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Protein

UDP-N-acetyl-D-mannosamine dehydrogenase

Gene

wecC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the four-electron oxidation of UDP-N-acetyl-D-mannosamine (UDP-ManNAc), reducing NAD+ and releasing UDP-N-acetylmannosaminuronic acid (UDP-ManNAcA).UniRule annotation1 Publication

Catalytic activityi

UDP-N-acetyl-alpha-D-mannosamine + 2 NAD+ + H2O = UDP-N-acetyl-alpha-D-mannosaminuronate + 2 NADH.UniRule annotation1 Publication

Enzyme regulationi

Activated by N-acetylglucosamine-1-P or K+ at low UDP-ManNAc concentrations.1 Publication

Kineticsi

  1. KM=0.22 mM for UDP-ManNAc (in the presence of 1 mM N-acetylglucosamine-1-P)1 Publication
  2. KM=0.11 mM for UDP-ManNAc (in the presence of 150 mM KCl)1 Publication
  3. KM=0.38 mM for UDP-ManNAc (in the absence of activators)1 Publication
  4. KM=0.21 mM for NAD+1 Publication

    pH dependencei

    Optimum pH is 10.0.1 Publication

    Pathwayi: enterobacterial common antigen biosynthesis

    This protein is involved in the pathway enterobacterial common antigen biosynthesis, which is part of Bacterial outer membrane biogenesis.UniRule annotation1 Publication
    View all proteins of this organism that are known to be involved in the pathway enterobacterial common antigen biosynthesis and in Bacterial outer membrane biogenesis.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei266 – 2661NucleophileUniRule annotationBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi10 – 156NADUniRule annotationBy similarity

    GO - Molecular functioni

    GO - Biological processi

    • enterobacterial common antigen biosynthetic process Source: EcoCyc
    • UDP-glucuronate biosynthetic process Source: GO_Central
    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciEcoCyc:UDPMANNACADEHYDROG-MONOMER.
    ECOL316407:JW5599-MONOMER.
    MetaCyc:UDPMANNACADEHYDROG-MONOMER.
    UniPathwayiUPA00566.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UDP-N-acetyl-D-mannosamine dehydrogenase1 PublicationUniRule annotation (EC:1.1.1.336UniRule annotation1 Publication)
    Alternative name(s):
    UDP-ManNAc 6-dehydrogenaseUniRule annotationCurated
    Gene namesi
    Name:wecCUniRule annotationImported
    Synonyms:rffD1 Publication
    Ordered Locus Names:b3787, JW5599
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG11452. wecC.

    Subcellular locationi

    GO - Cellular componenti

    • cytosol Source: EcoCyc
    Complete GO annotation...

    Pathology & Biotechi

    Disruption phenotypei

    Mutants do not synthesize Und-PP-GlcNAc-ManNAcA (lipid II) and enterobacterial common antigen (ECA).1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 420420UDP-N-acetyl-D-mannosamine dehydrogenasePRO_0000074077Add
    BLAST

    Proteomic databases

    EPDiP27829.
    PaxDbiP27829.
    PRIDEiP27829.

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    BioGridi4262604. 220 interactions.
    STRINGi511145.b3787.

    Structurei

    3D structure databases

    ProteinModelPortaliP27829.
    SMRiP27829. Positions 6-399.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the UDP-glucose/GDP-mannose dehydrogenase family. WecC subfamily.UniRule annotationCurated

    Phylogenomic databases

    eggNOGiENOG4107QQG. Bacteria.
    COG0677. LUCA.
    HOGENOMiHOG000153774.
    InParanoidiP27829.
    KOiK02472.
    OMAiIQQKRVD.
    PhylomeDBiP27829.

    Family and domain databases

    Gene3Di3.40.50.720. 2 hits.
    HAMAPiMF_02029. WecC_RffD. 1 hit.
    InterProiIPR008927. 6-PGluconate_DH_C-like.
    IPR016040. NAD(P)-bd_dom.
    IPR017476. UDP-Glc/GDP-Man.
    IPR014027. UDP-Glc/GDP-Man_DH_C.
    IPR014026. UDP-Glc/GDP-Man_DH_dimer.
    IPR001732. UDP-Glc/GDP-Man_DH_N.
    IPR028359. UDP_ManNAc/GlcNAc_DH.
    IPR032891. WecC.
    [Graphical view]
    PANTHERiPTHR11374. PTHR11374. 1 hit.
    PfamiPF00984. UDPG_MGDP_dh. 1 hit.
    PF03720. UDPG_MGDP_dh_C. 1 hit.
    PF03721. UDPG_MGDP_dh_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF500136. UDP_ManNAc_DH. 1 hit.
    PIRSF000124. UDPglc_GDPman_dh. 1 hit.
    SMARTiSM00984. UDPG_MGDP_dh_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF48179. SSF48179. 1 hit.
    SSF51735. SSF51735. 1 hit.
    SSF52413. SSF52413. 1 hit.
    TIGRFAMsiTIGR03026. NDP-sugDHase. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P27829-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSFATISVIG LGYIGLPTAA AFASRQKQVI GVDINQHAVD TINRGEIHIV
    60 70 80 90 100
    EPDLASVVKT AVEGGFLRAS TTPVEADAWL IAVPTPFKGD HEPDMTYVES
    110 120 130 140 150
    AARSIAPVLK KGALVILEST SPVGSTEKMA EWLAEMRPDL TFPQQVGEQA
    160 170 180 190 200
    DVNIAYCPER VLPGQVMVEL IKNDRVIGGM TPVCSARASE LYKIFLEGEC
    210 220 230 240 250
    VVTNSRTAEM CKLTENSFRD VNIAFANELS LICADQGINV WELIRLANRH
    260 270 280 290 300
    PRVNILQPGP GVGGHCIAVD PWFIVAQNPQ QARLIRTARE VNDHKPFWVI
    310 320 330 340 350
    DQVKAAVADC LAATDKRASE LKIACFGLAF KPNIDDLRES PAMEIAELIA
    360 370 380 390 400
    QWHSGETLVV EPNIHQLPKK LTGLCTLAQL DEALATADVL VMLVDHSQFK
    410 420
    VINGDNVHQQ YVVDAKGVWR
    Length:420
    Mass (Da):45,839
    Last modified:October 11, 2004 - v4
    Checksum:iDDD63F572FDEA463
    GO

    Sequence cautioni

    The sequence AAA67587 differs from that shown. Reason: Frameshift at position 375. Curated

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti78 – 781A → R in AAA67587 (PubMed:1379743).Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    L18799 Unassigned DNA. No translation available.
    M87049 Genomic DNA. Translation: AAA67587.1. Frameshift.
    U00096 Genomic DNA. Translation: AAT48212.1.
    AP009048 Genomic DNA. Translation: BAE77511.1.
    PIRiF65182.
    RefSeqiWP_000006621.1. NZ_LN832404.1.
    YP_026254.1. NC_000913.3.

    Genome annotation databases

    EnsemblBacteriaiAAT48212; AAT48212; b3787.
    BAE77511; BAE77511; BAE77511.
    GeneIDi948977.
    KEGGiecj:JW5599.
    eco:b3787.
    PATRICi32123067. VBIEscCol129921_3903.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    L18799 Unassigned DNA. No translation available.
    M87049 Genomic DNA. Translation: AAA67587.1. Frameshift.
    U00096 Genomic DNA. Translation: AAT48212.1.
    AP009048 Genomic DNA. Translation: BAE77511.1.
    PIRiF65182.
    RefSeqiWP_000006621.1. NZ_LN832404.1.
    YP_026254.1. NC_000913.3.

    3D structure databases

    ProteinModelPortaliP27829.
    SMRiP27829. Positions 6-399.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4262604. 220 interactions.
    STRINGi511145.b3787.

    Proteomic databases

    EPDiP27829.
    PaxDbiP27829.
    PRIDEiP27829.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAT48212; AAT48212; b3787.
    BAE77511; BAE77511; BAE77511.
    GeneIDi948977.
    KEGGiecj:JW5599.
    eco:b3787.
    PATRICi32123067. VBIEscCol129921_3903.

    Organism-specific databases

    EchoBASEiEB1421.
    EcoGeneiEG11452. wecC.

    Phylogenomic databases

    eggNOGiENOG4107QQG. Bacteria.
    COG0677. LUCA.
    HOGENOMiHOG000153774.
    InParanoidiP27829.
    KOiK02472.
    OMAiIQQKRVD.
    PhylomeDBiP27829.

    Enzyme and pathway databases

    UniPathwayiUPA00566.
    BioCyciEcoCyc:UDPMANNACADEHYDROG-MONOMER.
    ECOL316407:JW5599-MONOMER.
    MetaCyc:UDPMANNACADEHYDROG-MONOMER.

    Miscellaneous databases

    PROiP27829.

    Family and domain databases

    Gene3Di3.40.50.720. 2 hits.
    HAMAPiMF_02029. WecC_RffD. 1 hit.
    InterProiIPR008927. 6-PGluconate_DH_C-like.
    IPR016040. NAD(P)-bd_dom.
    IPR017476. UDP-Glc/GDP-Man.
    IPR014027. UDP-Glc/GDP-Man_DH_C.
    IPR014026. UDP-Glc/GDP-Man_DH_dimer.
    IPR001732. UDP-Glc/GDP-Man_DH_N.
    IPR028359. UDP_ManNAc/GlcNAc_DH.
    IPR032891. WecC.
    [Graphical view]
    PANTHERiPTHR11374. PTHR11374. 1 hit.
    PfamiPF00984. UDPG_MGDP_dh. 1 hit.
    PF03720. UDPG_MGDP_dh_C. 1 hit.
    PF03721. UDPG_MGDP_dh_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF500136. UDP_ManNAc_DH. 1 hit.
    PIRSF000124. UDPglc_GDPman_dh. 1 hit.
    SMARTiSM00984. UDPG_MGDP_dh_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF48179. SSF48179. 1 hit.
    SSF51735. SSF51735. 1 hit.
    SSF52413. SSF52413. 1 hit.
    TIGRFAMsiTIGR03026. NDP-sugDHase. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiWECC_ECOLI
    AccessioniPrimary (citable) accession number: P27829
    Secondary accession number(s): Q2M895
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: October 11, 2004
    Last modified: September 7, 2016
    This is version 137 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.