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Protein

Protein disulfide-isomerase A3

Gene

Pdia3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Catalyzes the rearrangement of -S-S- bonds in proteins.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei57NucleophileBy similarity1
Sitei58Contributes to redox potential valueBy similarity1
Sitei59Contributes to redox potential valueBy similarity1
Active sitei60NucleophileBy similarity1
Sitei119Lowers pKa of C-terminal Cys of first active siteBy similarity1
Active sitei406NucleophileBy similarity1
Sitei407Contributes to redox potential valueBy similarity1
Sitei408Contributes to redox potential valueBy similarity1
Active sitei409NucleophileBy similarity1
Sitei471Lowers pKa of C-terminal Cys of second active siteBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Enzyme and pathway databases

BRENDAi5.3.4.1. 3474.
ReactomeiR-MMU-1236974. ER-Phagosome pathway.
R-MMU-901042. Calnexin/calreticulin cycle.
R-MMU-983170. Antigen Presentation: Folding, assembly and peptide loading of class I MHC.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein disulfide-isomerase A3 (EC:5.3.4.1)
Alternative name(s):
58 kDa glucose-regulated protein
58 kDa microsomal protein
Short name:
p58
Disulfide isomerase ER-60
Endoplasmic reticulum resident protein 57
Short name:
ER protein 57
Short name:
ERp57
Endoplasmic reticulum resident protein 60
Short name:
ER protein 60
Short name:
ERp60
Gene namesi
Name:Pdia3
Synonyms:Erp, Erp60, Grp58
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:95834. Pdia3.

Subcellular locationi

  • Endoplasmic reticulum By similarity
  • Endoplasmic reticulum lumen By similarity
  • Melanosome By similarity

GO - Cellular componenti

  • cell surface Source: MGI
  • endoplasmic reticulum Source: MGI
  • endoplasmic reticulum lumen Source: Reactome
  • extracellular exosome Source: MGI
  • focal adhesion Source: MGI
  • melanosome Source: UniProtKB-SubCell
  • myelin sheath Source: UniProtKB
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 242 PublicationsAdd BLAST24
ChainiPRO_000003422625 – 505Protein disulfide-isomerase A3Add BLAST481

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi57 ↔ 60Redox-activePROSITE-ProRule annotation
Disulfide bondi57Interchain (with C-118 in TAPBP); in linked formBy similarity
Modified residuei61N6-methyllysineBy similarity1
Disulfide bondi85 ↔ 92By similarity
Modified residuei129N6-succinyllysineCombined sources1
Modified residuei152N6-acetyllysineCombined sources1
Modified residuei218N6-succinyllysineCombined sources1
Modified residuei252N6-acetyllysineCombined sources1
Modified residuei319PhosphothreonineBy similarity1
Modified residuei362N6-acetyllysineCombined sources1
Disulfide bondi406 ↔ 409Redox-activePROSITE-ProRule annotation
Modified residuei494N6-acetyllysineCombined sources1

Post-translational modificationi

Phosphorylated.1 Publication

Keywords - PTMi

Acetylation, Disulfide bond, Methylation, Phosphoprotein

Proteomic databases

EPDiP27773.
MaxQBiP27773.
PaxDbiP27773.
PeptideAtlasiP27773.
PRIDEiP27773.
TopDownProteomicsiP27773.

2D gel databases

REPRODUCTION-2DPAGEIPI00230108.
P27773.
Q3TEI9.
Q8C2F4.
SWISS-2DPAGEP27773.

PTM databases

iPTMnetiP27773.
PhosphoSitePlusiP27773.
SwissPalmiP27773.

Expressioni

Tissue specificityi

In caput and cauda epididymal spermatozoa, detected in the acrosome and principal piece (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000027248.
CleanExiMM_PDIA3.
ExpressionAtlasiP27773. baseline and differential.
GenevisibleiP27773. MM.

Interactioni

Subunit structurei

Subunit of the TAP complex, also known as the peptide loading complex (PLC). Can form disulfide-linked heterodimers with TAPBP. Interacts with ERP27 and CANX (By similarity). Interacts with MZB1 in a calcium-dependent manner. Interacts with SERPINA2 and with the S and Z variants of SERPINA1 (By similarity). Interacts with ATP2A2 (PubMed:23395171).By similarity1 Publication

Protein-protein interaction databases

BioGridi200077. 3 interactors.
IntActiP27773. 9 interactors.
MINTiMINT-4590033.
STRINGi10090.ENSMUSP00000028683.

Structurei

3D structure databases

ProteinModelPortaliP27773.
SMRiP27773.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 133Thioredoxin 1PROSITE-ProRule annotationAdd BLAST109
Domaini343 – 485Thioredoxin 2PROSITE-ProRule annotationAdd BLAST143

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi502 – 505Prevents secretion from ERBy similarity4

Sequence similaritiesi

Belongs to the protein disulfide isomerase family.Curated
Contains 2 thioredoxin domains.PROSITE-ProRule annotation

Keywords - Domaini

Redox-active center, Repeat, Signal

Phylogenomic databases

eggNOGiKOG0190. Eukaryota.
COG0526. LUCA.
GeneTreeiENSGT00860000133691.
HOGENOMiHOG000162459.
HOVERGENiHBG005920.
InParanoidiP27773.
KOiK08056.
OMAiQINFAIA.
OrthoDBiEOG091G05J9.
PhylomeDBiP27773.
TreeFamiTF106382.

Family and domain databases

Gene3Di3.40.30.10. 3 hits.
InterProiIPR005788. Disulphide_isomerase.
IPR005792. Prot_disulphide_isomerase.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF00085. Thioredoxin. 2 hits.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 4 hits.
TIGRFAMsiTIGR01130. ER_PDI_fam. 1 hit.
TIGR01126. pdi_dom. 2 hits.
PROSITEiPS00194. THIOREDOXIN_1. 2 hits.
PS51352. THIOREDOXIN_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27773-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRFSCLALLP GVALLLASAR LAAASDVLEL TDENFESRVS DTGSAGLMLV
60 70 80 90 100
EFFAPWCGHC KRLAPEYEAA ATRLKGIVPL AKVDCTANTN TCNKYGVSGY
110 120 130 140 150
PTLKIFRDGE EAGAYDGPRT ADGIVSHLKK QAGPASVPLR TEEEFKKFIS
160 170 180 190 200
DKDASVVGFF RDLFSDGHSE FLKAASNLRD NYRFAHTNIE SLVKEYDDNG
210 220 230 240 250
EGITIFRPLH LANKFEDKTV AYTEKKMTSG KIKKFIQDSI FGLCPHMTED
260 270 280 290 300
NKDLIQGKDL LTAYYDVDYE KNAKGSNYWR NRVMMVAKKF LDAGHKLNFA
310 320 330 340 350
VASRKTFSHE LSDFGLESTT GEVPVVAIRT AKGEKFVMQE EFSRDGKALE
360 370 380 390 400
QFLQEYFDGN LKRYLKSEPI PESNEGPVKV VVAENFDDIV NEEDKDVLIE
410 420 430 440 450
FYAPWCGHCK NLEPKYKELG EKLSKDPNIV IAKMDATAND VPSPYEVKGF
460 470 480 490 500
PTIYFSPANK KLTPKKYEGG RELNDFISYL QREATNPPII QEEKPKKKKK

AQEDL
Length:505
Mass (Da):56,678
Last modified:May 1, 2007 - v2
Checksum:i3A7CD1C35981C4B3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti20R → C in BAC40527 (PubMed:16141072).Curated1
Sequence conflicti20R → C in BAE41259 (PubMed:16141072).Curated1
Sequence conflicti76Missing in AAA39944 (PubMed:2033248).Curated1
Sequence conflicti108D → A in AAA39944 (PubMed:2033248).Curated1
Sequence conflicti230G → A in AAA39944 (PubMed:2033248).Curated1
Sequence conflicti264Y → F in BAE39834 (PubMed:16141072).Curated1
Sequence conflicti315G → S in AAA39944 (PubMed:2033248).Curated1
Sequence conflicti387D → G in BAE39834 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73329 mRNA. Translation: AAA39944.1.
DQ000491 Genomic DNA. Translation: AAY16987.1.
AK088721 mRNA. Translation: BAC40527.1.
AK133799 mRNA. Translation: BAE21849.1.
AK167807 mRNA. Translation: BAE39834.1.
AK169611 mRNA. Translation: BAE41259.1.
AL845466 Genomic DNA. Translation: CAM24231.1.
BC003285 mRNA. Translation: AAH03285.1.
BC033439 mRNA. Translation: AAH33439.1.
CCDSiCCDS16643.1.
RefSeqiNP_031978.2. NM_007952.2.
UniGeneiMm.263177.

Genome annotation databases

EnsembliENSMUST00000028683; ENSMUSP00000028683; ENSMUSG00000027248.
GeneIDi14827.
KEGGimmu:14827.
UCSCiuc008lzb.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73329 mRNA. Translation: AAA39944.1.
DQ000491 Genomic DNA. Translation: AAY16987.1.
AK088721 mRNA. Translation: BAC40527.1.
AK133799 mRNA. Translation: BAE21849.1.
AK167807 mRNA. Translation: BAE39834.1.
AK169611 mRNA. Translation: BAE41259.1.
AL845466 Genomic DNA. Translation: CAM24231.1.
BC003285 mRNA. Translation: AAH03285.1.
BC033439 mRNA. Translation: AAH33439.1.
CCDSiCCDS16643.1.
RefSeqiNP_031978.2. NM_007952.2.
UniGeneiMm.263177.

3D structure databases

ProteinModelPortaliP27773.
SMRiP27773.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200077. 3 interactors.
IntActiP27773. 9 interactors.
MINTiMINT-4590033.
STRINGi10090.ENSMUSP00000028683.

PTM databases

iPTMnetiP27773.
PhosphoSitePlusiP27773.
SwissPalmiP27773.

2D gel databases

REPRODUCTION-2DPAGEIPI00230108.
P27773.
Q3TEI9.
Q8C2F4.
SWISS-2DPAGEP27773.

Proteomic databases

EPDiP27773.
MaxQBiP27773.
PaxDbiP27773.
PeptideAtlasiP27773.
PRIDEiP27773.
TopDownProteomicsiP27773.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028683; ENSMUSP00000028683; ENSMUSG00000027248.
GeneIDi14827.
KEGGimmu:14827.
UCSCiuc008lzb.1. mouse.

Organism-specific databases

CTDi2923.
MGIiMGI:95834. Pdia3.

Phylogenomic databases

eggNOGiKOG0190. Eukaryota.
COG0526. LUCA.
GeneTreeiENSGT00860000133691.
HOGENOMiHOG000162459.
HOVERGENiHBG005920.
InParanoidiP27773.
KOiK08056.
OMAiQINFAIA.
OrthoDBiEOG091G05J9.
PhylomeDBiP27773.
TreeFamiTF106382.

Enzyme and pathway databases

BRENDAi5.3.4.1. 3474.
ReactomeiR-MMU-1236974. ER-Phagosome pathway.
R-MMU-901042. Calnexin/calreticulin cycle.
R-MMU-983170. Antigen Presentation: Folding, assembly and peptide loading of class I MHC.

Miscellaneous databases

ChiTaRSiPdia3. mouse.
PROiP27773.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027248.
CleanExiMM_PDIA3.
ExpressionAtlasiP27773. baseline and differential.
GenevisibleiP27773. MM.

Family and domain databases

Gene3Di3.40.30.10. 3 hits.
InterProiIPR005788. Disulphide_isomerase.
IPR005792. Prot_disulphide_isomerase.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF00085. Thioredoxin. 2 hits.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 4 hits.
TIGRFAMsiTIGR01130. ER_PDI_fam. 1 hit.
TIGR01126. pdi_dom. 2 hits.
PROSITEiPS00194. THIOREDOXIN_1. 2 hits.
PS51352. THIOREDOXIN_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPDIA3_MOUSE
AccessioniPrimary (citable) accession number: P27773
Secondary accession number(s): Q3TEI9
, Q3TIL2, Q3UZK8, Q8C2F4, Q99LF6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: May 1, 2007
Last modified: November 30, 2016
This is version 167 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to be a phosphatidylinositol 4,5-bisphosphate phosphodiesterase type I (phospholipase C-alpha).1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.