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Protein

Major centromere autoantigen B

Gene

Cenpb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Interacts with centromeric heterochromatin in chromosomes and binds to a specific 17 bp subset of alphoid satellite DNA, called the CENP-B box. May organize arrays of centromere satellite DNA into a higher-order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi28 – 48H-T-H motifBy similarityAdd BLAST21
DNA bindingi97 – 129H-T-H motifBy similarityAdd BLAST33

GO - Molecular functioni

Complete GO annotation...

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Major centromere autoantigen B
Alternative name(s):
Centromere protein B
Short name:
CENP-B
Gene namesi
Name:Cenpb
Synonyms:Cenp-b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:88376. Cenpb.

Subcellular locationi

  • Nucleus By similarity
  • Chromosomecentromere By similarity

GO - Cellular componenti

  • chromosome Source: MGI
  • chromosome, centromeric region Source: MGI
  • condensed nuclear chromosome, centromeric region Source: MGI
  • nuclear pericentric heterochromatin Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001261262 – 599Major centromere autoantigen BAdd BLAST598

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N,N,N-trimethylglycineBy similarity1
Modified residuei165PhosphoserineBy similarity1
Modified residuei396PhosphothreonineBy similarity1
Modified residuei398PhosphothreonineBy similarity1

Post-translational modificationi

Poly-ADP-ribosylated by PARP1.1 Publication
N-terminally methylated by METTL11A/NTM1. Alpha-N-methylation is stimulated in response to extracellular stimuli, including increased cell density and heat shock, and seems to facilitate binding to CENP-B boxes. Chromatin-bound CENP-B is primarily trimethylated.By similarity

Keywords - PTMi

ADP-ribosylation, Methylation, Phosphoprotein

Proteomic databases

MaxQBiP27790.
PaxDbiP27790.
PeptideAtlasiP27790.
PRIDEiP27790.

PTM databases

iPTMnetiP27790.
PhosphoSitePlusiP27790.

Expressioni

Gene expression databases

BgeeiENSMUSG00000068267.
CleanExiMM_CENPB.
GenevisibleiP27790. MM.

Interactioni

Subunit structurei

Antiparallel homodimer. Interacts with CENPT. Identified in a centromere complex containing histones H2A, H2B and H4, and at least CENPA, CENPB, CENPC, CENPT, CENPN, HJURP, SUPT16H, SSRP1 and RSF1.By similarity

Protein-protein interaction databases

BioGridi198675. 1 interactor.
IntActiP27790. 1 interactor.
MINTiMINT-237475.
STRINGi10090.ENSMUSP00000086938.

Structurei

3D structure databases

ProteinModelPortaliP27790.
SMRiP27790.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 52HTH psq-typePROSITE-ProRule annotationAdd BLAST51
Domaini65 – 136HTH CENPB-typePROSITE-ProRule annotationAdd BLAST72

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni536 – 599HomodimerizationBy similarityAdd BLAST64

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi404 – 465Glu-rich (acidic)Add BLAST62
Compositional biasi508 – 538Asp/Glu-rich (acidic)Add BLAST31

Sequence similaritiesi

Contains 1 HTH CENPB-type DNA-binding domain.PROSITE-ProRule annotation
Contains 1 HTH psq-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3105. Eukaryota.
ENOG4110CDI. LUCA.
GeneTreeiENSGT00760000119149.
HOGENOMiHOG000111537.
HOVERGENiHBG050890.
InParanoidiP27790.
KOiK11496.
OMAiKRRQLTF.
OrthoDBiEOG091G0G48.
TreeFamiTF101131.

Family and domain databases

Gene3Di1.10.10.60. 2 hits.
InterProiIPR033062. CENP-B.
IPR015115. Centromere_CenpB_dimerisation.
IPR004875. DDE_SF_endonuclease_dom.
IPR009057. Homeodomain-like.
IPR006600. HTH_CenpB_DNA-bd_dom.
IPR007889. HTH_Psq.
[Graphical view]
PANTHERiPTHR19303:SF194. PTHR19303:SF194. 1 hit.
PfamiPF09026. CENP-B_dimeris. 1 hit.
PF04218. CENP-B_N. 1 hit.
PF03184. DDE_1. 1 hit.
PF03221. HTH_Tnp_Tc5. 1 hit.
[Graphical view]
SMARTiSM00674. CENPB. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 2 hits.
PROSITEiPS51253. HTH_CENPB. 1 hit.
PS50960. HTH_PSQ. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27790-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGPKRRQLTF REKSRIIQEV EENPDLRKGE IARRFNIPPS TLSTILKNKR
60 70 80 90 100
AILASERKYG VASTCRKTNK LSPYDKLEGL LIAWFQQIRA AGLPVKGIIL
110 120 130 140 150
KEKALRIAEE LGMDDFTASN GWLDRFRRRH GVVACSGVTR SRARSSAPRA
160 170 180 190 200
PAAPAGPATV PSEGSGGSTP GWHTREEQPP SVAEGYASQD VFSATETSLW
210 220 230 240 250
YDFLSDQASG LWGGDGPARQ ATQRLSVLLC ANADGSEKLP PLVAGKSAKP
260 270 280 290 300
RAGQGGLPCD YTANSKGGVT TQALAKYLKA LDTRMAAESR RVLLLAGRLA
310 320 330 340 350
AQSLDTSGLR HVQLAFFPPG TVHPLERGVV QQVKGHYRQA MLLKAMAALE
360 370 380 390 400
GQDPSGLQLG LVEALHFVAA AWQAVEPSDI ATCFREAGFG GGLNATITTS
410 420 430 440 450
FKSEGEEEEE EEEEEEEEEE EEGEGEEEEE EEEEGEEEGG EGEEEGEEEV
460 470 480 490 500
EEEGEVDDSD EEEEESSSEG LEAEDWAQGV VEASGGFGGY SVQEEAQFPT
510 520 530 540 550
LHFLEGGEDS DSDSDEEEDD EEEDEEDEDE EDDEDGDEVP VPSFGEAMAY
560 570 580 590
FAMVKRYLTS FPIDDRVQSH ILHLEHDLVH VTRKNHARQA GVRGLGHQS
Length:599
Mass (Da):65,381
Last modified:July 27, 2011 - v2
Checksum:iEBDB7C76BA87DC73
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti145S → T in CAA38878 (PubMed:1893793).Curated1
Sequence conflicti150 – 152APA → PQP in CAA38878 (PubMed:1893793).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55038 Genomic DNA. Translation: CAA38878.1.
AL831736 Genomic DNA. No translation available.
BC053333 mRNA. Translation: AAH53333.1.
BC071269 mRNA. Translation: AAH71269.1.
BC075733 mRNA. Translation: AAH75733.1.
CCDSiCCDS16757.1.
RefSeqiNP_031708.2. NM_007682.3.
UniGeneiMm.440169.

Genome annotation databases

EnsembliENSMUST00000089510; ENSMUSP00000086938; ENSMUSG00000068267.
GeneIDi12616.
KEGGimmu:12616.
UCSCiuc008mkx.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55038 Genomic DNA. Translation: CAA38878.1.
AL831736 Genomic DNA. No translation available.
BC053333 mRNA. Translation: AAH53333.1.
BC071269 mRNA. Translation: AAH71269.1.
BC075733 mRNA. Translation: AAH75733.1.
CCDSiCCDS16757.1.
RefSeqiNP_031708.2. NM_007682.3.
UniGeneiMm.440169.

3D structure databases

ProteinModelPortaliP27790.
SMRiP27790.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198675. 1 interactor.
IntActiP27790. 1 interactor.
MINTiMINT-237475.
STRINGi10090.ENSMUSP00000086938.

PTM databases

iPTMnetiP27790.
PhosphoSitePlusiP27790.

Proteomic databases

MaxQBiP27790.
PaxDbiP27790.
PeptideAtlasiP27790.
PRIDEiP27790.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000089510; ENSMUSP00000086938; ENSMUSG00000068267.
GeneIDi12616.
KEGGimmu:12616.
UCSCiuc008mkx.1. mouse.

Organism-specific databases

CTDi1059.
MGIiMGI:88376. Cenpb.

Phylogenomic databases

eggNOGiKOG3105. Eukaryota.
ENOG4110CDI. LUCA.
GeneTreeiENSGT00760000119149.
HOGENOMiHOG000111537.
HOVERGENiHBG050890.
InParanoidiP27790.
KOiK11496.
OMAiKRRQLTF.
OrthoDBiEOG091G0G48.
TreeFamiTF101131.

Miscellaneous databases

PROiP27790.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000068267.
CleanExiMM_CENPB.
GenevisibleiP27790. MM.

Family and domain databases

Gene3Di1.10.10.60. 2 hits.
InterProiIPR033062. CENP-B.
IPR015115. Centromere_CenpB_dimerisation.
IPR004875. DDE_SF_endonuclease_dom.
IPR009057. Homeodomain-like.
IPR006600. HTH_CenpB_DNA-bd_dom.
IPR007889. HTH_Psq.
[Graphical view]
PANTHERiPTHR19303:SF194. PTHR19303:SF194. 1 hit.
PfamiPF09026. CENP-B_dimeris. 1 hit.
PF04218. CENP-B_N. 1 hit.
PF03184. DDE_1. 1 hit.
PF03221. HTH_Tnp_Tc5. 1 hit.
[Graphical view]
SMARTiSM00674. CENPB. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 2 hits.
PROSITEiPS51253. HTH_CENPB. 1 hit.
PS50960. HTH_PSQ. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCENPB_MOUSE
AccessioniPrimary (citable) accession number: P27790
Secondary accession number(s): Q7TSG8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.