 Genomic DNA Translation: DAA08125.1
PIRiS20469
RefSeqiNP_011543.4, NM_001181158.3

Genome annotation databases

EnsemblFungiiYGR029W; YGR029W; YGR029W
GeneIDi852916
KEGGisce:YGR029W

Entry informationi

Entry nameiERV1_YEAST
AccessioniPrimary (citable) accession number: P27882
Secondary accession number(s): D6VUG4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: November 1, 1997
Last modified: May 23, 2018
This is version 155 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

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