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Protein

dTDP-fucosamine acetyltransferase

Gene

wecD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the acetylation of dTDP-fucosamine (dTDP-4-amino-4,6-dideoxy-D-galactose) to dTDP-Fuc4NAc, which is utilized in the biosynthesis of the enterobacterial common antigen (ECA).UniRule annotation

Catalytic activityi

Acetyl-CoA + dTDP-4-amino-4,6-dideoxy-alpha-D-galactose = CoA + dTDP-4-acetamido-4,6-dideoxy-alpha-D-galactose.UniRule annotation

Pathwayi: enterobacterial common antigen biosynthesis

This protein is involved in the pathway enterobacterial common antigen biosynthesis, which is part of Bacterial outer membrane biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway enterobacterial common antigen biosynthesis and in Bacterial outer membrane biogenesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei201 – 2011Acetyl-CoAUniRule annotation
Binding sitei207 – 2071Acetyl-CoAUniRule annotation
Active sitei208 – 2081Proton donorUniRule annotation

GO - Molecular functioni

  • N-acetyltransferase activity Source: EcoCyc

GO - Biological processi

  • enterobacterial common antigen biosynthetic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

BioCyciEcoCyc:TDPFUCACTRANS-MONOMER.
ECOL316407:JW5597-MONOMER.
MetaCyc:TDPFUCACTRANS-MONOMER.
UniPathwayiUPA00566.

Names & Taxonomyi

Protein namesi
Recommended name:
dTDP-fucosamine acetyltransferaseUniRule annotation (EC:2.3.1.210UniRule annotation)
Alternative name(s):
TDP-fucosamine acetyltransferaseUniRule annotation
dTDP-4-amino-4,6-dideoxy-D-galactose acyltransferaseUniRule annotation
Gene namesi
Name:wecDUniRule annotation
Synonyms:rffC1 Publication, yifH
Ordered Locus Names:b3790, JW5597
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11455. wecD.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 224224dTDP-fucosamine acetyltransferasePRO_0000067230Add
BLAST

Proteomic databases

PaxDbiP27832.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

BioGridi4261646. 249 interactions.
STRINGi511145.b3790.

Structurei

3D structure databases

ProteinModelPortaliP27832.
SMRiP27832. Positions 3-224.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini94 – 224131N-acetyltransferaseUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni168 – 1747Acetyl-CoA bindingUniRule annotation

Sequence similaritiesi

Belongs to the WecD family.UniRule annotation
Contains 1 N-acetyltransferase domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108WJ1. Bacteria.
COG0454. LUCA.
HOGENOMiHOG000126448.
KOiK16704.
OMAiTAYWFYR.
OrthoDBiEOG693GN2.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
HAMAPiMF_02027. WecD_RffC.
InterProiIPR012752. AcTrfase_WecD.
IPR016181. Acyl_CoA_acyltransferase.
IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR000182. GNAT_dom.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 1 hit.
TIGRFAMsiTIGR02382. wecD_rffC. 1 hit.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P27832-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPVRASIEPL TWENAFFGVN SAIVRITSEA PLLTPDALAP WSRVQAKIAA
60 70 80 90 100
SNTGELDALQ QLGFSLVEGE VDLALPVNNA SDSGAVVAQE TDIPALRQLA
110 120 130 140 150
SAAFAQSRFR APWYAPDASS RFYAQWIENA VRGTFDHQCL ILRAASGDIR
160 170 180 190 200
GYVSLRELNA TDARIGLLAG RGAGAELMQT ALNWAYARGK TTLRVATQMG
210 220
NTAALKRYIQ SGANVESTAY WLYR
Length:224
Mass (Da):24,220
Last modified:July 10, 2007 - v3
Checksum:iA1B196D4DA00321B
GO

Sequence cautioni

The sequence AAA67590.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAE77508.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti187 – 1871A → R in AAA67590 (PubMed:1379743).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M87049 Genomic DNA. Translation: AAA67590.1. Different initiation.
U00096 Genomic DNA. Translation: AAT48214.2.
AP009048 Genomic DNA. Translation: BAE77508.1. Different initiation.
PIRiA65183.
RefSeqiWP_001145183.1. NZ_LN832404.1.
YP_026256.2. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAT48214; AAT48214; b3790.
BAE77508; BAE77508; BAE77508.
GeneIDi948298.
KEGGiecj:JW5597.
eco:b3790.
PATRICi32123073. VBIEscCol129921_3906.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M87049 Genomic DNA. Translation: AAA67590.1. Different initiation.
U00096 Genomic DNA. Translation: AAT48214.2.
AP009048 Genomic DNA. Translation: BAE77508.1. Different initiation.
PIRiA65183.
RefSeqiWP_001145183.1. NZ_LN832404.1.
YP_026256.2. NC_000913.3.

3D structure databases

ProteinModelPortaliP27832.
SMRiP27832. Positions 3-224.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261646. 249 interactions.
STRINGi511145.b3790.

Proteomic databases

PaxDbiP27832.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT48214; AAT48214; b3790.
BAE77508; BAE77508; BAE77508.
GeneIDi948298.
KEGGiecj:JW5597.
eco:b3790.
PATRICi32123073. VBIEscCol129921_3906.

Organism-specific databases

EchoBASEiEB1424.
EcoGeneiEG11455. wecD.

Phylogenomic databases

eggNOGiENOG4108WJ1. Bacteria.
COG0454. LUCA.
HOGENOMiHOG000126448.
KOiK16704.
OMAiTAYWFYR.
OrthoDBiEOG693GN2.

Enzyme and pathway databases

UniPathwayiUPA00566.
BioCyciEcoCyc:TDPFUCACTRANS-MONOMER.
ECOL316407:JW5597-MONOMER.
MetaCyc:TDPFUCACTRANS-MONOMER.

Miscellaneous databases

PROiP27832.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
HAMAPiMF_02027. WecD_RffC.
InterProiIPR012752. AcTrfase_WecD.
IPR016181. Acyl_CoA_acyltransferase.
IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR000182. GNAT_dom.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 1 hit.
TIGRFAMsiTIGR02382. wecD_rffC. 1 hit.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes."
    Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.
    Science 257:771-778(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: SEQUENCE REVISION TO 187.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Physical map location of the rffC and rffA genes of Escherichia coli."
    Barr K., Rick P.D.
    J. Bacteriol. 175:5738-5739(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.

Entry informationi

Entry nameiWECD_ECOLI
AccessioniPrimary (citable) accession number: P27832
Secondary accession number(s): Q2M898, Q6BF00
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: July 10, 2007
Last modified: January 20, 2016
This is version 121 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.