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P27762 (PLY4_AMBAR) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pectate lyase 4

EC=4.2.2.2
Alternative name(s):
Antigen Amb a II
Antigen K
Short name=AgK
Pollen allergen Amb a 2
Allergen=Amb a 2
OrganismAmbrosia artemisiifolia (Short ragweed)
Taxonomic identifier4212 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridscampanulidsAsteralesAsteraceaeAsteroideaeHeliantheae allianceHeliantheaeAmbrosia

Protein attributes

Sequence length397 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Has pectate lyase activity By similarity.

Catalytic activity

Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.

Cofactor

Binds 1 calcium ion By similarity.

Pathway

Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 2/5.

Subunit structure

Monomer.

Tissue specificity

Pollen and flowers.

Post-translational modification

The N-terminus is blocked.

Allergenic properties

Causes an allergic reaction in human. This is one of the major allergens of the ragweed pollen. Ref.1

Sequence similarities

Belongs to the polysaccharide lyase 1 family. Amb a subfamily.

Contains 3 PbH1 repeats.

Ontologies

Keywords
   Coding sequence diversityPolymorphism
   DiseaseAllergen
   DomainRepeat
Signal
   LigandCalcium
Metal-binding
   Molecular functionLyase
   PTMDisulfide bond
Glycoprotein
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Chain26 – 397372Pectate lyase 4
PRO_0000024905

Regions

Repeat159 – 20244PbH1 1
Repeat203 – 22422PbH1 2
Repeat227 – 24822PbH1 3

Sites

Active site2741 Potential
Metal binding1941Calcium By similarity
Metal binding2181Calcium By similarity
Metal binding2221Calcium By similarity

Amino acid modifications

Glycosylation361N-linked (GlcNAc...) Potential
Disulfide bond54 ↔ 71 By similarity

Natural variations

Natural variant701N → D Detected only in flower DNA.
Natural variant1381K → T Detected only in flower DNA.
Natural variant3211K → R Detected only in flower DNA.

Sequences

Sequence LengthMass (Da)Tools
P27762 [UniParc].

Last modified August 1, 1992. Version 1.
Checksum: C78617E4C9A3D1DD

FASTA39744,082
        10         20         30         40         50         60 
MGIKHCCYIL YFTLALVTLV QAGRLGEEVD ILPSPNDTRR SLQGCEAHNI IDKCWRCKPD 

        70         80         90        100        110        120 
WAENRQALGN CAQGFGKATH GGKWGDIYMV TSDQDDDVVN PKEGTLRFGA TQDRPLWIIF 

       130        140        150        160        170        180 
QRDMIIYLQQ EMVVTSDKTI DGRGAKVELV YGGITLMNVK NVIIHNIDIH DVRVLPGGRI 

       190        200        210        220        230        240 
KSNGGPAIPR HQSDGDAIHV TGSSDIWIDH CTLSKSFDGL VDVNWGSTGV TISNCKFTHH 

       250        260        270        280        290        300 
EKAVLLGASD THFQDLKMHV TLAYNIFTNT VHERMPRCRF GFFQIVNNFY DRWDKYAIGG 

       310        320        330        340        350        360 
SSNPTILSQG NKFVAPDFIY KKNVCLRTGA QEPEWMTWNW RTQNDVLENG AIFVASGSDP 

       370        380        390 
VLTAEQNAGM MQAEPGDMVP QLTMNAGVLT CSPGAPC 

« Hide

References

[1]"Complete sequence of the allergen Amb alpha II. Recombinant expression and reactivity with T cells from ragweed allergic patients."
Rogers B.L., Morgenstern J.P., Griffith I.J., Yu X.-B., Counsell C.M., Brauer A.W., King T.P., Garman R.D., Kuo M.-C.C.
J. Immunol. 147:2547-2552(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALLERGEN.
Tissue: Flower.
[2]"Sequence polymorphism of Amb a I and Amb a II, the major allergens in Ambrosia artemisiifolia (short ragweed)."
Griffith I.J., Pollock J., Klapper D.G., Rogers B.L., Nault A.K.
Int. Arch. Allergy Appl. Immunol. 96:296-304(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS.
Tissue: Pollen.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M80561 mRNA. Translation: AAA32671.1.
PIRE53240.

3D structure databases

ProteinModelPortalP27762.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

Allergome26. Amb a 1.0501.
CAZyPL1. Polysaccharide Lyase Family 1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00545; UER00824.

Family and domain databases

Gene3D2.160.20.10. 1 hit.
InterProIPR002022. Amb_allergen_dom.
IPR018082. AmbAllergen.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamPF00544. Pec_lyase_C. 1 hit.
[Graphical view]
PRINTSPR00807. AMBALLERGEN.
SMARTSM00656. Amb_all. 1 hit.
SM00710. PbH1. 3 hits.
[Graphical view]
SUPFAMSSF51126. SSF51126. 1 hit.
ProtoNetSearch...

Entry information

Entry namePLY4_AMBAR
AccessionPrimary (citable) accession number: P27762
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: April 16, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Allergens

Nomenclature of allergens and list of entries