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P27819 (ILVB3_BRANA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Acetolactate synthase 3, chloroplastic

EC=2.2.1.6
Alternative name(s):
ALS III
Acetohydroxy-acid synthase III
Acetolactate synthase III
OrganismBrassica napus (Rape)
Taxonomic identifier3708 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

Protein attributes

Sequence length652 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

2 pyruvate = 2-acetolactate + CO2.

Cofactor

Binds 1 magnesium ion per subunit By similarity.

Binds 1 thiamine pyrophosphate per subunit By similarity.

Pathway

Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4.

Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.

Subcellular location

Plastidchloroplast.

Miscellaneous

Acetolactate synthase is the target enzyme for sulfonylurea and imidazolinone herbicides.

Sequence similarities

Belongs to the TPP enzyme family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 6969Chloroplast By similarity
Chain70 – 652583Acetolactate synthase 3, chloroplastic
PRO_0000035658

Regions

Nucleotide binding334 – 35522FAD By similarity
Nucleotide binding377 – 39620FAD By similarity
Region469 – 54981Thiamine pyrophosphate binding

Sites

Metal binding5201Magnesium By similarity
Metal binding5471Magnesium By similarity
Binding site1261Thiamine pyrophosphate By similarity
Binding site2281FAD By similarity

Sequences

Sequence LengthMass (Da)Tools
P27819 [UniParc].

Last modified August 1, 1992. Version 1.
Checksum: B12534C50CC5AE0D

FASTA65270,988
        10         20         30         40         50         60 
MAAATSSSPI SLTAKPSSKS PLPISRFSLP FSLTPQKPSS RLHRPLAISA VLNSPVNVAP 

        70         80         90        100        110        120 
EKTDKIKTFI SRYAPDEPRK GADILVEALE RQGVETVFAY PGGASMEIHQ ALTRSSTIRN 

       130        140        150        160        170        180 
VLPRHEQGGV FAAEGYARSS GKPGICIATS GPGATNLVSG LADAMLDSVP LVAITGQVPR 

       190        200        210        220        230        240 
RMIGTDAFQE TPIVEVTRSI TKHNYLVMDV DDIPRIVQEA FFLATSGRPG PVLVDVPKDI 

       250        260        270        280        290        300 
QQQLAIPNWD QPMRLPGYMS RLPQPPEVSQ LGQIVRLISE SKRPVLYVGG GSLNSSEELG 

       310        320        330        340        350        360 
RFVELTGIPV ASTLMGLGSY PCNDELSLQM LGMHGTVYAN YAVEHSDLLL AFGVRFDDRV 

       370        380        390        400        410        420 
TGKLEAFASR AKIVHIDIDS AEIGKNKTPH VSVCGDVKLA LQGMNKVLEN RAEELKLDFG 

       430        440        450        460        470        480 
VWRSELSEQK QKFPLSFKTF GEAIPPQYAI QVLDELTQGK AIISTGVGQH QMWAAQFYKY 

       490        500        510        520        530        540 
RKPRQWLSSS GLGAMGFGLP AAIGASVANP DAIVVDIDGD GSFIMNVQEL ATIRVENLPV 

       550        560        570        580        590        600 
KILLLNNQHL GMVMQWEDRF YKANRAHTYL GDPARENEIF PNMLQFAGAC GIPAARVTKK 

       610        620        630        640        650 
EELREAIQTM LDTPGPYLLD VICPHQEHVL PMIPSGGTFK DVITEGDGRT KY 

« Hide

References

[1]"Molecular characterization and genetic origin of the Brassica napus acetohydroxyacid synthase multigene family."
Rutledge R.G., Ouellet T., Hattori J., Miki B.L.A.
Mol. Gen. Genet. 229:31-40(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Topas.
[2]Miki B.L.
Submitted (FEB-1992) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z11526 Genomic DNA. Translation: CAA77615.1.
PIRS29838.

3D structure databases

ProteinModelPortalP27819.
SMRP27819. Positions 68-649.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00047; UER00055.
UPA00049; UER00059.

Family and domain databases

Gene3D3.40.50.1220. 1 hit.
3.40.50.970. 2 hits.
InterProIPR012846. Acetolactate_synth_lsu.
IPR029035. DHS-like_NAD/FAD-binding_dom.
IPR029061. THDP-binding.
IPR012000. Thiamin_PyroP_enz_cen_dom.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
IPR000399. TPP-bd_CS.
IPR011766. TPP_enzyme-bd_C.
[Graphical view]
PfamPF02775. TPP_enzyme_C. 1 hit.
PF00205. TPP_enzyme_M. 1 hit.
PF02776. TPP_enzyme_N. 1 hit.
[Graphical view]
SUPFAMSSF52467. SSF52467. 1 hit.
SSF52518. SSF52518. 2 hits.
TIGRFAMsTIGR00118. acolac_lg. 1 hit.
PROSITEPS00187. TPP_ENZYMES. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameILVB3_BRANA
AccessionPrimary (citable) accession number: P27819
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: June 11, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways