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P27801 (PEP3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 129. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Vacuolar membrane protein PEP3
Alternative name(s):
Carboxypeptidase Y-deficient protein 3
Vacuolar morphogenesis protein 8
Vacuolar protein sorting-associated protein 18
Vacuolar protein-targeting protein 18
Gene names
Name:PEP3
Synonyms:VAM8, VPS18, VPT18
Ordered Locus Names:YLR148W
ORF Names:L9634.2
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length918 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for vacuolar biogenesis and for trafficking of hydrolase precursors to the vacuole. Mediates transport at the vacuolar membrane where it may be responsible for tethering transport vesicles on the target membranes. Acts as component of the HOPS complex that acts during the docking stage of vacuole fusion. HOPS is an effector for the vacuolar Rab GTPase YPT7 and is required for vacuolar SNARE complex assembly. It remains bound to SNARE complexes after vacuole fusion. Ref.5 Ref.7

Subunit structure

Component of the HOPS complex which is composed of PEP5, VPS16, PEP3, VPS33, VPS39 and VPS41. HOPS associates with phosphoinositides and the PX domain of VAM7. Interacts with PEP5, PEP7 and VAM7. Ref.5 Ref.7

Subcellular location

Vacuole membrane; Peripheral membrane protein; Cytoplasmic side.

Miscellaneous

Present with 2020 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the VPS18 family.

Contains 1 CHCR (clathrin heavy-chain) repeat.

Contains 1 RING-type zinc finger.

Ontologies

Keywords
   Biological processProtein transport
Transport
   Cellular componentMembrane
Vacuole
   DomainZinc-finger
   LigandMetal-binding
Zinc
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processGolgi to endosome transport

Inferred from genetic interaction PubMed 11422941. Source: SGD

intracellular protein transport

Inferred from electronic annotation. Source: InterPro

late endosome to vacuole transport

Inferred from genetic interaction PubMed 11422941. Source: SGD

regulation of SNARE complex assembly

Inferred from direct assay PubMed 18385512. Source: SGD

regulation of vacuole fusion, non-autophagic

Inferred from direct assay PubMed 18385512. Source: SGD

vacuole fusion, non-autophagic

Inferred from direct assay PubMed 10944212. Source: SGD

vesicle docking involved in exocytosis

Inferred from mutant phenotype Ref.5. Source: SGD

   Cellular_componentCORVET complex

Inferred from direct assay PubMed 17488625. Source: SGD

HOPS complex

Inferred from physical interaction PubMed 10944212. Source: SGD

extrinsic component of vacuolar membrane

Inferred from direct assay Ref.1. Source: SGD

fungal-type vacuole membrane

Inferred from direct assay PubMed 10944212. Source: SGD

proteasome storage granule

Inferred by curator PubMed 9087403. Source: SGD

   Molecular_functionprotein binding, bridging

Inferred from mutant phenotype PubMed 11030345. Source: SGD

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

PEP5P128687EBI-13130,EBI-6450
VPS33P207957EBI-13130,EBI-20395

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 918918Vacuolar membrane protein PEP3
PRO_0000055993

Regions

Repeat585 – 741157CHCR
Zinc finger826 – 85126RING-type; atypical

Amino acid modifications

Modified residue9071Phosphoserine Ref.8 Ref.9

Sequences

Sequence LengthMass (Da)Tools
P27801 [UniParc].

Last modified August 1, 1992. Version 1.
Checksum: 37AE3C34AE0470B8

FASTA918107,398
        10         20         30         40         50         60 
MIKTRIEEVQ LQFLTGNTEL THLKVSNDQL IVTTQRTIYR INLQDPAIVN HFDCPLSKEL 

        70         80         90        100        110        120 
ETIMNVHVSP MGSVILIRTN FGRYMLLKDG EFTQLNKIKN LDLSSLHWIN ETTFLMGIKK 

       130        140        150        160        170        180 
TPKLYRVELT GKDITTKLWY ENKKLSGGID GIAYWEGSLL LTIKDNILYW RDVTNMKFPL 

       190        200        210        220        230        240 
VLPDESEQFE RLKHHAIKKF DSYNGLFAWV TSNGIVFGDL KEKQMEKDPA SNNFGKFLSS 

       250        260        270        280        290        300 
SKVLLNFELP DYQNDKDHLI KDIVLTAFHI LLLRKNTVTM VSQLNNDVVF HETIPRHQLT 

       310        320        330        340        350        360 
GSNTDSNEKF LGLVRDSVKE TFWCFSNINV FEIIIENEPN SVWNLLVRDN KFDKALSLKG 

       370        380        390        400        410        420 
LTVREIESVK LSKAMYLFHT AKDFHSAAQT LGSMKDLSHF GEIALNFLQI KDYNDLNVIL 

