P27762 (MPAA2_AMBAR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Pollen allergen Amb a 2 Alternative name(s): Antigen Amb a II Antigen K Short name=AgK Allergen=Amb a 2
Organism	Ambrosia artemisiifolia (Short ragweed)
Taxonomic identifier	4212 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › campanulids › Asterales › Asteraceae › Asteroideae › Heliantheae alliance › Heliantheae › Ambrosia

Protein attributes

Sequence length	397 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Monomer.
Tissue specificity	Pollen and flowers.
Post-translational modification	The N-terminus is blocked.
Allergenic properties	Causes an allergic reaction in human. This is one of the major allergens of the ragweed pollen. Ref.1
Sequence similarities	Belongs to the polysaccharide lyase 1 family. Amb a subfamily.

Ontologies

Keywords
Coding sequence diversity	Polymorphism
Disease	Allergen
Domain	Signal
PTM	Disulfide bond
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 397	372	Pollen allergen Amb a 2		PRO_0000024905
Amino acid modifications
	Disulfide bond	54 ↔ 71		By similarity
Natural variations
	Natural variant	70	1	N → D Detected only in flower DNA.
	Natural variant	138	1	K → T Detected only in flower DNA.
	Natural variant	321	1	K → R Detected only in flower DNA.

Sequences

	Sequence		Length	Mass (Da)
	P27762 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: C78617E4C9A3D1DD	FASTA	397	44,082
	10 20 30 40 50 60 MGIKHCCYIL YFTLALVTLV QAGRLGEEVD ILPSPNDTRR SLQGCEAHNI IDKCWRCKPD 70 80 90 100 110 120 WAENRQALGN CAQGFGKATH GGKWGDIYMV TSDQDDDVVN PKEGTLRFGA TQDRPLWIIF 130 140 150 160 170 180 QRDMIIYLQQ EMVVTSDKTI DGRGAKVELV YGGITLMNVK NVIIHNIDIH DVRVLPGGRI 190 200 210 220 230 240 KSNGGPAIPR HQSDGDAIHV TGSSDIWIDH CTLSKSFDGL VDVNWGSTGV TISNCKFTHH 250 260 270 280 290 300 EKAVLLGASD THFQDLKMHV TLAYNIFTNT VHERMPRCRF GFFQIVNNFY DRWDKYAIGG 310 320 330 340 350 360 SSNPTILSQG NKFVAPDFIY KKNVCLRTGA QEPEWMTWNW RTQNDVLENG AIFVASGSDP 370 380 390 VLTAEQNAGM MQAEPGDMVP QLTMNAGVLT CSPGAPC

References

[1]	"Complete sequence of the allergen Amb alpha II. Recombinant expression and reactivity with T cells from ragweed allergic patients." Rogers B.L., Morgenstern J.P., Griffith I.J., Yu X.-B., Counsell C.M., Brauer A.W., King T.P., Garman R.D., Kuo M.-C.C. J. Immunol. 147:2547-2552(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALLERGEN. Tissue: Flower.
[2]	"Sequence polymorphism of Amb a I and Amb a II, the major allergens in Ambrosia artemisiifolia (short ragweed)." Griffith I.J., Pollock J., Klapper D.G., Rogers B.L., Nault A.K. Int. Arch. Allergy Appl. Immunol. 96:296-304(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS. Tissue: Pollen.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M80561 mRNA. Translation: AAA32671.1.
PIR	E53240.
3D structure databases
ProteinModelPortal	P27762.
ModBase	Search...
Protein family/group databases
Allergome	26. Amb a 1.0501.
CAZy	PL1. Polysaccharide Lyase Family 1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	2.160.20.10. 1 hit.
InterPro	IPR002022. Amb_allergen_dom. IPR018082. AmbAllergen. IPR006626. PbH1. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Pfam	PF00544. Pec_lyase_C. 1 hit. [Graphical view]
PRINTS	PR00807. AMBALLERGEN.
SMART	SM00656. Amb_all. 1 hit. SM00710. PbH1. 3 hits. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MPAA2_AMBAR

Accession

Primary (citable) accession number: P27762

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	April 3, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program