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Protein

Pectate lyase 5

Gene
N/A
Organism
Ambrosia artemisiifolia (Short ragweed)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Has pectate lyase activity.By similarity

Catalytic activityi

Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi193 – 1931CalciumBy similarity
Metal bindingi217 – 2171CalciumBy similarity
Metal bindingi221 – 2211CalciumBy similarity
Active sitei273 – 2731Sequence Analysis

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. pectate lyase activity Source: UniProtKB-EC

GO - Biological processi

  1. pectin catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00545; UER00824.

Protein family/group databases

CAZyiPL1. Polysaccharide Lyase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Pectate lyase 5 (EC:4.2.2.2)
Alternative name(s):
Antigen Amb a I
Antigen E
Short name:
AgE
Pollen allergen Amb a 1.1
Allergen: Amb a 1.1
OrganismiAmbrosia artemisiifolia (Short ragweed)
Taxonomic identifieri4212 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridscampanulidsAsteralesAsteraceaeAsteroideaeHeliantheae allianceHeliantheaeAmbrosia

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human. This is one of the major allergens of the ragweed pollen.2 Publications

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei24. Amb a 1.
787. Amb a 1.0101.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence AnalysisAdd
BLAST
Chaini26 – 396371Pectate lyase 5PRO_0000024901Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi36 – 361N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi53 ↔ 70By similarity

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

Pollen and flowers.

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliP27759.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR002022. Amb_allergen_dom.
IPR018082. AmbAllergen.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00544. Pec_lyase_C. 1 hit.
[Graphical view]
PRINTSiPR00807. AMBALLERGEN.
SMARTiSM00656. Amb_all. 1 hit.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27759-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGIKHCCYIL YFTLALVTLL QPVRSAEDLQ EILPVNETRR LTTSGAYNII
60 70 80 90 100
DGCWRGKADW AENRKALADC AQGFGKGTVG GKDGDIYTVT SELDDDVANP
110 120 130 140 150
KEGTLRFGAA QNRPLWIIFE RDMVIRLDKE MVVNSDKTID GRGAKVEIIN
160 170 180 190 200
AGFTLNGVKN VIIHNINMHD VKVNPGGLIK SNDGPAAPRA GSDGDAISIS
210 220 230 240 250
GSSQIWIDHC SLSKSVDGLV DAKLGTTRLT VSNSLFTQHQ FVLLFGAGDE
260 270 280 290 300
NIEDRGMLAT VAFNTFTDNV DQRMPRCRHG FFQVVNNNYD KWGSYAIGGS
310 320 330 340 350
ASPTILSQGN RFCAPDERSK KNVLGRHGEA AAESMKWNWR TNKDVLENGA
360 370 380 390
IFVASGVDPV LTPEQSAGMI PAEPGESALS LTSSAGVLSC QPGAPC
Length:396
Mass (Da):42,709
Last modified:August 1, 1992 - v1
Checksum:i0CE7DDECB2B8841D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti304 – 3041T → W AA sequence (PubMed:2456454)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti92 – 921E → D.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63116 mRNA. No translation available.
M80558 mRNA. Translation: AAA32665.1.
PIRiA39099.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63116 mRNA. No translation available.
M80558 mRNA. Translation: AAA32665.1.
PIRiA39099.

3D structure databases

ProteinModelPortaliP27759.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei24. Amb a 1.
787. Amb a 1.0101.
CAZyiPL1. Polysaccharide Lyase Family 1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00545; UER00824.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR002022. Amb_allergen_dom.
IPR018082. AmbAllergen.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00544. Pec_lyase_C. 1 hit.
[Graphical view]
PRINTSiPR00807. AMBALLERGEN.
SMARTiSM00656. Amb_all. 1 hit.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Cloning of Amb a I (antigen E), the major allergen family of short ragweed pollen."
    Rafnar T., Griffith I.J., Kuo M.-C., Bond J.F., Rogers B.L., Klapper D.G.
    J. Biol. Chem. 266:1229-1236(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, ALLERGEN.
    Tissue: Pollen.
  2. "Sequence polymorphism of Amb a I and Amb a II, the major allergens in Ambrosia artemisiifolia (short ragweed)."
    Griffith I.J., Pollock J., Klapper D.G., Rogers B.L., Nault A.K.
    Int. Arch. Allergy Appl. Immunol. 96:296-304(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS.
    Tissue: Pollen.
  3. "Monoclonal antibodies to denatured ragweed pollen allergen Amb a I: characterization, specificity for the denatured allergen, and utilization for the isolation of immunogenic peptides of Amb a I."
    Smith J.J., Olson J.R., Klapper D.G.
    Mol. Immunol. 25:355-365(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 256-273 AND 292-306, ALLERGEN.

Entry informationi

Entry nameiPLY5_AMBAR
AccessioniPrimary (citable) accession number: P27759
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: November 26, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.