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P27808

- MGAT1_MOUSE

UniProt

P27808 - MGAT1_MOUSE

Protein

Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase

Gene

Mgat1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 129 (01 Oct 2014)
      Sequence version 1 (01 Aug 1992)
      Previous versions | rss
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    Functioni

    Initiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans.

    Catalytic activityi

    UDP-N-acetyl-D-glucosamine + 3-(alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 3-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.

    Cofactori

    Manganese. The cofactor is mostly bound to the substrate By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei117 – 1171SubstrateBy similarity
    Binding sitei144 – 1441SubstrateBy similarity
    Binding sitei190 – 1901SubstrateBy similarity
    Binding sitei212 – 2121SubstrateBy similarity
    Metal bindingi213 – 2131ManganeseBy similarity
    Active sitei291 – 2911Proton acceptorSequence Analysis
    Binding sitei322 – 3221SubstrateBy similarity

    GO - Molecular functioni

    1. acetylglucosaminyltransferase activity Source: MGI
    2. alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity Source: UniProtKB-EC
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. in utero embryonic development Source: MGI
    2. protein glycosylation Source: UniProtKB-UniPathway
    3. UDP-N-acetylglucosamine catabolic process Source: MGI

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_198544. N-glycan trimming and elongation in the cis-Golgi.
    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT13. Glycosyltransferase Family 13.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (EC:2.4.1.101)
    Alternative name(s):
    N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I
    Short name:
    GNT-I
    Short name:
    GlcNAc-T I
    Gene namesi
    Name:Mgat1
    Synonyms:Gnt1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:96973. Mgat1.

    Subcellular locationi

    GO - Cellular componenti

    1. Golgi membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 447447Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferasePRO_0000191385Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi115 ↔ 145By similarity
    Disulfide bondi239 ↔ 305By similarity

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    MaxQBiP27808.
    PaxDbiP27808.
    PRIDEiP27808.

    PTM databases

    PhosphoSiteiP27808.

    Expressioni

    Tissue specificityi

    Appears to be present in all tissues.

    Gene expression databases

    ArrayExpressiP27808.
    BgeeiP27808.
    CleanExiMM_MGAT1.
    GenevestigatoriP27808.

    Interactioni

    Protein-protein interaction databases

    MINTiMINT-1836159.

    Structurei

    3D structure databases

    ProteinModelPortaliP27808.
    SMRiP27808. Positions 108-446.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 66CytoplasmicSequence Analysis
    Topological domaini30 – 447418LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei7 – 2923Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyltransferase 13 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG148227.
    HOGENOMiHOG000247055.
    HOVERGENiHBG052466.
    InParanoidiP27808.
    KOiK00726.
    OMAiHEETAQV.
    OrthoDBiEOG7R56SN.
    PhylomeDBiP27808.
    TreeFamiTF320555.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR004139. Glyco_trans_13.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PANTHERiPTHR10468. PTHR10468. 1 hit.
    PfamiPF03071. GNT-I. 1 hit.
    [Graphical view]
    SUPFAMiSSF53448. SSF53448. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P27808-1 [UniParc]FASTAAdd to Basket

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    MLKKQTAGLV LWGAIIFVGW NALLLLFFWT RPAPGRLPSD SALGDDPASL    50
    TREVIHLAED AEAELERQRG LLQQIKEHYA LWRQRWRVPT VAPPAWPRVP 100
    VTPSPVQIPI LVIACDRSTV RRCLDKLLHY RPSAERFPII VSQDCGHEET 150
    AQVIASYGTA VTHIRQPDLS NIAVQPDHRK FQGYYKIARH YRWALGQIFN 200
    KFKFPAAVVV EDDLEVAPDF FEYFQATYPL LRTDPSLWCV SAWNDNGKEQ 250
    MVDSSKPELL YRTDFFPGLG WLLLADLWAE LEPKWPKAFW DDWMRRPEQR 300
    KGRACIRPEI SRTMTFGRKG VSHGQFFDQH LKFIKLNQQF VPFTQLDLSY 350
    LQQEAYDRDF LAQVYGAPQL QVEKVRTNDQ KELGEVRVQY TSRDSFKAFA 400
    KALGVMDDLK SGVPRAGYRG IVTFQFRGRR VHLAPPQTWT GYDPSWN 447
    Length:447
    Mass (Da):51,690
    Last modified:August 1, 1992 - v1
    Checksum:iE7C65EA8C8B34E6A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M73491 Genomic DNA. Translation: AAA37698.1.
    L07037 mRNA. Translation: AAA40478.1.
    AK087959 mRNA. Translation: BAC40058.1.
    BC006629 mRNA. Translation: AAH06629.1.
    CCDSiCCDS24602.1.
    PIRiA42500.
    RefSeqiNP_001103618.1. NM_001110148.1.
    NP_001103619.1. NM_001110149.1.
    NP_001103620.1. NM_001110150.1.
    NP_034924.3. NM_010794.3.
    XP_006532460.1. XM_006532397.1.
    XP_006532461.1. XM_006532398.1.
    XP_006532462.1. XM_006532399.1.
    XP_006532463.1. XM_006532400.1.
    XP_006532464.1. XM_006532401.1.
    UniGeneiMm.196933.

