Reviewed, UniProtKB/Swiss-Prot P27867 (DHSO_RAT)

Names and origin

Protein names

Recommended name:
    Sorbitol dehydrogenase
    EC=1.1.1.14
Alternative name(s):
    L-iditol 2-dehydrogenase

Gene names

Name:	Sord
Synonyms:	Sdh1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	357 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	L-iditol + NAD+ = L-sorbose + NADH.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homotetramer.
Sequence similarities	Belongs to the zinc-containing alcohol dehydrogenase family.
Caution	Ref.2 reports a cDNA predicted to encode a protein with an extended N-terminus but there is no further evidence for the existence of such a protein.

Ontologies

Keywords
Ligand	Metal-binding NAD Zinc
Molecular function	Oxidoreductase
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to cadmium ion Inferred from direct assay. Source: RGD response to copper ion Inferred from direct assay. Source: RGD response to drug Inferred from expression pattern. Source: RGD response to hormone stimulus Inferred from direct assay. Source: RGD response to nutrient levels Inferred from expression pattern. Source: RGD response to osmotic stress Inferred from expression pattern. Source: RGD
Molecular function	L-iditol 2-dehydrogenase activity Inferred from electronic annotation. Source: EC identical protein binding Inferred from direct assay. Source: RGD zinc ion binding Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 357	356	Sorbitol dehydrogenase		PRO_0000000884
Sites
	Metal binding	45	1	Zinc; catalytic By similarity
	Metal binding	70	1	Zinc; catalytic By similarity
	Metal binding	71	1	Zinc; catalytic By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	169	1	Phosphoserine Ref.4
Experimental info
	Sequence conflict	259	1	T → D in CAA41761. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P27867-1 [UniParc]. Last modified July 10, 2007. Version 4. Checksum: E6F535775EF73D36	FASTA	357	38,235
	10 20 30 40 50 60 MAAPAKGENL SLVVHGPGDI RLENYPIPEL GPNDVLLKMH SVGICGSDVH YWEHGRIGDF 70 80 90 100 110 120 VVKKPMVLGH EAAGTVTKVG PMVKHLKPGD RVAIEPGVPR EIDEFCKIGR YNLTPSIFFC 130 140 150 160 170 180 ATPPDDGNLC RFYKHSADFC YKLPDSVTFE EGALIEPLSV GIYACRRGSV SLGNKVLVCG 190 200 210 220 230 240 AGPIGIVTLL VAKAMGASQV VVIDLSASRL AKAKEVGADF TIQVAKETPH DIAKKVESVL 250 260 270 280 290 300 GSKPEVTIEC TGAESSVQTG IYATHSGGTL VVVGMGPEMI NLPLVHAAVR EVDIKGVFRY 310 320 330 340 350 CNTWPMAVSM LASKTLNVKP LVTHRFPLEK AVEAFETAKK GLGLKVMIKC DPNDQNP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sorbitol dehydrogenase: cDNA coding for the rat enzyme. Variations within the alcohol dehydrogenase family independent of quaternary structure and metal content." Karlsson C., Joernvall H., Heoeog J.O. Eur. J. Biochem. 198:761-765(1991) [PubMed: 2050152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"Sorbitol dehydrogenase. Full-length cDNA sequencing reveals a mRNA coding for a protein containing an additional 42 amino acids at the N-terminal end." Wen Y., Bekhor I. Eur. J. Biochem. 217:83-87(1993) [PubMed: 8223590] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Testis.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryonic liver, Liver and Testis.
[4]	"Phosphoproteomic analysis of rat liver by high capacity IMAC and LC-MS/MS." Moser K., White F.M. J. Proteome Res. 5:98-104(2006) [PubMed: 16396499] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-169, MASS SPECTROMETRY. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X59037 mRNA. Translation: CAA41761.1. X74593 mRNA. Translation: CAA52670.1. Different initiation. BC088398 mRNA. Translation: AAH88398.2. BC098919 mRNA. Translation: AAH98919.2. BC128707 mRNA. Translation: AAI28708.2.
IPI	IPI00760137.
PIR	S16132. S38363.
RefSeq	NP_058748.2.
UniGene	Rn.11334
3D structure databases
HSSP	HSSP built from PDB template 1E3J based on UniProtKB O96496.
SMR	P27867. Positions 44-399.
ModBase	Search...
PTM databases
PhosphoSite	P27867.
Proteomic databases
PRIDE	P27867.
Genome annotation databases
Ensembl	ENSRNOG00000017291. Rattus norvegicus. [Contig view]
GeneID	24788.
KEGG	rno:24788.
NMPDR	fig|10116.3.peg.19584.
Organism-specific databases
RGD	3734. Sord.
Phylogenomic databases
HOVERGEN	P27867.
Enzyme and pathway databases
BRENDA	1.1.1.14. 248.
Gene expression databases
GermOnline	ENSRNOG00000017291. Rattus norvegicus.
Family and domain databases
InterPro	IPR013154. ADH_GroES-like. IPR002085. ADH_SF_Zn. IPR013149. ADH_Zn-bd. IPR002328. ADH_Zn_CS. [Graphical view]
PANTHER	PTHR11695. ADH_Sf_Zn. 1 hit.
Pfam	PF08240. ADH_N. 1 hit. PF00107. ADH_zinc_N. 1 hit. [Graphical view]
ProDom	PD040557. GroES_related. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00059. ADH_ZINC. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	604416.

Entry information

Entry name

DHSO_RAT

Accession

Primary (citable) accession number: P27867
Secondary accession number(s): A2VCV9, Q4FZY4, Q5I0F3

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	July 10, 2007
Last modified:	June 16, 2009
This is version 95 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)