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Protein

UDP-N-acetyl-D-mannosamine dehydrogenase

Gene

wecC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the four-electron oxidation of UDP-N-acetyl-D-mannosamine (UDP-ManNAc), reducing NAD+ and releasing UDP-N-acetylmannosaminuronic acid (UDP-ManNAcA).UniRule annotation1 Publication

Catalytic activityi

UDP-N-acetyl-alpha-D-mannosamine + 2 NAD+ + H2O = UDP-N-acetyl-alpha-D-mannosaminuronate + 2 NADH.UniRule annotation1 Publication

Enzyme regulationi

Activated by N-acetylglucosamine-1-P or K+ at low UDP-ManNAc concentrations.1 Publication

Kineticsi

  1. KM=0.22 mM for UDP-ManNAc (in the presence of 1 mM N-acetylglucosamine-1-P)1 Publication
  2. KM=0.11 mM for UDP-ManNAc (in the presence of 150 mM KCl)1 Publication
  3. KM=0.38 mM for UDP-ManNAc (in the absence of activators)1 Publication
  4. KM=0.21 mM for NAD+1 Publication

    pH dependencei

    Optimum pH is 10.0.1 Publication

    Pathwayi: enterobacterial common antigen biosynthesis

    This protein is involved in the pathway enterobacterial common antigen biosynthesis, which is part of Bacterial outer membrane biogenesis.UniRule annotation1 Publication
    View all proteins of this organism that are known to be involved in the pathway enterobacterial common antigen biosynthesis and in Bacterial outer membrane biogenesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei212Proton donor/acceptorUniRule annotationBy similarity1
    Binding sitei212SubstrateUniRule annotationBy similarity1
    Binding sitei263Substrate; via amide nitrogenUniRule annotationBy similarity1
    Active sitei266NucleophileUniRule annotationBy similarity1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi10 – 15NADUniRule annotationBy similarity6

    GO - Molecular functioni

    GO - Biological processi

    • enterobacterial common antigen biosynthetic process Source: EcoCyc

    Keywordsi

    Molecular functionOxidoreductase
    LigandNAD

    Enzyme and pathway databases

    BioCyciEcoCyc:UDPMANNACADEHYDROG-MONOMER
    MetaCyc:UDPMANNACADEHYDROG-MONOMER
    UniPathwayiUPA00566

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UDP-N-acetyl-D-mannosamine dehydrogenase1 PublicationUniRule annotation (EC:1.1.1.336UniRule annotation1 Publication)
    Alternative name(s):
    UDP-ManNAc 6-dehydrogenaseUniRule annotationCurated
    Gene namesi
    Name:wecCUniRule annotationImported
    Synonyms:rffD1 Publication
    Ordered Locus Names:b3787, JW5599
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG11452 wecC

    Subcellular locationi

    GO - Cellular componenti

    • cytosol Source: EcoCyc

    Pathology & Biotechi

    Disruption phenotypei

    Mutants do not synthesize Und-PP-GlcNAc-ManNAcA (lipid II) and enterobacterial common antigen (ECA).1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000740771 – 420UDP-N-acetyl-D-mannosamine dehydrogenaseAdd BLAST420

    Proteomic databases

    EPDiP27829
    PaxDbiP27829
    PRIDEiP27829

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    BioGridi4262604, 229 interactors
    STRINGi316385.ECDH10B_3976

    Structurei

    3D structure databases

    ProteinModelPortaliP27829
    SMRiP27829
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni160 – 161SubstrateUniRule annotationBy similarity2
    Regioni216 – 219SubstrateUniRule annotationBy similarity4
    Regioni250 – 252Substrate; shared with dimeric partnerUniRule annotationBy similarity3
    Regioni330 – 331SubstrateUniRule annotationBy similarity2

    Sequence similaritiesi

    Belongs to the UDP-glucose/GDP-mannose dehydrogenase family. WecC subfamily.UniRule annotationCurated

