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P27808

- MGAT1_MOUSE

UniProt

P27808 - MGAT1_MOUSE

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Protein

Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase

Gene
Mgat1, Gnt1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Initiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans.

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 3-(alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 3-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.

Cofactori

Manganese. The cofactor is mostly bound to the substrate By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei117 – 1171Substrate By similarity
Binding sitei144 – 1441Substrate By similarity
Binding sitei190 – 1901Substrate By similarity
Binding sitei212 – 2121Substrate By similarity
Metal bindingi213 – 2131Manganese By similarity
Active sitei291 – 2911Proton acceptor Reviewed prediction
Binding sitei322 – 3221Substrate By similarity

GO - Molecular functioni

  1. acetylglucosaminyltransferase activity Source: MGI
  2. alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. in utero embryonic development Source: MGI
  2. protein glycosylation Source: UniProtKB-UniPathway
  3. UDP-N-acetylglucosamine catabolic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_198544. N-glycan trimming and elongation in the cis-Golgi.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT13. Glycosyltransferase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (EC:2.4.1.101)
Alternative name(s):
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I
Short name:
GNT-I
Short name:
GlcNAc-T I
Gene namesi
Name:Mgat1
Synonyms:Gnt1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:96973. Mgat1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66Cytoplasmic Reviewed prediction
Transmembranei7 – 2923Helical; Signal-anchor for type II membrane protein; Reviewed predictionAdd
BLAST
Topological domaini30 – 447418Lumenal Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. Golgi membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 447447Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferasePRO_0000191385Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi115 ↔ 145 By similarity
Disulfide bondi239 ↔ 305 By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiP27808.
PaxDbiP27808.
PRIDEiP27808.

PTM databases

PhosphoSiteiP27808.

Expressioni

Tissue specificityi

Appears to be present in all tissues.

Gene expression databases

ArrayExpressiP27808.
BgeeiP27808.
CleanExiMM_MGAT1.
GenevestigatoriP27808.

Interactioni

Protein-protein interaction databases

MINTiMINT-1836159.

Structurei

3D structure databases

ProteinModelPortaliP27808.
SMRiP27808. Positions 108-446.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG148227.
HOGENOMiHOG000247055.
HOVERGENiHBG052466.
InParanoidiP27808.
KOiK00726.
OMAiHEETAQV.
OrthoDBiEOG7R56SN.
PhylomeDBiP27808.
TreeFamiTF320555.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR004139. Glyco_trans_13.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR10468. PTHR10468. 1 hit.
PfamiPF03071. GNT-I. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

P27808-1 [UniParc]FASTAAdd to Basket

« Hide

MLKKQTAGLV LWGAIIFVGW NALLLLFFWT RPAPGRLPSD SALGDDPASL    50
TREVIHLAED AEAELERQRG LLQQIKEHYA LWRQRWRVPT VAPPAWPRVP 100
VTPSPVQIPI LVIACDRSTV RRCLDKLLHY RPSAERFPII VSQDCGHEET 150
AQVIASYGTA VTHIRQPDLS NIAVQPDHRK FQGYYKIARH YRWALGQIFN 200
KFKFPAAVVV EDDLEVAPDF FEYFQATYPL LRTDPSLWCV SAWNDNGKEQ 250
MVDSSKPELL YRTDFFPGLG WLLLADLWAE LEPKWPKAFW DDWMRRPEQR 300
KGRACIRPEI SRTMTFGRKG VSHGQFFDQH LKFIKLNQQF VPFTQLDLSY 350
LQQEAYDRDF LAQVYGAPQL QVEKVRTNDQ KELGEVRVQY TSRDSFKAFA 400
KALGVMDDLK SGVPRAGYRG IVTFQFRGRR VHLAPPQTWT GYDPSWN 447
Length:447
Mass (Da):51,690
Last modified:August 1, 1992 - v1
Checksum:iE7C65EA8C8B34E6A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M73491 Genomic DNA. Translation: AAA37698.1.
L07037 mRNA. Translation: AAA40478.1.
AK087959 mRNA. Translation: BAC40058.1.
BC006629 mRNA. Translation: AAH06629.1.
CCDSiCCDS24602.1.
PIRiA42500.
RefSeqiNP_001103618.1. NM_001110148.1.
NP_001103619.1. NM_001110149.1.
NP_001103620.1. NM_001110150.1.
NP_034924.3. NM_010794.3.
XP_006532460.1. XM_006532397.1.
XP_006532461.1. XM_006532398.1.
XP_006532462.1. XM_006532399.1.
XP_006532463.1. XM_006532400.1.
XP_006532464.1. XM_006532401.1.
UniGeneiMm.196933.

