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P27762

- PLY4_AMBAR

UniProt

P27762 - PLY4_AMBAR

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Protein

Pectate lyase 4

Gene
N/A
Organism
Ambrosia artemisiifolia (Short ragweed)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Has pectate lyase activity.By similarity

Catalytic activityi

Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.

Cofactori

Ca2+By similarityNote: Binds 1 Ca(2+) ion.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi194 – 1941CalciumBy similarity
Metal bindingi218 – 2181CalciumBy similarity
Metal bindingi222 – 2221CalciumBy similarity
Active sitei274 – 2741Sequence Analysis

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. pectate lyase activity Source: UniProtKB-EC

GO - Biological processi

  1. pectin catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00545; UER00824.

Protein family/group databases

CAZyiPL1. Polysaccharide Lyase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Pectate lyase 4 (EC:4.2.2.2)
Alternative name(s):
Antigen Amb a II
Antigen K
Short name:
AgK
Pollen allergen Amb a 2
Allergen: Amb a 2
OrganismiAmbrosia artemisiifolia (Short ragweed)
Taxonomic identifieri4212 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridscampanulidsAsteralesAsteraceaeAsteroideaeHeliantheae allianceHeliantheaeAmbrosia

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human. This is one of the major allergens of the ragweed pollen.1 Publication

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei26. Amb a 1.0501.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence AnalysisAdd
BLAST
Chaini26 – 397372Pectate lyase 4PRO_0000024905Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi36 – 361N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi54 ↔ 71By similarity

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

Pollen and flowers.

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliP27762.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati159 – 20244PbH1 1Add
BLAST
Repeati203 – 22422PbH1 2Add
BLAST
Repeati227 – 24822PbH1 3Add
BLAST

Sequence similaritiesi

Contains 3 PbH1 repeats.Curated

Keywords - Domaini

Repeat, Signal

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR002022. Amb_allergen_dom.
IPR018082. AmbAllergen.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00544. Pec_lyase_C. 1 hit.
[Graphical view]
PRINTSiPR00807. AMBALLERGEN.
SMARTiSM00656. Amb_all. 1 hit.
SM00710. PbH1. 3 hits.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27762-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGIKHCCYIL YFTLALVTLV QAGRLGEEVD ILPSPNDTRR SLQGCEAHNI
60 70 80 90 100
IDKCWRCKPD WAENRQALGN CAQGFGKATH GGKWGDIYMV TSDQDDDVVN
110 120 130 140 150
PKEGTLRFGA TQDRPLWIIF QRDMIIYLQQ EMVVTSDKTI DGRGAKVELV
160 170 180 190 200
YGGITLMNVK NVIIHNIDIH DVRVLPGGRI KSNGGPAIPR HQSDGDAIHV
210 220 230 240 250
TGSSDIWIDH CTLSKSFDGL VDVNWGSTGV TISNCKFTHH EKAVLLGASD
260 270 280 290 300
THFQDLKMHV TLAYNIFTNT VHERMPRCRF GFFQIVNNFY DRWDKYAIGG
310 320 330 340 350
SSNPTILSQG NKFVAPDFIY KKNVCLRTGA QEPEWMTWNW RTQNDVLENG
360 370 380 390
AIFVASGSDP VLTAEQNAGM MQAEPGDMVP QLTMNAGVLT CSPGAPC
Length:397
Mass (Da):44,082
Last modified:August 1, 1992 - v1
Checksum:iC78617E4C9A3D1DD
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti70 – 701N → D Detected only in flower DNA.
Natural varianti138 – 1381K → T Detected only in flower DNA.
Natural varianti321 – 3211K → R Detected only in flower DNA.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80561 mRNA. Translation: AAA32671.1.
PIRiE53240.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80561 mRNA. Translation: AAA32671.1 .
PIRi E53240.

3D structure databases

ProteinModelPortali P27762.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

Allergomei 26. Amb a 1.0501.
CAZyi PL1. Polysaccharide Lyase Family 1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00545 ; UER00824 .

Family and domain databases

Gene3Di 2.160.20.10. 1 hit.
InterProi IPR002022. Amb_allergen_dom.
IPR018082. AmbAllergen.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view ]
Pfami PF00544. Pec_lyase_C. 1 hit.
[Graphical view ]
PRINTSi PR00807. AMBALLERGEN.
SMARTi SM00656. Amb_all. 1 hit.
SM00710. PbH1. 3 hits.
[Graphical view ]
SUPFAMi SSF51126. SSF51126. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of the allergen Amb alpha II. Recombinant expression and reactivity with T cells from ragweed allergic patients."
    Rogers B.L., Morgenstern J.P., Griffith I.J., Yu X.-B., Counsell C.M., Brauer A.W., King T.P., Garman R.D., Kuo M.-C.C.
    J. Immunol. 147:2547-2552(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALLERGEN.
    Tissue: Flower.
  2. "Sequence polymorphism of Amb a I and Amb a II, the major allergens in Ambrosia artemisiifolia (short ragweed)."
    Griffith I.J., Pollock J., Klapper D.G., Rogers B.L., Nault A.K.
    Int. Arch. Allergy Appl. Immunol. 96:296-304(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS.
    Tissue: Pollen.

Entry informationi

Entry nameiPLY4_AMBAR
AccessioniPrimary (citable) accession number: P27762
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: November 26, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3