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Protein

Pepsin F

Gene
N/A
Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent.

Catalytic activityi

Preferential cleavage: hydrophobic, preferably aromatic, residues in P1 and P1' positions. Cleaves 1-Phe-|-Val-2, 4-Gln-|-His-5, 13-Glu-|-Ala-14, 14-Ala-|-Leu-15, 15-Leu-|-Tyr-16, 16-Tyr-|-Leu-17, 23-Gly-|-Phe-24, 24-Phe-|-Phe-25 and 25-Phe-|-Tyr-26 bonds in the B chain of insulin.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei92 – 921PROSITE-ProRule annotation
Active sitei275 – 2751PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Hydrolase, Protease

Keywords - Biological processi

Digestion

Protein family/group databases

MEROPSiA01.051.

Names & Taxonomyi

Protein namesi
Recommended name:
Pepsin F (EC:3.4.23.1)
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
Proteomesi
  • UP000001811 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1515Add
BLAST
Propeptidei16 – 5843Activation peptidePRO_0000026036Add
BLAST
Chaini59 – 388330Pepsin FPRO_0000026037Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi105 ↔ 110By similarity
Disulfide bondi266 ↔ 270By similarity
Disulfide bondi309 ↔ 343By similarity

Keywords - PTMi

Disulfide bond, Zymogen

Expressioni

Developmental stagei

Early postnatal.

Interactioni

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000006601.

Structurei

3D structure databases

ProteinModelPortaliP27823.
SMRiP27823. Positions 64-388.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini74 – 385312Peptidase A1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase A1 family.Curated
Contains 1 peptidase A1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1339. Eukaryota.
ENOG410XNV7. LUCA.
HOGENOMiHOG000197681.
HOVERGENiHBG000482.
KOiK06002.

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR012848. Aspartic_peptidase_N.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PfamiPF07966. A1_Propeptide. 1 hit.
PF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27823-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKWLGLLGLV ALSECLVTIP LMKVKSMREN LRENDILLDY LEKHPYRPTY
60 70 80 90 100
KLLSGQQDPD VSFEPLRNYL DLAYIGIISI GTPPQEFKVV LDTGSADLWV
110 120 130 140 150
PSIYCSSPAC GKHNTFNPLL SSTFLVSGRP INIVYGSGRM SGFLAYDTVQ
160 170 180 190 200
IAGLVDVAQA FGLSLQEPGK FMEYAVFDGI LGLSYPSLSF EGITPVFDNL
210 220 230 240 250
WAQGLISQNL FAFYLSSKEE RGSMLMLGGV DPSYYSGDLH WVPVSRPLYW
260 270 280 290 300
QLAVDRISMN GEAIGCDSGC QGIVDTGTSL LIGPRDPVLN IQKIINAQHS
310 320 330 340 350
HGGEYIIDCD TISTLPDIIF TIDGVDYPVP ASAYIRKSSV HGCYSNFDES
360 370 380
AAHESEPYEV WVLGDVFLRL YFTVFDRANN RIGLAPAV
Length:388
Mass (Da):42,786
Last modified:August 1, 1992 - v1
Checksum:i24792BE393594B3A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59238 mRNA. Translation: AAA31440.1.
PIRiA38302.
RefSeqiNP_001076165.1. NM_001082696.1.
UniGeneiOcu.1945.

Genome annotation databases

GeneIDi100009429.
KEGGiocu:100009429.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59238 mRNA. Translation: AAA31440.1.
PIRiA38302.
RefSeqiNP_001076165.1. NM_001082696.1.
UniGeneiOcu.1945.

3D structure databases

ProteinModelPortaliP27823.
SMRiP27823. Positions 64-388.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000006601.

Protein family/group databases

MEROPSiA01.051.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100009429.
KEGGiocu:100009429.

Organism-specific databases

CTDi5222.

Phylogenomic databases

eggNOGiKOG1339. Eukaryota.
ENOG410XNV7. LUCA.
HOGENOMiHOG000197681.
HOVERGENiHBG000482.
KOiK06002.

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR012848. Aspartic_peptidase_N.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PfamiPF07966. A1_Propeptide. 1 hit.
PF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPEPAF_RABIT
AccessioniPrimary (citable) accession number: P27823
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: April 13, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.