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Protein

Pyridoxal phosphate phosphatase YigL

Gene

yigL

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes Strongly the dephosphorylation of pyridoxal-phosphate (PLP) and moderately the dephosphorylation of 2-deoxyglucose 6-phosphate (2bGLU6P) and beta-glucose 6-phosphate (bGlu6P). Also hydrolyzes both purines (GMP and IMP) and pyrimidines as secondary substrates.1 Publication

Catalytic activityi

Pyridoxal 5'-phosphate + H2O = pyridoxal + phosphate.1 Publication
Sugar phosphate + H2O = sugar + phosphate.1 Publication

Cofactori

Mg2+1 Publication, Mn2+1 Publication, Co2+1 Publication, Zn2+1 PublicationNote: Magnesium. Can also use other divalent metal cations as manganese, cobalt or zinc.1 Publication

Kineticsi

  1. KM=1.5 mM for PLP (in the presence of magnesium ion as cofactor and at pH 9)1 Publication
  2. KM=5.9 mM for bGlu6P (in the presence of magnesium ion as cofactor and at pH 9)1 Publication
  3. KM=7.5 mM for 2bGLU6P (in the presence of magnesium ion as cofactor and at pH 9)1 Publication

    pH dependencei

    Optimum pH is between 6 and 7.5.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei8NucleophileBy similarity1
    Metal bindingi8MagnesiumBy similarity1
    Binding sitei9Phosphate; via amide nitrogenBy similarity1
    Metal bindingi10Magnesium; via carbonyl oxygenBy similarity1
    Binding sitei191PhosphateBy similarity1
    Metal bindingi214MagnesiumBy similarity1
    Binding sitei217PhosphateBy similarity1

    GO - Molecular functioni

    • magnesium ion binding Source: UniProtKB
    • metal ion binding Source: EcoliWiki
    • phosphatase activity Source: EcoliWiki
    • pyridoxal phosphatase activity Source: EcoliWiki
    • sugar-phosphatase activity Source: EcoliWiki

    Keywordsi

    Molecular functionHydrolase
    LigandMagnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:EG11470-MONOMER
    MetaCyc:EG11470-MONOMER

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyridoxal phosphate phosphatase YigL (EC:3.1.3.74)
    Alternative name(s):
    PLP phosphatase
    Sugar phosphatase (EC:3.1.3.23)
    Gene namesi
    Name:yigL
    Ordered Locus Names:b3826, JW5854
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG11470 yigL

    Subcellular locationi

    GO - Cellular componenti

    • cytosol Source: EcoCyc

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000544261 – 266Pyridoxal phosphate phosphatase YigLAdd BLAST266

    Proteomic databases

    EPDiP27848
    PaxDbiP27848
    PRIDEiP27848

    Interactioni

    Protein-protein interaction databases

    BioGridi425960911 interactors.
    IntActiP27848 2 interactors.
    STRINGi316385.ECDH10B_4017

    Structurei

    3D structure databases

    ProteinModelPortaliP27848
    SMRiP27848
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni42 – 43Phosphate bindingBy similarity2

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG4105DQF Bacteria
    COG0561 LUCA
    HOGENOMiHOG000184784
    InParanoidiP27848
    OMAiGKGCIMQ
    PhylomeDBiP27848

    Family and domain databases

    Gene3Di3.40.50.10002 hits
    InterProiView protein in InterPro
    IPR000150 Cof
    IPR036412 HAD-like_sf
    IPR006379 HAD-SF_hydro_IIB
    IPR023214 HAD_sf
    SUPFAMiSSF56784 SSF56784, 1 hit
    TIGRFAMsiTIGR00099 Cof-subfamily, 1 hit
    TIGR01484 HAD-SF-IIB, 1 hit
    PROSITEiView protein in PROSITE
    PS01228 COF_1, 1 hit
    PS01229 COF_2, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P27848-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MYQVVASDLD GTLLSPDHTL SPYAKETLKL LTARGINFVF ATGRHHVDVG
    60 70 80 90 100
    QIRDNLEIKS YMITSNGARV HDLDGNLIFA HNLDRDIASD LFGVVNDNPD
    110 120 130 140 150
    IITNVYRDDE WFMNRHRPEE MRFFKEAVFQ YALYEPGLLE PEGVSKVFFT
    160 170 180 190 200
    CDSHEQLLPL EQAINARWGD RVNVSFSTLT CLEVMAGGVS KGHALEAVAK
    210 220 230 240 250
    KLGYSLKDCI AFGDGMNDAE MLSMAGKGCI MGSAHQRLKD LHPELEVIGT
    260
    NADDAVPHYL RKLYLS
    Length:266
    Mass (Da):29,708
    Last modified:October 11, 2004 - v4
    Checksum:i7C9AFE9CF13D8B2B
    GO

    Sequence cautioni

    The sequence AAA67622 differs from that shown. Reason: Frameshift at several positions.Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M87049 Genomic DNA Translation: AAA67622.1 Frameshift.
    U00096 Genomic DNA Translation: AAT48225.1
    AP009048 Genomic DNA Translation: BAE77475.1
    X03155 Genomic DNA No translation available.
    RefSeqiWP_000285362.1, NZ_LN832404.1
    YP_026267.1, NC_000913.3

    Genome annotation databases

    EnsemblBacteriaiAAT48225; AAT48225; b3826
    BAE77475; BAE77475; BAE77475
    GeneIDi2847768
    KEGGiecj:JW5854
    eco:b3826
    PATRICifig|511145.12.peg.3942

    Similar proteinsi

    Entry informationi

    Entry nameiYIGL_ECOLI
    AccessioniPrimary (citable) accession number: P27848
    Secondary accession number(s): P76763, Q2M8D1, Q6BEY9
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: October 11, 2004
    Last modified: March 28, 2018
    This is version 133 of the entry and version 4 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome