Reviewed, UniProtKB/Swiss-Prot P27773 (PDIA3_MOUSE)

Names and origin

Protein names

Recommended name:
    Protein disulfide-isomerase A3
    EC=5.3.4.1
Alternative name(s):
    Disulfide isomerase ER-60
    ERp60
    58 kDa microsomal protein
    p58
    ERp57

Gene names

Name:	Pdia3
Synonyms:	Erp, Erp60, Grp58

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	505 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Catalyzes the rearrangement of -S-S- bonds in proteins.
Subunit structure	Interacts with ERP27 and CANX By similarity.
Subcellular location	Endoplasmic reticulum lumen By similarity. Melanosome By similarity.
Post-translational modification	Phosphorylated. Ref.7
Sequence similarities	Belongs to the protein disulfide isomerase family. Contains 2 thioredoxin domains.
Caution	Was originally (Ref.1) thought to be a phosphatidylinositol-4,5-bisphosphate phosphodiesterase type I (phospholipase C-alpha).

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Redox-active center Repeat Signal
Molecular function	Isomerase
PTM	Disulfide bond Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro positive regulation of apoptosis Inferred from mutant phenotype. Source: MGI
Cellular component	endoplasmic reticulum Inferred from direct assay. Source: MGI endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell melanosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein disulfide isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Ref.7 Ref.6
	Chain	25 – 505	481	Protein disulfide-isomerase A3		PRO_0000034226
Regions
	Domain	25 – 133	109	Thioredoxin 1
	Domain	343 – 485	143	Thioredoxin 2
	Motif	502 – 505	4	Prevents secretion from ER By similarity
Sites
	Active site	57	1	Nucleophile By similarity
	Active site	60	1	Nucleophile By similarity
	Active site	406	1	Nucleophile By similarity
	Active site	409	1	Nucleophile By similarity
	Site	58	1	Contributes to redox potential value By similarity
	Site	59	1	Contributes to redox potential value By similarity
	Site	119	1	Lowers pKa of C-terminal Cys of first active site By similarity
	Site	407	1	Contributes to redox potential value By similarity
	Site	408	1	Contributes to redox potential value By similarity
	Site	471	1	Lowers pKa of C-terminal Cys of second active site By similarity
Amino acid modifications
	Disulfide bond	57 ↔ 60		Redox-active By similarity
	Disulfide bond	406 ↔ 409		Redox-active By similarity
Experimental info
	Sequence conflict	20	1	R → C in BAC40527. Ref.3
	Sequence conflict	20	1	R → C in BAE41259. Ref.3
	Sequence conflict	76	1	Missing in AAA39944. Ref.1
	Sequence conflict	108	1	D → A in AAA39944. Ref.1
	Sequence conflict	230	1	G → A in AAA39944. Ref.1
	Sequence conflict	264	1	Y → F in BAE39834. Ref.3
	Sequence conflict	315	1	G → S in AAA39944. Ref.1
	Sequence conflict	387	1	D → G in BAE39834. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P27773-1 [UniParc]. Last modified May 1, 2007. Version 2. Checksum: 3A7CD1C35981C4B3	FASTA	505	56,678
	10 20 30 40 50 60 MRFSCLALLP GVALLLASAR LAAASDVLEL TDENFESRVS DTGSAGLMLV EFFAPWCGHC 70 80 90 100 110 120 KRLAPEYEAA ATRLKGIVPL AKVDCTANTN TCNKYGVSGY PTLKIFRDGE EAGAYDGPRT 130 140 150 160 170 180 ADGIVSHLKK QAGPASVPLR TEEEFKKFIS DKDASVVGFF RDLFSDGHSE FLKAASNLRD 190 200 210 220 230 240 NYRFAHTNIE SLVKEYDDNG EGITIFRPLH LANKFEDKTV AYTEKKMTSG KIKKFIQDSI 250 260 270 280 290 300 FGLCPHMTED NKDLIQGKDL LTAYYDVDYE KNAKGSNYWR NRVMMVAKKF LDAGHKLNFA 310 320 330 340 350 360 VASRKTFSHE LSDFGLESTT GEVPVVAIRT AKGEKFVMQE EFSRDGKALE QFLQEYFDGN 370 380 390 400 410 420 LKRYLKSEPI PESNEGPVKV VVAENFDDIV NEEDKDVLIE FYAPWCGHCK NLEPKYKELG 430 440 450 460 470 480 EKLSKDPNIV IAKMDATAND VPSPYEVKGF PTIYFSPANK KLTPKKYEGG RELNDFISYL 490 500 QREATNPPII QEEKPKKKKK AQEDL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression of phospholipase C isozymes by murine B lymphocytes." Hempel W.M., Defranco A.L. J. Immunol. 146:3713-3720(1991) [PubMed: 2033248] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Mouse GRP58 gene structure." Wang W., Jaiswal A.K. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: C57BL/6.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J, DBA/2 and NOD. Tissue: Thymus.
