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P27881

- HXK2_RAT

UniProt

P27881 - HXK2_RAT

Protein

Hexokinase-2

Gene

Hk2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 Aug 1992)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + D-hexose = ADP + D-hexose 6-phosphate.

    Enzyme regulationi

    Hexokinase is an allosteric enzyme inhibited by its product Glc-6-P.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei30 – 301ATP 1By similarity
    Binding sitei209 – 2091Glucose-6-phosphate 1By similarity
    Binding sitei232 – 2321Glucose-6-phosphate 1By similarity
    Binding sitei235 – 2351Substrate 1By similarity
    Binding sitei260 – 2601Substrate 1By similarity
    Binding sitei449 – 4491Glucose-6-phosphate 1By similarity
    Binding sitei558 – 5581ATP 2Sequence Analysis
    Binding sitei657 – 6571Glucose-6-phosphate 2By similarity
    Binding sitei680 – 6801ATP 2By similarity
    Binding sitei680 – 6801Glucose-6-phosphate 2By similarity
    Binding sitei683 – 6831Substrate 2By similarity
    Binding sitei708 – 7081Substrate 2By similarity
    Binding sitei897 – 8971Glucose-6-phosphate 2By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi84 – 896ATP 1Sequence Analysis
    Nucleotide bindingi425 – 4262ATP 1By similarity
    Nucleotide bindingi532 – 5376ATP 2By similarity
    Nucleotide bindingi747 – 7482ATP 2By similarity
    Nucleotide bindingi784 – 7885ATP 2By similarity
    Nucleotide bindingi863 – 8675ATP 2By similarity

    GO - Molecular functioni

    1. ATP binding Source: RGD
    2. fructokinase activity Source: RefGenome
    3. glucokinase activity Source: RefGenome
    4. glucose binding Source: RGD
    5. hexokinase activity Source: RGD
    6. mannokinase activity Source: RefGenome

    GO - Biological processi

    1. apoptotic mitochondrial changes Source: Ensembl
    2. carbohydrate phosphorylation Source: RGD
    3. cellular glucose homeostasis Source: RefGenome
    4. glucose 6-phosphate metabolic process Source: GOC
    5. glucose catabolic process Source: RGD
    6. glycolytic process Source: RefGenome
    7. lactation Source: RGD
    8. regulation of glucose import Source: Ensembl
    9. response to ischemia Source: RGD

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.1.1. 5301.
    ReactomeiREACT_205960. Glucose transport.
    SABIO-RKP27881.
    UniPathwayiUPA00242.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hexokinase-2 (EC:2.7.1.1)
    Alternative name(s):
    Hexokinase type II
    Short name:
    HK II
    Gene namesi
    Name:Hk2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 4

    Organism-specific databases

    RGDi2797. Hk2.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: RefGenome
    2. mitochondrial outer membrane Source: RGD
    3. mitochondrion Source: RGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 917917Hexokinase-2PRO_0000197589Add
    BLAST

    Proteomic databases

    PaxDbiP27881.
    PRIDEiP27881.

    PTM databases

    PhosphoSiteiP27881.

    Expressioni

    Gene expression databases

    GenevestigatoriP27881.

    Interactioni

    Subunit structurei

    Monomer.

    Protein-protein interaction databases

    BioGridi247136. 3 interactions.
    DIPiDIP-37314N.
    IntActiP27881. 1 interaction.
    MINTiMINT-1775746.
    STRINGi10116.ENSRNOP00000008813.

    Structurei

    3D structure databases

    ProteinModelPortaliP27881.
    SMRiP27881. Positions 17-913.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini17 – 221205Hexokinase type-1 1Add
    BLAST
    Domaini223 – 462240Hexokinase type-2 1Add
    BLAST
    Domaini465 – 669205Hexokinase type-1 2Add
    BLAST
    Domaini671 – 910240Hexokinase type-2 2Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 1212HydrophobicAdd
    BLAST
    Regioni13 – 475463RegulatoryAdd
    BLAST
    Regioni84 – 885Glucose-6-phosphate 1 bindingBy similarity
    Regioni155 – 1562Substrate 1 bindingBy similarity
    Regioni172 – 1732Substrate 1 bindingBy similarity
    Regioni208 – 2092Substrate 1 bindingBy similarity
    Regioni291 – 2944Substrate 1 bindingBy similarity
    Regioni413 – 4153Glucose-6-phosphate 1 bindingBy similarity
    Regioni476 – 917442CatalyticAdd
    BLAST
    Regioni532 – 5365Glucose-6-phosphate 2 bindingBy similarity
    Regioni603 – 6042Substrate 2 bindingBy similarity
    Regioni620 – 6212Substrate 2 bindingBy similarity
    Regioni656 – 6572Substrate 2 bindingBy similarity
    Regioni739 – 7424Substrate 2 bindingBy similarity
    Regioni861 – 8633Glucose-6-phosphate 2 bindingBy similarity

    Domaini

    The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus.

