Reviewed, UniProtKB/Swiss-Prot P27760 (MPA12_AMBAR)

Names and origin

Protein names	Recommended name:     Pollen allergen Amb a 1.2 Alternative name(s):     Antigen Amb a I     Antigen E       Short name=AgE     Protein AaBA     Allergen=Amb a 1.2
Organism	Ambrosia artemisiifolia (Short ragweed)
Taxonomic identifier	4212 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › campanulids › Asterales › Asteraceae › Asteroideae › Heliantheae › Ambrosia

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subunit structure	Monomer.
Tissue specificity	Pollen and flowers.
Post-translational modification	The N-terminus is blocked.
Allergenic properties	Causes an allergic reaction in human. This is one of the major allergens of the ragweed pollen. Ref.1
Sequence similarities	Belongs to the polysaccharide lyase 1 family. Amb a subfamily.

Ontologies

Keywords
Coding sequence diversity	Polymorphism
Disease	Allergen
Domain	Signal
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 398	373	Pollen allergen Amb a 1.2		PRO_0000024902
Amino acid modifications
	Disulfide bond	55 ↔ 72		By similarity
Natural variations
	Natural variant	345	1	R → K
	Natural variant	381	1	L → I

Sequences

	Sequence		Length	Mass (Da)
	P27760-1 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 020DC662D9B7416C	FASTA	398	43,665
	10 20 30 40 50 60 MGIKHCCYIL YFTLALVTLL QPVRSAEDVE EFLPSANETR RSLKACEAHN IIDKCWRCKA 70 80 90 100 110 120 DWANNRQALA DCAQGFAKGT YGGKHGDVYT VTSDKDDDVA NPKEGTLRFA AAQNRPLWII 130 140 150 160 170 180 FKRNMVIHLN QELVVNSDKT IDGRGVKVNI VNAGLTLMNV KNIIIHNINI HDIKVCPGGM 190 200 210 220 230 240 IKSNDGPPIL RQQSDGDAIN VAGSSQIWID HCSLSKASDG LLDITLGSSH VTVSNCKFTQ 250 260 270 280 290 300 HQFVLLLGAD DTHYQDKGML ATVAFNMFTD HVDQRMPRCR FGFFQVVNNN YDRWGTYAIG 310 320 330 340 350 360 GSSAPTILSQ GNRFFAPDDI IKKNVLARTG TGNAESMSWN WRTDRDLLEN GAIFLPSGSD 370 380 390 PVLTPEQKAG MIPAEPGEAV LRLTSSAGVL SCHQGAPC

References

[1]	"Cloning of Amb a I (antigen E), the major allergen family of short ragweed pollen." Rafnar T., Griffith I.J., Kuo M.-C., Bond J.F., Rogers B.L., Klapper D.G. J. Biol. Chem. 266:1229-1236(1991) [PubMed: 1702434] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALLERGENICITY. Tissue: Pollen.
[2]	"Sequence polymorphism of Amb a I and Amb a II, the major allergens in Ambrosia artemisiifolia (short ragweed)." Griffith I.J., Pollock J., Klapper D.G., Rogers B.L., Nault A.K. Int. Arch. Allergy Appl. Immunol. 96:296-304(1991) [PubMed: 1809687] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS. Tissue: Pollen.
[3]	"Isolation and characterization of a new basic antigen from short ragweed pollen (Ambrosia artemisiifolia)." Pilyavskaya A., Wieczorek M., Jones S.W., Gross K. Mol. Immunol. 32:523-529(1995) [PubMed: 7783755] [Abstract] Cited for: PROTEIN SEQUENCE OF 44-69.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M62981 mRNA. Translation: AAA32666.1. M80559 mRNA. Translation: AAA32667.1.
PIR	B39099. B53240.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	PL1. Polysaccharide Lyase Family 1.
Family and domain databases
InterPro	IPR002022. Amb_allergen. IPR018082. AmbAllergen. IPR012334. Pectin_lyas_fold. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00544. Pec_lyase_C. 1 hit. [Graphical view]
PRINTS	PR00807. AMBALLERGEN.
SMART	SM00656. Amb_all. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MPA12_AMBAR

Accession

Primary (citable) accession number: P27760
Secondary accession number(s): Q9S8F7

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	April 14, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)