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P27801

- PEP3_YEAST

UniProt

P27801 - PEP3_YEAST

Protein

Vacuolar membrane protein PEP3

Gene

PEP3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 132 (01 Oct 2014)
      Sequence version 1 (01 Aug 1992)
      Previous versions | rss
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    Functioni

    Required for vacuolar biogenesis and for trafficking of hydrolase precursors to the vacuole. Mediates transport at the vacuolar membrane where it may be responsible for tethering transport vesicles on the target membranes. Acts as component of the HOPS complex that acts during the docking stage of vacuole fusion. HOPS is an effector for the vacuolar Rab GTPase YPT7 and is required for vacuolar SNARE complex assembly. It remains bound to SNARE complexes after vacuole fusion.2 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri826 – 85126RING-type; atypicalAdd
    BLAST

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. protein binding, bridging Source: SGD
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. Golgi to endosome transport Source: SGD
    2. intracellular protein transport Source: InterPro
    3. late endosome to vacuole transport Source: SGD
    4. regulation of SNARE complex assembly Source: SGD
    5. regulation of vacuole fusion, non-autophagic Source: SGD
    6. vacuole fusion, non-autophagic Source: SGD
    7. vesicle docking involved in exocytosis Source: SGD

    Keywords - Biological processi

    Protein transport, Transport

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32284-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Vacuolar membrane protein PEP3
    Alternative name(s):
    Carboxypeptidase Y-deficient protein 3
    Vacuolar morphogenesis protein 8
    Vacuolar protein sorting-associated protein 18
    Vacuolar protein-targeting protein 18
    Gene namesi
    Name:PEP3
    Synonyms:VAM8, VPS18, VPT18
    Ordered Locus Names:YLR148W
    ORF Names:L9634.2
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XII

    Organism-specific databases

    CYGDiYLR148w.
    SGDiS000004138. PEP3.

    Subcellular locationi

    GO - Cellular componenti

    1. CORVET complex Source: SGD
    2. extrinsic component of vacuolar membrane Source: SGD
    3. fungal-type vacuole membrane Source: SGD
    4. HOPS complex Source: SGD
    5. proteasome storage granule Source: SGD

    Keywords - Cellular componenti

    Membrane, Vacuole

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 918918Vacuolar membrane protein PEP3PRO_0000055993Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei907 – 9071Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP27801.
    PaxDbiP27801.
    PRIDEiP27801.

    Expressioni

    Gene expression databases

    GenevestigatoriP27801.

    Interactioni

    Subunit structurei

    Component of the HOPS complex which is composed of PEP5, VPS16, PEP3, VPS33, VPS39 and VPS41. HOPS associates with phosphoinositides and the PX domain of VAM7. Interacts with PEP5, PEP7 and VAM7.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    PEP5P128687EBI-13130,EBI-6450
    VPS33P207957EBI-13130,EBI-20395

    Protein-protein interaction databases

    BioGridi31417. 64 interactions.
    DIPiDIP-5912N.
    IntActiP27801. 15 interactions.
    MINTiMINT-615177.
    STRINGi4932.YLR148W.

    Structurei

    3D structure databases

    ProteinModelPortaliP27801.
    SMRiP27801. Positions 820-856.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati585 – 741157CHCRAdd
    BLAST

    Sequence similaritiesi

    Belongs to the VPS18 family.Curated
    Contains 1 CHCR (clathrin heavy-chain) repeat.PROSITE-ProRule annotation
    Contains 1 RING-type zinc finger.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri826 – 85126RING-type; atypicalAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiNOG246118.
    GeneTreeiENSGT00390000015233.
    HOGENOMiHOG000001125.
    OMAiQIARTRV.
    OrthoDBiEOG7673K5.

    Family and domain databases

    Gene3Di3.30.40.10. 1 hit.
    InterProiIPR000547. Clathrin_H-chain/VPS_repeat.
    IPR007810. Pep3_Vps18.
    IPR019453. VPS39/TGF_beta_rcpt-assoc_2.
    IPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view]
    PfamiPF00637. Clathrin. 1 hit.
    PF05131. Pep3_Vps18. 1 hit.
    PF10367. Vps39_2. 1 hit.
    [Graphical view]
    SMARTiSM00299. CLH. 1 hit.
    SM00184. RING. 1 hit.
    [Graphical view]
    PROSITEiPS50236. CHCR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P27801-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIKTRIEEVQ LQFLTGNTEL THLKVSNDQL IVTTQRTIYR INLQDPAIVN    50
    HFDCPLSKEL ETIMNVHVSP MGSVILIRTN FGRYMLLKDG EFTQLNKIKN 100
    LDLSSLHWIN ETTFLMGIKK TPKLYRVELT GKDITTKLWY ENKKLSGGID 150
    GIAYWEGSLL LTIKDNILYW RDVTNMKFPL VLPDESEQFE RLKHHAIKKF 200
    DSYNGLFAWV TSNGIVFGDL KEKQMEKDPA SNNFGKFLSS SKVLLNFELP 250
    DYQNDKDHLI KDIVLTAFHI LLLRKNTVTM VSQLNNDVVF HETIPRHQLT 300
    GSNTDSNEKF LGLVRDSVKE TFWCFSNINV FEIIIENEPN SVWNLLVRDN 350
    KFDKALSLKG LTVREIESVK LSKAMYLFHT AKDFHSAAQT LGSMKDLSHF 400
    GEIALNFLQI KDYNDLNVIL IKQLDNVPWK STQVVLSSWI IWNFMKQLND 450
    IELKINTTKP ASTDEDNLLN WNLNLKEKSN ELTKFLESHL EKLDNETVYQ 500
    IMSKQNRQNE LLIFASLIND MKFLLSFWID QGNWYESLKI LLTINNHDLV 550
    YKYSLILLLN SPEATVSTWM KIKDLDPNKL IPTILKFFTN WQNNSKLITN 600
    ISEYPENYSL TYLKWCVREV PKMCNPIVYN SILYMMITDP RNDMILENDI 650
    IKFMKSNENK YDLNFQLRLS LKFKKTKTSI FLLTRLNLFE DAIDLALKNN 700
    LIDDCKVIVN DEILIEDYKL RKRLWLKIAK HLLLSMKDID IKQLIRTILN 750
    DSNEILTIKD LLPFFNEYTT IANLKEELIK FLENHNMKMN EISEDIINSK 800
    NLKVEINTEI SKFNEIYRIL EPGKSCDECG KFLQIKKFIV FPCGHCFHWN 850
    CIIRVILNSN DYNLRQKTEN FLKAKSKHNL NDLENIIVEK CGLCSDININ 900
    KIDQPISIDE TELAKWNE 918
    Length:918
    Mass (Da):107,398
    Last modified:August 1, 1992 - v1
    Checksum:i37AE3C34AE0470B8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M65144 Genomic DNA. Translation: AAA34852.1.
    Z73320 Genomic DNA. Translation: CAA97720.1.
    U53879 Genomic DNA. Translation: AAB82382.1.
    BK006945 Genomic DNA. Translation: DAA09459.1.
    PIRiA41943.
    RefSeqiNP_013249.1. NM_001182035.1.

    Genome annotation databases

    EnsemblFungiiYLR148W; YLR148W; YLR148W.
    GeneIDi850840.
    KEGGisce:YLR148W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M65144 Genomic DNA. Translation: AAA34852.1 .
    Z73320 Genomic DNA. Translation: CAA97720.1 .
    U53879 Genomic DNA. Translation: AAB82382.1 .
    BK006945 Genomic DNA. Translation: DAA09459.1 .
    PIRi A41943.
    RefSeqi NP_013249.1. NM_001182035.1.

    3D structure databases

    ProteinModelPortali P27801.
    SMRi P27801. Positions 820-856.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31417. 64 interactions.
    DIPi DIP-5912N.
    IntActi P27801. 15 interactions.
    MINTi MINT-615177.
    STRINGi 4932.YLR148W.

    Proteomic databases

    MaxQBi P27801.
    PaxDbi P27801.
    PRIDEi P27801.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YLR148W ; YLR148W ; YLR148W .
    GeneIDi 850840.
    KEGGi sce:YLR148W.

    Organism-specific databases

    CYGDi YLR148w.
    SGDi S000004138. PEP3.

    Phylogenomic databases

    eggNOGi NOG246118.
    GeneTreei ENSGT00390000015233.
    HOGENOMi HOG000001125.
    OMAi QIARTRV.
    OrthoDBi EOG7673K5.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32284-MONOMER.

    Miscellaneous databases

    NextBioi 967123.
    PROi P27801.

    Gene expression databases

    Genevestigatori P27801.

    Family and domain databases

    Gene3Di 3.30.40.10. 1 hit.
    InterProi IPR000547. Clathrin_H-chain/VPS_repeat.
    IPR007810. Pep3_Vps18.
    IPR019453. VPS39/TGF_beta_rcpt-assoc_2.
    IPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view ]
    Pfami PF00637. Clathrin. 1 hit.
    PF05131. Pep3_Vps18. 1 hit.
    PF10367. Vps39_2. 1 hit.
    [Graphical view ]
    SMARTi SM00299. CLH. 1 hit.
    SM00184. RING. 1 hit.
    [Graphical view ]
    PROSITEi PS50236. CHCR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and characterization of PEP3, a gene required for vacuolar biogenesis in Saccharomyces cerevisiae."
      Preston R., Manolson M.F., Becherer K., Weindnhammer E., Kirkpatrick D., Wright R., Jones E.W.
      Mol. Cell. Biol. 11:5801-5812(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "A putative zinc finger protein, Saccharomyces cerevisiae Vps18p, affects late Golgi functions required for vacuolar protein sorting and efficient alpha-factor prohormone maturation."
      Robinson J.S., Graham T.R., Emr S.D.
      Mol. Cell. Biol. 11:5813-5824(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
      Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
      , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
      Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Pep3p/Pep5p complex: a putative docking factor at multiple steps of vesicular transport to the vacuole of Saccharomyces cerevisiae."
      Srivastava A., Woolford C.A., Jones E.W.
      Genetics 156:105-122(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH PEP5 AND PEP7.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "Purification of active HOPS complex reveals its affinities for phosphoinositides and the SNARE Vam7p."
      Stroupe C., Collins K.M., Fratti R.A., Wickner W.
      EMBO J. 25:1579-1589(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE HOPS COMPLEX, FUNCTION OF THE HOPS COMPLEX, INTERACTION WITH VAM7.
    8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-907, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-907, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPEP3_YEAST
    AccessioniPrimary (citable) accession number: P27801
    Secondary accession number(s): D6VYE3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: August 1, 1992
    Last modified: October 1, 2014
    This is version 132 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2020 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XII
      Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

    External Data

    Dasty 3