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Protein

Calnexin homolog

Gene

CNE1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei131 – 1311CarbohydrateBy similarity

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • carbohydrate binding Source: UniProtKB-KW
  • unfolded protein binding Source: SGD

GO - Biological processi

  • ER-associated ubiquitin-dependent protein catabolic process Source: SGD
  • protein folding Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Ligandi

Lectin

Enzyme and pathway databases

BioCyciYEAST:G3O-28861-MONOMER.
ReactomeiR-SCE-901042. Calnexin/calreticulin cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Calnexin homolog
Gene namesi
Name:CNE1
Ordered Locus Names:YAL058W
ORF Names:FUN48
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:YAL058W.
SGDiS000000054. CNE1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini20 – 481462LumenalSequence analysisAdd
BLAST
Transmembranei482 – 50221HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of endoplasmic reticulum membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence analysisAdd
BLAST
Chaini20 – 502483Calnexin homologPRO_0000004208Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi25 – 251N-linked (GlcNAc...)Sequence analysis
Glycosylationi104 – 1041N-linked (GlcNAc...)Sequence analysis
Disulfide bondi125 ↔ 161By similarity
Glycosylationi296 – 2961N-linked (GlcNAc...)Sequence analysis
Disulfide bondi332 ↔ 338By similarity
Glycosylationi416 – 4161N-linked (GlcNAc...)Sequence analysis
Glycosylationi425 – 4251N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP27825.
TopDownProteomicsiP27825.

Interactioni

Subunit structurei

Interacts with MPD1.1 Publication

GO - Molecular functioni

  • unfolded protein binding Source: SGD

Protein-protein interaction databases

BioGridi31772. 139 interactions.
DIPiDIP-4121N.
MINTiMINT-532274.

Structurei

3D structure databases

ProteinModelPortaliP27825.
SMRiP27825. Positions 33-379.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati250 – 261121-1Add
BLAST
Repeati267 – 278121-2Add
BLAST
Repeati286 – 297121-3Add
BLAST
Repeati305 – 316121-4Add
BLAST
Repeati320 – 330112-1Add
BLAST
Repeati339 – 349112-2Add
BLAST
Repeati353 – 363112-3Add
BLAST
Repeati367 – 377112-4Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni248 – 381134P domain (Extended arm)By similarityAdd
BLAST
Regioni250 – 316674 X approximate repeatsAdd
BLAST
Regioni320 – 377584 X approximate repeatsAdd
BLAST

Sequence similaritiesi

Belongs to the calreticulin family.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00430000030841.
InParanoidiP27825.
KOiK08054.
OMAiEWITSEA.
OrthoDBiEOG092C3PLH.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR001580. Calret/calnex.
IPR018124. Calret/calnex_CS.
IPR009033. Calreticulin/calnexin_P_dom.
IPR013320. ConA-like_dom.
[Graphical view]
PANTHERiPTHR11073. PTHR11073. 1 hit.
PfamiPF00262. Calreticulin. 1 hit.
[Graphical view]
PRINTSiPR00626. CALRETICULIN.
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF63887. SSF63887. 1 hit.
PROSITEiPS00803. CALRETICULIN_1. 1 hit.
PS00804. CALRETICULIN_2. 1 hit.
PS00805. CALRETICULIN_REPEAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27825-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFSAYLWWL FLNLALVKGT SLLSNVTLAE DSFWEHFQAY TNTKHLNQEW
60 70 80 90 100
ITSEAVNNEG SKIYGAQWRL SQGRLQGSAW DKGIAVRTGN AAAMIGHLLE
110 120 130 140 150
TPINVSETDT LVVQYEIKLD NSLTCGGAFI KLMSGFMNVE ALKHYAPDTE
160 170 180 190 200
GVELVFGPDY CAPEINGVQF AINKVDKITH ESKLRYLQEM PLSKLTDTSQ
210 220 230 240 250
SHLYTLIIDE SAQSFQILID GKTVMVREHI EDKKKVNFEP PITPPLMIPD
260 270 280 290 300
VSVAKPHDWD DRIRIPDPEA VKLSDRDERD PLMIPHPDGT EPPEWNSSIP
310 320 330 340 350
EYILDPNAQK PSWWKELEHG EWIPPMIKNP LCTAERGCGQ QIPGLINNAK
360 370 380 390 400
YKGPGELNEI INPNYMGEWH PPEIENPLYY EEQHPLRIEN VISGVILEFW
410 420 430 440 450
SGSPNMLISN IYVGKNVTEA QIIGNKTWLM RDRAFRGSDG PTERKFMNSR
460 470 480 490 500
LGNLQTTFHN ERESPNPFDR IIDRILEQPL KFVLTAAVVL LTTSVLCCVV

FT
Length:502
Mass (Da):56,968
Last modified:August 1, 1992 - v1
Checksum:i7A99030C147AB2F8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66470 Genomic DNA. Translation: CAA47100.1.
L11012 Genomic DNA. Translation: AAA65967.1.
U12980 Genomic DNA. Translation: AAC04976.1.
AY692920 Genomic DNA. Translation: AAT92939.1.
BK006935 Genomic DNA. Translation: DAA06931.1.
PIRiS29347.
RefSeqiNP_009343.1. NM_001178200.1.

Genome annotation databases

EnsemblFungiiYAL058W; YAL058W; YAL058W.
GeneIDi851241.
KEGGisce:YAL058W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66470 Genomic DNA. Translation: CAA47100.1.
L11012 Genomic DNA. Translation: AAA65967.1.
U12980 Genomic DNA. Translation: AAC04976.1.
AY692920 Genomic DNA. Translation: AAT92939.1.
BK006935 Genomic DNA. Translation: DAA06931.1.
PIRiS29347.
RefSeqiNP_009343.1. NM_001178200.1.

3D structure databases

ProteinModelPortaliP27825.
SMRiP27825. Positions 33-379.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31772. 139 interactions.
DIPiDIP-4121N.
MINTiMINT-532274.

Proteomic databases

MaxQBiP27825.
TopDownProteomicsiP27825.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYAL058W; YAL058W; YAL058W.
GeneIDi851241.
KEGGisce:YAL058W.

Organism-specific databases

EuPathDBiFungiDB:YAL058W.
SGDiS000000054. CNE1.

Phylogenomic databases

GeneTreeiENSGT00430000030841.
InParanoidiP27825.
KOiK08054.
OMAiEWITSEA.
OrthoDBiEOG092C3PLH.

Enzyme and pathway databases

BioCyciYEAST:G3O-28861-MONOMER.
ReactomeiR-SCE-901042. Calnexin/calreticulin cycle.

Miscellaneous databases

PROiP27825.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR001580. Calret/calnex.
IPR018124. Calret/calnex_CS.
IPR009033. Calreticulin/calnexin_P_dom.
IPR013320. ConA-like_dom.
[Graphical view]
PANTHERiPTHR11073. PTHR11073. 1 hit.
PfamiPF00262. Calreticulin. 1 hit.
[Graphical view]
PRINTSiPR00626. CALRETICULIN.
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF63887. SSF63887. 1 hit.
PROSITEiPS00803. CALRETICULIN_1. 1 hit.
PS00804. CALRETICULIN_2. 1 hit.
PS00805. CALRETICULIN_REPEAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCALX_YEAST
AccessioniPrimary (citable) accession number: P27825
Secondary accession number(s): D6VPG1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: September 7, 2016
This is version 146 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

In contrast with other calnexin, yeast CNE1 does not seem to bind calcium.
Present with 4760 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome I
    Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.