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Protein

Probable ECA polymerase

Gene

wzyE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Probably involved in the polymerization of enterobacterial common antigen (ECA) trisaccharide repeat units. Required for the assembly of the phosphoglyceride-linked form of ECA (ECA(PG)) and the water-soluble cyclic form of ECA (ECA(CYC)).1 Publication

Pathwayi: enterobacterial common antigen biosynthesis

This protein is involved in the pathway enterobacterial common antigen biosynthesis, which is part of Bacterial outer membrane biogenesis.UniRule annotation1 Publication
View all proteins of this organism that are known to be involved in the pathway enterobacterial common antigen biosynthesis and in Bacterial outer membrane biogenesis.

GO - Biological processi

  • enterobacterial common antigen biosynthetic process Source: EcoCyc
Complete GO annotation...

Enzyme and pathway databases

BioCyciEcoCyc:FUC4NACTRANS-MONOMER.
ECOL316407:JW3769-MONOMER.
MetaCyc:FUC4NACTRANS-MONOMER.
UniPathwayiUPA00566.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ECA polymeraseUniRule annotationCurated
Gene namesi
Name:wzyEUniRule annotation
Synonyms:wzy
Ordered Locus Names:b3793, JW3769
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11457. wzyE.

Subcellular locationi

  • Cell inner membrane UniRule annotationCurated; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei6 – 2621HelicalUniRule annotationAdd
BLAST
Transmembranei37 – 5721HelicalUniRule annotationAdd
BLAST
Transmembranei63 – 8321HelicalUniRule annotationAdd
BLAST
Transmembranei118 – 13821HelicalUniRule annotationAdd
BLAST
Transmembranei155 – 17521HelicalUniRule annotationAdd
BLAST
Transmembranei181 – 20121HelicalUniRule annotationAdd
BLAST
Transmembranei207 – 22721HelicalUniRule annotationAdd
BLAST
Transmembranei228 – 24821HelicalUniRule annotationAdd
BLAST
Transmembranei341 – 36121HelicalUniRule annotationAdd
BLAST
Transmembranei378 – 39821HelicalUniRule annotationAdd
BLAST
Transmembranei410 – 43021HelicalUniRule annotationAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: UniProtKB-HAMAP
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 450450Probable ECA polymerasePRO_0000208536Add
BLAST

Proteomic databases

PaxDbiP27835.

Interactioni

Subunit structurei

Probably part of a complex composed of WzxE, WzyE and WzzE.UniRule annotation1 Publication

Protein-protein interaction databases

BioGridi4263518. 285 interactions.
STRINGi511145.b3793.

Structurei

3D structure databases

ProteinModelPortaliP27835.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the WzyE family.UniRule annotationCurated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105DAD. Bacteria.
ENOG410XT3V. LUCA.
HOGENOMiHOG000219137.
KOiK02853.
OMAiWLWPDRP.

Family and domain databases

HAMAPiMF_01003. WzyE. 1 hit.
InterProiIPR010691. WzyE.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P27835-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLLQFSGLF VVWLLCTLFI ATLTWFEFRR VRFNFNVFFS LLFLLTFFFG
60 70 80 90 100
FPLTSVLVFR FDVGVAPPEI LLQALLSAGC FYAVYYVTYK TRLRKRVADV
110 120 130 140 150
PRRPLFTMNR VETNLTWVIL MGIALVSVGI FFMHNGFLLF RLNSYSQIFS
160 170 180 190 200
SEVSGVALKR FFYFFIPAML VVYFLRQDSK AWLFFLVSTV AFGLLTYMIV
210 220 230 240 250
GGTRANIIIA FAIFLFIGII RGWISLWMLA AAGVLGIVGM FWLALKRYGM
260 270 280 290 300
NVSGDEAFYT FLYLTRDTFS PWENLALLLQ NYDNIDFQGL APIVRDFYVF
310 320 330 340 350
IPSWLWPGRP SMVLNSANYF TWEVLNNHSG LAISPTLIGS LVVMGGALFI
360 370 380 390 400
PLGAIVVGLI IKWFDWLYEL GNREPNRYKA AILHSFCFGA IFNMIVLARE
410 420 430 440 450
GLDSFVSRVV FFIVVFGACL MIAKLLYWLF ESAGLIHKRT KSSLRTQVEG
Length:450
Mass (Da):51,517
Last modified:July 15, 1998 - v3
Checksum:i9B6B5D2CA8BED3DE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti283 – 2831D → H in AAK62973 (PubMed:11673418).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF375882 Genomic DNA. Translation: AAK62973.1.
M87049 Genomic DNA. Translation: AAA67593.1. Frameshift.
U00096 Genomic DNA. Translation: AAC76800.1.
AP009048 Genomic DNA. Translation: BAE77504.1.
PIRiF65183.
S30687.
RefSeqiNP_418241.1. NC_000913.3.
WP_000055129.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76800; AAC76800; b3793.
BAE77504; BAE77504; BAE77504.
GeneIDi948293.
KEGGiecj:JW3769.
eco:b3793.
PATRICi32123081. VBIEscCol129921_3910.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF375882 Genomic DNA. Translation: AAK62973.1.
M87049 Genomic DNA. Translation: AAA67593.1. Frameshift.
U00096 Genomic DNA. Translation: AAC76800.1.
AP009048 Genomic DNA. Translation: BAE77504.1.
PIRiF65183.
S30687.
RefSeqiNP_418241.1. NC_000913.3.
WP_000055129.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP27835.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263518. 285 interactions.
STRINGi511145.b3793.

Proteomic databases

PaxDbiP27835.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76800; AAC76800; b3793.
BAE77504; BAE77504; BAE77504.
GeneIDi948293.
KEGGiecj:JW3769.
eco:b3793.
PATRICi32123081. VBIEscCol129921_3910.

Organism-specific databases

EchoBASEiEB1426.
EcoGeneiEG11457. wzyE.

Phylogenomic databases

eggNOGiENOG4105DAD. Bacteria.
ENOG410XT3V. LUCA.
HOGENOMiHOG000219137.
KOiK02853.
OMAiWLWPDRP.

Enzyme and pathway databases

UniPathwayiUPA00566.
BioCyciEcoCyc:FUC4NACTRANS-MONOMER.
ECOL316407:JW3769-MONOMER.
MetaCyc:FUC4NACTRANS-MONOMER.

Miscellaneous databases

PROiP27835.

Family and domain databases

HAMAPiMF_01003. WzyE. 1 hit.
InterProiIPR010691. WzyE.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiWZYE_ECOLI
AccessioniPrimary (citable) accession number: P27835
Secondary accession number(s): Q2M8A2, Q93DX6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: July 15, 1998
Last modified: September 7, 2016
This is version 116 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to be WecF, the 4-alpha-L-fucosyltransferase.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.