    Genome annotation databases

    EnsembliENSMUST00000081794; ENSMUSP00000080484; ENSMUSG00000020346.
    ENSMUST00000101293; ENSMUSP00000098851; ENSMUSG00000020346.
    ENSMUST00000109194; ENSMUSP00000104817; ENSMUSG00000020346.
    ENSMUST00000167400; ENSMUSP00000126303; ENSMUSG00000020346.
    GeneIDi17308.
    KEGGimmu:17308.
    UCSCiuc007iqb.2. mouse.

    Cross-referencesi

    Web resourcesi

    Functional Glycomics Gateway - GTase

    alpha-1,3-mannosyl-glycoprotein beta 1,2-GlcNAc T I (Mgat1)

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M73491 Genomic DNA. Translation: AAA37698.1 .
    L07037 mRNA. Translation: AAA40478.1 .
    AK087959 mRNA. Translation: BAC40058.1 .
    BC006629 mRNA. Translation: AAH06629.1 .
    CCDSi CCDS24602.1.
    PIRi A42500.
    RefSeqi NP_001103618.1. NM_001110148.1.
    NP_001103619.1. NM_001110149.1.
    NP_001103620.1. NM_001110150.1.
    NP_034924.3. NM_010794.3.
    XP_006532460.1. XM_006532397.1.
    XP_006532461.1. XM_006532398.1.
    XP_006532462.1. XM_006532399.1.
    XP_006532463.1. XM_006532400.1.
    XP_006532464.1. XM_006532401.1.
    UniGenei Mm.196933.

    3D structure databases

    ProteinModelPortali P27808.
    SMRi P27808. Positions 108-446.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-1836159.

    Protein family/group databases

    CAZyi GT13. Glycosyltransferase Family 13.

    PTM databases

    PhosphoSitei P27808.

    Proteomic databases

    MaxQBi P27808.
    PaxDbi P27808.
    PRIDEi P27808.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000081794 ; ENSMUSP00000080484 ; ENSMUSG00000020346 .
    ENSMUST00000101293 ; ENSMUSP00000098851 ; ENSMUSG00000020346 .
    ENSMUST00000109194 ; ENSMUSP00000104817 ; ENSMUSG00000020346 .
    ENSMUST00000167400 ; ENSMUSP00000126303 ; ENSMUSG00000020346 .
    GeneIDi 17308.
    KEGGi mmu:17308.
    UCSCi uc007iqb.2. mouse.

    Organism-specific databases

    CTDi 4245.
    MGIi MGI:96973. Mgat1.

    Phylogenomic databases

    eggNOGi NOG148227.
    HOGENOMi HOG000247055.
    HOVERGENi HBG052466.
    InParanoidi P27808.
    KOi K00726.
    OMAi HEETAQV.
    OrthoDBi EOG7R56SN.
    PhylomeDBi P27808.
    TreeFami TF320555.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    Reactomei REACT_198544. N-glycan trimming and elongation in the cis-Golgi.

    Miscellaneous databases

    ChiTaRSi MGAT1. mouse.
    NextBioi 291856.
    PROi P27808.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P27808.
    Bgeei P27808.
    CleanExi MM_MGAT1.
    Genevestigatori P27808.

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    InterProi IPR004139. Glyco_trans_13.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view ]
    PANTHERi PTHR10468. PTHR10468. 1 hit.
    Pfami PF03071. GNT-I. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53448. SSF53448. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and characterization of the mouse UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I gene."
      Pownall S., Kozak C.A., Schappert K.T., Sarkar M., Hull E., Schachter H., Math J.D.
      Genomics 12:699-704(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Cloning and expression of the murine gene and chromosomal location of the human gene encoding N-acetylglucosaminyltransferase I."
      Kumar R., Yang J., Eddy R.L., Byers M.G., Shows T.B., Stanley P.
      Glycobiology 2:383-393(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: NOD.
      Tissue: Thymus.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary gland.

    Entry informationi

    Entry nameiMGAT1_MOUSE
    AccessioniPrimary (citable) accession number: P27808
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: August 1, 1992
    Last modified: October 1, 2014
    This is version 129 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3