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Protein

Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase

Gene

Mgat1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Initiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans.

Catalytic activityi

UDP-N-acetyl-alpha-D-glucosamine + alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-(alpha-D-Man-(1->6))-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-N-Asn-[protein] = UDP + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-(alpha-D-Man-(1->6))-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-N-Asn-[protein].

Cofactori

Mn2+By similarityNote: The cofactor is mostly bound to the substrate.By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei117SubstrateBy similarity1
Binding sitei144SubstrateBy similarity1
Binding sitei190SubstrateBy similarity1
Binding sitei212SubstrateBy similarity1
Metal bindingi213ManganeseBy similarity1
Active sitei291Proton acceptorSequence analysis1
Binding sitei322SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • in utero embryonic development Source: MGI
  • protein glycosylation Source: UniProtKB-UniPathway
  • UDP-N-acetylglucosamine catabolic process Source: MGI

Keywordsi

Molecular functionGlycosyltransferase, Transferase
LigandManganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-964739 N-glycan trimming and elongation in the cis-Golgi
UniPathwayiUPA00378

Protein family/group databases

CAZyiGT13 Glycosyltransferase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (EC:2.4.1.101)
Alternative name(s):
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I
Short name:
GNT-I
Short name:
GlcNAc-T I
Gene namesi
Name:Mgat1
Synonyms:Gnt1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:96973 Mgat1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 6CytoplasmicSequence analysis6
Transmembranei7 – 29Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST23
Topological domaini30 – 447LumenalSequence analysisAdd BLAST418

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001913851 – 447Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferaseAdd BLAST447

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi115 ↔ 145By similarity
Disulfide bondi239 ↔ 305By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

EPDiP27808
PaxDbiP27808
PeptideAtlasiP27808
PRIDEiP27808

PTM databases

iPTMnetiP27808
PhosphoSitePlusiP27808

Expressioni

Tissue specificityi

Appears to be present in all tissues.

Gene expression databases

BgeeiENSMUSG00000020346
CleanExiMM_MGAT1
ExpressionAtlasiP27808 baseline and differential
GenevisibleiP27808 MM

Interactioni

Subunit structurei

Interacts with MGAT4D.1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000080484

Structurei

3D structure databases

ProteinModelPortaliP27808
SMRiP27808
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 13 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1413 Eukaryota
ENOG410XQD8 LUCA
GeneTreeiENSGT00530000063632
HOGENOMiHOG000247055
HOVERGENiHBG052466
InParanoidiP27808
KOiK00726
OMAiQDPSLWC
OrthoDBiEOG091G0A29
PhylomeDBiP27808
TreeFamiTF320555

Family and domain databases

Gene3Di3.90.550.10, 1 hit
InterProiView protein in InterPro
IPR004139 Glyco_trans_13
IPR029044 Nucleotide-diphossugar_trans
PANTHERiPTHR10468 PTHR10468, 1 hit
PfamiView protein in Pfam
PF03071 GNT-I, 1 hit
SUPFAMiSSF53448 SSF53448, 1 hit

Sequencei

Sequence statusi: Complete.

P27808-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKKQTAGLV LWGAIIFVGW NALLLLFFWT RPAPGRLPSD SALGDDPASL
60 70 80 90 100
TREVIHLAED AEAELERQRG LLQQIKEHYA LWRQRWRVPT VAPPAWPRVP
110 120 130 140 150
VTPSPVQIPI LVIACDRSTV RRCLDKLLHY RPSAERFPII VSQDCGHEET
160 170 180 190 200
AQVIASYGTA VTHIRQPDLS NIAVQPDHRK FQGYYKIARH YRWALGQIFN
210 220 230 240 250
KFKFPAAVVV EDDLEVAPDF FEYFQATYPL LRTDPSLWCV SAWNDNGKEQ
260 270 280 290 300
MVDSSKPELL YRTDFFPGLG WLLLADLWAE LEPKWPKAFW DDWMRRPEQR
310 320 330 340 350
KGRACIRPEI SRTMTFGRKG VSHGQFFDQH LKFIKLNQQF VPFTQLDLSY
360 370 380 390 400
LQQEAYDRDF LAQVYGAPQL QVEKVRTNDQ KELGEVRVQY TSRDSFKAFA
410 420 430 440
KALGVMDDLK SGVPRAGYRG IVTFQFRGRR VHLAPPQTWT GYDPSWN
Length:447
Mass (Da):51,690
Last modified:August 1, 1992 - v1
Checksum:iE7C65EA8C8B34E6A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73491 Genomic DNA Translation: AAA37698.1
L07037 mRNA Translation: AAA40478.1
AK087959 mRNA Translation: BAC40058.1
BC006629 mRNA Translation: AAH06629.1
CCDSiCCDS24602.1
PIRiA42500
RefSeqiNP_001103618.1, NM_001110148.1
NP_001103619.1, NM_001110149.1
NP_001103620.1, NM_001110150.1
NP_034924.3, NM_010794.3
XP_006532460.1, XM_006532397.3
XP_006532461.1, XM_006532398.3
XP_006532462.1, XM_006532399.2
XP_006532463.1, XM_006532400.3
XP_017169803.1, XM_017314314.1
UniGeneiMm.196933

Genome annotation databases

EnsembliENSMUST00000081794; ENSMUSP00000080484; ENSMUSG00000020346
ENSMUST00000101293; ENSMUSP00000098851; ENSMUSG00000020346
ENSMUST00000109194; ENSMUSP00000104817; ENSMUSG00000020346
ENSMUST00000167400; ENSMUSP00000126303; ENSMUSG00000020346
GeneIDi17308
KEGGimmu:17308
UCSCiuc007iqb.2 mouse

Similar proteinsi

Entry informationi

Entry nameiMGAT1_MOUSE
AccessioniPrimary (citable) accession number: P27808
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: March 28, 2018
This is version 154 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health