P27801 (PEP3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vacuolar membrane protein PEP3

Alternative name(s):
Carboxypeptidase Y-deficient protein 3
Vacuolar morphogenesis protein 8
Vacuolar protein sorting-associated protein 18
Vacuolar protein-targeting protein 18

Gene names

Name:	PEP3
Synonyms:	VAM8, VPS18, VPT18
Ordered Locus Names:	YLR148W
ORF Names:	L9634.2

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	918 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for vacuolar biogenesis and for trafficking of hydrolase precursors to the vacuole. Mediates transport at the vacuolar membrane where it may be responsible for tethering transport vesicles on the target membranes. Acts as component of the HOPS complex that acts during the docking stage of vacuole fusion. HOPS is an effector for the vacuolar Rab GTPase YPT7 and is required for vacuolar SNARE complex assembly. It remains bound to SNARE complexes after vacuole fusion. Ref.5 Ref.7
Subunit structure	Component of the HOPS complex which is composed of PEP5, VPS16, PEP3, VPS33, VPS39 and VPS41. HOPS associates with phosphoinositides and the PX domain of VAM7. Interacts with PEP5, PEP7 and VAM7. Ref.5 Ref.7
Subcellular location	Vacuole membrane; Peripheral membrane protein; Cytoplasmic side.
Miscellaneous	Present with 2020 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the VPS18 family. Contains 1 clathrin repeat. Contains 1 RING-type zinc finger.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Membrane Vacuole
Domain	Zinc-finger
Ligand	Metal-binding Zinc
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Golgi to endosome transport Inferred from genetic interaction PubMed 11422941. Source: SGD intracellular protein transport Inferred from electronic annotation. Source: InterPro late endosome to vacuole transport Inferred from genetic interaction PubMed 11422941. Source: SGD regulation of SNARE complex assembly Inferred from direct assay PubMed 18385512. Source: SGD regulation of vacuole fusion, non-autophagic Inferred from direct assay PubMed 18385512. Source: SGD vacuole fusion, non-autophagic Inferred from mutant phenotype PubMed 10944212. Source: SGD vesicle docking involved in exocytosis Inferred from mutant phenotype Ref.5. Source: SGD
Cellular_component	CORVET complex Inferred from direct assay PubMed 17488625. Source: SGD HOPS complex Inferred from physical interaction PubMed 10944212. Source: SGD extrinsic to vacuolar membrane Inferred from direct assay Ref.1. Source: SGD fungal-type vacuole membrane Inferred from direct assay PubMed 10944212. Source: SGD proteasome storage granule Inferred by curator PubMed 9087403. Source: SGD
Molecular_function	protein binding, bridging Inferred from mutant phenotype PubMed 11030345. Source: SGD zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PEP5	P12868	7	EBI-13130,EBI-6450

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 918	918	Vacuolar membrane protein PEP3		PRO_0000055993
Regions
	Zinc finger	826 – 851	26	RING-type; atypical
Amino acid modifications
	Modified residue	907	1	Phosphoserine Ref.8

Sequences

	Sequence		Length	Mass (Da)
	P27801 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 37AE3C34AE0470B8	FASTA	918	107,398
	10 20 30 40 50 60 MIKTRIEEVQ LQFLTGNTEL THLKVSNDQL IVTTQRTIYR INLQDPAIVN HFDCPLSKEL 70 80 90 100 110 120 ETIMNVHVSP MGSVILIRTN FGRYMLLKDG EFTQLNKIKN LDLSSLHWIN ETTFLMGIKK 130 140 150 160 170 180 TPKLYRVELT GKDITTKLWY ENKKLSGGID GIAYWEGSLL LTIKDNILYW RDVTNMKFPL 190 200 210 220 230 240 VLPDESEQFE RLKHHAIKKF DSYNGLFAWV TSNGIVFGDL KEKQMEKDPA SNNFGKFLSS 250 260 270 280 290 300 SKVLLNFELP DYQNDKDHLI KDIVLTAFHI LLLRKNTVTM VSQLNNDVVF HETIPRHQLT 310 320 330 340 350 360 GSNTDSNEKF LGLVRDSVKE TFWCFSNINV FEIIIENEPN SVWNLLVRDN KFDKALSLKG 370 380 390 400 410 420 LTVREIESVK LSKAMYLFHT AKDFHSAAQT LGSMKDLSHF GEIALNFLQI KDYNDLNVIL 430 440 450 460 470 480 IKQLDNVPWK STQVVLSSWI IWNFMKQLND IELKINTTKP ASTDEDNLLN WNLNLKEKSN 490 500 510 520 530 540 ELTKFLESHL EKLDNETVYQ IMSKQNRQNE LLIFASLIND MKFLLSFWID QGNWYESLKI 550 560 570 580 590 600 LLTINNHDLV YKYSLILLLN SPEATVSTWM KIKDLDPNKL IPTILKFFTN WQNNSKLITN 610 620 630 640 650 660 ISEYPENYSL TYLKWCVREV PKMCNPIVYN SILYMMITDP RNDMILENDI IKFMKSNENK 670 680 690 700 710 720 YDLNFQLRLS LKFKKTKTSI FLLTRLNLFE DAIDLALKNN LIDDCKVIVN DEILIEDYKL 730 740 750 760 770 780 RKRLWLKIAK HLLLSMKDID IKQLIRTILN DSNEILTIKD LLPFFNEYTT IANLKEELIK 790 800 810 820 830 840 FLENHNMKMN EISEDIINSK NLKVEINTEI SKFNEIYRIL EPGKSCDECG KFLQIKKFIV 850 860 870 880 890 900 FPCGHCFHWN CIIRVILNSN DYNLRQKTEN FLKAKSKHNL NDLENIIVEK CGLCSDININ 910 KIDQPISIDE TELAKWNE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of PEP3, a gene required for vacuolar biogenesis in Saccharomyces cerevisiae." Preston R., Manolson M.F., Becherer K., Weindnhammer E., Kirkpatrick D., Wright R., Jones E.W. Mol. Cell. Biol. 11:5801-5812(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A putative zinc finger protein, Saccharomyces cerevisiae Vps18p, affects late Golgi functions required for vacuolar protein sorting and efficient alpha-factor prohormone maturation." Robinson J.S., Graham T.R., Emr S.D. Mol. Cell. Biol. 11:5813-5824(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Pep3p/Pep5p complex: a putative docking factor at multiple steps of vesicular transport to the vacuole of Saccharomyces cerevisiae." Srivastava A., Woolford C.A., Jones E.W. Genetics 156:105-122(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH PEP5 AND PEP7.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Purification of active HOPS complex reveals its affinities for phosphoinositides and the SNARE Vam7p." Stroupe C., Collins K.M., Fratti R.A., Wickner W. EMBO J. 25:1579-1589(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE HOPS COMPLEX, FUNCTION OF THE HOPS COMPLEX, INTERACTION WITH VAM7.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-907, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M65144 Genomic DNA. Translation: AAA34852.1. Z73320 Genomic DNA. Translation: CAA97720.1. U53879 Genomic DNA. Translation: AAB82382.1. BK006945 Genomic DNA. Translation: DAA09459.1.
PIR	A41943.
RefSeq	NP_013249.1. NM_001182035.1.
3D structure databases
ProteinModelPortal	P27801.
SMR	P27801. Positions 820-856.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5912N.
IntAct	P27801. 14 interactions.
MINT	MINT-615177.
STRING	4932.YLR148W.
Proteomic databases
PaxDb	P27801.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YLR148W; YLR148W; YLR148W.
GeneID	850840.
KEGG	sce:YLR148W.
Organism-specific databases
CYGD	YLR148w.
SGD	S000004138. PEP3.
Phylogenomic databases
eggNOG	NOG246118.
GeneTree	ENSGT00390000015233.
HOGENOM	HOG000001125.
OMA	QIARTRV.
OrthoDB	EOG4SJ8NX.
Gene expression databases
Genevestigator	P27801.
GermOnline	YLR148W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.30.40.10. 1 hit.
InterPro	IPR000547. Clathrin_H-chain/VPS_repeat. IPR007810. Pep3_Vps18. IPR019453. VPS39/TGF_beta_rcpt-assoc_2. IPR001841. Znf_RING. IPR013083. Znf_RING/FYVE/PHD. [Graphical view]
Pfam	PF00637. Clathrin. 1 hit. PF05131. Pep3_Vps18. 1 hit. PF10367. Vps39_2. 1 hit. [Graphical view]
SMART	SM00299. CLH. 1 hit. SM00184. RING. 1 hit. [Graphical view]
PROSITE	PS00518. ZF_RING_1. False negative. PS50089. ZF_RING_2. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	967123.

Entry information

Entry name

PEP3_YEAST

Accession

Primary (citable) accession number: P27801
Secondary accession number(s): D6VYE3

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	May 1, 2013
This is version 122 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program