    Phylogenomic databases

    eggNOGiENOG4107QQG Bacteria
    COG0677 LUCA
    HOGENOMiHOG000153774
    InParanoidiP27829
    KOiK02472
    OMAiVNQHAVD
    PhylomeDBiP27829

    Family and domain databases

    HAMAPiMF_02029 WecC_RffD, 1 hit
    InterProiView protein in InterPro
    IPR008927 6-PGluconate_DH-like_C_sf
    IPR036291 NAD(P)-bd_dom_sf
    IPR017476 UDP-Glc/GDP-Man
    IPR014027 UDP-Glc/GDP-Man_DH_C
    IPR036220 UDP-Glc/GDP-Man_DH_C_sf
    IPR014026 UDP-Glc/GDP-Man_DH_dimer
    IPR001732 UDP-Glc/GDP-Man_DH_N
    IPR028359 UDP_ManNAc/GlcNAc_DH
    IPR032891 WecC
    PfamiView protein in Pfam
    PF00984 UDPG_MGDP_dh, 1 hit
    PF03720 UDPG_MGDP_dh_C, 1 hit
    PF03721 UDPG_MGDP_dh_N, 1 hit
    PIRSFiPIRSF500136 UDP_ManNAc_DH, 1 hit
    PIRSF000124 UDPglc_GDPman_dh, 1 hit
    SMARTiView protein in SMART
    SM00984 UDPG_MGDP_dh_C, 1 hit
    SUPFAMiSSF48179 SSF48179, 1 hit
    SSF51735 SSF51735, 1 hit
    SSF52413 SSF52413, 1 hit
    TIGRFAMsiTIGR03026 NDP-sugDHase, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P27829-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSFATISVIG LGYIGLPTAA AFASRQKQVI GVDINQHAVD TINRGEIHIV
    60 70 80 90 100
    EPDLASVVKT AVEGGFLRAS TTPVEADAWL IAVPTPFKGD HEPDMTYVES
    110 120 130 140 150
    AARSIAPVLK KGALVILEST SPVGSTEKMA EWLAEMRPDL TFPQQVGEQA
    160 170 180 190 200
    DVNIAYCPER VLPGQVMVEL IKNDRVIGGM TPVCSARASE LYKIFLEGEC
    210 220 230 240 250
    VVTNSRTAEM CKLTENSFRD VNIAFANELS LICADQGINV WELIRLANRH
    260 270 280 290 300
    PRVNILQPGP GVGGHCIAVD PWFIVAQNPQ QARLIRTARE VNDHKPFWVI
    310 320 330 340 350
    DQVKAAVADC LAATDKRASE LKIACFGLAF KPNIDDLRES PAMEIAELIA
    360 370 380 390 400
    QWHSGETLVV EPNIHQLPKK LTGLCTLAQL DEALATADVL VMLVDHSQFK
    410 420
    VINGDNVHQQ YVVDAKGVWR
    Length:420
    Mass (Da):45,839
    Last modified:October 11, 2004 - v4
    Checksum:iDDD63F572FDEA463
    GO

    Sequence cautioni

    The sequence AAA67587 differs from that shown. Reason: Frameshift at position 375.Curated

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti78A → R in AAA67587 (PubMed:1379743).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    L18799 Unassigned DNA No translation available.
    M87049 Genomic DNA Translation: AAA67587.1 Frameshift.
    U00096 Genomic DNA Translation: AAT48212.1
    AP009048 Genomic DNA Translation: BAE77511.1
    PIRiF65182
    RefSeqiWP_000006621.1, NZ_LN832404.1
    YP_026254.1, NC_000913.3

    Genome annotation databases

    EnsemblBacteriaiAAT48212; AAT48212; b3787
    BAE77511; BAE77511; BAE77511
    GeneIDi948977
    KEGGiecj:JW5599
    eco:b3787
    PATRICifig|1411691.4.peg.2919

    Similar proteinsi

    Entry informationi

    Entry nameiWECC_ECOLI
    AccessioniPrimary (citable) accession number: P27829
    Secondary accession number(s): Q2M895
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: October 11, 2004
    Last modified: March 28, 2018
    This is version 150 of the entry and version 4 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health