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Protein

UDP-N-acetyl-D-mannosaminuronic acid transferase

Gene

wecG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the synthesis of Und-PP-GlcNAc-ManNAcA (Lipid II), the second lipid-linked intermediate involved in enterobacterial common antigen (ECA) synthesis.UniRule annotation1 Publication

Catalytic activityi

UDP-N-acetyl-alpha-D-mannosaminouronate + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol = UDP + N-acetyl-beta-D-mannosaminouronyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol.UniRule annotation1 Publication

Pathwayi: enterobacterial common antigen biosynthesis

This protein is involved in the pathway enterobacterial common antigen biosynthesis, which is part of Bacterial outer membrane biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway enterobacterial common antigen biosynthesis and in Bacterial outer membrane biogenesis.

GO - Molecular functioni

  • lipopolysaccharide N-acetylmannosaminouronosyltransferase activity Source: UniProtKB-HAMAP
  • transferase activity, transferring hexosyl groups Source: EcoCyc

GO - Biological processi

  • enterobacterial common antigen biosynthetic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciEcoCyc:UDPMANACATRANS-MONOMER.
ECOL316407:JW3770-MONOMER.
MetaCyc:UDPMANACATRANS-MONOMER.
UniPathwayiUPA00566.

Protein family/group databases

CAZyiGT26. Glycosyltransferase Family 26.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetyl-D-mannosaminuronic acid transferaseUniRule annotation (EC:2.4.1.180UniRule annotation)
Short name:
UDP-ManNAcA transferaseUniRule annotation
Gene namesi
Name:wecGUniRule annotation
Synonyms:rffMUniRule annotation
Ordered Locus Names:b3794, JW3770
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11458. rffM.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002084261 – 246UDP-N-acetyl-D-mannosaminuronic acid transferaseAdd BLAST246

Proteomic databases

PaxDbiP27836.
PRIDEiP27836.

Interactioni

Protein-protein interaction databases

BioGridi4261340. 190 interactors.
DIPiDIP-11132N.
IntActiP27836. 15 interactors.
MINTiMINT-1229961.
STRINGi511145.b3794.

Structurei

3D structure databases

ProteinModelPortaliP27836.
SMRiP27836.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 26 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108UHC. Bacteria.
COG1922. LUCA.
HOGENOMiHOG000075380.
InParanoidiP27836.
KOiK02852.
OMAiATPNAEI.
PhylomeDBiP27836.

Family and domain databases

CDDicd06533. Glyco_transf_WecG_TagA. 1 hit.
HAMAPiMF_01001. WecG_RffM. 1 hit.
InterProiIPR023085. UDP-ManNAcA_Trfase_WecG.
IPR004629. WecB_TagA_CpsF.
[Graphical view]
PfamiPF03808. Glyco_tran_WecB. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00696. wecG_tagA_cpsF. 1 hit.

Sequencei

Sequence statusi: Complete.

P27836-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNNTTAPTY TLRGLQLIGW RDMQHALDYL FADGQLKQGT LVAINAEKML
60 70 80 90 100
TIEDNAEVRE LINAAEFKYA DGISVVRSVR KKYPQAQVSR VAGADLWEEL
110 120 130 140 150
MARAGKEGTP VFLVGGKPEV LAQTEAKLRN QWNVNIVGSQ DGYFKPEQRQ
160 170 180 190 200
ALFERIHASG AQIVTVAMGS PKQEIIMRDC RLVHPDALYM GVGGTYDVFT
210 220 230 240
GHVKRAPKIW QTLGLEWLYR LLSQPSRIKR QLRLLRYLRW HYTGNL
Length:246
Mass (Da):27,928
Last modified:July 15, 1998 - v2
Checksum:i0D9435DEA6A69CAC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti120 – 121VL → AV in AAA67594 (PubMed:1379743).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M87049 Genomic DNA. Translation: AAA67594.1.
U00096 Genomic DNA. Translation: AAC76801.1.
AP009048 Genomic DNA. Translation: BAE77503.1.
PIRiG65183.
RefSeqiNP_418242.1. NC_000913.3.
WP_001064040.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76801; AAC76801; b3794.
BAE77503; BAE77503; BAE77503.
GeneIDi948301.
KEGGiecj:JW3770.
eco:b3794.
PATRICi32123083. VBIEscCol129921_3911.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M87049 Genomic DNA. Translation: AAA67594.1.
U00096 Genomic DNA. Translation: AAC76801.1.
AP009048 Genomic DNA. Translation: BAE77503.1.
PIRiG65183.
RefSeqiNP_418242.1. NC_000913.3.
WP_001064040.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP27836.
SMRiP27836.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261340. 190 interactors.
DIPiDIP-11132N.
IntActiP27836. 15 interactors.
MINTiMINT-1229961.
STRINGi511145.b3794.

Protein family/group databases

CAZyiGT26. Glycosyltransferase Family 26.

Proteomic databases

PaxDbiP27836.
PRIDEiP27836.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76801; AAC76801; b3794.
BAE77503; BAE77503; BAE77503.
GeneIDi948301.
KEGGiecj:JW3770.
eco:b3794.
PATRICi32123083. VBIEscCol129921_3911.

Organism-specific databases

EchoBASEiEB1427.
EcoGeneiEG11458. rffM.

Phylogenomic databases

eggNOGiENOG4108UHC. Bacteria.
COG1922. LUCA.
HOGENOMiHOG000075380.
InParanoidiP27836.
KOiK02852.
OMAiATPNAEI.
PhylomeDBiP27836.

Enzyme and pathway databases

UniPathwayiUPA00566.
BioCyciEcoCyc:UDPMANACATRANS-MONOMER.
ECOL316407:JW3770-MONOMER.
MetaCyc:UDPMANACATRANS-MONOMER.

Miscellaneous databases

PROiP27836.

Family and domain databases

CDDicd06533. Glyco_transf_WecG_TagA. 1 hit.
HAMAPiMF_01001. WecG_RffM. 1 hit.
InterProiIPR023085. UDP-ManNAcA_Trfase_WecG.
IPR004629. WecB_TagA_CpsF.
[Graphical view]
PfamiPF03808. Glyco_tran_WecB. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00696. wecG_tagA_cpsF. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiWECG_ECOLI
AccessioniPrimary (citable) accession number: P27836
Secondary accession number(s): Q2M8A3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: July 15, 1998
Last modified: November 2, 2016
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.