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P27759 (PLY5_AMBAR) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pectate lyase 5

EC=4.2.2.2
Alternative name(s):
Antigen Amb a I
Antigen E
Short name=AgE
Pollen allergen Amb a 1.1
Allergen=Amb a 1.1
OrganismAmbrosia artemisiifolia (Short ragweed)
Taxonomic identifier4212 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridscampanulidsAsteralesAsteraceaeAsteroideaeHeliantheae allianceHeliantheaeAmbrosia

Protein attributes

Sequence length396 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Has pectate lyase activity By similarity.

Catalytic activity

Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.

Cofactor

Binds 1 calcium ion By similarity.

Pathway

Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 2/5.

Subunit structure

Monomer.

Tissue specificity

Pollen and flowers.

Post-translational modification

The N-terminus is blocked.

Allergenic properties

Causes an allergic reaction in human. This is one of the major allergens of the ragweed pollen. Ref.1 Ref.3

Sequence similarities

Belongs to the polysaccharide lyase 1 family. Amb a subfamily.

Ontologies

Keywords
   Coding sequence diversityPolymorphism
   DiseaseAllergen
   DomainSignal
   LigandCalcium
Metal-binding
   Molecular functionLyase
   PTMDisulfide bond
Glycoprotein
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processpectin catabolic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

pectate lyase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Chain26 – 396371Pectate lyase 5
PRO_0000024901

Sites

Active site2731 Potential
Metal binding1931Calcium By similarity
Metal binding2171Calcium By similarity
Metal binding2211Calcium By similarity

Amino acid modifications

Glycosylation361N-linked (GlcNAc...) Potential
Disulfide bond53 ↔ 70 By similarity

Natural variations

Natural variant921E → D.

Experimental info

Sequence conflict3041T → W AA sequence Ref.3

Sequences

Sequence LengthMass (Da)Tools
P27759 [UniParc].

Last modified August 1, 1992. Version 1.
Checksum: 0CE7DDECB2B8841D

FASTA39642,709
        10         20         30         40         50         60 
MGIKHCCYIL YFTLALVTLL QPVRSAEDLQ EILPVNETRR LTTSGAYNII DGCWRGKADW 

        70         80         90        100        110        120 
AENRKALADC AQGFGKGTVG GKDGDIYTVT SELDDDVANP KEGTLRFGAA QNRPLWIIFE 

       130        140        150        160        170        180 
RDMVIRLDKE MVVNSDKTID GRGAKVEIIN AGFTLNGVKN VIIHNINMHD VKVNPGGLIK 

       190        200        210        220        230        240 
SNDGPAAPRA GSDGDAISIS GSSQIWIDHC SLSKSVDGLV DAKLGTTRLT VSNSLFTQHQ 

       250        260        270        280        290        300 
FVLLFGAGDE NIEDRGMLAT VAFNTFTDNV DQRMPRCRHG FFQVVNNNYD KWGSYAIGGS 

       310        320        330        340        350        360 
ASPTILSQGN RFCAPDERSK KNVLGRHGEA AAESMKWNWR TNKDVLENGA IFVASGVDPV 

       370        380        390 
LTPEQSAGMI PAEPGESALS LTSSAGVLSC QPGAPC 

« Hide

References

[1]"Cloning of Amb a I (antigen E), the major allergen family of short ragweed pollen."
Rafnar T., Griffith I.J., Kuo M.-C., Bond J.F., Rogers B.L., Klapper D.G.
J. Biol. Chem. 266:1229-1236(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, ALLERGEN.
Tissue: Pollen.
[2]"Sequence polymorphism of Amb a I and Amb a II, the major allergens in Ambrosia artemisiifolia (short ragweed)."
Griffith I.J., Pollock J., Klapper D.G., Rogers B.L., Nault A.K.
Int. Arch. Allergy Appl. Immunol. 96:296-304(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS.
Tissue: Pollen.
[3]"Monoclonal antibodies to denatured ragweed pollen allergen Amb a I: characterization, specificity for the denatured allergen, and utilization for the isolation of immunogenic peptides of Amb a I."
Smith J.J., Olson J.R., Klapper D.G.
Mol. Immunol. 25:355-365(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 256-273 AND 292-306, ALLERGEN.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M63116 mRNA. No translation available.
M80558 mRNA. Translation: AAA32665.1.
PIRA39099.

3D structure databases

ProteinModelPortalP27759.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

Allergome24. Amb a 1.
787. Amb a 1.0101.
CAZyPL1. Polysaccharide Lyase Family 1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00545; UER00824.

Family and domain databases

Gene3D2.160.20.10. 1 hit.
InterProIPR002022. Amb_allergen_dom.
IPR018082. AmbAllergen.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamPF00544. Pec_lyase_C. 1 hit.
[Graphical view]
PRINTSPR00807. AMBALLERGEN.
SMARTSM00656. Amb_all. 1 hit.
[Graphical view]
SUPFAMSSF51126. SSF51126. 1 hit.
ProtoNetSearch...

Entry information

Entry namePLY5_AMBAR
AccessionPrimary (citable) accession number: P27759
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: June 11, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Allergens

Nomenclature of allergens and list of entries