P27761 (MPA13_AMBAR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Pollen allergen Amb a 1.3 Alternative name(s): Antigen Amb a I Antigen E Short name=AgE Allergen=Amb a 1.3
Organism	Ambrosia artemisiifolia (Short ragweed)
Taxonomic identifier	4212 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › campanulids › Asterales › Asteraceae › Asteroideae › Heliantheae alliance › Heliantheae › Ambrosia

Protein attributes

Sequence length	397 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Monomer.
Tissue specificity	Pollen and flowers.
Post-translational modification	The N-terminus is blocked.
Allergenic properties	Causes an allergic reaction in human. This is one of the major allergens of the ragweed pollen. Ref.1
Sequence similarities	Belongs to the polysaccharide lyase 1 family. Amb a subfamily.

Ontologies

Keywords
Coding sequence diversity	Polymorphism
Disease	Allergen
Domain	Signal
PTM	Disulfide bond
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 397	372	Pollen allergen Amb a 1.3		PRO_0000024903
Amino acid modifications
	Disulfide bond	54 ↔ 71		By similarity
Natural variations
	Natural variant	48	1	L → Y.

Sequences

	Sequence		Length	Mass (Da)
	P27761 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: C8DB41257590DD0A	FASTA	397	42,928
	10 20 30 40 50 60 MGIKQCCYIL YFTLALVALL QPVRSAEGVG EILPSVNETR SLQACEALNI IDKCWRGKAD 70 80 90 100 110 120 WENNRQALAD CAQGFAKGTY GGKWGDVYTV TSNLDDDVAN PKEGTLRFAA AQNRPLWIIF 130 140 150 160 170 180 KNDMVINLNQ ELVVNSDKTI DGRGVKVEII NGGLTLMNVK NIIIHNINIH DVKVLPGGMI 190 200 210 220 230 240 KSNDGPPILR QASDGDTINV AGSSQIWIDH CSLSKSFDGL VDVTLGSTHV TISNCKFTQQ 250 260 270 280 290 300 SKAILLGADD THVQDKGMLA TVAFNMFTDN VDQRMPRCRF GFFQVVNNNY DRWGTYAIGG 310 320 330 340 350 360 SSAPTILCQG NRFLAPDDQI KKNVLARTGT GAAESMAWNW RSDKDLLENG AIFVTSGSDP 370 380 390 VLTPVQSAGM IPAEPGEAAI KLTSSAGVFS CHPGAPC

References

[1]	"Cloning of Amb a I (antigen E), the major allergen family of short ragweed pollen." Rafnar T., Griffith I.J., Kuo M.-C., Bond J.F., Rogers B.L., Klapper D.G. J. Biol. Chem. 266:1229-1236(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALLERGEN. Tissue: Pollen.
[2]	"Sequence polymorphism of Amb a I and Amb a II, the major allergens in Ambrosia artemisiifolia (short ragweed)." Griffith I.J., Pollock J., Klapper D.G., Rogers B.L., Nault A.K. Int. Arch. Allergy Appl. Immunol. 96:296-304(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS. Tissue: Pollen.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M62961 mRNA. Translation: AAA32668.1. M80560 mRNA. Translation: AAA32669.1. Sequence problems.
PIR	C39099. C53240.
3D structure databases
ProteinModelPortal	P27761.
ModBase	Search...
Protein family/group databases
Allergome	24. Amb a 1. 789. Amb a 1.0301.
CAZy	PL1. Polysaccharide Lyase Family 1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	2.160.20.10. 1 hit.
InterPro	IPR002022. Amb_allergen_dom. IPR018082. AmbAllergen. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Pfam	PF00544. Pec_lyase_C. 1 hit. [Graphical view]
PRINTS	PR00807. AMBALLERGEN.
SMART	SM00656. Amb_all. 1 hit. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MPA13_AMBAR

Accession

Primary (citable) accession number: P27761

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	April 3, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program