Reviewed, UniProtKB/Swiss-Prot P27823 (PEPAF_RABIT)

Names and origin

Protein names	Recommended name:     Pepsin F     EC=3.4.23.1
Organism	Oryctolagus cuniculus (Rabbit)
Taxonomic identifier	9986 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent.
Catalytic activity	Preferential cleavage: hydrophobic, preferably aromatic, residues in P1 and P1' positions. Cleaves 1-Phe-|-Val-2, 4-Gln-|-His-5, 13-Glu-|-Ala-14, 14-Ala-|-Leu-15, 15-Leu-|-Tyr-16, 16-Tyr-|-Leu-17, 23-Gly-|-Phe-24, 24-Phe-|-Phe-25 and 25-Phe-|-Tyr-26 bonds in the B chain of insulin.
Subcellular location	Secreted.
Developmental stage	Early postnatal.
Sequence similarities	Belongs to the peptidase A1 family.

Ontologies

Keywords
Biological process	Digestion
Cellular component	Secreted
Domain	Signal
Molecular function	Aspartyl protease Hydrolase Protease
PTM	Disulfide bond Zymogen
Gene Ontology (GO)
Biological process	digestion Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 15	15
	Propeptide	16 – 58	43	Activation peptide		PRO_0000026036
	Chain	59 – 388	330	Pepsin F		PRO_0000026037
Sites
	Active site	92	1	By similarity
	Active site	275	1	By similarity
Amino acid modifications
	Disulfide bond	105 ↔ 110		By similarity
	Disulfide bond	266 ↔ 270		By similarity
	Disulfide bond	309 ↔ 343		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P27823-1 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 24792BE393594B3A	FASTA	388	42,786
	10 20 30 40 50 60 MKWLGLLGLV ALSECLVTIP LMKVKSMREN LRENDILLDY LEKHPYRPTY KLLSGQQDPD 70 80 90 100 110 120 VSFEPLRNYL DLAYIGIISI GTPPQEFKVV LDTGSADLWV PSIYCSSPAC GKHNTFNPLL 130 140 150 160 170 180 SSTFLVSGRP INIVYGSGRM SGFLAYDTVQ IAGLVDVAQA FGLSLQEPGK FMEYAVFDGI 190 200 210 220 230 240 LGLSYPSLSF EGITPVFDNL WAQGLISQNL FAFYLSSKEE RGSMLMLGGV DPSYYSGDLH 250 260 270 280 290 300 WVPVSRPLYW QLAVDRISMN GEAIGCDSGC QGIVDTGTSL LIGPRDPVLN IQKIINAQHS 310 320 330 340 350 360 HGGEYIIDCD TISTLPDIIF TIDGVDYPVP ASAYIRKSSV HGCYSNFDES AAHESEPYEV 370 380 WVLGDVFLRL YFTVFDRANN RIGLAPAV

References

[1]

"Structure and development of rabbit pepsinogens. Stage-specific zymogens, nucleotide sequences of cDNAs, molecular evolution, and gene expression during development." Kageyama T., Tanabe K., Koiwai O. J. Biol. Chem. 265:17031-17038(1990) [PubMed: 2129536] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Japanese white. Tissue: Gastric mucosa.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M59238 mRNA. Translation: AAA31440.1.
PIR	A38302.
RefSeq	NP_001076165.1.
UniGene	Ocu.1945
3D structure databases
HSSP	HSSP built from PDB template 4CMS based on UniProtKB P00794.
ModBase	Search...
Protein family/group databases
MEROPS	A01.051.
Genome annotation databases
GeneID	100009429.
Phylogenomic databases
HOVERGEN	P27823.
Enzyme and pathway databases
BRENDA	3.4.23.1. 255.
Family and domain databases
InterPro	IPR001461. Peptidase_A1. IPR001969. Peptidase_aspartic_AS. IPR009007. Peptidase_aspartic_catalytic. IPR012848. Propep_A1. [Graphical view]
Gene3D	G3DSA:2.40.70.10. Pept_Aspartc_cat. 1 hit.
PANTHER	PTHR13683. Peptidase_A1. 1 hit.
Pfam	PF07966. A1_Propeptide. 1 hit. PF00026. Asp. 1 hit. [Graphical view]
PRINTS	PR00792. PEPSIN.
PROSITE	PS00141. ASP_PROTEASE. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PEPAF_RABIT

Accession

Primary (citable) accession number: P27823

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	June 16, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)