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P27808 (MGAT1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase

EC=2.4.1.101
Alternative name(s):
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I
Short name=GNT-I
Short name=GlcNAc-T I
Gene names
Name:Mgat1
Synonyms:Gnt1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length447 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Initiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans.

Catalytic activity

UDP-N-acetyl-D-glucosamine + 3-(alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 3-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.

Cofactor

Manganese. The cofactor is mostly bound to the substrate By similarity.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Golgi apparatus membrane; Single-pass type II membrane protein.

Tissue specificity

Appears to be present in all tissues.

Sequence similarities

Belongs to the glycosyltransferase 13 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 447447Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
PRO_0000191385

Regions

Topological domain1 – 66Cytoplasmic Potential
Transmembrane7 – 2923Helical; Signal-anchor for type II membrane protein; Potential
Topological domain30 – 447418Lumenal Potential

Sites

Active site2911Proton acceptor Potential
Metal binding2131Manganese By similarity
Binding site1171Substrate By similarity
Binding site1441Substrate By similarity
Binding site1901Substrate By similarity
Binding site2121Substrate By similarity
Binding site3221Substrate By similarity

Amino acid modifications

Disulfide bond115 ↔ 145 By similarity
Disulfide bond239 ↔ 305 By similarity

Sequences

Sequence LengthMass (Da)Tools
P27808 [UniParc].

Last modified August 1, 1992. Version 1.
Checksum: E7C65EA8C8B34E6A

FASTA44751,690
        10         20         30         40         50         60 
MLKKQTAGLV LWGAIIFVGW NALLLLFFWT RPAPGRLPSD SALGDDPASL TREVIHLAED 

        70         80         90        100        110        120 
AEAELERQRG LLQQIKEHYA LWRQRWRVPT VAPPAWPRVP VTPSPVQIPI LVIACDRSTV 

       130        140        150        160        170        180 
RRCLDKLLHY RPSAERFPII VSQDCGHEET AQVIASYGTA VTHIRQPDLS NIAVQPDHRK 

       190        200        210        220        230        240 
FQGYYKIARH YRWALGQIFN KFKFPAAVVV EDDLEVAPDF FEYFQATYPL LRTDPSLWCV 

       250        260        270        280        290        300 
SAWNDNGKEQ MVDSSKPELL YRTDFFPGLG WLLLADLWAE LEPKWPKAFW DDWMRRPEQR 

       310        320        330        340        350        360 
KGRACIRPEI SRTMTFGRKG VSHGQFFDQH LKFIKLNQQF VPFTQLDLSY LQQEAYDRDF 

       370        380        390        400        410        420 
LAQVYGAPQL QVEKVRTNDQ KELGEVRVQY TSRDSFKAFA KALGVMDDLK SGVPRAGYRG 

       430        440 
IVTFQFRGRR VHLAPPQTWT GYDPSWN 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and characterization of the mouse UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I gene."
Pownall S., Kozak C.A., Schappert K.T., Sarkar M., Hull E., Schachter H., Math J.D.
Genomics 12:699-704(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Cloning and expression of the murine gene and chromosomal location of the human gene encoding N-acetylglucosaminyltransferase I."
Kumar R., Yang J., Eddy R.L., Byers M.G., Shows T.B., Stanley P.
Glycobiology 2:383-393(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: NOD.
Tissue: Thymus.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary gland.
+Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - GTase

alpha-1,3-mannosyl-glycoprotein beta 1,2-GlcNAc T I (Mgat1)

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M73491 Genomic DNA. Translation: AAA37698.1.
L07037 mRNA. Translation: AAA40478.1.
AK087959 mRNA. Translation: BAC40058.1.
BC006629 mRNA. Translation: AAH06629.1.
CCDSCCDS24602.1.
PIRA42500.
RefSeqNP_001103618.1. NM_001110148.1.
NP_001103619.1. NM_001110149.1.
NP_001103620.1. NM_001110150.1.
NP_034924.3. NM_010794.3.
XP_006532460.1. XM_006532397.1.
XP_006532461.1. XM_006532398.1.
XP_006532462.1. XM_006532399.1.
XP_006532463.1. XM_006532400.1.
XP_006532464.1. XM_006532401.1.
UniGeneMm.196933.

3D structure databases

ProteinModelPortalP27808.
SMRP27808. Positions 108-446.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-1836159.

Protein family/group databases

CAZyGT13. Glycosyltransferase Family 13.

PTM databases

PhosphoSiteP27808.

Proteomic databases

MaxQBP27808.
PaxDbP27808.
PRIDEP27808.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000081794; ENSMUSP00000080484; ENSMUSG00000020346.
ENSMUST00000101293; ENSMUSP00000098851; ENSMUSG00000020346.
ENSMUST00000109194; ENSMUSP00000104817; ENSMUSG00000020346.
ENSMUST00000167400; ENSMUSP00000126303; ENSMUSG00000020346.
GeneID17308.
KEGGmmu:17308.
UCSCuc007iqb.2. mouse.

Organism-specific databases

CTD4245.
MGIMGI:96973. Mgat1.

Phylogenomic databases

eggNOGNOG148227.
HOGENOMHOG000247055.
HOVERGENHBG052466.
InParanoidP27808.
KOK00726.
OMAHEETAQV.
OrthoDBEOG7R56SN.
PhylomeDBP27808.
TreeFamTF320555.

Enzyme and pathway databases

UniPathwayUPA00378.

Gene expression databases

ArrayExpressP27808.
BgeeP27808.
CleanExMM_MGAT1.
GenevestigatorP27808.

Family and domain databases

Gene3D3.90.550.10. 1 hit.
InterProIPR004139. Glyco_trans_13.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERPTHR10468. PTHR10468. 1 hit.
PfamPF03071. GNT-I. 1 hit.
[Graphical view]
SUPFAMSSF53448. SSF53448. 1 hit.
ProtoNetSearch...

Other

ChiTaRSMGAT1. mouse.
NextBio291856.
PROP27808.
SOURCESearch...

Entry information

Entry nameMGAT1_MOUSE
AccessionPrimary (citable) accession number: P27808
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: July 9, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot