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P27790

- CENPB_MOUSE

UniProt

P27790 - CENPB_MOUSE

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Protein

Major centromere autoantigen B

Gene

Cenpb

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Interacts with centromeric heterochromatin in chromosomes and binds to a specific subset of alphoid satellite DNA, called the CENP-B box. May organize arrays of centromere satellite DNA into a higher-order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi28 – 4821H-T-H motifBy similarityAdd
BLAST
DNA bindingi97 – 12933H-T-H motifBy similarityAdd
BLAST

GO - Molecular functioni

  1. chromatin binding Source: InterPro
  2. DNA binding Source: UniProtKB-KW

GO - Biological processi

  1. regulation of transcription, DNA-templated Source: InterPro
Complete GO annotation...

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Major centromere autoantigen B
Alternative name(s):
Centromere protein B
Short name:
CENP-B
Gene namesi
Name:Cenpb
Synonyms:Cenp-b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:88376. Cenpb.

Subcellular locationi

GO - Cellular componenti

  1. chromosome, centromeric region Source: MGI
  2. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 599598Major centromere autoantigen BPRO_0000126126Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N,N,N-trimethylglycineBy similarity
Modified residuei165 – 1651PhosphoserineBy similarity
Modified residuei398 – 3981PhosphothreonineBy similarity

Post-translational modificationi

Poly-ADP-ribosylated by PARP1.
N-terminally methylated by METTL11A/NTM1. Alpha-N-methylation is stimulated in response to extracellular stimuli, including increased cell density and heat shock, and seems to facilitate binding to CENP-B boxes. Chromatin-bound CENP-B is primarily trimethylated (By similarity).By similarity

Keywords - PTMi

ADP-ribosylation, Methylation, Phosphoprotein

Proteomic databases

MaxQBiP27790.
PRIDEiP27790.

PTM databases

PhosphoSiteiP27790.

Expressioni

Gene expression databases

BgeeiP27790.
CleanExiMM_CENPB.
ExpressionAtlasiP27790. baseline and differential.
GenevestigatoriP27790.

Interactioni

Subunit structurei

Antiparallel homodimer. Interacts with CENPT (By similarity).By similarity

Protein-protein interaction databases

BioGridi198675. 1 interaction.
IntActiP27790. 1 interaction.
MINTiMINT-237475.

Structurei

3D structure databases

ProteinModelPortaliP27790.
SMRiP27790. Positions 1-129, 540-585.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 5251HTH psq-typePROSITE-ProRule annotationAdd
BLAST
Domaini65 – 13672HTH CENPB-typePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni536 – 59964HomodimerizationBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi404 – 46562Glu-rich (acidic)Add
BLAST
Compositional biasi508 – 53831Asp/Glu-rich (acidic)Add
BLAST

Sequence similaritiesi

Contains 1 HTH CENPB-type DNA-binding domain.PROSITE-ProRule annotation
Contains 1 HTH psq-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG241149.
GeneTreeiENSGT00760000119149.
HOGENOMiHOG000111537.
HOVERGENiHBG050890.
InParanoidiP27790.
KOiK11496.
OMAiKRRQLTF.
OrthoDBiEOG7HTHGS.
TreeFamiTF101131.

Family and domain databases

Gene3Di1.10.10.60. 2 hits.
InterProiIPR015115. Centromere_CenpB_dimerisation.
IPR004875. DDE_SF_endonuclease_CENPB-like.
IPR009057. Homeodomain-like.
IPR006600. HTH_CenpB_DNA-bd_dom.
IPR007889. HTH_Psq.
[Graphical view]
PfamiPF09026. CENP-B_dimeris. 1 hit.
PF04218. CENP-B_N. 1 hit.
PF03184. DDE_1. 1 hit.
PF03221. HTH_Tnp_Tc5. 1 hit.
[Graphical view]
SMARTiSM00674. CENPB. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 2 hits.
PROSITEiPS51253. HTH_CENPB. 1 hit.
PS50960. HTH_PSQ. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27790-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGPKRRQLTF REKSRIIQEV EENPDLRKGE IARRFNIPPS TLSTILKNKR
60 70 80 90 100
AILASERKYG VASTCRKTNK LSPYDKLEGL LIAWFQQIRA AGLPVKGIIL
110 120 130 140 150
KEKALRIAEE LGMDDFTASN GWLDRFRRRH GVVACSGVTR SRARSSAPRA
160 170 180 190 200
PAAPAGPATV PSEGSGGSTP GWHTREEQPP SVAEGYASQD VFSATETSLW
210 220 230 240 250
YDFLSDQASG LWGGDGPARQ ATQRLSVLLC ANADGSEKLP PLVAGKSAKP
260 270 280 290 300
RAGQGGLPCD YTANSKGGVT TQALAKYLKA LDTRMAAESR RVLLLAGRLA
310 320 330 340 350
AQSLDTSGLR HVQLAFFPPG TVHPLERGVV QQVKGHYRQA MLLKAMAALE
360 370 380 390 400
GQDPSGLQLG LVEALHFVAA AWQAVEPSDI ATCFREAGFG GGLNATITTS
410 420 430 440 450
FKSEGEEEEE EEEEEEEEEE EEGEGEEEEE EEEEGEEEGG EGEEEGEEEV
460 470 480 490 500
EEEGEVDDSD EEEEESSSEG LEAEDWAQGV VEASGGFGGY SVQEEAQFPT
510 520 530 540 550
LHFLEGGEDS DSDSDEEEDD EEEDEEDEDE EDDEDGDEVP VPSFGEAMAY
560 570 580 590
FAMVKRYLTS FPIDDRVQSH ILHLEHDLVH VTRKNHARQA GVRGLGHQS
Length:599
Mass (Da):65,381
Last modified:July 27, 2011 - v2
Checksum:iEBDB7C76BA87DC73
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti145 – 1451S → T in CAA38878. (PubMed:1893793)Curated
Sequence conflicti150 – 1523APA → PQP in CAA38878. (PubMed:1893793)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55038 Genomic DNA. Translation: CAA38878.1.
AL831736 Genomic DNA. No translation available.
BC053333 mRNA. Translation: AAH53333.1.
BC071269 mRNA. Translation: AAH71269.1.
BC075733 mRNA. Translation: AAH75733.1.
CCDSiCCDS16757.1.
RefSeqiNP_031708.2. NM_007682.2.
UniGeneiMm.440169.

