Reviewed, UniProtKB/Swiss-Prot P27786 (CP17A_MOUSE)

Names and origin

Protein names

Recommended name:
    Steroid 17-alpha-hydroxylase/17,20 lyase
    EC=1.14.99.9
Alternative name(s):
    Cytochrome P450 17A1
    CYPXVII
    P450-C17
      Short name=P450c17

Gene names

Name:	Cyp17a1
Synonyms:	Cyp17

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	507 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty.
Catalytic activity	A steroid + AH2 + O2 = a 17-alpha-hydroxysteroid + A + H2O.
Cofactor	Heme group By similarity.
Enzyme regulation	Regulated predominantly by intracellular cAMP levels.
Pathway	Lipid metabolism; steroid biosynthesis.
Subcellular location	Membrane Potential. Membrane; Single-pass membrane protein.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Steroidogenesis
Cellular component	Membrane
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	glucocorticoid biosynthetic process Inferred from direct assay. Source: MGI oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	axon Inferred from direct assay. Source: MGI cell soma Inferred from direct assay. Source: MGI integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrion Inferred from direct assay. Source: MGI
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro steroid 17-alpha-monooxygenase activity Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 507	507	Steroid 17-alpha-hydroxylase/17,20 lyase		PRO_0000051935
Sites
	Metal binding	441	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P27786-1 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: C0916193356EFEBD	FASTA	507	57,638
	10 20 30 40 50 60 MWELVGLLLL ILAYFFWPKS KTPNAKFPRS LPFLPLVGSL PFLPRRGHMH ANFFKLQEKY 70 80 90 100 110 120 GPIYSLRLGT TTAVIVGHYQ LAREVLVKKG KEFSGRPQMV TLGLLSDQGK GVAFADSSSS 130 140 150 160 170 180 WQLHRKLVFS TFSLFRDDQK LEKMICQEAN SLCDLILTYD GESRDLSTLI FKSVINIICT 190 200 210 220 230 240 ICFNISFENK DPILTTIQTF TEGIVDVLGH SDLVDIFPWL KIFPNKNLEM IKEHTKIREK 250 260 270 280 290 300 TLVEMFEKCK EKFNSESLSS LTDILIQAKM NAENNNTGEG QDPSVFSDKH ILVTVGDIFG 310 320 330 340 350 360 AGIETTSSVL NWILAFLVHN PEVKRKIQKE IDQYVGFSRT PSFNDRTHLL MLEATIREVL 370 380 390 400 410 420 RIRPVAPLLI PHKANIDSSI GEFAIPKDTH VIINLWALHH DKNEWDQPDR FMPERFLDPT 430 440 450 460 470 480 GSHLITPTPS YLPFGAGPRS CIGEALARQE LFIFMALLLQ RFDFDVSDDK QLPCLVGDPK 490 500 VVFLIDPFKV KITVRQAWKD AQVEVST

References

[1]	"Isolation, characterization, and chromosomal mapping of mouse P450 17 alpha-hydroxylase/C17-20 lyase." Youngblood G.L., Sartorius C., Taylor B.A., Payne A.H. Genomics 10:270-275(1991) [PubMed: 1840559] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M64863 mRNA. Translation: AAA39877.1.
IPI	IPI00119940.
PIR	A39072.
RefSeq	NP_031835.3.
UniGene	Mm.1262
3D structure databases
HSSP	HSSP built from PDB template 1DT6 based on UniProtKB P00179.
ModBase	Search...
Protein-protein interaction databases
STRING	P27786.
PTM databases
PhosphoSite	P27786.
Proteomic databases
PRIDE	P27786.
Genome annotation databases
Ensembl	ENSMUST00000026012; ENSMUSP00000026012; ENSMUSG00000003555; Mus musculus. [Genome view]
GeneID	13074.
KEGG	mmu:13074.
Organism-specific databases
CTD	13074.
MGI	MGI:88586. Cyp17a1.
Phylogenomic databases
HOGENOM	P27786.
HOVERGEN	P27786.
OMA	NIICTIC.
Enzyme and pathway databases
BRENDA	1.14.99.9. 244.
Gene expression databases
ArrayExpress	P27786.
Bgee	P27786.
Genevestigator	P27786.
GermOnline	ENSMUSG00000003555. Mus musculus.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	283012.
SOURCE	Search...

Entry information

Entry name

CP17A_MOUSE

Accession

Primary (citable) accession number: P27786

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	November 3, 2009
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)