P27756 (AGAL_STRMU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-galactosidase
EC=3.2.1.22

Alternative name(s):
Melibiase

Gene names

Name:	aga
Synonyms:	agaL
Ordered Locus Names:	SMU_877

Organism

Streptococcus mutans [Complete proteome] [HAMAP]

Taxonomic identifier

1309 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	720 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.
Cofactor	Magnesium. NAD.
Subunit structure	Homotetramer.
Sequence similarities	Belongs to the glycosyl hydrolase 36 family.

Ontologies

Keywords
Ligand	Magnesium NAD
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	raffinose alpha-galactosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 720	720	Alpha-galactosidase		PRO_0000134876
Sites
	Active site	472	1	Nucleophile By similarity
	Active site	512	1	Proton donor By similarity
Experimental info
	Sequence conflict	138 – 143	6	LRMTLY → SRIVSD in AAA26933. Ref.1
	Sequence conflict	198	1	T → S in AAA26933. Ref.1
	Sequence conflict	262	1	E → V in AAA26933. Ref.1
	Sequence conflict	277	1	D → E in AAA26933. Ref.1
	Sequence conflict	447	1	P → L in AAA26933. Ref.1
	Sequence conflict	502 – 504	3	NIV → DMF in AAA26933. Ref.1
	Sequence conflict	586	1	G → A in AAA26933. Ref.1
	Sequence conflict	602	1	I → V in AAA26933. Ref.1
	Sequence conflict	690	1	I → A in AAA26933. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P27756 [UniParc]. Last modified November 28, 2002. Version 3. Checksum: ED2ABF54ACFC5247	FASTA	720	82,141
	10 20 30 40 50 60 MGIVIKDNLF YIHTKDSSLI IEERDGDLLL KHLGKKIEAY HFSNTVFEKD HAFSANPVAD 70 80 90 100 110 120 NRNYSYDTQR QIFGVHGFGD FRVPSLILQH DNNDLTRFKF KKAKIIKGGI KARGLPNPHS 130 140 150 160 170 180 SESAQSLALI LEDDLAKLRM TLYYTAYDDN ATISTFVKFE NLSDKPVILH RALSTMFDLP 190 200 210 220 230 240 ASHYDVITFQ GAYAREKTLR RHQIEQGLFK IGSNRGASGH AQTPSLILTE HDSNEFYGEA 250 260 270 280 290 300 LALQLIYSGN FQAFVQKNQL NEVRLGIGIN DDNFSWDLQA NQSFETPVAL ITYTDKGLTD 310 320 330 340 350 360 LTQESHNFIK RHIIPKNFAN KERPILINNW EATYFDFNRS QLLGLADEAR KLGIELFVLD 370 380 390 400 410 420 DGWFGHRFDD NSSLGDWFVN EEKLGGSLDS FIKEIHDRGL QFGLWFEPEM VSVDSKLYRA 430 440 450 460 470 480 HPDWVIQADQ REHTYSRNQL VLNLANPDVV AYIKTVLDKL LTENTIDYVK WDYNRNITNI 490 500 510 520 530 540 GNGRTYLETQ MQSHAYILGL YNIVSYLTTK HDKVLFESCS GGGGRNDLGM MCYFPQVWSS 550 560 570 580 590 600 DNTDAIARLP IQYGSSYLYP TISMGAHVSA VPNHQMNRHT PLTTRGHVAM MGNLGYELDL 610 620 630 640 650 660 AILTKNEKKA VANQIKHYKK IRSVVQFGKL YRLINPEVGI NEVAVQYTYD NQVLVTYVRI 670 680 690 700 710 720 QSTIEMMETT VKLRGLDADA IYVLIETGQI FSGAELMYAG LTIDMPQGDY LSKQYYFIKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Biochemical and genetic analysis of Streptococcus mutans alpha-galactosidase." Aduse-Opoku J., Tao L., Ferretti J.J., Russell R.R.B. J. Gen. Microbiol. 137:757-764(1991) [PubMed: 1649890] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Ingbritt.
[2]	"Biochemical and genetic analysis of Streptococcus mutans alpha-galactosidase." Aduse-Opoku J., Tao L., Ferretti J.J., Russell R.R.B. J. Gen. Microbiol. 137:2271-2272(1991) [PubMed: 1660918] [Abstract] Cited for: SEQUENCE REVISION.
[3]	"Genome sequence of Streptococcus mutans UA159, a cariogenic dental pathogen." Ajdic D.J., McShan W.M., McLaughlin R.E., Savic G., Chang J., Carson M.B., Primeaux C., Tian R., Kenton S., Jia H.G., Lin S.P., Qian Y., Li S., Zhu H., Najar F.Z., Lai H., White J., Roe B.A., Ferretti J.J. Proc. Natl. Acad. Sci. U.S.A. 99:14434-14439(2002) [PubMed: 12397186] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700610 / UA159 / Serotype c.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M77351 Genomic DNA. Translation: AAA26933.1. AE014133 Genomic DNA. Translation: AAN58592.1.
PIR	A44819.
RefSeq	NP_721286.1. NC_004350.2.
3D structure databases
ProteinModelPortal	P27756.
ModBase	Search...
Protein family/group databases
CAZy	GH36. Glycoside Hydrolase Family 36.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTRT00000013976; EBSTRP00000013458; EBSTRG00000013976.
GeneID	1028243.
GenomeReviews	Gene locus SMU_877 in contig AE014133_GR.
KEGG	smu:SMU_877.
NMPDR	fig|210007.1.peg.799.
PATRIC	19663901. VBIStrMut61772_0783.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000027989.
HOGENOM	HBG302872.
OMA	RNDLGMM.
ProtClustDB	CLSK626013.
Enzyme and pathway databases
BioCyc	SMUT210007:SMU_877-MONOMER.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR002252. Glyco_hydro_36. IPR000111. Glyco_hydro_GHD. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K07407.
Pfam	PF02065. Melibiase. 1 hit. [Graphical view]
PIRSF	PIRSF005536. Agal. 1 hit.
PRINTS	PR00743. GLHYDRLASE36.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00512. ALPHA_GALACTOSIDASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AGAL_STRMU

Accession

Primary (citable) accession number: P27756

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	November 28, 2002
Last modified:	January 25, 2012
This is version 83 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program