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Reviewed, UniProtKB/Swiss-Prot P27809 (KRE2_YEAST)

Last modified June 16, 2009. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Glycolipid 2-alpha-mannosyltransferase
    EC=2.4.1.131
Alternative name(s):
    Alpha-1,2-mannosyltransferase
Gene names
Name: KRE2
Synonyms: MNT1
Ordered Locus Names: YDR483W
ORF Names: D8035.26
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length442 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Required for the attachment of the third mannose residue of O-linked saccharides.

Catalytic activity

Transfers an alpha-D-mannosyl residue from GDP-mannose into lipid-linked oligosaccharide, forming an alpha-(1->2)-D-mannosyl-D-mannose linkage.

Cofactor

Manganese.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Golgi apparatus membrane; Single-pass type II membrane protein.

Miscellaneous

Present with 13000 molecules/cell in log phase SD medium. Ref.5

Sequence similarities

Belongs to the glycosyltransferase 15 family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 442442Glycolipid 2-alpha-mannosyltransferase
PRO_0000208241

Regions

Topological domain1 – 1111Cytoplasmic Potential
Transmembrane12 – 3019Signal-anchor for type II membrane protein
Topological domain31 – 442412Lumenal Potential
Region31 – 11888Stem region
Region119 – 442324Catalytic

Sites

Active site3291Nucleophile Potential

Amino acid modifications

Glycosylation1971N-linked (GlcNAc...) Ref.1

Secondary structure

1 ....................................................................... 442
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
P27809-1 [UniParc].

Last modified August 1, 1992. Version 1.
Checksum: E2A54786F8C8AA60

FASTA44251,387
        10         20         30         40         50         60 
MALFLSKRLL RFTVIAGAVI VLLLTLNSNS RTQQYIPSSI SAAFDFTSGS ISPEQQVISE 

        70         80         90        100        110        120 
ENDAKKLEQS ALNSEASEDS EAMDEESKAL KAAAEKADAP IDTKTTMDYI TPSFANKAGK 

       130        140        150        160        170        180 
PKACYVTLVR NKELKGLLSS IKYVENKINK KFPYPWVFLN DEPFTEEFKE AVTKAVSSEV 

       190        200        210        220        230        240 
KFGILPKEHW SYPEWINQTK AAEIRADAAT KYIYGGSESY RHMCRYQSGF FWRHELLEEY 

       250        260        270        280        290        300 
DWYWRVEPDI KLYCDINYDV FKWMQENEKV YGFTVSIHEY EVTIPTLWQT SMDFIKKNPE 

       310        320        330        340        350        360 
YLDENNLMSF LSNDNGKTYN LCHFWSNFEI ANLNLWRSPA YREYFDTLDH QGGFFYERWG 

       370        380        390        400        410        420 
DAPVHSIAAA LFLPKDKIHY FSDIGYHHPP YDNCPLDKEV YNSNNCECDQ GNDFTFQGYS 

       430        440 
CGKEYYDAQG LVKPKNWKKF RE

« Hide

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References

« Hide 'large scale' references
[1]"Glycosylation in Saccharomyces cerevisiae: cloning and characterization of an alpha-1,2-mannosyltransferase structural gene."
Haeusler A., Robbins P.W.
Glycobiology 2:77-84(1992) [PubMed: 1550992] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, GLYCOSYLATION AT ASN-197.
[2]"Yeast KRE2 defines a new gene family encoding probable secretory proteins, and is required for the correct N-glycosylation of proteins."
Hill K., Boone C., Goebl M., Puccia R., Sdicu A.-M., Bussey H.
Genetics 130:273-283(1992) [PubMed: 1541391] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed: 9169867] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Nucleotide sequence of the SAC2 gene of Saccharomyces cerevisiae."
Koelling R., Lee A., Chen E.Y., Botstein D.
Yeast 10:1211-1216(1994) [PubMed: 7754710] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 101-442.
Strain: ATCC 204508 / S288c.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

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Cross-references

Sequence databases

M81110 Genomic DNA. Translation: AAA34784.1.
X62647 Genomic DNA. Translation: CAA44516.1.
U33050 Genomic DNA. Translation: AAB64906.1.
Z29988 Genomic DNA. Translation: CAA82879.1.
PIRS32592.
RefSeqNP_010771.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1S4NX-ray2.01A/B97-442[»]
1S4OX-ray2.01A/B97-442[»]
1S4PX-ray2.01A/B97-442[»]
ModBaseSearch...

Protein-protein interaction databases

DIPDIP:5373N.
IntActP27809. 16 interactions.

Protein family/group databases

CAZyGT15. Glycosyltransferase Family 15.

Proteomic databases

PeptideAtlasP27809.

Genome annotation databases

EnsemblYDR483W. Saccharomyces cerevisiae. [Contig view]
GeneID852094.
GenomeReviewsGene locus YDR483W in contig Z71256_GR.
KEGGsce:YDR483W.
NMPDRfig|4932.3.peg.1544.

Organism-specific databases

CYGDYDR483w.
SGDS000002891. KRE2.
Yeast-GFPSearch...

Phylogenomic databases

HOGENOMP27809.
OMAP27809. ETYNLCH.

Enzyme and pathway databases

BRENDA2.4.1.131. 250.

Gene expression databases

ArrayExpressP27809.
GermOnlineYDR483W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR002685. Glyco_trans_15.
[Graphical view]
PfamPF01793. Glyco_transf_15. 1 hit.
[Graphical view]
PIRSFPIRSF018153. Glyco_trans_15. 1 hit.
ProtoNetSearch...

Other Resources

NextBio970427.

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Entry information

Entry nameKRE2_YEAST
AccessionPrimary (citable) accession number: P27809
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: June 16, 2009
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents