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Protein

Pectate lyase 1

Gene
N/A
Organism
Ambrosia artemisiifolia (Short ragweed)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Has pectate lyase activity.By similarity

Catalytic activityi

Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi195 – 1951CalciumBy similarity
Metal bindingi219 – 2191CalciumBy similarity
Metal bindingi223 – 2231CalciumBy similarity
Active sitei275 – 2751Sequence Analysis

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. pectate lyase activity Source: UniProtKB-EC

GO - Biological processi

  1. pectin catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00545; UER00824.

Protein family/group databases

CAZyiPL1. Polysaccharide Lyase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Pectate lyase 1 (EC:4.2.2.2)
Alternative name(s):
Antigen Amb a I
Antigen E
Short name:
AgE
Pollen allergen Amb a 1.2
Protein AaBA
Allergen: Amb a 1.2
OrganismiAmbrosia artemisiifolia (Short ragweed)
Taxonomic identifieri4212 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridscampanulidsAsteralesAsteraceaeAsteroideaeHeliantheae allianceHeliantheaeAmbrosia

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human. This is one of the major allergens of the ragweed pollen.1 Publication

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei24. Amb a 1.
788. Amb a 1.0201.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence AnalysisAdd
BLAST
Chaini26 – 398373Pectate lyase 1PRO_0000024902Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi37 – 371N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi55 ↔ 72By similarity

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

Pollen and flowers.

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliP27760.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR002022. Amb_allergen_dom.
IPR018082. AmbAllergen.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00544. Pec_lyase_C. 1 hit.
[Graphical view]
PRINTSiPR00807. AMBALLERGEN.
SMARTiSM00656. Amb_all. 1 hit.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27760-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGIKHCCYIL YFTLALVTLL QPVRSAEDVE EFLPSANETR RSLKACEAHN
60 70 80 90 100
IIDKCWRCKA DWANNRQALA DCAQGFAKGT YGGKHGDVYT VTSDKDDDVA
110 120 130 140 150
NPKEGTLRFA AAQNRPLWII FKRNMVIHLN QELVVNSDKT IDGRGVKVNI
160 170 180 190 200
VNAGLTLMNV KNIIIHNINI HDIKVCPGGM IKSNDGPPIL RQQSDGDAIN
210 220 230 240 250
VAGSSQIWID HCSLSKASDG LLDITLGSSH VTVSNCKFTQ HQFVLLLGAD
260 270 280 290 300
DTHYQDKGML ATVAFNMFTD HVDQRMPRCR FGFFQVVNNN YDRWGTYAIG
310 320 330 340 350
GSSAPTILSQ GNRFFAPDDI IKKNVLARTG TGNAESMSWN WRTDRDLLEN
360 370 380 390
GAIFLPSGSD PVLTPEQKAG MIPAEPGEAV LRLTSSAGVL SCHQGAPC
Length:398
Mass (Da):43,665
Last modified:August 1, 1992 - v1
Checksum:i020DC662D9B7416C
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti345 – 3451R → K.
Natural varianti381 – 3811L → I.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62981 mRNA. Translation: AAA32666.1.
M80559 mRNA. Translation: AAA32667.1.
PIRiB39099.
B53240.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62981 mRNA. Translation: AAA32666.1.
M80559 mRNA. Translation: AAA32667.1.
PIRiB39099.
B53240.

3D structure databases

ProteinModelPortaliP27760.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei24. Amb a 1.
788. Amb a 1.0201.
CAZyiPL1. Polysaccharide Lyase Family 1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00545; UER00824.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR002022. Amb_allergen_dom.
IPR018082. AmbAllergen.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00544. Pec_lyase_C. 1 hit.
[Graphical view]
PRINTSiPR00807. AMBALLERGEN.
SMARTiSM00656. Amb_all. 1 hit.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Cloning of Amb a I (antigen E), the major allergen family of short ragweed pollen."
    Rafnar T., Griffith I.J., Kuo M.-C., Bond J.F., Rogers B.L., Klapper D.G.
    J. Biol. Chem. 266:1229-1236(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALLERGEN.
    Tissue: Pollen.
  2. "Sequence polymorphism of Amb a I and Amb a II, the major allergens in Ambrosia artemisiifolia (short ragweed)."
    Griffith I.J., Pollock J., Klapper D.G., Rogers B.L., Nault A.K.
    Int. Arch. Allergy Appl. Immunol. 96:296-304(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS.
    Tissue: Pollen.
  3. "Isolation and characterization of a new basic antigen from short ragweed pollen (Ambrosia artemisiifolia)."
    Pilyavskaya A., Wieczorek M., Jones S.W., Gross K.
    Mol. Immunol. 32:523-529(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 44-69.

Entry informationi

Entry nameiPLY1_AMBAR
AccessioniPrimary (citable) accession number: P27760
Secondary accession number(s): Q9S8F7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: November 26, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.