       430        440        450        460        470        480 
IKQLDNVPWK STQVVLSSWI IWNFMKQLND IELKINTTKP ASTDEDNLLN WNLNLKEKSN 

       490        500        510        520        530        540 
ELTKFLESHL EKLDNETVYQ IMSKQNRQNE LLIFASLIND MKFLLSFWID QGNWYESLKI 

       550        560        570        580        590        600 
LLTINNHDLV YKYSLILLLN SPEATVSTWM KIKDLDPNKL IPTILKFFTN WQNNSKLITN 

       610        620        630        640        650        660 
ISEYPENYSL TYLKWCVREV PKMCNPIVYN SILYMMITDP RNDMILENDI IKFMKSNENK 

       670        680        690        700        710        720 
YDLNFQLRLS LKFKKTKTSI FLLTRLNLFE DAIDLALKNN LIDDCKVIVN DEILIEDYKL 

       730        740        750        760        770        780 
RKRLWLKIAK HLLLSMKDID IKQLIRTILN DSNEILTIKD LLPFFNEYTT IANLKEELIK 

       790        800        810        820        830        840 
FLENHNMKMN EISEDIINSK NLKVEINTEI SKFNEIYRIL EPGKSCDECG KFLQIKKFIV 

       850        860        870        880        890        900 
FPCGHCFHWN CIIRVILNSN DYNLRQKTEN FLKAKSKHNL NDLENIIVEK CGLCSDININ 

       910 
KIDQPISIDE TELAKWNE 

« Hide

References

« Hide 'large scale' references
[1]"Isolation and characterization of PEP3, a gene required for vacuolar biogenesis in Saccharomyces cerevisiae."
Preston R., Manolson M.F., Becherer K., Weindnhammer E., Kirkpatrick D., Wright R., Jones E.W.
Mol. Cell. Biol. 11:5801-5812(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"A putative zinc finger protein, Saccharomyces cerevisiae Vps18p, affects late Golgi functions required for vacuolar protein sorting and efficient alpha-factor prohormone maturation."
Robinson J.S., Graham T.R., Emr S.D.
Mol. Cell. Biol. 11:5813-5824(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. expand/collapse author list , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Pep3p/Pep5p complex: a putative docking factor at multiple steps of vesicular transport to the vacuole of Saccharomyces cerevisiae."
Srivastava A., Woolford C.A., Jones E.W.
Genetics 156:105-122(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH PEP5 AND PEP7.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Purification of active HOPS complex reveals its affinities for phosphoinositides and the SNARE Vam7p."
Stroupe C., Collins K.M., Fratti R.A., Wickner W.
EMBO J. 25:1579-1589(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE HOPS COMPLEX, FUNCTION OF THE HOPS COMPLEX, INTERACTION WITH VAM7.
[8]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-907, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-907, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M65144 Genomic DNA. Translation: AAA34852.1.
Z73320 Genomic DNA. Translation: CAA97720.1.
U53879 Genomic DNA. Translation: AAB82382.1.
BK006945 Genomic DNA. Translation: DAA09459.1.
PIRA41943.
RefSeqNP_013249.1. NM_001182035.1.

3D structure databases

ProteinModelPortalP27801.
SMRP27801. Positions 821-856.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31417. 64 interactions.
DIPDIP-5912N.
IntActP27801. 15 interactions.
MINTMINT-615177.
STRING4932.YLR148W.

Proteomic databases

PaxDbP27801.
PRIDEP27801.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYLR148W; YLR148W; YLR148W.
GeneID850840.
KEGGsce:YLR148W.

Organism-specific databases

CYGDYLR148w.
SGDS000004138. PEP3.

Phylogenomic databases

eggNOGNOG246118.
GeneTreeENSGT00390000015233.
HOGENOMHOG000001125.
OMAQIARTRV.
OrthoDBEOG7673K5.

Enzyme and pathway databases

BioCycYEAST:G3O-32284-MONOMER.

Gene expression databases

GenevestigatorP27801.

Family and domain databases

Gene3D3.30.40.10. 1 hit.
InterProIPR000547. Clathrin_H-chain/VPS_repeat.
IPR007810. Pep3_Vps18.
IPR019453. VPS39/TGF_beta_rcpt-assoc_2.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamPF00637. Clathrin. 1 hit.
PF05131. Pep3_Vps18. 1 hit.
PF10367. Vps39_2. 1 hit.
[Graphical view]
SMARTSM00299. CLH. 1 hit.
SM00184. RING. 1 hit.
[Graphical view]
PROSITEPS50236. CHCR. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio967123.
PROP27801.

Entry information

Entry namePEP3_YEAST
AccessionPrimary (citable) accession number: P27801
Secondary accession number(s): D6VYE3
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: March 19, 2014
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XII

Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families