Genome annotation databases

EnsembliENSMUST00000089510; ENSMUSP00000086938; ENSMUSG00000068267.
GeneIDi12616.
KEGGimmu:12616.
UCSCiuc008mkx.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55038 Genomic DNA. Translation: CAA38878.1 .
AL831736 Genomic DNA. No translation available.
BC053333 mRNA. Translation: AAH53333.1 .
BC071269 mRNA. Translation: AAH71269.1 .
BC075733 mRNA. Translation: AAH75733.1 .
CCDSi CCDS16757.1.
RefSeqi NP_031708.2. NM_007682.2.
UniGenei Mm.440169.

3D structure databases

ProteinModelPortali P27790.
SMRi P27790. Positions 1-129, 540-585.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 198675. 1 interaction.
IntActi P27790. 1 interaction.
MINTi MINT-237475.

PTM databases

PhosphoSitei P27790.

Proteomic databases

MaxQBi P27790.
PRIDEi P27790.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000089510 ; ENSMUSP00000086938 ; ENSMUSG00000068267 .
GeneIDi 12616.
KEGGi mmu:12616.
UCSCi uc008mkx.1. mouse.

Organism-specific databases

CTDi 1059.
MGIi MGI:88376. Cenpb.

Phylogenomic databases

eggNOGi NOG241149.
GeneTreei ENSGT00760000119149.
HOGENOMi HOG000111537.
HOVERGENi HBG050890.
InParanoidi P27790.
KOi K11496.
OMAi KRRQLTF.
OrthoDBi EOG7HTHGS.
TreeFami TF101131.

Miscellaneous databases

NextBioi 281782.
PROi P27790.
SOURCEi Search...

Gene expression databases

Bgeei P27790.
CleanExi MM_CENPB.
ExpressionAtlasi P27790. baseline and differential.
Genevestigatori P27790.

Family and domain databases

Gene3Di 1.10.10.60. 2 hits.
InterProi IPR015115. Centromere_CenpB_dimerisation.
IPR004875. DDE_SF_endonuclease_CENPB-like.
IPR009057. Homeodomain-like.
IPR006600. HTH_CenpB_DNA-bd_dom.
IPR007889. HTH_Psq.
[Graphical view ]
Pfami PF09026. CENP-B_dimeris. 1 hit.
PF04218. CENP-B_N. 1 hit.
PF03184. DDE_1. 1 hit.
PF03221. HTH_Tnp_Tc5. 1 hit.
[Graphical view ]
SMARTi SM00674. CENPB. 1 hit.
[Graphical view ]
SUPFAMi SSF46689. SSF46689. 2 hits.
PROSITEi PS51253. HTH_CENPB. 1 hit.
PS50960. HTH_PSQ. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "CENP-B is a highly conserved mammalian centromere protein with homology to the helix-loop-helix family of proteins."
    Sullivan K.F., Glass C.A.
    Chromosoma 100:360-370(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: C57BL/6.
    Tissue: Liver.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Colon, Embryo, Jaw and Limb.
  4. "Centromere proteins Cenpa, Cenpb, and Bub3 interact with poly(ADP-ribose) polymerase-1 protein and are poly(ADP-ribosyl)ated."
    Saxena A., Saffery R., Wong L.H., Kalitsis P., Choo K.H.
    J. Biol. Chem. 277:26921-26926(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: POLY-ADP-RIBOSYLATION BY PARP1.

Entry informationi

Entry nameiCENPB_MOUSE
AccessioniPrimary (citable) accession number: P27790
Secondary accession number(s): Q7TSG8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: July 27, 2011
Last modified: October 29, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3