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P27762

- PLY4_AMBAR

UniProt

P27762 - PLY4_AMBAR

Protein

Pectate lyase 4

Gene
N/A
Organism
Ambrosia artemisiifolia (Short ragweed)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (01 Aug 1992)
      Previous versions | rss
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    Functioni

    Has pectate lyase activity.By similarity

    Catalytic activityi

    Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.

    Cofactori

    Binds 1 calcium ion.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi194 – 1941CalciumBy similarity
    Metal bindingi218 – 2181CalciumBy similarity
    Metal bindingi222 – 2221CalciumBy similarity
    Active sitei274 – 2741Sequence Analysis

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. pectate lyase activity Source: UniProtKB-EC

    GO - Biological processi

    1. pectin catabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00545; UER00824.

    Protein family/group databases

    CAZyiPL1. Polysaccharide Lyase Family 1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pectate lyase 4 (EC:4.2.2.2)
    Alternative name(s):
    Antigen Amb a II
    Antigen K
    Short name:
    AgK
    Pollen allergen Amb a 2
    Allergen: Amb a 2
    OrganismiAmbrosia artemisiifolia (Short ragweed)
    Taxonomic identifieri4212 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridscampanulidsAsteralesAsteraceaeAsteroideaeHeliantheae allianceHeliantheaeAmbrosia

    Pathology & Biotechi

    Allergenic propertiesi

    Causes an allergic reaction in human. This is one of the major allergens of the ragweed pollen.1 Publication

    Keywords - Diseasei

    Allergen

    Protein family/group databases

    Allergomei26. Amb a 1.0501.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2525Sequence AnalysisAdd
    BLAST
    Chaini26 – 397372Pectate lyase 4PRO_0000024905Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi36 – 361N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi54 ↔ 71By similarity

    Post-translational modificationi

    The N-terminus is blocked.

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Expressioni

    Tissue specificityi

    Pollen and flowers.

    Interactioni

    Subunit structurei

    Monomer.

    Structurei

    3D structure databases

    ProteinModelPortaliP27762.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati159 – 20244PbH1 1Add
    BLAST
    Repeati203 – 22422PbH1 2Add
    BLAST
    Repeati227 – 24822PbH1 3Add
    BLAST

    Sequence similaritiesi

    Contains 3 PbH1 repeats.Curated

    Keywords - Domaini

    Repeat, Signal

    Family and domain databases

    Gene3Di2.160.20.10. 1 hit.
    InterProiIPR002022. Amb_allergen_dom.
    IPR018082. AmbAllergen.
    IPR006626. PbH1.
    IPR012334. Pectin_lyas_fold.
    IPR011050. Pectin_lyase_fold/virulence.
    [Graphical view]
    PfamiPF00544. Pec_lyase_C. 1 hit.
    [Graphical view]
    PRINTSiPR00807. AMBALLERGEN.
    SMARTiSM00656. Amb_all. 1 hit.
    SM00710. PbH1. 3 hits.
    [Graphical view]
    SUPFAMiSSF51126. SSF51126. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P27762-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGIKHCCYIL YFTLALVTLV QAGRLGEEVD ILPSPNDTRR SLQGCEAHNI    50
    IDKCWRCKPD WAENRQALGN CAQGFGKATH GGKWGDIYMV TSDQDDDVVN 100
    PKEGTLRFGA TQDRPLWIIF QRDMIIYLQQ EMVVTSDKTI DGRGAKVELV 150
    YGGITLMNVK NVIIHNIDIH DVRVLPGGRI KSNGGPAIPR HQSDGDAIHV 200
    TGSSDIWIDH CTLSKSFDGL VDVNWGSTGV TISNCKFTHH EKAVLLGASD 250
    THFQDLKMHV TLAYNIFTNT VHERMPRCRF GFFQIVNNFY DRWDKYAIGG 300
    SSNPTILSQG NKFVAPDFIY KKNVCLRTGA QEPEWMTWNW RTQNDVLENG 350
    AIFVASGSDP VLTAEQNAGM MQAEPGDMVP QLTMNAGVLT CSPGAPC 397
    Length:397
    Mass (Da):44,082
    Last modified:August 1, 1992 - v1
    Checksum:iC78617E4C9A3D1DD
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti70 – 701N → D Detected only in flower DNA.
    Natural varianti138 – 1381K → T Detected only in flower DNA.
    Natural varianti321 – 3211K → R Detected only in flower DNA.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M80561 mRNA. Translation: AAA32671.1.
    PIRiE53240.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M80561 mRNA. Translation: AAA32671.1 .
    PIRi E53240.

    3D structure databases

    ProteinModelPortali P27762.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    Allergomei 26. Amb a 1.0501.
    CAZyi PL1. Polysaccharide Lyase Family 1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00545 ; UER00824 .

    Family and domain databases

    Gene3Di 2.160.20.10. 1 hit.
    InterProi IPR002022. Amb_allergen_dom.
    IPR018082. AmbAllergen.
    IPR006626. PbH1.
    IPR012334. Pectin_lyas_fold.
    IPR011050. Pectin_lyase_fold/virulence.
    [Graphical view ]
    Pfami PF00544. Pec_lyase_C. 1 hit.
    [Graphical view ]
    PRINTSi PR00807. AMBALLERGEN.
    SMARTi SM00656. Amb_all. 1 hit.
    SM00710. PbH1. 3 hits.
    [Graphical view ]
    SUPFAMi SSF51126. SSF51126. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of the allergen Amb alpha II. Recombinant expression and reactivity with T cells from ragweed allergic patients."
      Rogers B.L., Morgenstern J.P., Griffith I.J., Yu X.-B., Counsell C.M., Brauer A.W., King T.P., Garman R.D., Kuo M.-C.C.
      J. Immunol. 147:2547-2552(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALLERGEN.
      Tissue: Flower.
    2. "Sequence polymorphism of Amb a I and Amb a II, the major allergens in Ambrosia artemisiifolia (short ragweed)."
      Griffith I.J., Pollock J., Klapper D.G., Rogers B.L., Nault A.K.
      Int. Arch. Allergy Appl. Immunol. 96:296-304(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS.
      Tissue: Pollen.

    Entry informationi

    Entry nameiPLY4_AMBAR
    AccessioniPrimary (citable) accession number: P27762
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: August 1, 1992
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. Allergens
      Nomenclature of allergens and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3