Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Calnexin homolog

Gene

CNE1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins.1 Publication

Miscellaneous

In contrast with other calnexin, yeast CNE1 does not seem to bind calcium.
Present with 4760 molecules/cell in log phase SD medium.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei131CarbohydrateBy similarity1

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • carbohydrate binding Source: UniProtKB-KW
  • unfolded protein binding Source: SGD

GO - Biological processi

  • protein folding Source: SGD
  • ubiquitin-dependent ERAD pathway Source: SGD

Keywordsi

Molecular functionChaperone
LigandLectin

Enzyme and pathway databases

BioCyciYEAST:G3O-28861-MONOMER
ReactomeiR-SCE-901042 Calnexin/calreticulin cycle

Names & Taxonomyi

Protein namesi
Recommended name:
Calnexin homolog
Gene namesi
Name:CNE1
Ordered Locus Names:YAL058W
ORF Names:FUN48
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:YAL058W
SGDiS000000054 CNE1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini20 – 481LumenalSequence analysisAdd BLAST462
Transmembranei482 – 502HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000000420820 – 502Calnexin homologAdd BLAST483

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi25N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi104N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi125 ↔ 161By similarity
Glycosylationi296N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi332 ↔ 338By similarity
Glycosylationi416N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi425N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP27825
PaxDbiP27825
PRIDEiP27825
TopDownProteomicsiP27825

Interactioni

Subunit structurei

Interacts with MPD1.1 Publication

GO - Molecular functioni

  • unfolded protein binding Source: SGD

Protein-protein interaction databases

BioGridi31772, 196 interactors
DIPiDIP-4121N
IntActiP27825, 1 interactor
STRINGi4932.YAL058W

Structurei

3D structure databases

ProteinModelPortaliP27825
SMRiP27825
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati250 – 2611-1Add BLAST12
Repeati267 – 2781-2Add BLAST12
Repeati286 – 2971-3Add BLAST12
Repeati305 – 3161-4Add BLAST12
Repeati320 – 3302-1Add BLAST11
Repeati339 – 3492-2Add BLAST11
Repeati353 – 3632-3Add BLAST11
Repeati367 – 3772-4Add BLAST11

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni248 – 381P domain (Extended arm)By similarityAdd BLAST134
Regioni250 – 3164 X approximate repeatsAdd BLAST67
Regioni320 – 3774 X approximate repeatsAdd BLAST58

Sequence similaritiesi

Belongs to the calreticulin family.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00430000030841
InParanoidiP27825
KOiK08054
OMAiIICADRR
OrthoDBiEOG092C3PLH

Family and domain databases

Gene3Di2.10.250.10, 1 hit
InterProiView protein in InterPro
IPR001580 Calret/calnex
IPR018124 Calret/calnex_CS
IPR009033 Calreticulin/calnexin_P_dom_sf
IPR013320 ConA-like_dom_sf
PANTHERiPTHR11073 PTHR11073, 1 hit
PfamiView protein in Pfam
PF00262 Calreticulin, 1 hit
PRINTSiPR00626 CALRETICULIN
SUPFAMiSSF49899 SSF49899, 1 hit
SSF63887 SSF63887, 1 hit
PROSITEiView protein in PROSITE
PS00803 CALRETICULIN_1, 1 hit
PS00804 CALRETICULIN_2, 1 hit
PS00805 CALRETICULIN_REPEAT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27825-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFSAYLWWL FLNLALVKGT SLLSNVTLAE DSFWEHFQAY TNTKHLNQEW
60 70 80 90 100
ITSEAVNNEG SKIYGAQWRL SQGRLQGSAW DKGIAVRTGN AAAMIGHLLE
110 120 130 140 150
TPINVSETDT LVVQYEIKLD NSLTCGGAFI KLMSGFMNVE ALKHYAPDTE
160 170 180 190 200
GVELVFGPDY CAPEINGVQF AINKVDKITH ESKLRYLQEM PLSKLTDTSQ
210 220 230 240 250
SHLYTLIIDE SAQSFQILID GKTVMVREHI EDKKKVNFEP PITPPLMIPD
260 270 280 290 300
VSVAKPHDWD DRIRIPDPEA VKLSDRDERD PLMIPHPDGT EPPEWNSSIP
310 320 330 340 350
EYILDPNAQK PSWWKELEHG EWIPPMIKNP LCTAERGCGQ QIPGLINNAK
360 370 380 390 400
YKGPGELNEI INPNYMGEWH PPEIENPLYY EEQHPLRIEN VISGVILEFW
410 420 430 440 450
SGSPNMLISN IYVGKNVTEA QIIGNKTWLM RDRAFRGSDG PTERKFMNSR
460 470 480 490 500
LGNLQTTFHN ERESPNPFDR IIDRILEQPL KFVLTAAVVL LTTSVLCCVV

FT
Length:502
Mass (Da):56,968
Last modified:August 1, 1992 - v1
Checksum:i7A99030C147AB2F8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66470 Genomic DNA Translation: CAA47100.1
L11012 Genomic DNA Translation: AAA65967.1
U12980 Genomic DNA Translation: AAC04976.1
AY692920 Genomic DNA Translation: AAT92939.1
BK006935 Genomic DNA Translation: DAA06931.1
PIRiS29347
RefSeqiNP_009343.1, NM_001178200.1

Genome annotation databases

EnsemblFungiiYAL058W; YAL058W; YAL058W
GeneIDi851241
KEGGisce:YAL058W

Similar proteinsi

Entry informationi

Entry nameiCALX_YEAST
AccessioniPrimary (citable) accession number: P27825
Secondary accession number(s): D6VPG1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: April 25, 2018
This is version 161 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health