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Protein

Iron-sulfur cluster assembly protein CyaY

Gene

cyaY

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in iron-sulfur (Fe-S) cluster assembly (PubMed:16603772, PubMed:19305405, PubMed:21799759, PubMed:25430730). May act as a regulator of Fe-S biogenesis (PubMed:19305405, PubMed:21799759, PubMed:25430730). Can bind both Fe2+ and Fe3+ ions (PubMed:15530368, PubMed:16603772, PubMed:17244611, PubMed:17651435). In vivo, has a positive effect on Fe-S cluster biogenesis under iron-rich growth conditions (PubMed:25430730). In vitro, can inhibit IscS cysteine desulfurase activity and the formation of Fe-S clusters on IscU (PubMed:19305405, PubMed:21799759). In vitro, in the presence of IscS and cysteine, Fe3+-CyaY can be used as an iron donor during Fe-S cluster assembly on the scaffold protein IscU (PubMed:16603772).7 Publications

Enzyme regulationi

Binds iron under oxidative stress conditions, but not under conditions emulating the intracellular redox potential.1 Publication

GO - Molecular functioni

  • 2 iron, 2 sulfur cluster binding Source: GO_Central
  • ferric iron binding Source: EcoCyc
  • ferrous iron binding Source: EcoCyc
  • ferroxidase activity Source: GO_Central
  • iron chaperone activity Source: GO_Central

GO - Biological processi

Keywordsi

LigandIron, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG11653-MONOMER
MetaCyc:EG11653-MONOMER

Protein family/group databases

TCDBi9.B.21.2.1 the frataxin (frataxin) family

Names & Taxonomyi

Protein namesi
Recommended name:
Iron-sulfur cluster assembly protein CyaYUniRule annotationCurated
Alternative name(s):
Bacterial frataxin ortholog1 Publication
Gene namesi
Name:cyaY1 PublicationUniRule annotation
Ordered Locus Names:b3807, JW3779
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11653 cyaY

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoliWiki
  • cytosol Source: EcoCyc

Pathology & Biotechi

Disruption phenotypei

Cells are not affected in cellular iron content and sensitivity to oxidants.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi18E → K: Abolishes iron binding and increases kinetics of Fe-S formation on IscU; when associated with K-19 and K-22. 2 Publications1
Mutagenesisi19E → K: Abolishes iron binding and increases kinetics of Fe-S formation on IscU; when associated with K-18 and K-22. 2 Publications1
Mutagenesisi22D → K: Abolishes iron binding and increases kinetics of Fe-S formation on IscU; when associated with K-18 and K-19. 2 Publications1
Mutagenesisi31D → K: Increases kinetics of Fe-S formation on IscU. 1 Publication1
Mutagenesisi61W → R: Increases kinetics of Fe-S formation on IscU. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001939361 – 106Iron-sulfur cluster assembly protein CyaYAdd BLAST106

Proteomic databases

PaxDbiP27838
PRIDEiP27838

Interactioni

Subunit structurei

Monomer (PubMed:16603772, PubMed:19305405, PubMed:28931050). Forms homodimers and higher oligomers in the presence of iron (PubMed:16603772, PubMed:28931050). Interacts with IscS (PubMed:16603772, PubMed:19305405, PubMed:25430730).4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
iscSP0A6B72EBI-9146621,EBI-550055

Protein-protein interaction databases

BioGridi4260671, 116 interactors
DIPiDIP-9356N
IntActiP27838, 3 interactors
STRINGi316385.ECDH10B_3998

Structurei

Secondary structure

1106
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 22Combined sources20
Beta strandi26 – 28Combined sources3
Beta strandi31 – 35Combined sources5
Beta strandi38 – 42Combined sources5
Beta strandi48 – 54Combined sources7
Turni55 – 58Combined sources4
Beta strandi59 – 63Combined sources5
Beta strandi68 – 74Combined sources7
Beta strandi77 – 80Combined sources4
Turni81 – 83Combined sources3
Helixi87 – 99Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EW4X-ray1.40A1-106[»]
1SOYNMR-A1-106[»]
2EFFX-ray1.80A1-106[»]
2P1XX-ray1.42A1-106[»]
ProteinModelPortaliP27838
SMRiP27838
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP27838

Family & Domainsi

Sequence similaritiesi

Belongs to the frataxin family.UniRule annotationCurated

Phylogenomic databases

eggNOGiENOG4105XQ6 Bacteria
COG1965 LUCA
HOGENOMiHOG000190728
InParanoidiP27838
KOiK06202
OMAiNRQEPMH
PhylomeDBiP27838

Family and domain databases

Gene3Di3.30.920.10, 1 hit
HAMAPiMF_00142 CyaY, 1 hit
InterProiView protein in InterPro
IPR002908 Frataxin/CyaY
IPR036524 Frataxin/CyaY_sf
IPR020895 Frataxin_CS
PANTHERiPTHR16821 PTHR16821, 1 hit
PfamiView protein in Pfam
PF01491 Frataxin_Cyay, 1 hit
SMARTiView protein in SMART
SM01219 Frataxin_Cyay, 1 hit
TIGRFAMsiTIGR03421 FeS_CyaY, 1 hit
PROSITEiView protein in PROSITE
PS01344 FRATAXIN_1, 1 hit
PS50810 FRATAXIN_2, 1 hit

Sequencei

Sequence statusi: Complete.

P27838-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNDSEFHRLA DQLWLTIEER LDDWDGDSDI DCEINGGVLT ITFENGSKII
60 70 80 90 100
INRQEPLHQV WLATKQGGYH FDLKGDEWIC DRSGETFWDL LEQAATQQAG

ETVSFR
Length:106
Mass (Da):12,231
Last modified:August 1, 1992 - v1
Checksum:i03EA6F330A48F711
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66782 Genomic DNA Translation: CAA47281.1
M87049 Genomic DNA Translation: AAA67603.1
U00096 Genomic DNA Translation: AAC76810.1
AP009048 Genomic DNA Translation: BAE77494.1
PIRiS30697
RefSeqiNP_418251.1, NC_000913.3
WP_000999947.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76810; AAC76810; b3807
BAE77494; BAE77494; BAE77494
GeneIDi947754
KEGGiecj:JW3779
eco:b3807
PATRICifig|1411691.4.peg.2901

Similar proteinsi

Entry informationi

Entry nameiCYAY_ECOLI
AccessioniPrimary (citable) accession number: P27838
Secondary accession number(s): Q2M8B2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: March 28, 2018
This is version 148 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health