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P27786 (CP17A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Steroid 17-alpha-hydroxylase/17,20 lyase

EC=1.14.99.9
EC=4.1.2.30
Alternative name(s):
17-alpha-hydroxyprogesterone aldolase
CYPXVII
Cytochrome P450 17A1
Cytochrome P450-C17
Short name=Cytochrome P450c17
Gene names
Name:Cyp17a1
Synonyms:Cyp17
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length507 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty.

Catalytic activity

A C(21)-steroid + (reduced NADPH--hemoprotein reductase) + O2 = a 17-alpha-hydroxy-C(21)-steroid + (oxidized NADPH--hemoprotein reductase) + H2O.

17-alpha-hydroxyprogesterone = androst-4-ene-3,17-dione + acetaldehyde.

Cofactor

Heme group By similarity.

Enzyme regulation

Regulated predominantly by intracellular cAMP levels.

Pathway

Lipid metabolism; steroid biosynthesis.

Subcellular location

Membrane Potential.

Sequence similarities

Belongs to the cytochrome P450 family.

Ontologies

Keywords
   Biological processSteroidogenesis
   Cellular componentMembrane
   LigandHeme
Iron
Metal-binding
   Molecular functionLyase
Monooxygenase
Oxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processLeydig cell differentiation

Inferred from electronic annotation. Source: Ensembl

adrenal gland development

Inferred from electronic annotation. Source: Ensembl

biphenyl metabolic process

Inferred from electronic annotation. Source: Ensembl

cellular response to antibiotic

Inferred from electronic annotation. Source: Ensembl

cellular response to gonadotropin stimulus

Inferred from electronic annotation. Source: Ensembl

cellular response to lipopolysaccharide

Inferred from electronic annotation. Source: Ensembl

dibenzo-p-dioxin metabolic process

Inferred from electronic annotation. Source: Ensembl

glucocorticoid biosynthetic process

Inferred from direct assay PubMed 7588219. Source: MGI

hippocampus development

Inferred from electronic annotation. Source: Ensembl

hormone biosynthetic process

Inferred from sequence or structural similarity. Source: UniProtKB

ovulation

Inferred from electronic annotation. Source: Ensembl

phenol-containing compound metabolic process

Inferred from electronic annotation. Source: Ensembl

phthalate metabolic process

Inferred from electronic annotation. Source: Ensembl

positive regulation of steroid hormone biosynthetic process

Inferred from electronic annotation. Source: Ensembl

progesterone metabolic process

Inferred from sequence or structural similarity. Source: UniProtKB

response to acetate

Inferred from electronic annotation. Source: Ensembl

response to cAMP

Inferred from electronic annotation. Source: Ensembl

response to cytokine

Inferred from electronic annotation. Source: Ensembl

response to drug

Inferred from electronic annotation. Source: Ensembl

response to fungicide

Inferred from electronic annotation. Source: Ensembl

response to herbicide

Inferred from electronic annotation. Source: Ensembl

response to insecticide

Inferred from electronic annotation. Source: Ensembl

response to ionizing radiation

Inferred from electronic annotation. Source: Ensembl

response to methylmercury

Inferred from electronic annotation. Source: Ensembl

response to nutrient levels

Inferred from electronic annotation. Source: Ensembl

response to retinoic acid

Inferred from electronic annotation. Source: Ensembl

response to steroid hormone

Inferred from electronic annotation. Source: Ensembl

steroid metabolic process

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentaxon

Inferred from direct assay PubMed 7588260. Source: MGI

endoplasmic reticulum

Inferred from electronic annotation. Source: Ensembl

membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

mitochondrion

Inferred from direct assay PubMed 14651853. Source: MGI

neuronal cell body

Inferred from direct assay PubMed 7588260. Source: MGI

   Molecular_function17-alpha-hydroxyprogesterone aldolase activity

Inferred from electronic annotation. Source: UniProtKB-EC

heme binding

Inferred from sequence or structural similarity. Source: UniProtKB

iron ion binding

Inferred from electronic annotation. Source: InterPro

steroid 17-alpha-monooxygenase activity

Inferred from direct assay PubMed 7588219. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 507507Steroid 17-alpha-hydroxylase/17,20 lyase
PRO_0000051935

Sites

Metal binding4411Iron (heme axial ligand) By similarity

Sequences

Sequence LengthMass (Da)Tools
P27786 [UniParc].

Last modified August 1, 1992. Version 1.
Checksum: C0916193356EFEBD

FASTA50757,638
        10         20         30         40         50         60 
MWELVGLLLL ILAYFFWPKS KTPNAKFPRS LPFLPLVGSL PFLPRRGHMH ANFFKLQEKY 

        70         80         90        100        110        120 
GPIYSLRLGT TTAVIVGHYQ LAREVLVKKG KEFSGRPQMV TLGLLSDQGK GVAFADSSSS 

       130        140        150        160        170        180 
WQLHRKLVFS TFSLFRDDQK LEKMICQEAN SLCDLILTYD GESRDLSTLI FKSVINIICT 

       190        200        210        220        230        240 
ICFNISFENK DPILTTIQTF TEGIVDVLGH SDLVDIFPWL KIFPNKNLEM IKEHTKIREK 

       250        260        270        280        290        300 
TLVEMFEKCK EKFNSESLSS LTDILIQAKM NAENNNTGEG QDPSVFSDKH ILVTVGDIFG 

       310        320        330        340        350        360 
AGIETTSSVL NWILAFLVHN PEVKRKIQKE IDQYVGFSRT PSFNDRTHLL MLEATIREVL 

       370        380        390        400        410        420 
RIRPVAPLLI PHKANIDSSI GEFAIPKDTH VIINLWALHH DKNEWDQPDR FMPERFLDPT 

       430        440        450        460        470        480 
GSHLITPTPS YLPFGAGPRS CIGEALARQE LFIFMALLLQ RFDFDVSDDK QLPCLVGDPK 

       490        500 
VVFLIDPFKV KITVRQAWKD AQVEVST 

« Hide

References

[1]"Isolation, characterization, and chromosomal mapping of mouse P450 17 alpha-hydroxylase/C17-20 lyase."
Youngblood G.L., Sartorius C., Taylor B.A., Payne A.H.
Genomics 10:270-275(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M64863 mRNA. Translation: AAA39877.1.
PIRA39072.
RefSeqNP_031835.3. NM_007809.3.
UniGeneMm.1262.

3D structure databases

ProteinModelPortalP27786.
SMRP27786. Positions 34-502.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteP27786.

Proteomic databases

PaxDbP27786.
PRIDEP27786.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000026012; ENSMUSP00000026012; ENSMUSG00000003555.
GeneID13074.
KEGGmmu:13074.
UCSCuc008hua.2. mouse.

Organism-specific databases

CTD1586.
MGIMGI:88586. Cyp17a1.

Phylogenomic databases

eggNOGCOG2124.
HOGENOMHOG000036991.
HOVERGENHBG106944.
InParanoidP27786.
KOK00512.
OMAILAYFFW.
OrthoDBEOG7RBZ85.
PhylomeDBP27786.
TreeFamTF105095.

Enzyme and pathway databases

UniPathwayUPA00062.

Gene expression databases

BgeeP27786.
GenevestigatorP27786.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio283012.
PROP27786.
SOURCESearch...

Entry information

Entry nameCP17A_MOUSE
AccessionPrimary (citable) accession number: P27786
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: April 16, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot