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Protein

dTDP-4-amino-4,6-dideoxygalactose transaminase

Gene

wecE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the synthesis of dTDP-4-amino-4,6-dideoxy-D-galactose (dTDP-Fuc4N) from dTDP-4-keto-6-deoxy-D-glucose (dTDP-D-Glc4O) and L-glutamate.UniRule annotation1 Publication

Catalytic activityi

dTDP-4-amino-4,6-dideoxy-alpha-D-galactose + 2-oxoglutarate = dTDP-4-dehydro-6-deoxy-alpha-D-galactose + L-glutamate.UniRule annotation1 Publication

Cofactori

pyridoxal 5'-phosphateUniRule annotation1 Publication

Kineticsi

kcat is 0.38 sec(-1).1 Publication

Manual assertion based on experiment ini

  1. KM=0.11 mM for TDP-4-keto-6-deoxy-D-glucose1 Publication

    pH dependencei

    Optimum pH is 7.5.1 Publication

    Temperature dependencei

    Optimum temperature is 37 degrees Celsius.1 Publication

    Pathwayi: enterobacterial common antigen biosynthesis

    This protein is involved in the pathway enterobacterial common antigen biosynthesis, which is part of Bacterial outer membrane biogenesis.UniRule annotation1 Publication
    View all proteins of this organism that are known to be involved in the pathway enterobacterial common antigen biosynthesis and in Bacterial outer membrane biogenesis.

    GO - Molecular functioni

    GO - Biological processi

    • enterobacterial common antigen biosynthetic process Source: EcoCyc
    Complete GO annotation...

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    BioCyciEcoCyc:RFFTRANS-MONOMER.
    ECOL316407:JW3765-MONOMER.
    MetaCyc:RFFTRANS-MONOMER.
    UniPathwayiUPA00566.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    dTDP-4-amino-4,6-dideoxygalactose transaminaseUniRule annotationCurated (EC:2.6.1.59UniRule annotation1 Publication)
    Gene namesi
    Name:wecE1 PublicationUniRule annotation
    Synonyms:rffA1 Publication, yifI
    Ordered Locus Names:b3791, JW3765
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG11456. wecE.

    Pathology & Biotechi

    Disruption phenotypei

    Mutants do not synthesize TDP-Fuc4NAc and enterobacterial common antigen (ECA).1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001100161 – 376dTDP-4-amino-4,6-dideoxygalactose transaminaseAdd BLAST376

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei181N6-(pyridoxal phosphate)lysineUniRule annotationBy similarity1

    Proteomic databases

    EPDiP27833.
    PaxDbiP27833.
    PRIDEiP27833.

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    Protein-protein interaction databases

    BioGridi4263175. 298 interactors.
    IntActiP27833. 4 interactors.
    STRINGi511145.b3791.

    Structurei

    Secondary structure

    1376
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Helixi13 – 23Combined sources11
    Beta strandi27 – 29Combined sources3
    Helixi31 – 44Combined sources14
    Beta strandi49 – 53Combined sources5
    Helixi55 – 65Combined sources11
    Beta strandi73 – 80Combined sources8
    Helixi82 – 89Combined sources8
    Turni90 – 92Combined sources3
    Beta strandi94 – 98Combined sources5
    Turni102 – 104Combined sources3
    Helixi113 – 115Combined sources3
    Beta strandi120 – 123Combined sources4
    Helixi128 – 130Combined sources3
    Helixi135 – 144Combined sources10
    Beta strandi148 – 152Combined sources5
    Beta strandi168 – 176Combined sources9
    Beta strandi181 – 183Combined sources3
    Helixi185 – 187Combined sources3
    Beta strandi190 – 194Combined sources5
    Helixi197 – 199Combined sources3
    Helixi200 – 207Combined sources8
    Beta strandi208 – 210Combined sources3
    Helixi237 – 248Combined sources12
    Helixi250 – 273Combined sources24
    Beta strandi276 – 278Combined sources3
    Beta strandi294 – 296Combined sources3
    Helixi300 – 312Combined sources13
    Turni324 – 326Combined sources3
    Helixi328 – 333Combined sources6
    Beta strandi334 – 336Combined sources3
    Helixi343 – 349Combined sources7
    Beta strandi350 – 352Combined sources3
    Helixi361 – 374Combined sources14

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    4PIWX-ray2.70A/B/C/D/E/F/G/H1-376[»]
    4ZAHX-ray2.24A/B/C/D/E/F/G/H1-376[»]
    ProteinModelPortaliP27833.
    SMRiP27833.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the DegT/DnrJ/EryC1 family.UniRule annotationCurated

    Phylogenomic databases

    eggNOGiENOG4105CF4. Bacteria.
    COG0399. LUCA.
    HOGENOMiHOG000230162.
    InParanoidiP27833.
    KOiK02805.
    OMAiRQALINW.
    PhylomeDBiP27833.

