P27810 (KTR1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-1,2 mannosyltransferase KTR1
EC=2.4.1.-

Gene names

Name:	KTR1
Ordered Locus Names:	YOR099W
ORF Names:	YOR3189W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	393 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mannosyltransferase that transfers a mannose residue from GDP-mannose to a range of acceptors in vitro.
Catalytic activity	Transfers an alpha-D-mannosyl residue from GDP-mannose into lipid-linked oligosaccharide, forming an alpha-(1->2)-D-mannosyl-D-mannose linkage.
Cofactor	Manganese.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein.
Post-translational modification	N-glycosylated. Ref.7
Miscellaneous	Present with 5480 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the glycosyltransferase 15 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Manganese
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell wall mannoprotein biosynthetic process Inferred from mutant phenotype. Source: SGD protein glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alpha-1,2-mannosyltransferase activity Inferred from direct assay Ref.5. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 393	393	Alpha-1,2 mannosyltransferase KTR1		PRO_0000208242
Regions
	Topological domain	1 – 16	16	Cytoplasmic Potential
	Transmembrane	17 – 34	18	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	35 – 393	359	Lumenal Potential
	Region	35 – 68	34	Stem region By similarity
	Region	69 – 393	325	Catalytic By similarity
Sites
	Active site	280	1	Nucleophile Potential
Amino acid modifications
	Glycosylation	120	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P27810 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: F93DDF2953EA287B	FASTA	393	46,022
	10 20 30 40 50 60 MAKIMIPASK QPVYKKLGLL LVAVFTVYVF FHGAQYARGS APSPKYSTVL SSGSGYKYSK 70 80 90 100 110 120 VELPKYTGPR EKATFVTLVR NRDLYSLAES IKSVEDRFNS KFNYDWVFLN DEEFTDEFKN 130 140 150 160 170 180 VTSALVSGTT KYGVIPKEHW SFPEWIDEEK AAQVRKEMGE KRIIYGDSIS YRHMCRFESG 190 200 210 220 230 240 FFYRHPLMDD YDWYWRVEPD IKLHCDIDYD VFKFMKDNKK KYAFAISIKE YEATIPTLWE 250 260 270 280 290 300 TTRKFMEAHP ELIHENNMLD FVSDDQGLSY NLCHFWSNFE IAALDLWRSP AYSAYFDYLD 310 320 330 340 350 360 REGGFFYERW GDAPVHSIGA ALFLDRSEIH HFGDIGYYHV PFHSCPIDTS IRLANKCDCD 370 380 390 PSKDFTWHSY SCTTKFYNIN KLPKPAGWQN HIG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Yeast KRE2 defines a new gene family encoding probable secretory proteins, and is required for the correct N-glycosylation of proteins." Hill K., Boone C., Goebl M., Puccia R., Sdicu A.-M., Bussey H. Genetics 130:273-283(1992) [PubMed: 1541391] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"DNA sequencing and analysis of 130 kb from yeast chromosome XV." Voss H., Benes V., Andrade M.A., Valencia A., Rechmann S., Teodoru C., Schwager C., Paces V., Sander C., Ansorge W. Yeast 13:655-672(1997) [PubMed: 9200815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Ktr1p is an alpha-1,2-mannosyltransferase of Saccharomyces cerevisiae. Comparison of the enzymic properties of soluble recombinant Ktr1p and Kre2p/Mnt1p produced in Pichia pastoris." Romero P.A., Lussier M., Sdicu A.-M., Bussey H. Biochem. J. 321:289-295(1997) [PubMed: 9020857] [Abstract] Cited for: CHARACTERIZATION.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of the glycoproteome in Saccharomyces cerevisiae reveals new roles for protein glycosylation in eukaryotes." Kung L.A., Tao S.-C., Qian J., Smith M.G., Snyder M., Zhu H. Mol. Syst. Biol. 5:308-308(2009) [PubMed: 19756047] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X62941 Genomic DNA. Translation: CAA44713.1. X94335 Genomic DNA. Translation: CAA64021.1. Z75007 Genomic DNA. Translation: CAA99296.1. BK006948 Genomic DNA. Translation: DAA10876.1.
PIR	S61659.
RefSeq	NP_014742.1. NM_001183518.1.
3D structure databases
ProteinModelPortal	P27810.
SMR	P27810. Positions 63-393.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-7181N.
IntAct	P27810. 1 interaction.
STRING	P27810.
Protein family/group databases
CAZy	GT15. Glycosyltransferase Family 15.
Proteomic databases
PeptideAtlas	P27810.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YOR099W; YOR099W; YOR099W.
GeneID	854266.
KEGG	sce:YOR099W.
NMPDR	fig|4932.3.peg.5844.
Organism-specific databases
CYGD	YOR099w.
SGD	S000005625. KTR1.
Phylogenomic databases
eggNOG	fuNOG04371.
GeneTree	EFGT00050000003570.
HOGENOM	HBG396033.
OMA	EYKETIP.
OrthoDB	EOG428598.
Gene expression databases
ArrayExpress	P27810.
Genevestigator	P27810.
GermOnline	YOR099W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002685. Glyco_trans_15. [Graphical view]
KO	K10967.
Pfam	PF01793. Glyco_transf_15. 1 hit. [Graphical view]
PIRSF	PIRSF018153. Glyco_trans_15. 1 hit.
ProtoNet	Search...
Other
NextBio	976212.

Entry information

Entry name

KTR1_YEAST

Accession

Primary (citable) accession number: P27810
Secondary accession number(s): D6W2G0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	December 14, 2011
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program