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Protein

UDP-N-acetyl-D-mannosaminuronic acid transferase

Gene

wecG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the synthesis of Und-PP-GlcNAc-ManNAcA (Lipid II), the second lipid-linked intermediate involved in enterobacterial common antigen (ECA) synthesis.UniRule annotation1 Publication

Catalytic activityi

UDP-N-acetyl-alpha-D-mannosaminouronate + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol = UDP + N-acetyl-beta-D-mannosaminouronyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol.UniRule annotation1 Publication

Pathwayi: enterobacterial common antigen biosynthesis

This protein is involved in the pathway enterobacterial common antigen biosynthesis, which is part of Bacterial outer membrane biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway enterobacterial common antigen biosynthesis and in Bacterial outer membrane biogenesis.

GO - Molecular functioni

  • lipopolysaccharide N-acetylmannosaminouronosyltransferase activity Source: UniProtKB-EC
  • transferase activity, transferring hexosyl groups Source: EcoCyc

GO - Biological processi

  • enterobacterial common antigen biosynthetic process Source: EcoCyc

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciEcoCyc:UDPMANACATRANS-MONOMER
MetaCyc:UDPMANACATRANS-MONOMER
UniPathwayiUPA00566

Protein family/group databases

CAZyiGT26 Glycosyltransferase Family 26

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetyl-D-mannosaminuronic acid transferaseUniRule annotation (EC:2.4.1.180UniRule annotation)
Short name:
UDP-ManNAcA transferaseUniRule annotation
Gene namesi
Name:wecGUniRule annotation
Synonyms:rffMUniRule annotation
Ordered Locus Names:b3794, JW3770
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11458 rffM

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002084261 – 246UDP-N-acetyl-D-mannosaminuronic acid transferaseAdd BLAST246

Proteomic databases

PaxDbiP27836
PRIDEiP27836

Interactioni

Protein-protein interaction databases

BioGridi4261340, 190 interactors
DIPiDIP-11132N
IntActiP27836, 15 interactors
STRINGi316385.ECDH10B_3984

Structurei

3D structure databases

ProteinModelPortaliP27836
SMRiP27836
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 26 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108UHC Bacteria
COG1922 LUCA
HOGENOMiHOG000075380
InParanoidiP27836
KOiK02852
OMAiPWRWRRM
PhylomeDBiP27836

Family and domain databases

CDDicd06533 Glyco_transf_WecG_TagA, 1 hit
HAMAPiMF_01001 WecG_RffM, 1 hit
InterProiView protein in InterPro
IPR023085 UDP-ManNAcA_Trfase_WecG
IPR004629 WecB_TagA_CpsF
PANTHERiPTHR34136 PTHR34136, 1 hit
PfamiView protein in Pfam
PF03808 Glyco_tran_WecB, 1 hit
TIGRFAMsiTIGR00696 wecG_tagA_cpsF, 1 hit

Sequencei

Sequence statusi: Complete.

P27836-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNNTTAPTY TLRGLQLIGW RDMQHALDYL FADGQLKQGT LVAINAEKML
60 70 80 90 100
TIEDNAEVRE LINAAEFKYA DGISVVRSVR KKYPQAQVSR VAGADLWEEL
110 120 130 140 150
MARAGKEGTP VFLVGGKPEV LAQTEAKLRN QWNVNIVGSQ DGYFKPEQRQ
160 170 180 190 200
ALFERIHASG AQIVTVAMGS PKQEIIMRDC RLVHPDALYM GVGGTYDVFT
210 220 230 240
GHVKRAPKIW QTLGLEWLYR LLSQPSRIKR QLRLLRYLRW HYTGNL
Length:246
Mass (Da):27,928
Last modified:July 15, 1998 - v2
Checksum:i0D9435DEA6A69CAC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti120 – 121VL → AV in AAA67594 (PubMed:1379743).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M87049 Genomic DNA Translation: AAA67594.1
U00096 Genomic DNA Translation: AAC76801.1
AP009048 Genomic DNA Translation: BAE77503.1
PIRiG65183
RefSeqiNP_418242.1, NC_000913.3
WP_001064040.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76801; AAC76801; b3794
BAE77503; BAE77503; BAE77503
GeneIDi948301
KEGGiecj:JW3770
eco:b3794
PATRICifig|1411691.4.peg.2911

Entry informationi

Entry nameiWECG_ECOLI
AccessioniPrimary (citable) accession number: P27836
Secondary accession number(s): Q2M8A3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: July 15, 1998
Last modified: March 28, 2018
This is version 132 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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