    Family and domain databases

    CDDicd00616. AHBA_syn. 1 hit.
    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    HAMAPiMF_02026. WecE_RffA. 1 hit.
    InterProiIPR000653. DegT/StrS_aminotransferase.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    IPR032894. WecE.
    IPR012749. WecE-like.
    [Graphical view]
    PfamiPF01041. DegT_DnrJ_EryC1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000390. PLP_StrS. 1 hit.
    SUPFAMiSSF53383. SSF53383. 1 hit.
    TIGRFAMsiTIGR02379. ECA_wecE. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P27833-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MIPFNAPPVV GTELDYMQSA MGSGKLCGDG GFTRRCQQWL EQRFGSAKVL
    60 70 80 90 100
    LTPSCTASLE MAALLLDIQP GDEVIMPSYT FVSTANAFVL RGAKIVFVDV
    110 120 130 140 150
    RPDTMNIDET LIEAAITDKT RVIVPVHYAG VACEMDTIMA LAKKHNLFVV
    160 170 180 190 200
    EDAAQGVMST YKGRALGTIG HIGCFSFHET KNYTAGGEGG ATLINDKALI
    210 220 230 240 250
    ERAEIIREKG TNRSQFFRGQ VDKYTWRDIG SSYLMSDLQA AYLWAQLEAA
    260 270 280 290 300
    DRINQQRLAL WQNYYDALAP LAKAGRIELP SIPDGCVQNA HMFYIKLRDI
    310 320 330 340 350
    DDRSALINFL KEAEIMAVFH YIPLHGCPAG EHFGEFHGED RYTTKESERL
    360 370
    LRLPLFYNLS PVNQRTVIAT LLNYFS
    Length:376
    Mass (Da):41,901
    Last modified:July 15, 1998 - v2
    Checksum:i22936C67822D1844
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M87049 Genomic DNA. Translation: AAA67591.1. Frameshift.
    U00096 Genomic DNA. Translation: AAC76796.1.
    AP009048 Genomic DNA. Translation: BAE77507.1.
    PIRiB65183.
    RefSeqiNP_418238.1. NC_000913.3.
    WP_000612043.1. NZ_LN832404.1.

    Genome annotation databases

    EnsemblBacteriaiAAC76796; AAC76796; b3791.
    BAE77507; BAE77507; BAE77507.
    GeneIDi948296.
    KEGGiecj:JW3765.
    eco:b3791.
    PATRICi32123075. VBIEscCol129921_3907.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M87049 Genomic DNA. Translation: AAA67591.1. Frameshift.
    U00096 Genomic DNA. Translation: AAC76796.1.
    AP009048 Genomic DNA. Translation: BAE77507.1.
    PIRiB65183.
    RefSeqiNP_418238.1. NC_000913.3.
    WP_000612043.1. NZ_LN832404.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    4PIWX-ray2.70A/B/C/D/E/F/G/H1-376[»]
    4ZAHX-ray2.24A/B/C/D/E/F/G/H1-376[»]
    ProteinModelPortaliP27833.
    SMRiP27833.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4263175. 298 interactors.
    IntActiP27833. 4 interactors.
    STRINGi511145.b3791.

    Proteomic databases

    EPDiP27833.
    PaxDbiP27833.
    PRIDEiP27833.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC76796; AAC76796; b3791.
    BAE77507; BAE77507; BAE77507.
    GeneIDi948296.
    KEGGiecj:JW3765.
    eco:b3791.
    PATRICi32123075. VBIEscCol129921_3907.

    Organism-specific databases

    EchoBASEiEB1425.
    EcoGeneiEG11456. wecE.

    Phylogenomic databases

    eggNOGiENOG4105CF4. Bacteria.
    COG0399. LUCA.
    HOGENOMiHOG000230162.
    InParanoidiP27833.
    KOiK02805.
    OMAiRQALINW.
    PhylomeDBiP27833.

    Enzyme and pathway databases

    UniPathwayiUPA00566.
    BioCyciEcoCyc:RFFTRANS-MONOMER.
    ECOL316407:JW3765-MONOMER.
    MetaCyc:RFFTRANS-MONOMER.

    Miscellaneous databases

    PROiP27833.

    Family and domain databases

    CDDicd00616. AHBA_syn. 1 hit.
    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    HAMAPiMF_02026. WecE_RffA. 1 hit.
    InterProiIPR000653. DegT/StrS_aminotransferase.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    IPR032894. WecE.
    IPR012749. WecE-like.
    [Graphical view]
    PfamiPF01041. DegT_DnrJ_EryC1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000390. PLP_StrS. 1 hit.
    SUPFAMiSSF53383. SSF53383. 1 hit.
    TIGRFAMsiTIGR02379. ECA_wecE. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiWECE_ECOLI
    AccessioniPrimary (citable) accession number: P27833
    Secondary accession number(s): Q2M899
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: July 15, 1998
    Last modified: November 2, 2016
    This is version 124 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.