Reviewed, UniProtKB/Swiss-Prot P27783 (NIA_BETVE)

Names and origin

Protein names

Recommended name:
    Nitrate reductase [NAD(P)H]
      Short name=NR
    EC=1.7.1.2

Gene names

Name:

NIA1

Organism

Betula verrucosa (White birch) (Betula pendula)

Taxonomic identifier

3505 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fagales › Betulaceae › Betula

Protein attributes

Sequence length	898 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.
Catalytic activity	Nitrite + NAD(P)+ + H2O = nitrate + NAD(P)H.
Cofactor	Binds 1 FAD per subunit. Binds 1 heme group per subunit. Binds 1 molybdenum-pterin group per subunit.
Subunit structure	Homodimer.
Induction	By nitrate.
Sequence similarities	Belongs to the nitrate reductase family. Contains 1 cytochrome b5 heme-binding domain. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Biological process	Nitrate assimilation
Ligand	FAD Flavoprotein Heme Iron Metal-binding Molybdenum NAD NADP
Molecular function	Oxidoreductase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	nitrate assimilation Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro molybdenum ion binding Inferred from electronic annotation. Source: UniProtKB-KW nitrate reductase [NAD(P)H] activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 898	898	Nitrate reductase [NAD(P)H]		PRO_0000166075
Regions
	Domain	528 – 603	76	Cytochrome b5 heme-binding
	Domain	642 – 754	113	FAD-binding FR-type
Sites
	Metal binding	180	1	Molybdenum-pterin Potential
	Metal binding	234	1	Molybdenum-pterin Potential
	Metal binding	563	1	Iron (heme axial ligand) By similarity
	Metal binding	586	1	Iron (heme axial ligand) By similarity
Amino acid modifications
	Disulfide bond	419		Interchain Potential

Sequences

	Sequence		Length	Mass (Da)
	P27783-1 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: C6B2B2B12FBCDC58	FASTA	898	101,002
	10 20 30 40 50 60 MAASVENRRF THHEPAVNGL VRTFKPVPNS HRSDSPDLGR QIPSSPKKQV ATGEDSSSED 70 80 90 100 110 120 ENENDYKELI QKGNGELEPS ILDPRDEATA DNWVERNATM VRLTGKHPFN SEAPLTRLMH 130 140 150 160 170 180 HGFITPAPLH YVRNHGPVPK ARWEDWSVEV CGLVKRPARF TMDRLVTEFR SREFPVTLVC 190 200 210 220 230 240 AGNRRKEQNM VKKTIGFNWG AAGVSTSVWR GVPLRDVLKR CGIFSRGRGA FNVCFEGAED 250 260 270 280 290 300 LPGGGGSKYG TSVKYEMAMD PARDIILGYM QNGERLSPDH GFPVRMIIPG FIGGRMVKWL 310 320 330 340 350 360 KRIIVTTKES DNYYHYNDNR VLPSHVDADV AKAEAWWYKP EHIINELNIN SVITTPCHEE 370 380 390 400 410 420 ILPINSWTTQ RPYTLRGYAY SGGGRKVTRV EITMNGGEKW RVCALDHPEK PNKYGKYWCW 430 440 450 460 470 480 CFWSLEVEVL DLLGAKEIAV RAWDEAHNTQ PEKLIWNVMG MMNNCWFRVK TNVCKAHMGE 490 500 510 520 530 540 IGIAFEHPTV PGNESGGWMA REKNLETSSD ANQSLKKSVS SPFMNTSSKM FSMSEVKKHN 550 560 570 580 590 600 SAESAWIIVH GHIYDCTHFL KDHPGGADSI LINAGTDCTE EFDAIHSDKA KKMLEDYRIG 610 620 630 640 650 660 ELITTGYVSD SPNSTVHGAS NTSHLAPIKE IAPLRNVALI PGAKIPTKLV YKKSLSHDVR 670 680 690 700 710 720 LFRLALPSDD QVLGLPVGKH VFLCATIDDK LCMRAYTPTS TIDEVGYLDL VVKIYFKNSN 730 740 750 760 770 780 PRFPNGGLMS QHLDSLPIGS VLHVKGPLGH VEYTGRGNFL VHGEPKFAKR LAMVAGGTGI 790 800 810 820 830 840 TPIYQVIQAI LKDPEDETEM FVVYANRTED DILLREELDD WAKKHEKLKV WYVVKESKRE 850 860 870 880 890 GWEYSVGYIR ESILREHIPE GSDDVLALAC GAPSMIEEAV RLNLEKMNYD TKNSLIIF

References

[1]

"Sequence of a cDNA encoding the bi-specific NAD(P)H-nitrate reductase from the tree Betula pendula and identification of conserved protein regions." Friemann A., Brinkmann K., Hachtel W. Mol. Gen. Genet. 227:97-105(1991) [PubMed: 1675424] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Leaf.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X54097 mRNA. Translation: CAA38031.1.
PIR	RDBJNH. S15959.
3D structure databases
HSSP	HSSP built from PDB template 2CND based on UniProtKB P17571.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.7.1.2. 228863.
Family and domain databases
InterPro	IPR001199. Cyt_B5. IPR018506. Cyt_B5_heme-BS. IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR005066. MoCF_OxRdtse_dimer. IPR008335. Mopterin_OxRdtase_euk. IPR001834. NADH-Cyt_B5_reductase. IPR012137. Nitr_rd_NADH. IPR008333. OxRdtase_FAD-bd. IPR001433. OxRdtase_FAD/NAD_bd. IPR000572. OxRdtase_Mopterin-bd. [Graphical view]
Gene3D	G3DSA:3.10.120.10. Cyt_B5. 1 hit. G3DSA:2.60.40.650. MoCF_oxrdtse_dimer. 1 hit. G3DSA:3.90.420.10. Oxred_molyb_bd. 1 hit.
Pfam	PF00173. Cyt-b5. 1 hit. PF00970. FAD_binding_6. 1 hit. PF03404. Mo-co_dimer. 1 hit. PF00175. NAD_binding_1. 1 hit. PF00174. Oxidored_molyb. 1 hit. [Graphical view]
PIRSF	PIRSF000233. Nitr_rd_NADH. 1 hit.
PRINTS	PR00406. CYTB5RDTASE. PR00363. CYTOCHROMEB5. PR00407. EUMOPTERIN. PR00371. FPNCR.
ProDom	PD000612. Cyt_B5. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00191. CYTOCHROME_B5_1. 1 hit. PS50255. CYTOCHROME_B5_2. 1 hit. PS51384. FAD_FR. 1 hit. PS00559. MOLYBDOPTERIN_EUK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NIA_BETVE

Accession

Primary (citable) accession number: P27783

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	June 16, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)