    Sequence similaritiesi

    Belongs to the hexokinase family.Curated
    Contains 2 hexokinase type-1 domains.Curated
    Contains 2 hexokinase type-2 domains.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG5026.
    GeneTreeiENSGT00390000017159.
    HOGENOMiHOG000162671.
    HOVERGENiHBG005020.
    InParanoidiP27881.
    KOiK00844.
    OMAiHHLGLES.
    OrthoDBiEOG7S21X5.
    PhylomeDBiP27881.
    TreeFamiTF314238.

    Family and domain databases

    InterProiIPR001312. Hexokinase.
    IPR022673. Hexokinase_C.
    IPR019807. Hexokinase_CS.
    IPR022672. Hexokinase_N.
    [Graphical view]
    PANTHERiPTHR19443. PTHR19443. 1 hit.
    PfamiPF00349. Hexokinase_1. 2 hits.
    PF03727. Hexokinase_2. 2 hits.
    [Graphical view]
    PRINTSiPR00475. HEXOKINASE.
    PROSITEiPS00378. HEXOKINASES. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P27881-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIASHMIACL FTELNQNQVQ KVDQFLYHMR LSDETLLEIS RRFRKEMEKG    50
    LGATTHPTAA VKMLPTFVRS TPDGTEHGEF LALDLGGTNF RVLRVRVTDN 100
    GLQRVEMENQ IYAIPEDIMR GSGTQLFDHI AECLANFMDK LQIKEKKLPL 150
    GFTFSFPCHQ TKLDESFLVS WTKGFKSSGV EGRDVVDLIR KAIQRRGDFD 200
    IDIVAVVNDT VGTMMTCGYD DQNCEIGLIV GTGSNACYME EMRHIDMVEG 250
    DEGRMCINME WGAFGDDGTL NDIRTEFDRE IDMGSLNPGK QLFEKMISGM 300
    YMGELVRLIL VKMAKAELLF QGKLSPELLT TGSFETKDVS DIEEDKDGIE 350
    KAYQILMRLG LNPLQEDCVA THRICQIVST RSASLCAATL AAVLWRIKEN 400
    KGEERLRSTI GVDGSVYKKH PHFAKRLHKA VRRLVPDCDV RFLRSEDGSG 450
    KGAAMVTAVA YRLADQHRAR QKTLESLKLS HEQLLEVKRR MKVEMEQGLS 500
    KETHAVAPVK MLPTYVCATP DGTEKGDFLA LDLGGTNFRV LLVRVRNGKR 550
    RGVEMHNKIY SIPQEVMHGT GEELFDHIVQ CIADFLEYMG MKGVSLPLGF 600
    TFSFPCQQNS LDQSILLKWT KGFKASGCEG EDVVTLLKEA IHRREEFDLD 650
    VVAVVNDTVG TMMTCGYEDP HCEVGLIVGT GSNACYMEEM RNVELVDGEE 700
    GRMCVNMEWG AFGDNGCLDD LRTVFDVAVD ELSLNPGKQR FEKMISGMYL 750
    GEIVRNILID FTKRGLLFRG RISERLKTRG IFETKFLSQI ESDCLALLQV 800
    RAILRHLGLE STCDDSIIVK EVCTVVARRA AQLCGAGMAA VVDKIRENRG 850
    LDNLKVTVGV DGTLYKLHPH FAKVMHETVR DLAPKCDVSF LESEDGSGKG 900
    AALITAVACR IREAGQR 917
    Length:917
    Mass (Da):102,544
    Last modified:August 1, 1992 - v1
    Checksum:i764EC189C0F90987
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M68971 mRNA. Translation: AAA41333.1.
    M68972 mRNA. Translation: AAA41334.1.
    D26393 Genomic DNA. Translation: BAA05409.1.
    U19605 Genomic DNA. Translation: AAB09025.1.
    AY082375 Genomic DNA. Translation: AAL92551.1.
    PIRiS15885.
    RefSeqiNP_036867.1. NM_012735.2.
    UniGeneiRn.91375.

    Genome annotation databases

    EnsembliENSRNOT00000008813; ENSRNOP00000008813; ENSRNOG00000006116.
    GeneIDi25059.
    KEGGirno:25059.
    UCSCiRGD:2797. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M68971 mRNA. Translation: AAA41333.1 .
    M68972 mRNA. Translation: AAA41334.1 .
    D26393 Genomic DNA. Translation: BAA05409.1 .
    U19605 Genomic DNA. Translation: AAB09025.1 .
    AY082375 Genomic DNA. Translation: AAL92551.1 .
    PIRi S15885.
    RefSeqi NP_036867.1. NM_012735.2.
    UniGenei Rn.91375.

    3D structure databases

    ProteinModelPortali P27881.
    SMRi P27881. Positions 17-913.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 247136. 3 interactions.
    DIPi DIP-37314N.
    IntActi P27881. 1 interaction.
    MINTi MINT-1775746.
    STRINGi 10116.ENSRNOP00000008813.

    Chemistry

    BindingDBi P27881.
    ChEMBLi CHEMBL5063.

    PTM databases

    PhosphoSitei P27881.

    Proteomic databases

    PaxDbi P27881.
    PRIDEi P27881.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000008813 ; ENSRNOP00000008813 ; ENSRNOG00000006116 .
    GeneIDi 25059.
    KEGGi rno:25059.
    UCSCi RGD:2797. rat.

    Organism-specific databases

    CTDi 3099.
    RGDi 2797. Hk2.

    Phylogenomic databases

    eggNOGi COG5026.
    GeneTreei ENSGT00390000017159.
    HOGENOMi HOG000162671.
    HOVERGENi HBG005020.
    InParanoidi P27881.
    KOi K00844.
    OMAi HHLGLES.
    OrthoDBi EOG7S21X5.
    PhylomeDBi P27881.
    TreeFami TF314238.

    Enzyme and pathway databases

    UniPathwayi UPA00242 .
    BRENDAi 2.7.1.1. 5301.
    Reactomei REACT_205960. Glucose transport.
    SABIO-RK P27881.

    Miscellaneous databases

    NextBioi 605276.
    PROi P27881.

    Gene expression databases

    Genevestigatori P27881.

    Family and domain databases

    InterProi IPR001312. Hexokinase.
    IPR022673. Hexokinase_C.
    IPR019807. Hexokinase_CS.
    IPR022672. Hexokinase_N.
    [Graphical view ]
    PANTHERi PTHR19443. PTHR19443. 1 hit.
    Pfami PF00349. Hexokinase_1. 2 hits.
    PF03727. Hexokinase_2. 2 hits.
    [Graphical view ]
    PRINTSi PR00475. HEXOKINASE.
    PROSITEi PS00378. HEXOKINASES. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete amino acid sequence of the type II isozyme of rat hexokinase, deduced from the cloned cDNA: comparison with a hexokinase from novikoff ascites tumor."
      Thelen A.P., Wilson J.E.
      Arch. Biochem. Biophys. 286:645-651(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 11-18.
      Tissue: Skeletal muscle.
    2. "Nucleotide sequence of the 5'-flanking region of the rat type II hexokinase gene."
      Shinohara Y., Ichihara J., Kogure K., Terada H.
      Biochim. Biophys. Acta 1260:365-368(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-21.
      Strain: Wistar.
    3. "Glucose catabolism in cancer cells. Isolation, sequence, and activity of the promoter for type II hexokinase."
      Mathupala S.P., Rempel A., Pedersen P.L.
      J. Biol. Chem. 270:16918-16925(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-21.
      Strain: Sprague-Dawley.
      Tissue: Hepatoma.
    4. "Normal type II hexokinase promoter, first exon, and first intron from hepatocytes."
      Rempel A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-21.
      Strain: Sprague-Dawley.

    Entry informationi

    Entry nameiHXK2_RAT
    AccessioniPrimary (citable) accession number: P27881
    Secondary accession number(s): Q9QWR1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: August 1, 1992
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase).

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3