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P27818

- ILVB1_BRANA

UniProt

P27818 - ILVB1_BRANA

Protein

Acetolactate synthase 1, chloroplastic

Gene
N/A
Organism
Brassica napus (Rape)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 1 (01 Aug 1992)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    2 pyruvate = 2-acetolactate + CO2.

    Cofactori

    Binds 1 magnesium ion per subunit.By similarity
    Binds 1 thiamine pyrophosphate per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei129 – 1291Thiamine pyrophosphateBy similarity
    Binding sitei231 – 2311FADBy similarity
    Metal bindingi523 – 5231MagnesiumBy similarity
    Metal bindingi550 – 5501MagnesiumBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi337 – 35822FADBy similarityAdd
    BLAST
    Nucleotide bindingi380 – 39920FADBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. acetolactate synthase activity Source: UniProtKB-EC
    2. flavin adenine dinucleotide binding Source: InterPro
    3. magnesium ion binding Source: InterPro
    4. thiamine pyrophosphate binding Source: InterPro

    GO - Biological processi

    1. isoleucine biosynthetic process Source: UniProtKB-UniPathway
    2. response to herbicide Source: UniProtKB-KW
    3. valine biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Herbicide resistance

    Keywords - Ligandi

    FAD, Flavoprotein, Magnesium, Metal-binding, Thiamine pyrophosphate

    Enzyme and pathway databases

    UniPathwayiUPA00047; UER00055.
    UPA00049; UER00059.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acetolactate synthase 1, chloroplastic (EC:2.2.1.6)
    Alternative name(s):
    ALS I
    Acetohydroxy-acid synthase I
    Acetolactate synthase I
    OrganismiBrassica napus (Rape)
    Taxonomic identifieri3708 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 8282ChloroplastBy similarityAdd
    BLAST
    Chaini83 – 655573Acetolactate synthase 1, chloroplasticPRO_0000035656Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP27818.
    SMRiP27818. Positions 71-652.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni472 – 55281Thiamine pyrophosphate bindingAdd
    BLAST

    Sequence similaritiesi

    Belongs to the TPP enzyme family.Curated

    Keywords - Domaini

    Transit peptide

    Family and domain databases

    Gene3Di3.40.50.1220. 1 hit.
    3.40.50.970. 2 hits.
    InterProiIPR012846. Acetolactate_synth_lsu.
    IPR029035. DHS-like_NAD/FAD-binding_dom.
    IPR029061. THDP-binding.
    IPR012000. Thiamin_PyroP_enz_cen_dom.
    IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
    IPR000399. TPP-bd_CS.
    IPR011766. TPP_enzyme-bd_C.
    [Graphical view]
    PfamiPF02775. TPP_enzyme_C. 1 hit.
    PF00205. TPP_enzyme_M. 1 hit.
    PF02776. TPP_enzyme_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF52467. SSF52467. 1 hit.
    SSF52518. SSF52518. 2 hits.
    TIGRFAMsiTIGR00118. acolac_lg. 1 hit.
    PROSITEiPS00187. TPP_ENZYMES. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P27818-1 [UniParc]FASTAAdd to Basket

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    MAAATSSSPI SLTAKPSSKS PLPISRFSLP FSLTPQKDSS RLHRPLAISA    50
    VLNSPVNVAP PSPEKTDKNK TFVSRYAPDE PRKGADILVE ALERQGVETV 100
    FAYPGGASME IHQALTRSST IRNVLPRHEQ GGVFAAEGYA RSSGKPGICI 150
    ATSGPGATNL VSGLADAMLD SVPLVAITGQ VPRRMIGTDA FQETPIVEVT 200
    RSITKHNYLV MDVDDIPRIV QEAFFLATSG RPGPVLVDVP KDIQQQLAIP 250
    NWDQPMRLPG YMSRLPQPPE VSQLGQIVRL ISESKRPVLY VGGGSLNSSE 300
    ELGRFVELTG IPVASTLMGL GSYPCNDELS LQMLGMHGTV YANYAVEHSD 350
    LLLAFGVRFD DRVTGKLEAF ASRAKIVHID IDSAEIGKNK TPHVSVCGDV 400
    KLALQGMNKV LENRAEELKL DFGVWRSELS EQKQKFPLSF KTFGEAIPPQ 450
    YAIQILDELT EGKAIISTGV GQHQMWAAQF YKYRKPRQWL SSSGLGAMGF 500
    GLPAAIGASV ANPDAIVVDI DGDGSFIMNV QELATIRVEN LPVKILLLNN 550
    QHLGMVMQWE DRFYKANRAH TYLGDPAREN EIFPNMLQFA GACGIPAARV 600
    TKKEELREAI QTMLDTPGPY LLDVICPHQE HVLPMIPSGG TFKDVITEGD 650
    GRTKY 655
    Length:655
    Mass (Da):71,289
    Last modified:August 1, 1992 - v1
    Checksum:i1BEAD7D7A0DAD91A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z11524 Genomic DNA. Translation: CAA77613.1.
    PIRiS17691.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z11524 Genomic DNA. Translation: CAA77613.1 .
    PIRi S17691.

    3D structure databases

    ProteinModelPortali P27818.
    SMRi P27818. Positions 71-652.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00047 ; UER00055 .
    UPA00049 ; UER00059 .

    Family and domain databases

    Gene3Di 3.40.50.1220. 1 hit.
    3.40.50.970. 2 hits.
    InterProi IPR012846. Acetolactate_synth_lsu.
    IPR029035. DHS-like_NAD/FAD-binding_dom.
    IPR029061. THDP-binding.
    IPR012000. Thiamin_PyroP_enz_cen_dom.
    IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
    IPR000399. TPP-bd_CS.
    IPR011766. TPP_enzyme-bd_C.
    [Graphical view ]
    Pfami PF02775. TPP_enzyme_C. 1 hit.
    PF00205. TPP_enzyme_M. 1 hit.
    PF02776. TPP_enzyme_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52467. SSF52467. 1 hit.
    SSF52518. SSF52518. 2 hits.
    TIGRFAMsi TIGR00118. acolac_lg. 1 hit.
    PROSITEi PS00187. TPP_ENZYMES. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterization and genetic origin of the Brassica napus acetohydroxyacid synthase multigene family."
      Rutledge R.G., Ouellet T., Hattori J., Miki B.L.A.
      Mol. Gen. Genet. 229:31-40(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cv. Topas.

    Entry informationi

    Entry nameiILVB1_BRANA
    AccessioniPrimary (citable) accession number: P27818
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: August 1, 1992
    Last modified: October 1, 2014
    This is version 95 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Acetolactate synthase is the target enzyme for sulfonylurea and imidazolinone herbicides.

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3