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Protein

Pepsin II-2/3

Gene
N/A
Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent.

Catalytic activityi

Preferential cleavage: hydrophobic, preferably aromatic, residues in P1 and P1' positions. Cleaves 1-Phe-|-Val-2, 4-Gln-|-His-5, 13-Glu-|-Ala-14, 14-Ala-|-Leu-15, 15-Leu-|-Tyr-16, 16-Tyr-|-Leu-17, 23-Gly-|-Phe-24, 24-Phe-|-Phe-25 and 25-Phe-|-Tyr-26 bonds in the B chain of insulin.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei93PROSITE-ProRule annotation1
Active sitei276PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAspartyl protease, Hydrolase, Protease
Biological processDigestion

Protein family/group databases

MEROPSiA01.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Pepsin II-2/3 (EC:3.4.23.1)
Alternative name(s):
Pepsin A
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
Proteomesi
  • UP000001811 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 15Add BLAST15
PropeptideiPRO_000002603016 – 59Activation peptideAdd BLAST44
ChainiPRO_000002603160 – 387Pepsin II-2/3Add BLAST328

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi106 ↔ 111By similarity
Modified residuei129PhosphoserineBy similarity1
Disulfide bondi267 ↔ 271By similarity
Disulfide bondi310 ↔ 343By similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein, Zymogen

Expressioni

Developmental stagei

Pepsinogens in group I, II, and III where the predominant zymogens at late postnatal stage.

Interactioni

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000007186.

Structurei

3D structure databases

ProteinModelPortaliP27821.
SMRiP27821.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini75 – 384Peptidase A1PROSITE-ProRule annotationAdd BLAST310

Sequence similaritiesi

Belongs to the peptidase A1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1339. Eukaryota.
ENOG410XNV7. LUCA.
HOGENOMiHOG000197681.
HOVERGENiHBG000482.
KOiK06002.

Family and domain databases

CDDicd05478. pepsin_A. 1 hit.
Gene3Di2.40.70.10. 2 hits.
InterProiView protein in InterPro
IPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR012848. Aspartic_peptidase_N.
IPR034162. Pepsin_A.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
PANTHERiPTHR13683. PTHR13683. 1 hit.
PfamiView protein in Pfam
PF07966. A1_Propeptide. 1 hit.
PF00026. Asp. 1 hit.
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiView protein in PROSITE
PS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27821-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKWLLLLGLL ALSECIVHKV PLVRKKSLRK NLIEKGLLQD YLKTHTPNPA
60 70 80 90 100
TKYFPKETFA TVSTESMENY LDAEYFGTIS IGTPPQDFTV IFDTGSSNLW
110 120 130 140 150
VPSTYCSSLA CALHKRFNPE DSSTYQGTSE TLSITYGTGS MTGILGYDTV
160 170 180 190 200
KVGSIEDTNQ IFGLSKTEPS LTFLFAPFDG ILGLAYPSIS SSDATPVFDN
210 220 230 240 250
MWNEGLVSQD LFSVYLSSDD EKGSLVMFGG IDSSYYTGSL NWVPVSYEGY
260 270 280 290 300
WQITMDSVSI NGETIACADS CQAIVDTGTS LLTGPTSAIS NIQSYIGASK
310 320 330 340 350
NLLGENVISC SAIDSLPDIV FTINGIQYPL PASAYILKED DDCTSGLEGM
360 370 380
NVDTYTGELW ILGDVFIRQY FTVFDRANNQ LGLAAAV
Length:387
Mass (Da):42,100
Last modified:August 1, 1992 - v1
Checksum:i66FC331A3DC75891
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59235 mRNA. Translation: AAA85369.1.
PIRiC38302.
RefSeqiNP_001164557.1. NM_001171086.1.
UniGeneiOcu.2045.
Ocu.7471.

Genome annotation databases

GeneIDi100328625.
KEGGiocu:100328625.

Similar proteinsi

Entry informationi

Entry nameiPEPA2_RABIT
AccessioniPrimary (citable) accession number: P27821
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: March 15, 2017
This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families