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Reviewed, UniProtKB/Swiss-Prot P27756 (AGAL_STRMU)

Last modified November 3, 2009. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Alpha-galactosidase
    EC=3.2.1.22
Alternative name(s):
    Melibiase
Gene names
Name: aga
Synonyms: agaL
Ordered Locus Names: SMU_877
OrganismStreptococcus mutans [Complete proteome] [HAMAP]
Taxonomic identifier1309 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

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Protein attributes

Sequence length720 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Catalytic activity

Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactohydrolase.

Cofactor

Magnesium.

NAD.

Subunit structure

Homotetramer.

Sequence similarities

Belongs to the glycosyl hydrolase 36 family.

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Ontologies

Keywords
   LigandMagnesium
NAD
   Molecular functionGlycosidase
Hydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular functionalpha-galactosidase activity

Inferred from electronic annotation. Source: EC

magnesium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 720720Alpha-galactosidase
PRO_0000134876

Sites

Active site4721Nucleophile By similarity
Active site5121Proton donor By similarity

Experimental info

Sequence conflict138 – 1436LRMTLY → SRIVSD in AAA26933. Ref.1
Sequence conflict1981T → S in AAA26933. Ref.1
Sequence conflict2621E → V in AAA26933. Ref.1
Sequence conflict2771D → E in AAA26933. Ref.1
Sequence conflict4471P → L in AAA26933. Ref.1
Sequence conflict502 – 5043NIV → DMF in AAA26933. Ref.1
Sequence conflict5861G → A in AAA26933. Ref.1
Sequence conflict6021I → V in AAA26933. Ref.1
Sequence conflict6901I → A in AAA26933. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
P27756-1 [UniParc].

Last modified November 28, 2002. Version 3.
Checksum: ED2ABF54ACFC5247

FASTA72082,141
        10         20         30         40         50         60 
MGIVIKDNLF YIHTKDSSLI IEERDGDLLL KHLGKKIEAY HFSNTVFEKD HAFSANPVAD 

        70         80         90        100        110        120 
NRNYSYDTQR QIFGVHGFGD FRVPSLILQH DNNDLTRFKF KKAKIIKGGI KARGLPNPHS 

       130        140        150        160        170        180 
SESAQSLALI LEDDLAKLRM TLYYTAYDDN ATISTFVKFE NLSDKPVILH RALSTMFDLP 

       190        200        210        220        230        240 
ASHYDVITFQ GAYAREKTLR RHQIEQGLFK IGSNRGASGH AQTPSLILTE HDSNEFYGEA 

       250        260        270        280        290        300 
LALQLIYSGN FQAFVQKNQL NEVRLGIGIN DDNFSWDLQA NQSFETPVAL ITYTDKGLTD 

       310        320        330        340        350        360 
LTQESHNFIK RHIIPKNFAN KERPILINNW EATYFDFNRS QLLGLADEAR KLGIELFVLD 

       370        380        390        400        410        420 
DGWFGHRFDD NSSLGDWFVN EEKLGGSLDS FIKEIHDRGL QFGLWFEPEM VSVDSKLYRA 

       430        440        450        460        470        480 
HPDWVIQADQ REHTYSRNQL VLNLANPDVV AYIKTVLDKL LTENTIDYVK WDYNRNITNI 

       490        500        510        520        530        540 
GNGRTYLETQ MQSHAYILGL YNIVSYLTTK HDKVLFESCS GGGGRNDLGM MCYFPQVWSS 

       550        560        570        580        590        600 
DNTDAIARLP IQYGSSYLYP TISMGAHVSA VPNHQMNRHT PLTTRGHVAM MGNLGYELDL 

       610        620        630        640        650        660 
AILTKNEKKA VANQIKHYKK IRSVVQFGKL YRLINPEVGI NEVAVQYTYD NQVLVTYVRI 

       670        680        690        700        710        720 
QSTIEMMETT VKLRGLDADA IYVLIETGQI FSGAELMYAG LTIDMPQGDY LSKQYYFIKK

« Hide

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References

« Hide 'large scale' references
[1]"Biochemical and genetic analysis of Streptococcus mutans alpha-galactosidase."
Aduse-Opoku J., Tao L., Ferretti J.J., Russell R.R.B.
J. Gen. Microbiol. 137:757-764(1991) [PubMed: 1649890] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Ingbritt.
[2]"Biochemical and genetic analysis of Streptococcus mutans alpha-galactosidase."
Aduse-Opoku J., Tao L., Ferretti J.J., Russell R.R.B.
J. Gen. Microbiol. 137:2271-2272(1991) [PubMed: 1660918] [Abstract]
Cited for: SEQUENCE REVISION.
[3]"Genome sequence of Streptococcus mutans UA159, a cariogenic dental pathogen."
Ajdic D.J., McShan W.M., McLaughlin R.E., Savic G., Chang J., Carson M.B., Primeaux C., Tian R., Kenton S., Jia H.G., Lin S.P., Qian Y., Li S., Zhu H., Najar F.Z., Lai H., White J., Roe B.A., Ferretti J.J.
Proc. Natl. Acad. Sci. U.S.A. 99:14434-14439(2002) [PubMed: 12397186] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700610 / UA159 / Serotype c.

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Cross-references

Sequence databases

M77351 Genomic DNA. Translation: AAA26933.1.
AE014133 Genomic DNA. Translation: AAN58592.1.
PIRA44819.
RefSeqNP_721286.1.

3D structure databases

ModBaseSearch...

Protein family/group databases

CAZyGH36. Glycoside Hydrolase Family 36.

Genome annotation databases

GeneID1028243.
GenomeReviewsGene locus SMU_877 in contig AE014133_GR.
KEGGsmu:SMU.877.
NMPDRfig|210007.1.peg.799.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMP27756.
OMARNDLGMM.

Enzyme and pathway databases

BioCycSMUT210007:SMU_877-MON.
BRENDA3.2.1.22. 20716.

Family and domain databases

InterProIPR002252. Glyco_hydro_36.
IPR000111. Glyco_hydro_GHD.
[Graphical view]
PfamPF02065. Melibiase. 1 hit.
[Graphical view]
PIRSFPIRSF005536. Agal. 1 hit.
PRINTSPR00743. GLHYDRLASE36.
PROSITEPS00512. ALPHA_GALACTOSIDASE. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameAGAL_STRMU
AccessionPrimary (citable) accession number: P27756
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: November 28, 2002
Last modified: November 3, 2009
This is version 64 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents