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Protein

Hexokinase-2

Gene

Hk2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + D-hexose = ADP + D-hexose 6-phosphate.

Enzyme regulationi

Hexokinase is an allosteric enzyme inhibited by its product Glc-6-P.

Pathwayi: hexose metabolism

This protein is involved in the pathway hexose metabolism, which is part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the pathway hexose metabolism and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei30ATP 1By similarity1
Binding sitei209Glucose-6-phosphate 1By similarity1
Binding sitei232Glucose-6-phosphate 1By similarity1
Binding sitei235Substrate 1By similarity1
Binding sitei260Substrate 1By similarity1
Binding sitei449Glucose-6-phosphate 1By similarity1
Binding sitei558ATP 2Sequence analysis1
Binding sitei657Glucose-6-phosphate 2By similarity1
Binding sitei680ATP 2By similarity1
Binding sitei680Glucose-6-phosphate 2By similarity1
Binding sitei683Substrate 2By similarity1
Binding sitei708Substrate 2By similarity1
Binding sitei897Glucose-6-phosphate 2By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi84 – 89ATP 1Sequence analysis6
Nucleotide bindingi425 – 426ATP 1By similarity2
Nucleotide bindingi532 – 537ATP 2By similarity6
Nucleotide bindingi747 – 748ATP 2By similarity2
Nucleotide bindingi784 – 788ATP 2By similarity5
Nucleotide bindingi863 – 867ATP 2By similarity5

GO - Molecular functioni

  • ATP binding Source: RGD
  • fructokinase activity Source: GO_Central
  • glucokinase activity Source: GO_Central
  • glucose binding Source: RGD
  • hexokinase activity Source: RGD
  • mannokinase activity Source: GO_Central

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.1. 5301.
ReactomeiR-RNO-70153. Glucose transport.
R-RNO-70171. Glycolysis.
SABIO-RKP27881.
UniPathwayiUPA00242.

Names & Taxonomyi

Protein namesi
Recommended name:
Hexokinase-2 (EC:2.7.1.1)
Alternative name(s):
Hexokinase type II
Short name:
HK II
Gene namesi
Name:Hk2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi2797. Hk2.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: GO_Central
  • mitochondrial outer membrane Source: RGD
  • mitochondrion Source: RGD
Complete GO annotation...

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL5063.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001975891 – 917Hexokinase-2Add BLAST917

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP27881.
PRIDEiP27881.

PTM databases

iPTMnetiP27881.
PhosphoSitePlusiP27881.

Expressioni

Gene expression databases

BgeeiENSRNOG00000006116.
GenevisibleiP27881. RN.

Interactioni

Subunit structurei

Monomer. Interacts with TIGAR; the interaction increases hexokinase HK2 activity in a hypoxia- and HIF1A-dependent manner.By similarity

Protein-protein interaction databases

BioGridi247136. 3 interactors.
DIPiDIP-37314N.
IntActiP27881. 1 interactor.
MINTiMINT-1775746.
STRINGi10116.ENSRNOP00000008813.

Chemistry databases

BindingDBiP27881.

Structurei

3D structure databases

ProteinModelPortaliP27881.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini16 – 458Hexokinase 1PROSITE-ProRule annotationAdd BLAST443
Domaini464 – 906Hexokinase 2PROSITE-ProRule annotationAdd BLAST443

