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P27881

- HXK2_RAT

UniProt

P27881 - HXK2_RAT

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Protein

Hexokinase-2

Gene
Hk2
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + D-hexose = ADP + D-hexose 6-phosphate.

Enzyme regulationi

Hexokinase is an allosteric enzyme inhibited by its product Glc-6-P.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei30 – 301ATP 1 By similarity
Binding sitei209 – 2091Glucose-6-phosphate 1 By similarity
Binding sitei232 – 2321Glucose-6-phosphate 1 By similarity
Binding sitei235 – 2351Substrate 1 By similarity
Binding sitei260 – 2601Substrate 1 By similarity
Binding sitei449 – 4491Glucose-6-phosphate 1 By similarity
Binding sitei558 – 5581ATP 2 Reviewed prediction
Binding sitei657 – 6571Glucose-6-phosphate 2 By similarity
Binding sitei680 – 6801ATP 2 By similarity
Binding sitei680 – 6801Glucose-6-phosphate 2 By similarity
Binding sitei683 – 6831Substrate 2 By similarity
Binding sitei708 – 7081Substrate 2 By similarity
Binding sitei897 – 8971Glucose-6-phosphate 2 By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi84 – 896ATP 1 Reviewed prediction
Nucleotide bindingi425 – 4262ATP 1 By similarity
Nucleotide bindingi532 – 5376ATP 2 By similarity
Nucleotide bindingi747 – 7482ATP 2 By similarity
Nucleotide bindingi784 – 7885ATP 2 By similarity
Nucleotide bindingi863 – 8675ATP 2 By similarity

GO - Molecular functioni

  1. ATP binding Source: RGD
  2. fructokinase activity Source: RefGenome
  3. glucokinase activity Source: RefGenome
  4. glucose binding Source: RGD
  5. hexokinase activity Source: RGD
  6. mannokinase activity Source: RefGenome

GO - Biological processi

  1. apoptotic mitochondrial changes Source: Ensembl
  2. carbohydrate phosphorylation Source: RGD
  3. cellular glucose homeostasis Source: RefGenome
  4. glucose 6-phosphate metabolic process Source: GOC
  5. glucose catabolic process Source: RGD
  6. glycolytic process Source: RefGenome
  7. lactation Source: RGD
  8. regulation of glucose import Source: Ensembl
  9. response to ischemia Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.1. 5301.
ReactomeiREACT_205960. Glucose transport.
SABIO-RKP27881.
UniPathwayiUPA00242.

Names & Taxonomyi

Protein namesi
Recommended name:
Hexokinase-2 (EC:2.7.1.1)
Alternative name(s):
Hexokinase type II
Short name:
HK II
Gene namesi
Name:Hk2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 4

Organism-specific databases

RGDi2797. Hk2.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: RefGenome
  2. mitochondrial outer membrane Source: RGD
  3. mitochondrion Source: RGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 917917Hexokinase-2PRO_0000197589Add
BLAST

Proteomic databases

PaxDbiP27881.
PRIDEiP27881.

PTM databases

PhosphoSiteiP27881.

Expressioni

Gene expression databases

GenevestigatoriP27881.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi247136. 3 interactions.
DIPiDIP-37314N.
IntActiP27881. 1 interaction.
MINTiMINT-1775746.
STRINGi10116.ENSRNOP00000008813.

Structurei

3D structure databases

ProteinModelPortaliP27881.
SMRiP27881. Positions 17-913.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini17 – 221205Hexokinase type-1 1Add
BLAST
Domaini223 – 462240Hexokinase type-2 1Add
BLAST
Domaini465 – 669205Hexokinase type-1 2Add
BLAST
Domaini671 – 910240Hexokinase type-2 2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 1212HydrophobicAdd
BLAST
Regioni13 – 475463RegulatoryAdd
BLAST
Regioni84 – 885Glucose-6-phosphate 1 binding By similarity
Regioni155 – 1562Substrate 1 binding By similarity
Regioni172 – 1732Substrate 1 binding By similarity
Regioni208 – 2092Substrate 1 binding By similarity
Regioni291 – 2944Substrate 1 binding By similarity
Regioni413 – 4153Glucose-6-phosphate 1 binding By similarity
Regioni476 – 917442CatalyticAdd
BLAST
Regioni532 – 5365Glucose-6-phosphate 2 binding By similarity
Regioni603 – 6042Substrate 2 binding By similarity
Regioni620 – 6212Substrate 2 binding By similarity
Regioni656 – 6572Substrate 2 binding By similarity
Regioni739 – 7424Substrate 2 binding By similarity
Regioni861 – 8633Glucose-6-phosphate 2 binding By similarity

Domaini

The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus.

