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P27881

- HXK2_RAT

UniProt

P27881 - HXK2_RAT

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Protein

Hexokinase-2

Gene

Hk2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + D-hexose = ADP + D-hexose 6-phosphate.

Enzyme regulationi

Hexokinase is an allosteric enzyme inhibited by its product Glc-6-P.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei30 – 301ATP 1By similarity
Binding sitei209 – 2091Glucose-6-phosphate 1By similarity
Binding sitei232 – 2321Glucose-6-phosphate 1By similarity
Binding sitei235 – 2351Substrate 1By similarity
Binding sitei260 – 2601Substrate 1By similarity
Binding sitei449 – 4491Glucose-6-phosphate 1By similarity
Binding sitei558 – 5581ATP 2Sequence Analysis
Binding sitei657 – 6571Glucose-6-phosphate 2By similarity
Binding sitei680 – 6801ATP 2By similarity
Binding sitei680 – 6801Glucose-6-phosphate 2By similarity
Binding sitei683 – 6831Substrate 2By similarity
Binding sitei708 – 7081Substrate 2By similarity
Binding sitei897 – 8971Glucose-6-phosphate 2By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi84 – 896ATP 1Sequence Analysis
Nucleotide bindingi425 – 4262ATP 1By similarity
Nucleotide bindingi532 – 5376ATP 2By similarity
Nucleotide bindingi747 – 7482ATP 2By similarity
Nucleotide bindingi784 – 7885ATP 2By similarity
Nucleotide bindingi863 – 8675ATP 2By similarity

GO - Molecular functioni

  1. ATP binding Source: RGD
  2. fructokinase activity Source: RefGenome
  3. glucokinase activity Source: RefGenome
  4. glucose binding Source: RGD
  5. hexokinase activity Source: RGD
  6. mannokinase activity Source: RefGenome

GO - Biological processi

  1. apoptotic mitochondrial changes Source: Ensembl
  2. carbohydrate phosphorylation Source: RGD
  3. cellular glucose homeostasis Source: RefGenome
  4. glucose 6-phosphate metabolic process Source: GOC
  5. glucose catabolic process Source: RGD
  6. glycolytic process Source: RefGenome
  7. lactation Source: RGD
  8. regulation of glucose import Source: Ensembl
  9. response to ischemia Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.1. 5301.
ReactomeiREACT_205960. Glucose transport.
SABIO-RKP27881.
UniPathwayiUPA00242.

Names & Taxonomyi

Protein namesi
Recommended name:
Hexokinase-2 (EC:2.7.1.1)
Alternative name(s):
Hexokinase type II
Short name:
HK II
Gene namesi
Name:Hk2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 4

Organism-specific databases

RGDi2797. Hk2.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: RefGenome
  2. mitochondrial outer membrane Source: RGD
  3. mitochondrion Source: RGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 917917Hexokinase-2PRO_0000197589Add
BLAST

Proteomic databases

PaxDbiP27881.
PRIDEiP27881.

PTM databases

PhosphoSiteiP27881.

Expressioni

Gene expression databases

GenevestigatoriP27881.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi247136. 3 interactions.
DIPiDIP-37314N.
IntActiP27881. 1 interaction.
MINTiMINT-1775746.
STRINGi10116.ENSRNOP00000008813.

Structurei

3D structure databases

ProteinModelPortaliP27881.
SMRiP27881. Positions 17-913.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini17 – 221205Hexokinase type-1 1Add
BLAST
Domaini223 – 462240Hexokinase type-2 1Add
BLAST
Domaini465 – 669205Hexokinase type-1 2Add
BLAST
Domaini671 – 910240Hexokinase type-2 2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 1212HydrophobicAdd
BLAST
Regioni13 – 475463RegulatoryAdd
BLAST
Regioni84 – 885Glucose-6-phosphate 1 bindingBy similarity
Regioni155 – 1562Substrate 1 bindingBy similarity
Regioni172 – 1732Substrate 1 bindingBy similarity
Regioni208 – 2092Substrate 1 bindingBy similarity
Regioni291 – 2944Substrate 1 bindingBy similarity
Regioni413 – 4153Glucose-6-phosphate 1 bindingBy similarity
Regioni476 – 917442CatalyticAdd
BLAST
Regioni532 – 5365Glucose-6-phosphate 2 bindingBy similarity
Regioni603 – 6042Substrate 2 bindingBy similarity
Regioni620 – 6212Substrate 2 bindingBy similarity
Regioni656 – 6572Substrate 2 bindingBy similarity
Regioni739 – 7424Substrate 2 bindingBy similarity
Regioni861 – 8633Glucose-6-phosphate 2 bindingBy similarity

Domaini

The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus.