[4]	The mouse genome sequencing consortium Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Czech II and FVB/N. Tissue: Mammary tumor.
[6]	"Separation and sequencing of familiar and novel murine proteins using preparative two-dimensional gel electrophoresis." Merrick B.A., Patterson R.M., Wichter L.L., He C., Selkirk J.K. Electrophoresis 15:735-745(1994) [PubMed: 7523108] [Abstract] Cited for: PROTEIN SEQUENCE OF 25-41 AND 306-329. Tissue: Fibroblast.
[7]	"Identification of the two isoforms of phospholipase C-alpha from dividing murine fibroblasts by protein microsequencing." Merrick B.A., Wichter L.L., Patterson R.M., He C., Selkirk J.K. Biochem. Arch. 9:335-340(1993) Cited for: PROTEIN SEQUENCE OF 25-41, PHOSPHORYLATION. Tissue: Fibroblast.
[8]	Lubec G., Kang S.U., Klug S., Yang J.W., Zigmond M. Submitted (JUL-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 63-73; 94-103; 119-128; 130-139; 147-172; 183-193; 195-214; 234-251; 258-270; 306-329; 335-343; 347-362; 366-378; 448-459 AND 472-495, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain and Hippocampus.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M73329 mRNA. Translation: AAA39944.1. DQ000491 Genomic DNA. Translation: AAY16987.1. AK088721 mRNA. Translation: BAC40527.1. AK133799 mRNA. Translation: BAE21849.1. AK167807 mRNA. Translation: BAE39834.1. AK169611 mRNA. Translation: BAE41259.1. AL845466 Genomic DNA. Translation: CAM24231.1. BC003285 mRNA. Translation: AAH03285.1. BC033439 mRNA. Translation: AAH33439.1.
IPI	IPI00230108.
RefSeq	NP_031978.2.
UniGene	Mm.263177
3D structure databases
HSSP	HSSP built from PDB template 1MEK based on UniProtKB P07237.
SMR	P27773. Positions 25-137, 134-365, 357-488.
ModBase	Search...
PTM databases
PhosphoSite	P27773.
2-D gel databases
SWISS-2DPAGE	P27773.
REPRODUCTION-2DPAGE	P27773. Q3TEI9. Q8C2F4.
Proteomic databases
PRIDE	P27773.
Genome annotation databases
Ensembl	ENSMUSG00000027248. Mus musculus. [Contig view]
GeneID	14827.
KEGG	mmu:14827.
Organism-specific databases
MGI	MGI:95834. Pdia3.
Phylogenomic databases
HOGENOM	P27773.
HOVERGEN	P27773.
OMA	P27773. EGITLFR.
Enzyme and pathway databases
BRENDA	5.3.4.1. 244.
Gene expression databases
ArrayExpress	P27773.
Bgee	P27773.
CleanEx	MM_PDIA3.
GermOnline	ENSMUSG00000027248. Mus musculus.
Family and domain databases
InterPro	IPR005788. Disulphide_isomerase. IPR005792. Prot_disulphide_isomerase. IPR017936. Thioredoxin-like. IPR006662. Thioredoxin-like_subdom. IPR017937. Thioredoxin_CS. IPR013766. Thioredoxin_domain. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
Pfam	PF00085. Thioredoxin. 2 hits. [Graphical view]
PRINTS	PR00421. THIOREDOXIN.
TIGRFAMs	TIGR01130. ER_PDI_fam. 1 hit. TIGR01126. pdi_dom. 2 hits.
PROSITE	PS00194. THIOREDOXIN_1. 2 hits. PS51352. THIOREDOXIN_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	287035.
SOURCE	Search...

Entry information

Entry name

PDIA3_MOUSE

Accession

Primary (citable) accession number: P27773
Secondary accession number(s): Q3TEI9 , Q3TIL2, Q3UZK8, Q8C2F4, Q99LF6

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	May 1, 2007
Last modified:	June 16, 2009
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)