Sequence similaritiesi

Belongs to the hexokinase family.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG5026.
GeneTreeiENSGT00390000017159.
HOGENOMiHOG000162671.
HOVERGENiHBG005020.
InParanoidiP27881.
KOiK00844.
OMAiHHLGLES.
OrthoDBiEOG7S21X5.
PhylomeDBiP27881.
TreeFamiTF314238.

Family and domain databases

InterProiIPR001312. Hexokinase.
IPR022673. Hexokinase_C.
IPR019807. Hexokinase_CS.
IPR022672. Hexokinase_N.
[Graphical view]
PANTHERiPTHR19443. PTHR19443. 1 hit.
PfamiPF00349. Hexokinase_1. 2 hits.
PF03727. Hexokinase_2. 2 hits.
[Graphical view]
PRINTSiPR00475. HEXOKINASE.
PROSITEiPS00378. HEXOKINASES. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P27881-1 [UniParc]FASTAAdd to Basket

« Hide

MIASHMIACL FTELNQNQVQ KVDQFLYHMR LSDETLLEIS RRFRKEMEKG    50
LGATTHPTAA VKMLPTFVRS TPDGTEHGEF LALDLGGTNF RVLRVRVTDN 100
GLQRVEMENQ IYAIPEDIMR GSGTQLFDHI AECLANFMDK LQIKEKKLPL 150
GFTFSFPCHQ TKLDESFLVS WTKGFKSSGV EGRDVVDLIR KAIQRRGDFD 200
IDIVAVVNDT VGTMMTCGYD DQNCEIGLIV GTGSNACYME EMRHIDMVEG 250
DEGRMCINME WGAFGDDGTL NDIRTEFDRE IDMGSLNPGK QLFEKMISGM 300
YMGELVRLIL VKMAKAELLF QGKLSPELLT TGSFETKDVS DIEEDKDGIE 350
KAYQILMRLG LNPLQEDCVA THRICQIVST RSASLCAATL AAVLWRIKEN 400
KGEERLRSTI GVDGSVYKKH PHFAKRLHKA VRRLVPDCDV RFLRSEDGSG 450
KGAAMVTAVA YRLADQHRAR QKTLESLKLS HEQLLEVKRR MKVEMEQGLS 500
KETHAVAPVK MLPTYVCATP DGTEKGDFLA LDLGGTNFRV LLVRVRNGKR 550
RGVEMHNKIY SIPQEVMHGT GEELFDHIVQ CIADFLEYMG MKGVSLPLGF 600
TFSFPCQQNS LDQSILLKWT KGFKASGCEG EDVVTLLKEA IHRREEFDLD 650
VVAVVNDTVG TMMTCGYEDP HCEVGLIVGT GSNACYMEEM RNVELVDGEE 700
GRMCVNMEWG AFGDNGCLDD LRTVFDVAVD ELSLNPGKQR FEKMISGMYL 750
GEIVRNILID FTKRGLLFRG RISERLKTRG IFETKFLSQI ESDCLALLQV 800
RAILRHLGLE STCDDSIIVK EVCTVVARRA AQLCGAGMAA VVDKIRENRG 850
LDNLKVTVGV DGTLYKLHPH FAKVMHETVR DLAPKCDVSF LESEDGSGKG 900
AALITAVACR IREAGQR 917
Length:917
Mass (Da):102,544
Last modified:August 1, 1992 - v1
Checksum:i764EC189C0F90987
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M68971 mRNA. Translation: AAA41333.1.
M68972 mRNA. Translation: AAA41334.1.
D26393 Genomic DNA. Translation: BAA05409.1.
U19605 Genomic DNA. Translation: AAB09025.1.
AY082375 Genomic DNA. Translation: AAL92551.1.
PIRiS15885.
RefSeqiNP_036867.1. NM_012735.2.
UniGeneiRn.91375.

