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Protein

Glycolipid 2-alpha-mannosyltransferase

Gene

KRE2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for the attachment of the third mannose residue of O-linked saccharides.

Catalytic activityi

Transfers an alpha-D-mannosyl residue from GDP-mannose into lipid-linked oligosaccharide, forming an alpha-(1->2)-D-mannosyl-D-mannose linkage.

Cofactori

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei329NucleophileSequence analysis1

GO - Molecular functioni

  • alpha-1,2-mannosyltransferase activity Source: SGD

GO - Biological processi

  • cell wall mannoprotein biosynthetic process Source: SGD
  • N-glycan processing Source: SGD
  • protein O-linked glycosylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese

Enzyme and pathway databases

BioCyciYEAST:G3O-30008-MONOMER.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT15. Glycosyltransferase Family 15.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycolipid 2-alpha-mannosyltransferase (EC:2.4.1.-)
Alternative name(s):
Alpha-1,2-mannosyltransferase
Gene namesi
Name:KRE2
Synonyms:MNT1
Ordered Locus Names:YDR483W
ORF Names:D8035.26
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR483W.
SGDiS000002891. KRE2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 11CytoplasmicSequence analysisAdd BLAST11
Transmembranei12 – 30Helical; Signal-anchor for type II membrane proteinAdd BLAST19
Topological domaini31 – 442LumenalSequence analysisAdd BLAST412

GO - Cellular componenti

  • Golgi apparatus Source: SGD
  • Golgi membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002082411 – 442Glycolipid 2-alpha-mannosyltransferaseAdd BLAST442

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi197N-linked (GlcNAc...)1 Publication1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP27809.
PRIDEiP27809.

Interactioni

Protein-protein interaction databases

BioGridi32535. 26 interactors.
DIPiDIP-5373N.
IntActiP27809. 11 interactors.
MINTiMINT-514227.

Structurei

Secondary structure

1442
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi106 – 110Combined sources5
Helixi111 – 113Combined sources3
Beta strandi123 – 128Combined sources6
Helixi131 – 133Combined sources3
Helixi134 – 147Combined sources14
Turni148 – 151Combined sources4
Beta strandi156 – 162Combined sources7
Helixi166 – 175Combined sources10
Beta strandi180 – 184Combined sources5
Helixi187 – 189Combined sources3
Helixi198 – 208Combined sources11
Turni209 – 211Combined sources3
Turni213 – 216Combined sources4
Helixi218 – 229Combined sources12
Helixi231 – 233Combined sources3
Helixi235 – 237Combined sources3
Beta strandi241 – 245Combined sources5
Helixi260 – 266Combined sources7
Beta strandi271 – 274Combined sources4
Beta strandi276 – 278Combined sources3
Helixi281 – 283Combined sources3
Helixi287 – 297Combined sources11
Helixi299 – 301Combined sources3
Helixi308 – 311Combined sources4
Beta strandi322 – 324Combined sources3
Beta strandi328 – 332Combined sources5
Helixi333 – 336Combined sources4
Helixi339 – 351Combined sources13
Helixi353 – 356Combined sources4
Helixi361 – 372Combined sources12
Helixi375 – 377Combined sources3
Beta strandi378 – 380Combined sources3
Beta strandi386 – 388Combined sources3
Beta strandi391 – 393Combined sources3
Helixi398 – 403Combined sources6
Helixi410 – 412Combined sources3
Helixi422 – 429Combined sources8
Helixi437 – 441Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1S4NX-ray2.01A/B97-442[»]
1S4OX-ray2.01A/B97-442[»]
1S4PX-ray2.01A/B97-442[»]
ProteinModelPortaliP27809.
SMRiP27809.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP27809.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni31 – 118Stem regionAdd BLAST88
Regioni119 – 442CatalyticAdd BLAST324

Sequence similaritiesi

Belongs to the glycosyltransferase 15 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000000831.
HOGENOMiHOG000160667.
InParanoidiP27809.
KOiK10967.
OMAiHFHCDID.
OrthoDBiEOG092C3H4I.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR002685. Glyco_trans_15.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR31121. PTHR31121. 1 hit.
PfamiPF01793. Glyco_transf_15. 1 hit.
[Graphical view]
PIRSFiPIRSF018153. Glyco_trans_15. 1 hit.
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

P27809-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALFLSKRLL RFTVIAGAVI VLLLTLNSNS RTQQYIPSSI SAAFDFTSGS
60 70 80 90 100
ISPEQQVISE ENDAKKLEQS ALNSEASEDS EAMDEESKAL KAAAEKADAP
110 120 130 140 150
IDTKTTMDYI TPSFANKAGK PKACYVTLVR NKELKGLLSS IKYVENKINK
160 170 180 190 200
KFPYPWVFLN DEPFTEEFKE AVTKAVSSEV KFGILPKEHW SYPEWINQTK
210 220 230 240 250
AAEIRADAAT KYIYGGSESY RHMCRYQSGF FWRHELLEEY DWYWRVEPDI
260 270 280 290 300
KLYCDINYDV FKWMQENEKV YGFTVSIHEY EVTIPTLWQT SMDFIKKNPE
310 320 330 340 350
YLDENNLMSF LSNDNGKTYN LCHFWSNFEI ANLNLWRSPA YREYFDTLDH
360 370 380 390 400
QGGFFYERWG DAPVHSIAAA LFLPKDKIHY FSDIGYHHPP YDNCPLDKEV
410 420 430 440
YNSNNCECDQ GNDFTFQGYS CGKEYYDAQG LVKPKNWKKF RE
Length:442
Mass (Da):51,387
Last modified:August 1, 1992 - v1
Checksum:iE2A54786F8C8AA60
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M81110 Genomic DNA. Translation: AAA34784.1.
X62647 Genomic DNA. Translation: CAA44516.1.
U33050 Genomic DNA. Translation: AAB64906.1.
Z29988 Genomic DNA. Translation: CAA82879.1.
BK006938 Genomic DNA. Translation: DAA12316.1.
PIRiS32592.
RefSeqiNP_010771.1. NM_001180791.1.

Genome annotation databases

EnsemblFungiiYDR483W; YDR483W; YDR483W.
GeneIDi852094.
KEGGisce:YDR483W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M81110 Genomic DNA. Translation: AAA34784.1.
X62647 Genomic DNA. Translation: CAA44516.1.
U33050 Genomic DNA. Translation: AAB64906.1.
Z29988 Genomic DNA. Translation: CAA82879.1.
BK006938 Genomic DNA. Translation: DAA12316.1.
PIRiS32592.
RefSeqiNP_010771.1. NM_001180791.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1S4NX-ray2.01A/B97-442[»]
1S4OX-ray2.01A/B97-442[»]
1S4PX-ray2.01A/B97-442[»]
ProteinModelPortaliP27809.
SMRiP27809.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32535. 26 interactors.
DIPiDIP-5373N.
IntActiP27809. 11 interactors.
MINTiMINT-514227.

Protein family/group databases

CAZyiGT15. Glycosyltransferase Family 15.

Proteomic databases

MaxQBiP27809.
PRIDEiP27809.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR483W; YDR483W; YDR483W.
GeneIDi852094.
KEGGisce:YDR483W.

Organism-specific databases

EuPathDBiFungiDB:YDR483W.
SGDiS000002891. KRE2.

Phylogenomic databases

GeneTreeiENSGT00390000000831.
HOGENOMiHOG000160667.
InParanoidiP27809.
KOiK10967.
OMAiHFHCDID.
OrthoDBiEOG092C3H4I.

Enzyme and pathway databases

UniPathwayiUPA00378.
BioCyciYEAST:G3O-30008-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP27809.
PROiP27809.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR002685. Glyco_trans_15.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR31121. PTHR31121. 1 hit.
PfamiPF01793. Glyco_transf_15. 1 hit.
[Graphical view]
PIRSFiPIRSF018153. Glyco_trans_15. 1 hit.
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKRE2_YEAST
AccessioniPrimary (citable) accession number: P27809
Secondary accession number(s): D6VTA6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: November 2, 2016
This is version 160 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 13000 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.