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P27760

- PLY1_AMBAR

UniProt

P27760 - PLY1_AMBAR

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Protein
Pectate lyase 1
Gene
N/A
Organism
Ambrosia artemisiifolia (Short ragweed)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Has pectate lyase activity By similarity.

Catalytic activityi

Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.

Cofactori

Binds 1 calcium ion By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi195 – 1951Calcium By similarity
Metal bindingi219 – 2191Calcium By similarity
Metal bindingi223 – 2231Calcium By similarity
Active sitei275 – 2751 Reviewed prediction

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. pectate lyase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. pectin catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00545; UER00824.

Protein family/group databases

CAZyiPL1. Polysaccharide Lyase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Pectate lyase 1 (EC:4.2.2.2)
Alternative name(s):
Antigen Amb a I
Antigen E
Short name:
AgE
Pollen allergen Amb a 1.2
Protein AaBA
Allergen: Amb a 1.2
OrganismiAmbrosia artemisiifolia (Short ragweed)
Taxonomic identifieri4212 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridscampanulidsAsteralesAsteraceaeAsteroideaeHeliantheae allianceHeliantheaeAmbrosia

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human. This is one of the major allergens of the ragweed pollen.1 Publication

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei24. Amb a 1.
788. Amb a 1.0201.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525 Reviewed prediction
Add
BLAST
Chaini26 – 398373Pectate lyase 1
PRO_0000024902Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi37 – 371N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi55 ↔ 72 By similarity

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

Pollen and flowers.

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliP27760.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR002022. Amb_allergen_dom.
IPR018082. AmbAllergen.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00544. Pec_lyase_C. 1 hit.
[Graphical view]
PRINTSiPR00807. AMBALLERGEN.
SMARTiSM00656. Amb_all. 1 hit.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27760-1 [UniParc]FASTAAdd to Basket

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MGIKHCCYIL YFTLALVTLL QPVRSAEDVE EFLPSANETR RSLKACEAHN    50
IIDKCWRCKA DWANNRQALA DCAQGFAKGT YGGKHGDVYT VTSDKDDDVA 100
NPKEGTLRFA AAQNRPLWII FKRNMVIHLN QELVVNSDKT IDGRGVKVNI 150
VNAGLTLMNV KNIIIHNINI HDIKVCPGGM IKSNDGPPIL RQQSDGDAIN 200
VAGSSQIWID HCSLSKASDG LLDITLGSSH VTVSNCKFTQ HQFVLLLGAD 250
DTHYQDKGML ATVAFNMFTD HVDQRMPRCR FGFFQVVNNN YDRWGTYAIG 300
GSSAPTILSQ GNRFFAPDDI IKKNVLARTG TGNAESMSWN WRTDRDLLEN 350
GAIFLPSGSD PVLTPEQKAG MIPAEPGEAV LRLTSSAGVL SCHQGAPC 398
Length:398
Mass (Da):43,665
Last modified:August 1, 1992 - v1
Checksum:i020DC662D9B7416C
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti345 – 3451R → K.
Natural varianti381 – 3811L → I.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M62981 mRNA. Translation: AAA32666.1.
M80559 mRNA. Translation: AAA32667.1.
PIRiB39099.
B53240.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M62981 mRNA. Translation: AAA32666.1 .
M80559 mRNA. Translation: AAA32667.1 .
PIRi B39099.
B53240.

3D structure databases

ProteinModelPortali P27760.
ModBasei Search...

Protein family/group databases

Allergomei 24. Amb a 1.
788. Amb a 1.0201.
CAZyi PL1. Polysaccharide Lyase Family 1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00545 ; UER00824 .

Family and domain databases

Gene3Di 2.160.20.10. 1 hit.
InterProi IPR002022. Amb_allergen_dom.
IPR018082. AmbAllergen.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view ]
Pfami PF00544. Pec_lyase_C. 1 hit.
[Graphical view ]
PRINTSi PR00807. AMBALLERGEN.
SMARTi SM00656. Amb_all. 1 hit.
[Graphical view ]
SUPFAMi SSF51126. SSF51126. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Cloning of Amb a I (antigen E), the major allergen family of short ragweed pollen."
    Rafnar T., Griffith I.J., Kuo M.-C., Bond J.F., Rogers B.L., Klapper D.G.
    J. Biol. Chem. 266:1229-1236(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALLERGEN.
    Tissue: Pollen.
  2. "Sequence polymorphism of Amb a I and Amb a II, the major allergens in Ambrosia artemisiifolia (short ragweed)."
    Griffith I.J., Pollock J., Klapper D.G., Rogers B.L., Nault A.K.
    Int. Arch. Allergy Appl. Immunol. 96:296-304(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS.
    Tissue: Pollen.
  3. "Isolation and characterization of a new basic antigen from short ragweed pollen (Ambrosia artemisiifolia)."
    Pilyavskaya A., Wieczorek M., Jones S.W., Gross K.
    Mol. Immunol. 32:523-529(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 44-69.

Entry informationi

Entry nameiPLY1_AMBAR
AccessioniPrimary (citable) accession number: P27760
Secondary accession number(s): Q9S8F7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: June 11, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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