Sequence similaritiesi

Belongs to the hexokinase family.Curated
Contains 2 hexokinase type-1 domains.Curated
Contains 2 hexokinase type-2 domains.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG5026.
GeneTreeiENSGT00390000017159.
HOGENOMiHOG000162671.
HOVERGENiHBG005020.
InParanoidiP27881.
KOiK00844.
OMAiHHLGLES.
OrthoDBiEOG7S21X5.
PhylomeDBiP27881.
TreeFamiTF314238.

Family and domain databases

InterProiIPR001312. Hexokinase.
IPR022673. Hexokinase_C.
IPR019807. Hexokinase_CS.
IPR022672. Hexokinase_N.
[Graphical view]
PANTHERiPTHR19443. PTHR19443. 1 hit.
PfamiPF00349. Hexokinase_1. 2 hits.
PF03727. Hexokinase_2. 2 hits.
[Graphical view]
PRINTSiPR00475. HEXOKINASE.
PROSITEiPS00378. HEXOKINASES. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P27881-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIASHMIACL FTELNQNQVQ KVDQFLYHMR LSDETLLEIS RRFRKEMEKG
60 70 80 90 100
LGATTHPTAA VKMLPTFVRS TPDGTEHGEF LALDLGGTNF RVLRVRVTDN
110 120 130 140 150
GLQRVEMENQ IYAIPEDIMR GSGTQLFDHI AECLANFMDK LQIKEKKLPL
160 170 180 190 200
GFTFSFPCHQ TKLDESFLVS WTKGFKSSGV EGRDVVDLIR KAIQRRGDFD
210 220 230 240 250
IDIVAVVNDT VGTMMTCGYD DQNCEIGLIV GTGSNACYME EMRHIDMVEG
260 270 280 290 300
DEGRMCINME WGAFGDDGTL NDIRTEFDRE IDMGSLNPGK QLFEKMISGM
310 320 330 340 350
YMGELVRLIL VKMAKAELLF QGKLSPELLT TGSFETKDVS DIEEDKDGIE
360 370 380 390 400
KAYQILMRLG LNPLQEDCVA THRICQIVST RSASLCAATL AAVLWRIKEN
410 420 430 440 450
KGEERLRSTI GVDGSVYKKH PHFAKRLHKA VRRLVPDCDV RFLRSEDGSG
460 470 480 490 500
KGAAMVTAVA YRLADQHRAR QKTLESLKLS HEQLLEVKRR MKVEMEQGLS
510 520 530 540 550
KETHAVAPVK MLPTYVCATP DGTEKGDFLA LDLGGTNFRV LLVRVRNGKR
560 570 580 590 600
RGVEMHNKIY SIPQEVMHGT GEELFDHIVQ CIADFLEYMG MKGVSLPLGF
610 620 630 640 650
TFSFPCQQNS LDQSILLKWT KGFKASGCEG EDVVTLLKEA IHRREEFDLD
660 670 680 690 700
VVAVVNDTVG TMMTCGYEDP HCEVGLIVGT GSNACYMEEM RNVELVDGEE
710 720 730 740 750
GRMCVNMEWG AFGDNGCLDD LRTVFDVAVD ELSLNPGKQR FEKMISGMYL
760 770 780 790 800
GEIVRNILID FTKRGLLFRG RISERLKTRG IFETKFLSQI ESDCLALLQV
810 820 830 840 850
RAILRHLGLE STCDDSIIVK EVCTVVARRA AQLCGAGMAA VVDKIRENRG
860 870 880 890 900
LDNLKVTVGV DGTLYKLHPH FAKVMHETVR DLAPKCDVSF LESEDGSGKG
910
AALITAVACR IREAGQR
Length:917
Mass (Da):102,544
Last modified:August 1, 1992 - v1
Checksum:i764EC189C0F90987
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M68971 mRNA. Translation: AAA41333.1.
M68972 mRNA. Translation: AAA41334.1.
D26393 Genomic DNA. Translation: BAA05409.1.
U19605 Genomic DNA. Translation: AAB09025.1.
AY082375 Genomic DNA. Translation: AAL92551.1.
PIRiS15885.
RefSeqiNP_036867.1. NM_012735.2.
UniGeneiRn.91375.

