Reviewed, UniProtKB/Swiss-Prot P27859 (TATD_ECOLI)

Names and origin

Protein names

Recommended name:
    Deoxyribonuclease tatD
      Short name=DNase tatD
    EC=3.1.21.-

Gene names

Name:	tatD
Synonyms:	mttC, yigW, yigX
Ordered Locus Names:	b4483, JW5931

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	260 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Shows DNase activity.
Cofactor	Magnesium.
Subunit structure	Monomer Probable.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the tatD DNase family.
Caution	Was originally (Ref.2) thought to participate in the sec-independent protein translocase system.
Sequence caution	The sequence AAA67636.1 differs from that shown. Reason: Frameshift at position 160. The sequence AAA67637.1 differs from that shown. Reason: Frameshift at position 182.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Magnesium
Molecular function	Hydrolase Nuclease
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	endodeoxyribonuclease activity, producing 5'-phosphomonoesters Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 260

260

Deoxyribonuclease tatD

PRO_0000201992

Secondary structure

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260

Helix Strand Turn

Details...

Beta strand

2 – 6

5

Helix

11 – 13

3

Turn

14 – 16

3

Helix

17 – 26

10

Beta strand

31 – 34

4

Helix

39 – 51

13

Beta strand

55 – 59

5

Helix

63 – 68

6

Helix

71 – 81

11

Beta strand

86 – 95

10

Turn

96 – 98

3

Helix

103 – 120

18

Beta strand

124 – 130

7

Helix

132 – 139

8

Helix

140 – 145

6

Beta strand

149 – 151

3

Helix

158 – 166

9

Beta strand

170 – 173

4

Helix

175 – 178

4

Turn

180 – 182

3

Helix

184 – 189

6

Helix

190 – 192

3

Helix

195 – 197

3

Beta strand

198 – 200

3

Helix

223 – 225

3

Helix

226 – 236

11

Helix

241 – 256

16

Sequences

	Sequence		Length	Mass (Da)
	P27859-1 [UniParc]. Last modified December 1, 2000. Version 3. Checksum: C18FB5269849746F	FASTA	260	28,974
	10 20 30 40 50 60 MFDIGVNLTS SQFAKDRDDV VACAFDAGVN GLLITGTNLR ESQQAQKLAR QYSSCWSTAG 70 80 90 100 110 120 VHPHDSSQWQ AATEEAIIEL AAQPEVVAIG ECGLDFNRNF STPEEQERAF VAQLRIAADL 130 140 150 160 170 180 NMPVFMHCRD AHERFMTLLE PWLDKLPGAV LHCFTGTREE MQACVAHGIY IGITGWVCDE 190 200 210 220 230 240 RRGLELRELL PLIPAEKLLI ETDAPYLLPR DLTPKPSSRR NEPAHLPHIL QRIAHWRGED 250 260 AAWLAATTDA NVKTLFGIAF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Escherichia coli HlyT protein, a transcriptional activator of haemolysin synthesis and secretion, is encoded by the rfaH (sfrB) locus required for expression of sex factor and lipopolysaccharide genes." Bailey M.J.A., Koronakis V., Schmoll T., Hughes C. Mol. Microbiol. 6:1003-1012(1992) [PubMed: 1584020] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 5KC.
[2]	"Overlapping functions of components of a bacterial Sec-independent protein export pathway." Sargent F., Bogsch E.G., Stanley N.R., Wexler M., Robinson C., Berks B.C., Palmer T. EMBO J. 17:3640-3650(1998) [PubMed: 9649434] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], GENE NAME. Strain: K12.
[3]	"Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes." Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R. Science 257:771-778(1992) [PubMed: 1379743] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Escherichia coli K-12: a cooperatively developed annotation snapshot -- 2005." Riley M., Abe T., Arnaud M.B., Berlyn M.K.B., Blattner F.R., Chaudhuri R.R., Glasner J.D., Horiuchi T., Keseler I.M., Kosuge T., Mori H., Perna N.T., Plunkett G. III, Rudd K.E., Serres M.H., Thomas G.H., Thomson N.R., Wishart D., Wanner B.L. Nucleic Acids Res. 34:1-9(2006) [PubMed: 16397293] [Abstract] Cited for: SEQUENCE REVISION.
[6]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[7]	"TatD is a cytoplasmic protein with DNase activity. No requirement for TatD family proteins in sec-independent protein export." Wexler M., Sargent F., Jack R.L., Stanley N.R., Bogsch E.G., Robinson C., Berks B.C., Palmer T. J. Biol. Chem. 275:16717-16722(2000) [PubMed: 10747959] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE OF 1-6, CHARACTERIZATION.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X65013 Genomic DNA. No translation available.
AJ005830 Genomic DNA. Translation: CAA06727.1. Different initiation.
M87049 Genomic DNA. Translation: AAA67636.1. Frameshift.
M87049 Genomic DNA. Translation: AAA67637.1. Frameshift.
U00096 Genomic DNA. Translation: AAT48229.1.
AP009048 Genomic DNA. Translation: BAE77462.1.

PIR

A65189.

RefSeq

AP_003961.1.
YP_026271.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1XWY	X-ray	2.00	A	1-260	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:10961N.

STRING

P27859.

Genome annotation databases

GeneID

2847752.

GenomeReviews

Gene locus JW5931 in contig AP009048_GR.
Gene locus b4483 in contig U00096_GR.

KEGG

ecj:JW5931.
eco:b4483.

Organism-specific databases

EchoBASE

EB1446.

EcoGene

EG11481. tatD.

CMR

Search...

Phylogenomic databases

HOGENOM

P27859.

OMA

GTNLHES

Enzyme and pathway databases

BioCyc

EcoCyc:EG11481-MON.

Gene expression databases

Genevestigator

P27859.

Family and domain databases

InterPro

IPR015992. DNase_TatD.
IPR001130. DNase_TatD-rel.
IPR018228. DNase_TatD-rel_CS.
IPR012278. DNase_TatD_Mg.
[Graphical view]

PANTHER

PTHR10060. TatD_DNase. 1 hit.

Pfam

PF01026. TatD_DNase. 1 hit.
[Graphical view]

PIRSF

PIRSF005902. DNase_TatD. 1 hit.

PROSITE

PS01137. TATD_1. False negative.
PS01090. TATD_2. 1 hit.
PS01091. TATD_3. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

TATD_ECOLI

Accession

Primary (citable) accession number: P27859
Secondary accession number(s): P27860 , P78128, P78129, Q2M8E4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	December 1, 2000
Last modified:	November 24, 2009
This is version 87 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)