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 12HydrophobicAdd BLAST12
Regioni13 – 475RegulatoryAdd BLAST463
Regioni73 – 207Hexokinase small subdomain 1PROSITE-ProRule annotationAdd BLAST135
Regioni84 – 88Glucose-6-phosphate 1 bindingBy similarity5
Regioni155 – 156Substrate 1 bindingBy similarity2
Regioni172 – 173Substrate 1 bindingBy similarity2
Regioni208 – 447Hexokinase large subdomain 1PROSITE-ProRule annotationAdd BLAST240
Regioni208 – 209Substrate 1 bindingBy similarity2
Regioni291 – 294Substrate 1 bindingBy similarity4
Regioni413 – 415Glucose-6-phosphate 1 bindingBy similarity3
Regioni476 – 917CatalyticAdd BLAST442
Regioni521 – 655Hexokinase small subdomain 2PROSITE-ProRule annotationAdd BLAST135
Regioni532 – 536Glucose-6-phosphate 2 bindingBy similarity5
Regioni603 – 604Substrate 2 bindingBy similarity2
Regioni620 – 621Substrate 2 bindingBy similarity2
Regioni656 – 895Hexokinase large subdomain 2PROSITE-ProRule annotationAdd BLAST240
Regioni656 – 657Substrate 2 bindingBy similarity2
Regioni739 – 742Substrate 2 bindingBy similarity4
Regioni861 – 863Glucose-6-phosphate 2 bindingBy similarity3

Domaini

The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus.

Sequence similaritiesi

Belongs to the hexokinase family.PROSITE-ProRule annotationCurated
Contains 2 hexokinase domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1369. Eukaryota.
COG5026. LUCA.
GeneTreeiENSGT00390000017159.
HOGENOMiHOG000162671.
HOVERGENiHBG005020.
InParanoidiP27881.
KOiK00844.
OMAiHHLGLES.
OrthoDBiEOG091G08MD.
PhylomeDBiP27881.
TreeFamiTF314238.

Family and domain databases

InterProiIPR001312. Hexokinase.
IPR019807. Hexokinase_BS.
IPR022673. Hexokinase_C.
IPR022672. Hexokinase_N.
[Graphical view]
PANTHERiPTHR19443. PTHR19443. 1 hit.
PfamiPF00349. Hexokinase_1. 2 hits.
PF03727. Hexokinase_2. 2 hits.
[Graphical view]
PROSITEiPS00378. HEXOKINASE_1. 2 hits.
PS51748. HEXOKINASE_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P27881-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIASHMIACL FTELNQNQVQ KVDQFLYHMR LSDETLLEIS RRFRKEMEKG
60 70 80 90 100
LGATTHPTAA VKMLPTFVRS TPDGTEHGEF LALDLGGTNF RVLRVRVTDN
110 120 130 140 150
GLQRVEMENQ IYAIPEDIMR GSGTQLFDHI AECLANFMDK LQIKEKKLPL
160 170 180 190 200
GFTFSFPCHQ TKLDESFLVS WTKGFKSSGV EGRDVVDLIR KAIQRRGDFD
210 220 230 240 250
IDIVAVVNDT VGTMMTCGYD DQNCEIGLIV GTGSNACYME EMRHIDMVEG
260 270 280 290 300
DEGRMCINME WGAFGDDGTL NDIRTEFDRE IDMGSLNPGK QLFEKMISGM
310 320 330 340 350
YMGELVRLIL VKMAKAELLF QGKLSPELLT TGSFETKDVS DIEEDKDGIE
360 370 380 390 400
KAYQILMRLG LNPLQEDCVA THRICQIVST RSASLCAATL AAVLWRIKEN
410 420 430 440 450
KGEERLRSTI GVDGSVYKKH PHFAKRLHKA VRRLVPDCDV RFLRSEDGSG
460 470 480 490 500
KGAAMVTAVA YRLADQHRAR QKTLESLKLS HEQLLEVKRR MKVEMEQGLS
510 520 530 540 550
KETHAVAPVK MLPTYVCATP DGTEKGDFLA LDLGGTNFRV LLVRVRNGKR
560 570 580 590 600
RGVEMHNKIY SIPQEVMHGT GEELFDHIVQ CIADFLEYMG MKGVSLPLGF
610 620 630 640 650
TFSFPCQQNS LDQSILLKWT KGFKASGCEG EDVVTLLKEA IHRREEFDLD
660 670 680 690 700
VVAVVNDTVG TMMTCGYEDP HCEVGLIVGT GSNACYMEEM RNVELVDGEE
710 720 730 740 750
GRMCVNMEWG AFGDNGCLDD LRTVFDVAVD ELSLNPGKQR FEKMISGMYL
760 770 780 790 800
GEIVRNILID FTKRGLLFRG RISERLKTRG IFETKFLSQI ESDCLALLQV
810 820 830 840 850
RAILRHLGLE STCDDSIIVK EVCTVVARRA AQLCGAGMAA VVDKIRENRG
860 870 880 890 900
LDNLKVTVGV DGTLYKLHPH FAKVMHETVR DLAPKCDVSF LESEDGSGKG
910
AALITAVACR IREAGQR
Length:917
Mass (Da):102,544
Last modified:August 1, 1992 - v1
Checksum:i764EC189C0F90987
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M68971 mRNA. Translation: AAA41333.1.
M68972 mRNA. Translation: AAA41334.1.
D26393 Genomic DNA. Translation: BAA05409.1.
U19605 Genomic DNA. Translation: AAB09025.1.
AY082375 Genomic DNA. Translation: AAL92551.1.
PIRiS15885.
RefSeqiNP_036867.1. NM_012735.2.
UniGeneiRn.91375.

Genome annotation databases

EnsembliENSRNOT00000008813; ENSRNOP00000008813; ENSRNOG00000006116.
GeneIDi25059.
KEGGirno:25059.
UCSCiRGD:2797. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M68971 mRNA. Translation: AAA41333.1.
M68972 mRNA. Translation: AAA41334.1.
D26393 Genomic DNA. Translation: BAA05409.1.
U19605 Genomic DNA. Translation: AAB09025.1.
AY082375 Genomic DNA. Translation: AAL92551.1.
PIRiS15885.
RefSeqiNP_036867.1. NM_012735.2.
UniGeneiRn.91375.

3D structure databases

ProteinModelPortaliP27881.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi247136. 3 interactors.
DIPiDIP-37314N.
IntActiP27881. 1 interactor.
MINTiMINT-1775746.
STRINGi10116.ENSRNOP00000008813.

Chemistry databases

BindingDBiP27881.
ChEMBLiCHEMBL5063.

PTM databases

iPTMnetiP27881.
PhosphoSitePlusiP27881.

Proteomic databases

PaxDbiP27881.
PRIDEiP27881.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000008813; ENSRNOP00000008813; ENSRNOG00000006116.
GeneIDi25059.
KEGGirno:25059.
UCSCiRGD:2797. rat.

Organism-specific databases

CTDi3099.
RGDi2797. Hk2.

Phylogenomic databases

eggNOGiKOG1369. Eukaryota.
COG5026. LUCA.
GeneTreeiENSGT00390000017159.
HOGENOMiHOG000162671.
HOVERGENiHBG005020.
InParanoidiP27881.
KOiK00844.
OMAiHHLGLES.
OrthoDBiEOG091G08MD.
PhylomeDBiP27881.
TreeFamiTF314238.

Enzyme and pathway databases

UniPathwayiUPA00242.
BRENDAi2.7.1.1. 5301.
ReactomeiR-RNO-70153. Glucose transport.
R-RNO-70171. Glycolysis.
SABIO-RKP27881.

Miscellaneous databases

PROiP27881.

Gene expression databases

BgeeiENSRNOG00000006116.
GenevisibleiP27881. RN.

Family and domain databases

InterProiIPR001312. Hexokinase.
IPR019807. Hexokinase_BS.
IPR022673. Hexokinase_C.
IPR022672. Hexokinase_N.
[Graphical view]
PANTHERiPTHR19443. PTHR19443. 1 hit.
PfamiPF00349. Hexokinase_1. 2 hits.
PF03727. Hexokinase_2. 2 hits.
[Graphical view]
PROSITEiPS00378. HEXOKINASE_1. 2 hits.
PS51748. HEXOKINASE_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHXK2_RAT
AccessioniPrimary (citable) accession number: P27881
Secondary accession number(s): Q9QWR1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: November 30, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase).

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.