Reviewed, UniProtKB/Swiss-Prot P27759 (MPA11_AMBAR)

Names and origin

Protein names	Recommended name:     Pollen allergen Amb a 1.1 Alternative name(s):     Antigen Amb a I     Antigen E       Short name=AgE     Allergen=Amb a 1.1
Organism	Ambrosia artemisiifolia (Short ragweed)
Taxonomic identifier	4212 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › campanulids › Asterales › Asteraceae › Asteroideae › Heliantheae › Ambrosia

Protein attributes

Sequence length	396 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subunit structure	Monomer.
Tissue specificity	Pollen and flowers.
Post-translational modification	The N-terminus is blocked.
Allergenic properties	Causes an allergic reaction in human. This is one of the major allergens of the ragweed pollen. Ref.1 Ref.3
Sequence similarities	Belongs to the polysaccharide lyase 1 family. Amb a subfamily.

Ontologies

Keywords
Coding sequence diversity	Polymorphism
Disease	Allergen
Domain	Signal
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 396	371	Pollen allergen Amb a 1.1		PRO_0000024901
Amino acid modifications
	Disulfide bond	53 ↔ 70		By similarity
Natural variations
	Natural variant	92	1	E → D
Experimental info
	Sequence conflict	304	1	T → W AA sequence Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P27759-1 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 0CE7DDECB2B8841D	FASTA	396	42,709
	10 20 30 40 50 60 MGIKHCCYIL YFTLALVTLL QPVRSAEDLQ EILPVNETRR LTTSGAYNII DGCWRGKADW 70 80 90 100 110 120 AENRKALADC AQGFGKGTVG GKDGDIYTVT SELDDDVANP KEGTLRFGAA QNRPLWIIFE 130 140 150 160 170 180 RDMVIRLDKE MVVNSDKTID GRGAKVEIIN AGFTLNGVKN VIIHNINMHD VKVNPGGLIK 190 200 210 220 230 240 SNDGPAAPRA GSDGDAISIS GSSQIWIDHC SLSKSVDGLV DAKLGTTRLT VSNSLFTQHQ 250 260 270 280 290 300 FVLLFGAGDE NIEDRGMLAT VAFNTFTDNV DQRMPRCRHG FFQVVNNNYD KWGSYAIGGS 310 320 330 340 350 360 ASPTILSQGN RFCAPDERSK KNVLGRHGEA AAESMKWNWR TNKDVLENGA IFVASGVDPV 370 380 390 LTPEQSAGMI PAEPGESALS LTSSAGVLSC QPGAPC

References

[1]	"Cloning of Amb a I (antigen E), the major allergen family of short ragweed pollen." Rafnar T., Griffith I.J., Kuo M.-C., Bond J.F., Rogers B.L., Klapper D.G. J. Biol. Chem. 266:1229-1236(1991) [PubMed: 1702434] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, ALLERGENICITY. Tissue: Pollen.
[2]	"Sequence polymorphism of Amb a I and Amb a II, the major allergens in Ambrosia artemisiifolia (short ragweed)." Griffith I.J., Pollock J., Klapper D.G., Rogers B.L., Nault A.K. Int. Arch. Allergy Appl. Immunol. 96:296-304(1991) [PubMed: 1809687] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS. Tissue: Pollen.
[3]	"Monoclonal antibodies to denatured ragweed pollen allergen Amb a I: characterization, specificity for the denatured allergen, and utilization for the isolation of immunogenic peptides of Amb a I." Smith J.J., Olson J.R., Klapper D.G. Mol. Immunol. 25:355-365(1988) [PubMed: 2456454] [Abstract] Cited for: PROTEIN SEQUENCE OF 256-273 AND 292-306, ALLERGENICITY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M63116 mRNA. No translation available. M80558 mRNA. Translation: AAA32665.1.
PIR	A39099.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	PL1. Polysaccharide Lyase Family 1.
Family and domain databases
InterPro	IPR002022. Amb_allergen. IPR018082. AmbAllergen. IPR012334. Pectin_lyas_fold. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00544. Pec_lyase_C. 1 hit. [Graphical view]
PRINTS	PR00807. AMBALLERGEN.
SMART	SM00656. Amb_all. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MPA11_AMBAR

Accession

Primary (citable) accession number: P27759

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	April 14, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)