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P27761 (PLY2_AMBAR) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pectate lyase 2

EC=4.2.2.2
Alternative name(s):
Antigen Amb a I
Antigen E
Short name=AgE
Pollen allergen Amb a 1.3
Allergen=Amb a 1.3
OrganismAmbrosia artemisiifolia (Short ragweed)
Taxonomic identifier4212 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridscampanulidsAsteralesAsteraceaeAsteroideaeHeliantheae allianceHeliantheaeAmbrosia

Protein attributes

Sequence length397 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Has pectate lyase activity By similarity.

Catalytic activity

Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.

Cofactor

Binds 1 calcium ion By similarity.

Pathway

Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 2/5.

Subunit structure

Monomer.

Tissue specificity

Pollen and flowers.

Post-translational modification

The N-terminus is blocked.

Allergenic properties

Causes an allergic reaction in human. This is one of the major allergens of the ragweed pollen. Ref.1

Sequence similarities

Belongs to the polysaccharide lyase 1 family. Amb a subfamily.

Ontologies

Keywords
   Coding sequence diversityPolymorphism
   DiseaseAllergen
   DomainSignal
   LigandCalcium
Metal-binding
   Molecular functionLyase
   PTMDisulfide bond
Glycoprotein
Gene Ontology (GO)
   Biological_processpectin catabolic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

pectate lyase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Chain26 – 397372Pectate lyase 2
PRO_0000024903

Sites

Active site2741 Potential
Metal binding1941Calcium By similarity
Metal binding2181Calcium By similarity
Metal binding2221Calcium By similarity

Amino acid modifications

Glycosylation371N-linked (GlcNAc...) Potential
Disulfide bond54 ↔ 71 By similarity

Natural variations

Natural variant481L → Y.

Sequences

Sequence LengthMass (Da)Tools
P27761 [UniParc].

Last modified August 1, 1992. Version 1.
Checksum: C8DB41257590DD0A

FASTA39742,928
        10         20         30         40         50         60 
MGIKQCCYIL YFTLALVALL QPVRSAEGVG EILPSVNETR SLQACEALNI IDKCWRGKAD 

        70         80         90        100        110        120 
WENNRQALAD CAQGFAKGTY GGKWGDVYTV TSNLDDDVAN PKEGTLRFAA AQNRPLWIIF 

       130        140        150        160        170        180 
KNDMVINLNQ ELVVNSDKTI DGRGVKVEII NGGLTLMNVK NIIIHNINIH DVKVLPGGMI 

       190        200        210        220        230        240 
KSNDGPPILR QASDGDTINV AGSSQIWIDH CSLSKSFDGL VDVTLGSTHV TISNCKFTQQ 

       250        260        270        280        290        300 
SKAILLGADD THVQDKGMLA TVAFNMFTDN VDQRMPRCRF GFFQVVNNNY DRWGTYAIGG 

       310        320        330        340        350        360 
SSAPTILCQG NRFLAPDDQI KKNVLARTGT GAAESMAWNW RSDKDLLENG AIFVTSGSDP 

       370        380        390 
VLTPVQSAGM IPAEPGEAAI KLTSSAGVFS CHPGAPC 

« Hide

References

[1]"Cloning of Amb a I (antigen E), the major allergen family of short ragweed pollen."
Rafnar T., Griffith I.J., Kuo M.-C., Bond J.F., Rogers B.L., Klapper D.G.
J. Biol. Chem. 266:1229-1236(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALLERGEN.
Tissue: Pollen.
[2]"Sequence polymorphism of Amb a I and Amb a II, the major allergens in Ambrosia artemisiifolia (short ragweed)."
Griffith I.J., Pollock J., Klapper D.G., Rogers B.L., Nault A.K.
Int. Arch. Allergy Appl. Immunol. 96:296-304(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS.
Tissue: Pollen.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M62961 mRNA. Translation: AAA32668.1.
M80560 mRNA. Translation: AAA32669.1. Sequence problems.
PIRC39099.
C53240.

3D structure databases

ProteinModelPortalP27761.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

Allergome24. Amb a 1.
789. Amb a 1.0301.
CAZyPL1. Polysaccharide Lyase Family 1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00545; UER00824.

Family and domain databases

Gene3D2.160.20.10. 1 hit.
InterProIPR002022. Amb_allergen_dom.
IPR018082. AmbAllergen.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamPF00544. Pec_lyase_C. 1 hit.
[Graphical view]
PRINTSPR00807. AMBALLERGEN.
SMARTSM00656. Amb_all. 1 hit.
[Graphical view]
SUPFAMSSF51126. SSF51126. 1 hit.
ProtoNetSearch...

Entry information

Entry namePLY2_AMBAR
AccessionPrimary (citable) accession number: P27761
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: June 11, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Allergens

Nomenclature of allergens and list of entries