Genome annotation databases

EnsembliENSMUST00000081794; ENSMUSP00000080484; ENSMUSG00000020346.
ENSMUST00000101293; ENSMUSP00000098851; ENSMUSG00000020346.
ENSMUST00000109194; ENSMUSP00000104817; ENSMUSG00000020346.
ENSMUST00000167400; ENSMUSP00000126303; ENSMUSG00000020346.
GeneIDi17308.
KEGGimmu:17308.
UCSCiuc007iqb.2. mouse.

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

alpha-1,3-mannosyl-glycoprotein beta 1,2-GlcNAc T I (Mgat1)

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M73491 Genomic DNA. Translation: AAA37698.1 .
L07037 mRNA. Translation: AAA40478.1 .
AK087959 mRNA. Translation: BAC40058.1 .
BC006629 mRNA. Translation: AAH06629.1 .
CCDSi CCDS24602.1.
PIRi A42500.
RefSeqi NP_001103618.1. NM_001110148.1.
NP_001103619.1. NM_001110149.1.
NP_001103620.1. NM_001110150.1.
NP_034924.3. NM_010794.3.
XP_006532460.1. XM_006532397.1.
XP_006532461.1. XM_006532398.1.
XP_006532462.1. XM_006532399.1.
XP_006532463.1. XM_006532400.1.
XP_006532464.1. XM_006532401.1.
UniGenei Mm.196933.

3D structure databases

ProteinModelPortali P27808.
SMRi P27808. Positions 108-446.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-1836159.

Protein family/group databases

CAZyi GT13. Glycosyltransferase Family 13.

PTM databases

PhosphoSitei P27808.

Proteomic databases

MaxQBi P27808.
PaxDbi P27808.
PRIDEi P27808.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000081794 ; ENSMUSP00000080484 ; ENSMUSG00000020346 .
ENSMUST00000101293 ; ENSMUSP00000098851 ; ENSMUSG00000020346 .
ENSMUST00000109194 ; ENSMUSP00000104817 ; ENSMUSG00000020346 .
ENSMUST00000167400 ; ENSMUSP00000126303 ; ENSMUSG00000020346 .
GeneIDi 17308.
KEGGi mmu:17308.
UCSCi uc007iqb.2. mouse.

Organism-specific databases

CTDi 4245.
MGIi MGI:96973. Mgat1.

Phylogenomic databases

eggNOGi NOG148227.
HOGENOMi HOG000247055.
HOVERGENi HBG052466.
InParanoidi P27808.
KOi K00726.
OMAi HEETAQV.
OrthoDBi EOG7R56SN.
PhylomeDBi P27808.
TreeFami TF320555.

Enzyme and pathway databases

UniPathwayi UPA00378 .
Reactomei REACT_198544. N-glycan trimming and elongation in the cis-Golgi.

Miscellaneous databases

ChiTaRSi MGAT1. mouse.
NextBioi 291856.
PROi P27808.
SOURCEi Search...

Gene expression databases

ArrayExpressi P27808.
Bgeei P27808.
CleanExi MM_MGAT1.
Genevestigatori P27808.

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
InterProi IPR004139. Glyco_trans_13.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view ]
PANTHERi PTHR10468. PTHR10468. 1 hit.
Pfami PF03071. GNT-I. 1 hit.
[Graphical view ]
SUPFAMi SSF53448. SSF53448. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of the mouse UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I gene."
    Pownall S., Kozak C.A., Schappert K.T., Sarkar M., Hull E., Schachter H., Math J.D.
    Genomics 12:699-704(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Cloning and expression of the murine gene and chromosomal location of the human gene encoding N-acetylglucosaminyltransferase I."
    Kumar R., Yang J., Eddy R.L., Byers M.G., Shows T.B., Stanley P.
    Glycobiology 2:383-393(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: NOD.
    Tissue: Thymus.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary gland.

Entry informationi

Entry nameiMGAT1_MOUSE
AccessioniPrimary (citable) accession number: P27808
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: September 3, 2014
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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