P27800 (ALDX_SPOSA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aldehyde reductase 1
Short name=ALR 1
EC=1.1.1.2

Alternative name(s):
Alcohol dehydrogenase [NADP+]
Aldehyde reductase I

Gene names

Name:

ARI

Organism

Sporobolomyces salmonicolor

Taxonomic identifier

5005 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Pucciniomycotina › Microbotryomycetes › Sporidiobolales › Sporidiobolus

Protein attributes

Sequence length	323 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the asymmetric reduction of aliphatic and aromatic aldehydes and ketones to an R-enantiomer. Reduces ethyl 4-chloro-3-oxobutanoate to ethyl (R)-4-chloro-3-hydroxybutanoate. Ref.1
Catalytic activity	An alcohol + NADP+ = an aldehyde + NADPH.
Enzyme regulation	Inhibited by quercetin, dicoumarol and some SH-reagents. Ref.2
Subunit structure	Monomer.
Sequence similarities	Belongs to the aldo/keto reductase family.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	cell Inferred from direct assay Ref.1. Source: UniProtKB
Molecular function	alcohol dehydrogenase (NADP+) activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.2
	Chain	2 – 323	322	Aldehyde reductase 1		PRO_0000124621
Regions
	Nucleotide binding	210 – 267	58	NADP By similarity
Sites
	Active site	49	1	Proton donor By similarity
	Binding site	107	1	Substrate By similarity
	Site	74	1	Lowers pKa of active site Tyr By similarity

Sequences

	Sequence		Length	Mass (Da)
	P27800 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: D49B33ACB41D4D60	FASTA	323	35,339
	10 20 30 40 50 60 MVGTTTLNTG ASLELVGYGT WQAAPGEVGQ GVKVAIETGY RHLDLAKVYS NQPEVGAAIK 70 80 90 100 110 120 EAGVKREDLF ITSKLWNNSH RPEQVEPALD DTLKELGLEY LDLYLIHWPV AFPPEGDITQ 130 140 150 160 170 180 NLFPKANDKE VKLDLEVSLV DTWKAMVKLL DTGKVKAIGV SNFDAKMVDA IIEATGVTPS 190 200 210 220 230 240 VNQIERHPLL LQPELIAHHK AKNIHITAYS PLGNNTVGAP LLVQHPEIKR IAEKNGCTPA 250 260 270 280 290 300 QVLIAWAIVG GHSVIPKSVT PSRIGENFKQ VSLSQEDVDA VSKLGEGSGR RRYNIPCTYS 310 320 PKWDINVFGE EDEKSCKNAV KIK

References

[1]	"Cloning of the aldehyde reductase gene from a red yeast, Sporobolomyces salmonicolor, and characterization of the gene and its product." Kita K., Matsuzaki K., Hashimoto T., Yanase H., Kato N., Chung M.C.-M., Kataoka M., Shimizu S. Appl. Environ. Microbiol. 62:2303-2310(1996) [PubMed: 8779568] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 34-125; 133-154; 157-200 AND 203-314, FUNCTION. Strain: AKU 4429.
[2]	"Characterization of aldehyde reductase of Sporobolomyces salmonicolor." Kataoka M., Sakai H., Morikawa T., Katoh M., Miyoshi T., Shimizu S., Yamada H. Biochim. Biophys. Acta 1122:57-62(1992) [PubMed: 1633196] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-52, ENZYME REGULATION. Strain: AKU 4429.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U26463 Genomic DNA. Translation: AAB17362.1.
PIR	S78113.
3D structure databases
ProteinModelPortal	P27800.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001395. Aldo/ket_red. IPR018170. Aldo/ket_reductase_CS. IPR020471. Aldo/keto_reductase_subgr. IPR023210. NADP_OxRdtase_dom. [Graphical view]
Gene3D	G3DSA:3.20.20.100. Aldo/ket_red. 1 hit.
PANTHER	PTHR11732. Aldo/ket_red. 1 hit.
Pfam	PF00248. Aldo_ket_red. 1 hit. [Graphical view]
PIRSF	PIRSF000097. AKR. 1 hit.
PRINTS	PR00069. ALDKETRDTASE.
SUPFAM	SSF51430. Aldo/ket_red. 1 hit.
PROSITE	PS00798. ALDOKETO_REDUCTASE_1. 1 hit. PS00062. ALDOKETO_REDUCTASE_2. 1 hit. PS00063. ALDOKETO_REDUCTASE_3. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALDX_SPOSA

Accession

Primary (citable) accession number: P27800
Secondary accession number(s): Q12707

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	January 23, 2007
Last modified:	September 21, 2011
This is version 74 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program