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P27759

- PLY5_AMBAR

UniProt

P27759 - PLY5_AMBAR

Protein

Pectate lyase 5

Gene
N/A
Organism
Ambrosia artemisiifolia (Short ragweed)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 1 (01 Aug 1992)
      Previous versions | rss
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    Functioni

    Has pectate lyase activity.By similarity

    Catalytic activityi

    Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.

    Cofactori

    Binds 1 calcium ion.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi193 – 1931CalciumBy similarity
    Metal bindingi217 – 2171CalciumBy similarity
    Metal bindingi221 – 2211CalciumBy similarity
    Active sitei273 – 2731Sequence Analysis

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. pectate lyase activity Source: UniProtKB-EC

    GO - Biological processi

    1. pectin catabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00545; UER00824.

    Protein family/group databases

    CAZyiPL1. Polysaccharide Lyase Family 1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pectate lyase 5 (EC:4.2.2.2)
    Alternative name(s):
    Antigen Amb a I
    Antigen E
    Short name:
    AgE
    Pollen allergen Amb a 1.1
    Allergen: Amb a 1.1
    OrganismiAmbrosia artemisiifolia (Short ragweed)
    Taxonomic identifieri4212 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridscampanulidsAsteralesAsteraceaeAsteroideaeHeliantheae allianceHeliantheaeAmbrosia

    Pathology & Biotechi

    Allergenic propertiesi

    Causes an allergic reaction in human. This is one of the major allergens of the ragweed pollen.2 Publications

    Keywords - Diseasei

    Allergen

    Protein family/group databases

    Allergomei24. Amb a 1.
    787. Amb a 1.0101.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2525Sequence AnalysisAdd
    BLAST
    Chaini26 – 396371Pectate lyase 5PRO_0000024901Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi36 – 361N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi53 ↔ 70By similarity

    Post-translational modificationi

    The N-terminus is blocked.

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Expressioni

    Tissue specificityi

    Pollen and flowers.

    Interactioni

    Subunit structurei

    Monomer.

    Structurei

    3D structure databases

    ProteinModelPortaliP27759.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di2.160.20.10. 1 hit.
    InterProiIPR002022. Amb_allergen_dom.
    IPR018082. AmbAllergen.
    IPR012334. Pectin_lyas_fold.
    IPR011050. Pectin_lyase_fold/virulence.
    [Graphical view]
    PfamiPF00544. Pec_lyase_C. 1 hit.
    [Graphical view]
    PRINTSiPR00807. AMBALLERGEN.
    SMARTiSM00656. Amb_all. 1 hit.
    [Graphical view]
    SUPFAMiSSF51126. SSF51126. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P27759-1 [UniParc]FASTAAdd to Basket

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    MGIKHCCYIL YFTLALVTLL QPVRSAEDLQ EILPVNETRR LTTSGAYNII    50
    DGCWRGKADW AENRKALADC AQGFGKGTVG GKDGDIYTVT SELDDDVANP 100
    KEGTLRFGAA QNRPLWIIFE RDMVIRLDKE MVVNSDKTID GRGAKVEIIN 150
    AGFTLNGVKN VIIHNINMHD VKVNPGGLIK SNDGPAAPRA GSDGDAISIS 200
    GSSQIWIDHC SLSKSVDGLV DAKLGTTRLT VSNSLFTQHQ FVLLFGAGDE 250
    NIEDRGMLAT VAFNTFTDNV DQRMPRCRHG FFQVVNNNYD KWGSYAIGGS 300
    ASPTILSQGN RFCAPDERSK KNVLGRHGEA AAESMKWNWR TNKDVLENGA 350
    IFVASGVDPV LTPEQSAGMI PAEPGESALS LTSSAGVLSC QPGAPC 396
    Length:396
    Mass (Da):42,709
    Last modified:August 1, 1992 - v1
    Checksum:i0CE7DDECB2B8841D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti304 – 3041T → W AA sequence (PubMed:2456454)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti92 – 921E → D.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M63116 mRNA. No translation available.
    M80558 mRNA. Translation: AAA32665.1.
    PIRiA39099.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M63116 mRNA. No translation available.
    M80558 mRNA. Translation: AAA32665.1 .
    PIRi A39099.

    3D structure databases

    ProteinModelPortali P27759.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    Allergomei 24. Amb a 1.
    787. Amb a 1.0101.
    CAZyi PL1. Polysaccharide Lyase Family 1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00545 ; UER00824 .

    Family and domain databases

    Gene3Di 2.160.20.10. 1 hit.
    InterProi IPR002022. Amb_allergen_dom.
    IPR018082. AmbAllergen.
    IPR012334. Pectin_lyas_fold.
    IPR011050. Pectin_lyase_fold/virulence.
    [Graphical view ]
    Pfami PF00544. Pec_lyase_C. 1 hit.
    [Graphical view ]
    PRINTSi PR00807. AMBALLERGEN.
    SMARTi SM00656. Amb_all. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51126. SSF51126. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of Amb a I (antigen E), the major allergen family of short ragweed pollen."
      Rafnar T., Griffith I.J., Kuo M.-C., Bond J.F., Rogers B.L., Klapper D.G.
      J. Biol. Chem. 266:1229-1236(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, ALLERGEN.
      Tissue: Pollen.
    2. "Sequence polymorphism of Amb a I and Amb a II, the major allergens in Ambrosia artemisiifolia (short ragweed)."
      Griffith I.J., Pollock J., Klapper D.G., Rogers B.L., Nault A.K.
      Int. Arch. Allergy Appl. Immunol. 96:296-304(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS.
      Tissue: Pollen.
    3. "Monoclonal antibodies to denatured ragweed pollen allergen Amb a I: characterization, specificity for the denatured allergen, and utilization for the isolation of immunogenic peptides of Amb a I."
      Smith J.J., Olson J.R., Klapper D.G.
      Mol. Immunol. 25:355-365(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 256-273 AND 292-306, ALLERGEN.

    Entry informationi

    Entry nameiPLY5_AMBAR
    AccessioniPrimary (citable) accession number: P27759
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: August 1, 1992
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Allergens
      Nomenclature of allergens and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3