Genome annotation databases

EnsembliENSRNOT00000008813; ENSRNOP00000008813; ENSRNOG00000006116.
GeneIDi25059.
KEGGirno:25059.
UCSCiRGD:2797. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M68971 mRNA. Translation: AAA41333.1 .
M68972 mRNA. Translation: AAA41334.1 .
D26393 Genomic DNA. Translation: BAA05409.1 .
U19605 Genomic DNA. Translation: AAB09025.1 .
AY082375 Genomic DNA. Translation: AAL92551.1 .
PIRi S15885.
RefSeqi NP_036867.1. NM_012735.2.
UniGenei Rn.91375.

3D structure databases

ProteinModelPortali P27881.
SMRi P27881. Positions 17-913.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 247136. 3 interactions.
DIPi DIP-37314N.
IntActi P27881. 1 interaction.
MINTi MINT-1775746.
STRINGi 10116.ENSRNOP00000008813.

Chemistry

BindingDBi P27881.
ChEMBLi CHEMBL5063.

PTM databases

PhosphoSitei P27881.

Proteomic databases

PaxDbi P27881.
PRIDEi P27881.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000008813 ; ENSRNOP00000008813 ; ENSRNOG00000006116 .
GeneIDi 25059.
KEGGi rno:25059.
UCSCi RGD:2797. rat.

Organism-specific databases

CTDi 3099.
RGDi 2797. Hk2.

Phylogenomic databases

eggNOGi COG5026.
GeneTreei ENSGT00390000017159.
HOGENOMi HOG000162671.
HOVERGENi HBG005020.
InParanoidi P27881.
KOi K00844.
OMAi HHLGLES.
OrthoDBi EOG7S21X5.
PhylomeDBi P27881.
TreeFami TF314238.

Enzyme and pathway databases

UniPathwayi UPA00242 .
BRENDAi 2.7.1.1. 5301.
Reactomei REACT_205960. Glucose transport.
SABIO-RK P27881.

Miscellaneous databases

NextBioi 605276.
PROi P27881.

Gene expression databases

Genevestigatori P27881.

Family and domain databases

InterProi IPR001312. Hexokinase.
IPR022673. Hexokinase_C.
IPR019807. Hexokinase_CS.
IPR022672. Hexokinase_N.
[Graphical view ]
PANTHERi PTHR19443. PTHR19443. 1 hit.
Pfami PF00349. Hexokinase_1. 2 hits.
PF03727. Hexokinase_2. 2 hits.
[Graphical view ]
PRINTSi PR00475. HEXOKINASE.
PROSITEi PS00378. HEXOKINASES. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete amino acid sequence of the type II isozyme of rat hexokinase, deduced from the cloned cDNA: comparison with a hexokinase from novikoff ascites tumor."
    Thelen A.P., Wilson J.E.
    Arch. Biochem. Biophys. 286:645-651(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 11-18.
    Tissue: Skeletal muscle.
  2. "Nucleotide sequence of the 5'-flanking region of the rat type II hexokinase gene."
    Shinohara Y., Ichihara J., Kogure K., Terada H.
    Biochim. Biophys. Acta 1260:365-368(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-21.
    Strain: Wistar.
  3. "Glucose catabolism in cancer cells. Isolation, sequence, and activity of the promoter for type II hexokinase."
    Mathupala S.P., Rempel A., Pedersen P.L.
    J. Biol. Chem. 270:16918-16925(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-21.
    Strain: Sprague-Dawley.
    Tissue: Hepatoma.
  4. "Normal type II hexokinase promoter, first exon, and first intron from hepatocytes."
    Rempel A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-21.
    Strain: Sprague-Dawley.

Entry informationi

Entry nameiHXK2_RAT
AccessioniPrimary (citable) accession number: P27881
Secondary accession number(s): Q9QWR1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: October 1, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase).

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3