Genome annotation databases

EnsembliENSRNOT00000008813; ENSRNOP00000008813; ENSRNOG00000006116.
GeneIDi25059.
KEGGirno:25059.
UCSCiRGD:2797. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M68971 mRNA. Translation: AAA41333.1 .
M68972 mRNA. Translation: AAA41334.1 .
D26393 Genomic DNA. Translation: BAA05409.1 .
U19605 Genomic DNA. Translation: AAB09025.1 .
AY082375 Genomic DNA. Translation: AAL92551.1 .
PIRi S15885.
RefSeqi NP_036867.1. NM_012735.2.
UniGenei Rn.91375.

3D structure databases

ProteinModelPortali P27881.
SMRi P27881. Positions 17-913.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 247136. 3 interactions.
DIPi DIP-37314N.
IntActi P27881. 1 interaction.
MINTi MINT-1775746.
STRINGi 10116.ENSRNOP00000008813.

Chemistry

BindingDBi P27881.
ChEMBLi CHEMBL5063.

PTM databases

PhosphoSitei P27881.

Proteomic databases

PaxDbi P27881.
PRIDEi P27881.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000008813 ; ENSRNOP00000008813 ; ENSRNOG00000006116 .
GeneIDi 25059.
KEGGi rno:25059.
UCSCi RGD:2797. rat.

Organism-specific databases

CTDi 3099.
RGDi 2797. Hk2.

Phylogenomic databases

eggNOGi COG5026.
GeneTreei ENSGT00390000017159.
HOGENOMi HOG000162671.
HOVERGENi HBG005020.
InParanoidi P27881.
KOi K00844.
OMAi HHLGLES.
OrthoDBi EOG7S21X5.
PhylomeDBi P27881.
TreeFami TF314238.

Enzyme and pathway databases

UniPathwayi UPA00242 .
BRENDAi 2.7.1.1. 5301.
Reactomei REACT_205960. Glucose transport.
SABIO-RK P27881.

Miscellaneous databases

NextBioi 605276.
PROi P27881.

Gene expression databases

Genevestigatori P27881.

Family and domain databases

InterProi IPR001312. Hexokinase.
IPR022673. Hexokinase_C.
IPR019807. Hexokinase_CS.
IPR022672. Hexokinase_N.
[Graphical view ]
PANTHERi PTHR19443. PTHR19443. 1 hit.
Pfami PF00349. Hexokinase_1. 2 hits.
PF03727. Hexokinase_2. 2 hits.
[Graphical view ]
PRINTSi PR00475. HEXOKINASE.
PROSITEi PS00378. HEXOKINASES. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete amino acid sequence of the type II isozyme of rat hexokinase, deduced from the cloned cDNA: comparison with a hexokinase from novikoff ascites tumor."
    Thelen A.P., Wilson J.E.
    Arch. Biochem. Biophys. 286:645-651(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 11-18.
    Tissue: Skeletal muscle.
  2. "Nucleotide sequence of the 5'-flanking region of the rat type II hexokinase gene."
    Shinohara Y., Ichihara J., Kogure K., Terada H.
    Biochim. Biophys. Acta 1260:365-368(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-21.
    Strain: Wistar.
  3. "Glucose catabolism in cancer cells. Isolation, sequence, and activity of the promoter for type II hexokinase."
    Mathupala S.P., Rempel A., Pedersen P.L.
    J. Biol. Chem. 270:16918-16925(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-21.
    Strain: Sprague-Dawley.
    Tissue: Hepatoma.
  4. "Normal type II hexokinase promoter, first exon, and first intron from hepatocytes."
    Rempel A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-21.
    Strain: Sprague-Dawley.

Entry informationi

Entry nameiHXK2_RAT
AccessioniPrimary (citable) accession number: P27881
Secondary accession number(s): Q9QWR1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: September 3, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase).

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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